BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	645,034	A→G	V257A (GTG→GCG)	ybdS ←	YbdS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	645,034	0	A	G	99.7%	978.5 / NA	306	V257A (GTG→GCG)	ybdS	YbdS
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (143/161); minor base C (0/1); total (143/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

TCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACC  >  NC_000913/644944‑645122

tctctGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGcgcg                                                                                        >  2:7603678/1‑93 (MQ=255)

  tctGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTa                                                                                      >  2:19831/1‑93 (MQ=255)

  tctGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTCCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTa                                                                                      <  1:9000743/93‑1 (MQ=255)

    tGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAgg                                                                                    >  1:3412870/1‑93 (MQ=255)

    tGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAgg                                                                                    >  2:4138488/1‑93 (MQ=255)

     gcgcGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACCGCGCGTAGGa                                                                                   >  2:2824098/1‑93 (MQ=255)

     gCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGa                                                                                   <  1:5716472/93‑1 (MQ=255)

        tgtCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAg                                                                                >  2:5931264/1‑93 (MQ=255)

           cAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCa                                                                             <  2:5087902/93‑1 (MQ=255)

           cAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCa                                                                             <  2:3950717/93‑1 (MQ=255)

            aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCAc                                                                            <  2:4842075/93‑1 (MQ=255)

            aGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCAc                                                                            <  1:942751/93‑1 (MQ=255)

             gcgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACg                                                                           <  2:9042574/93‑1 (MQ=255)

              cgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACgg                                                                          <  1:6410375/93‑1 (MQ=255)

              cgcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACgg                                                                          <  1:2296377/93‑1 (MQ=255)

               gcACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGgc                                                                         >  2:2331936/1‑93 (MQ=255)

                  cccATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCa                                                                      >  1:3959938/1‑93 (MQ=255)

                  cccATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCa                                                                      >  1:1617904/1‑93 (MQ=255)

                   ccATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCaa                                                                     <  2:5300914/93‑1 (MQ=255)

                    cATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAAt                                                                    >  1:2569156/1‑93 (MQ=255)

                     ctGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTTTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATg                                                                   <  1:2838645/92‑1 (MQ=255)

                      tGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGa                                                                  <  1:760850/93‑1 (MQ=255)

                      tGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGACCAATGa                                                                  >  2:5745372/1‑93 (MQ=255)

                        gTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATa                                                                >  1:9592977/1‑93 (MQ=255)

                         ttttCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATaa                                                               >  2:3512917/1‑93 (MQ=255)

                          tttCAGTTCATCACGCGCCCAGGTTGCCTCTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAg                                                              <  1:15458/93‑1 (MQ=255)

                          tttCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATaac                                                              >  1:7354880/1‑92 (MQ=255)

                          tttCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAg                                                              <  2:2509567/93‑1 (MQ=255)

                          tttCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAg                                                              >  2:6909932/1‑93 (MQ=255)

                          tttCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAg                                                              >  2:7745436/1‑93 (MQ=255)

                            tCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCa                                                            >  2:9709818/1‑93 (MQ=255)

                              aGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAg                                                          >  2:3865931/1‑93 (MQ=255)

                               gTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGa                                                         >  2:6128865/1‑93 (MQ=255)

                                ttCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGAt                                                        <  1:3310966/93‑1 (MQ=255)

                                ttCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGAt                                                        >  1:1087864/1‑93 (MQ=255)

                                 tcatcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATa                                                       <  1:4167795/93‑1 (MQ=255)

                                   atcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAAc                                                     >  1:4766684/1‑93 (MQ=255)

                                   atcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAAc                                                     <  1:2232915/93‑1 (MQ=255)

                                   atcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAAc                                                     <  2:9143142/93‑1 (MQ=255)

                                    tcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAAcc                                                    >  2:5544321/1‑93 (MQ=255)

                                    tcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAAcc                                                    <  2:9345906/93‑1 (MQ=255)

                                    tcaCCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAAcc                                                    <  1:874913/93‑1 (MQ=255)

                                       cccGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACcaca                                                 >  1:6588877/1‑93 (MQ=255)

                                        ccGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCaa                                                <  1:8010596/93‑1 (MQ=255)

                                         cGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAAc                                               >  1:132705/1‑93 (MQ=255)

                                         cGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAAc                                               <  2:988944/93‑1 (MQ=255)

                                         cGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAAc                                               <  1:4349098/93‑1 (MQ=255)

                                         cGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAAc                                               <  1:1674410/93‑1 (MQ=255)

                                           cccAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCg                                             >  1:1935986/1‑93 (MQ=255)

                                            ccAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCgg                                            <  2:8646867/93‑1 (MQ=255)

                                             cAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGc                                           >  1:3010387/1‑93 (MQ=255)

