BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,914	C→T	G365S (GGC→AGC)	leuS ←	leucine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,914	0	C	T	99.3%	1044.0 / ‑4.4	290	G365S (GGC→AGC)	leuS	leucine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (158/130); total (158/132)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 292 total aligned reads displayed.

CCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATAA  >  NC_000913/672824‑673003

ccGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGt                                                                                         <  1:8293065/93‑1 (MQ=255)

 cGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTc                                                                                        <  2:3533953/93‑1 (MQ=255)

  gCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCa                                                                                       >  1:1011519/1‑93 (MQ=255)

  gCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCa                                                                                       >  2:8055767/1‑93 (MQ=255)

    ttCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGc                                                                                     <  1:4948902/93‑1 (MQ=255)

     tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGTACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATGTTGCTCAGAGCTGTCAGCt                                                                                    >  2:9145292/1‑93 (MQ=255)

     tCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCt                                                                                    >  2:4045267/1‑93 (MQ=255)

      cATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTg                                                                                   >  2:9672183/1‑93 (MQ=255)

      cATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTg                                                                                   <  1:5948313/93‑1 (MQ=255)

       aTGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGc                                                                                  >  1:1468011/1‑93 (MQ=255)

       aTGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGc                                                                                  <  2:6106687/93‑1 (MQ=255)

        tGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGcc                                                                                 >  2:940614/1‑93 (MQ=255)

         ggTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCa                                                                                <  2:3912893/93‑1 (MQ=255)

          gTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAg                                                                               <  2:8563410/93‑1 (MQ=255)

            cAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGa                                                                             <  1:7520853/93‑1 (MQ=255)

              aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           >  1:209146/1‑93 (MQ=255)

              aGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATa                                                                           <  2:1273389/93‑1 (MQ=255)

               gACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATaa                                                                          >  1:2975616/1‑93 (MQ=255)

                   gTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCgg                                                                      <  1:2398227/93‑1 (MQ=255)

                    ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     >  2:3765132/1‑93 (MQ=255)

                    ttGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGt                                                                     <  1:4173685/93‑1 (MQ=255)

                     tGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGtt                                                                    <  2:8075902/93‑1 (MQ=255)

                      gAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGttt                                                                   >  2:2952536/1‑93 (MQ=255)

                      caaCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGttt                                                                   <  1:8365328/92‑1 (MQ=255)

                        aCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGa                                                                 >  2:3201063/1‑93 (MQ=255)

                        aCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGa                                                                 >  2:5800238/1‑93 (MQ=255)

                        aCTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGa                                                                 <  2:8724462/93‑1 (MQ=255)

                         cTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGAt                                                                >  2:8398411/1‑93 (MQ=255)

                         cTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGAt                                                                >  2:4553944/1‑93 (MQ=255)

                          tCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGGAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGCTTGATg                                                               >  2:6005046/1‑93 (MQ=255)

                          tCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATg                                                               >  1:5881238/1‑93 (MQ=255)

                          tCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATg                                                               <  1:3899527/93‑1 (MQ=255)

                           cGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGt                                                              <  1:880208/93‑1 (MQ=255)

                            gCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGtt                                                             <  1:7912265/93‑1 (MQ=255)

                             ccAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            <  2:7923548/93‑1 (MQ=255)

                             ccAGAGTTGAACAGCACGCCTTTTTCACTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTc                                                            <  2:6533491/93‑1 (MQ=255)

                              cAGAGTTGAACAGCACTTCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           <  1:9662766/93‑1 (MQ=255)

                              cAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           <  2:6971937/93‑1 (MQ=255)

                              cAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           >  2:8289228/1‑93 (MQ=255)

                              cAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           <  2:8533009/93‑1 (MQ=255)

                              cAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCa                                                           <  1:8124435/93‑1 (MQ=255)

                               agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          >  1:9830876/1‑93 (MQ=255)

                               agagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAg                                                          >  1:1256990/1‑93 (MQ=255)

                                gagTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAgg                                                         >  1:1117633/1‑93 (MQ=255)

                                   ttGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCg                                                      >  2:27620/1‑93 (MQ=255)

                                    tGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGt                                                     >  1:217697/1‑93 (MQ=255)

                                    tGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGt                                                     >  1:4591912/1‑93 (MQ=255)

                                    tGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGt                                                     <  1:6224300/93‑1 (MQ=255)

                                      aaCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGAAt                                                   >  2:5088817/1‑93 (MQ=255)

                                       aCAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGGTTGATGTTCAGGCCGTAtt                                                  >  1:3702767/1‑93 (MQ=255)

                                          gCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTag                                               <  1:9310771/93‑1 (MQ=255)

                                           cACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTaga                                              <  2:7167666/93‑1 (MQ=255)

                                           cACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTaga                                              <  1:3163400/93‑1 (MQ=255)

                                            aCGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTagag                                             >  2:5624212/1‑93 (MQ=255)

                                            aCGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTagag                                             >  2:8865892/1‑93 (MQ=255)

                                            aCGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCAAGGATAACCGGTTTGATGTTCAGGCCGTATTTagag                                             <  2:6759875/93‑1 (MQ=255)

                                             cGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATATCCGGTTTGATGTTCAGGCCGTATTTAGAgg                                            <  1:6810511/93‑1 (MQ=255)

                                              gCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGc                                           >  1:5168569/1‑93 (MQ=255)

                                              gCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGc                                           >  1:727343/1‑93 (MQ=255)

                                                cTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaa                                         <  2:2116710/93‑1 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  1:5207802/1‑93 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  1:7845538/1‑93 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  1:5269910/1‑93 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        <  2:3022481/93‑1 (MQ=255)

                                                 tttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCaaa                                        >  1:1378404/1‑93 (MQ=255)

                                                  ttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAAc                                       <  2:78062/93‑1 (MQ=255)

                                                  ttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAAc                                       <  2:1462759/93‑1 (MQ=255)

                                                  ttttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAAc                                       >  2:2206854/1‑93 (MQ=255)

                                                   tttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACt                                      >  2:6336569/1‑93 (MQ=255)

                                                    ttCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTc                                     <  1:4200665/93‑1 (MQ=255)

                                                     tcagtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCg                                    <  2:4692358/93‑1 (MQ=255)

                                                     tcagtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCg                                    <  1:5453209/93‑1 (MQ=255)

                                                      cagtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGTAAACTCGt                                   >  1:6918605/1‑93 (MQ=255)

                                                        gtcagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAg                                 <  1:660175/93‑1 (MQ=255)

                                                          cagGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTc                               <  1:2804077/93‑1 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              <  1:1846836/93‑1 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              >  2:1658134/1‑93 (MQ=255)

                                                           agGGCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcg                              <  1:348223/93‑1 (MQ=255)

                                                            gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             >  1:3790729/1‑93 (MQ=255)

                                                            gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             <  2:1852218/93‑1 (MQ=255)

                                                            gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             <  2:5840840/93‑1 (MQ=255)

                                                            gggCTTGCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTcgc                             >  2:3694247/1‑93 (MQ=255)

                                                                  tcTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGt                       <  1:2560295/92‑1 (MQ=255)

                                                                  gCTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGt                       <  1:6539363/93‑1 (MQ=255)

                                                                   cTGAGAAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTc                      >  1:7469760/1‑93 (MQ=255)

                                                                      agaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTg                   <  2:10045635/93‑1 (MQ=255)

                                                                       gaAAGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGc                  >  1:9414773/1‑93 (MQ=255)

                                                                         aaGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGccc                >  2:7430809/1‑93 (MQ=255)

                                                                         aaGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGccc                >  2:3421485/1‑93 (MQ=255)

                                                                          aGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGcccc               <  2:9000933/93‑1 (MQ=255)

                                                                          aGATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGcccc               <  2:9270849/93‑1 (MQ=255)

                                                                           gATCTGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCg              >  1:9534797/1‑93 (MQ=255)

                                                                               tGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTAc          <  2:7273774/93‑1 (MQ=255)

                                                                               tGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTAc          >  1:4297292/1‑93 (MQ=255)

                                                                               tGGCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTAc          <  1:470922/93‑1 (MQ=255)

                                                                                ggCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTAcc         <  2:547905/93‑1 (MQ=255)

                                                                                 gCTCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTActg        >  1:6464165/1‑91 (MQ=255)

                                                                                   tCAGAGCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGcc      >  1:7415019/1‑93 (MQ=255)

                                                                                       agCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATaa  <  2:5003103/93‑1 (MQ=255)

                                                                                       agCTGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTAGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATaa  <  1:1776018/93‑1 (MQ=255)

CCGCTTCATGGTCAAGACCGTTGAACTCGCCAGAGTTGAACAGCACGCCTTTTTCAGTCAGGGCTTGCTGAGAAAGATCTGGCTCAGAGCCGTCAGCTGCCAGGATAACCGGTTTGATGTTCAGGCCGTATTTAGAGGCAAACTCGTAGTCGCGCTGGTCGTGCCCCGGTACCGCCATAA  >  NC_000913/672824‑673003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`