                                               ggTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAg                                         <  1:8214381/93‑1 (MQ=255)

                                                gTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAgg                                        <  2:4568014/93‑1 (MQ=255)

                                                gTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAgg                                        <  1:3390778/93‑1 (MQ=255)

                                                gTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAgg                                        >  2:1223497/1‑93 (MQ=255)

                                                    ccACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGc                                    <  2:3783076/93‑1 (MQ=255)

                                                      aCTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCag                                  <  2:353759/93‑1 (MQ=255)

                                                       cttcttCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCaga                                 <  1:3587502/93‑1 (MQ=255)

                                                       cttcttCACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCaga                                 >  1:6861380/1‑93 (MQ=255)

                                                        ttcttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCagag                                >  1:621803/1‑93 (MQ=255)

                                                        tgcttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCagag                                <  2:315252/91‑1 (MQ=255)

                                                         tcttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAgg                               >  1:4617019/1‑93 (MQ=255)

                                                         tcttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAgg                               <  1:5231890/93‑1 (MQ=255)

                                                         tcttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAgg                               >  1:2753813/1‑93 (MQ=255)

                                                          cttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGa                              >  2:5630357/1‑93 (MQ=255)

                                                          cttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGa                              <  2:6614533/93‑1 (MQ=255)

                                                           ttcACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGaa                             <  2:7177355/93‑1 (MQ=255)

                                                             cACTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAAcc                           <  1:2412106/93‑1 (MQ=255)

                                                              aCTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCa                          <  2:853611/93‑1 (MQ=255)

                                                              aCTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCa                          <  2:4980114/93‑1 (MQ=255)

                                                              aCTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCa                          >  1:8645510/1‑93 (MQ=255)

                                                               cTGTGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCAc                         <  1:7081843/93‑1 (MQ=255)

                                                               cTGGGTGTGATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCAc                         >  2:735772/1‑93 (MQ=255)

                                                                tgtgtgtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACt                        >  2:2735958/1‑93 (MQ=255)

                                                                tgtgtgtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACt                        >  2:5199543/1‑93 (MQ=255)

                                                                  tgtgtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGc                      >  2:8021050/1‑93 (MQ=255)

                                                                    tgtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCaa                    <  1:2724215/93‑1 (MQ=255)

                                                                    tgtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCaa                    >  1:9285328/1‑93 (MQ=255)

                                                                     gtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAAc                   <  2:7101989/93‑1 (MQ=255)

                                                                     gtgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAAc                   >  1:6675680/1‑93 (MQ=255)

                                                                      tgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAAcc                  <  1:7778681/93‑1 (MQ=255)

                                                                      tgATTTCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAAcc                  >  2:4378347/1‑93 (MQ=255)

                                                                          ttCCGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCt              <  2:1573246/93‑1 (MQ=255)

                                                                            ccGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACGCCAACCGGCAGGGAGCAGAGCAACCACTGCAACCAGCTaa            >  1:5310471/1‑93 (MQ=255)

                                                                            ccGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTaa            <  2:8121242/93‑1 (MQ=255)

                                                                            ccGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTaa            <  2:8883204/93‑1 (MQ=255)

                                                                             cGGTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAAt           >  1:2096226/1‑93 (MQ=255)

                                                                              ggTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATc          <  2:9450539/93‑1 (MQ=255)

                                                                              ggTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATc          <  1:989572/93‑1 (MQ=255)

                                                                              ggTTTGTACAGCCGGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATc          <  1:324702/93‑1 (MQ=255)

                                                                               gTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCt         <  2:3195700/93‑1 (MQ=255)

                                                                               gTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCt         >  2:2948599/1‑93 (MQ=255)

                                                                               gTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCt         >  1:3841380/1‑93 (MQ=255)

                                                                               gTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCt         >  1:2004388/1‑93 (MQ=255)

                                                                               gTTTGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCt         <  1:4275921/93‑1 (MQ=255)

                                                                                  tGTACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGa      <  2:217874/93‑1 (MQ=255)

                                                                                    tACAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATa    <  2:8927886/93‑1 (MQ=255)

                                                                                     aCAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATAc   <  1:6042092/93‑1 (MQ=255)

                                                                                      cAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATAcc  <  2:3371603/93‑1 (MQ=255)

                                                                                      cAGCGCGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATAcc  <  1:5947549/93‑1 (MQ=255)

TCTCTGCGTGTCAGCGCACCCATGGTTTTCAGTTCATCACCCGCCCAGGTTGCCACTTCTTCACTGTGTGTGATTTCCGGTTTGTACAGCACGTAGGAAAGCCACGGCGCAATGATAAGCAAGATAACCCCAACCGGCAGGAAGCAGAGGAACCACTGCAACCAGCTAATCTGGATACC  >  NC_000913/644944‑645122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`