BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,850	T→C	Q184R (CAG→CGG)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,850	0	T	C	90.7%	650.6 / 48.1	257	Q184R (CAG→CGG)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base T (12/12); new base C (107/126); total (119/138)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01

Only 100 of 271 total aligned reads displayed.

CCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  NC_000913/697758‑697942

ccTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCt                                                                                              <  2:3094084/93‑1 (MQ=255)

   gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGc                                                                                           >  2:6318067/1‑93 (MQ=255)

   gACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGc                                                                                           <  2:4406385/93‑1 (MQ=255)

      gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTcc                                                                                        >  1:3522376/1‑93 (MQ=255)

      gCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTcc                                                                                        <  1:4339268/93‑1 (MQ=255)

       cAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCca                                                                                       >  1:8544743/1‑93 (MQ=255)

        aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCcac                                                                                      >  2:527983/1‑93 (MQ=255)

        aGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCcac                                                                                      >  2:24790/1‑93 (MQ=255)

         gCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCcaca                                                                                     >  2:4581801/1‑93 (MQ=255)

          cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaa                                                                                    <  2:9526023/93‑1 (MQ=255)

          cTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaa                                                                                    <  1:9310741/93‑1 (MQ=255)

          cTCGTTTTTGTAGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACaa                                                                                    >  1:4766070/1‑93 (MQ=255)

           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                   <  2:9724999/93‑1 (MQ=255)

           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                   >  1:5802965/1‑93 (MQ=255)

           tCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACaaa                                                                                   <  2:378689/93‑1 (MQ=255)

            cGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAAt                                                                                  <  2:2556973/93‑1 (MQ=255)

            cGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAAt                                                                                  <  2:1244475/93‑1 (MQ=255)

             gTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATa                                                                                 >  2:4114675/1‑93 (MQ=255)

              tttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAg                                                                                >  2:4608420/1‑93 (MQ=255)

               ttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGc                                                                               >  1:4238103/1‑93 (MQ=255)

               ttttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGc                                                                               <  1:9798582/93‑1 (MQ=255)

                tttGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCt                                                                              >  2:3162349/1‑93 (MQ=255)

                 ttGTGGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTc                                                                             <  2:1104636/93‑1 (MQ=255)

                  tGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTcc                                                                            <  1:6082365/93‑1 (MQ=255)

                   gTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTccc                                                                           <  2:2684819/93‑1 (MQ=255)

                       gTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCccgcc                                                                       >  2:7934039/1‑93 (MQ=255)

                         cACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCgg                                                                     >  2:8779101/1‑93 (MQ=255)

                         cACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCgg                                                                     <  2:6185953/93‑1 (MQ=255)

                          aCGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCggg                                                                    <  1:1450031/93‑1 (MQ=255)

                             ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                                 >  2:9287578/1‑93 (MQ=255)

                             ttATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                                 >  2:531315/1‑93 (MQ=255)

                             ttATCTTTCACCGCATCGTAGGCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAc                                                                 >  1:1845614/1‑93 (MQ=255)

                              tATCTTTCCCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                                <  1:5772557/93‑1 (MQ=255)

                              tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                                >  2:9924578/1‑93 (MQ=255)

                              tATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAAct                                                                >  2:921943/1‑93 (MQ=255)

                               agttttGACCGCACCGGATTGGAACCAGTCTCGATGATAGTAAGAACGGATTTGGCAGTCCTGGGTCCACAAATAGCTCCCCGACGGGAActc                                                               <  1:7704287/90‑1 (MQ=255)

                                tCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAActct                                                              <  2:3584131/93‑1 (MQ=255)

                                   ttCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTa                                                           >  2:8102870/1‑93 (MQ=255)

                                    tCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa                                                          >  2:5890602/1‑93 (MQ=255)

                                    tCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTaa                                                          >  2:6377460/1‑93 (MQ=255)

                                     ccccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt                                                         <  1:2956981/91‑1 (MQ=255)

                                     cACCGCCTCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt                                                         <  1:2956977/93‑1 (MQ=255)

                                     cACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAAt                                                         <  1:2626424/93‑1 (MQ=255)

                                      cccGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                        <  1:1673768/92‑1 (MQ=255)

                                      aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                        <  1:6417230/93‑1 (MQ=255)

                                      aCCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATc                                                        <  2:1956156/93‑1 (MQ=255)

                                         gCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTg                                                     >  1:1012773/1‑93 (MQ=255)

                                         gCATCGTAGTCGAACCAGTCGAGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTg                                                     >  2:9815415/1‑93 (MQ=255)

                                          cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                    >  1:4823463/1‑93 (MQ=255)

                                          cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                    <  2:1767659/93‑1 (MQ=255)

                                          cATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGc                                                    <  2:816941/93‑1 (MQ=255)

                                            tCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCgg                                                  >  2:1508479/1‑93 (MQ=255)

                                            tCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCgg                                                  >  2:7362976/1‑93 (MQ=255)

                                             cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGTATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                                 <  1:5868673/93‑1 (MQ=255)

                                             cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                                 >  1:9108022/1‑93 (MQ=255)

                                             cGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGc                                                 <  2:3339268/93‑1 (MQ=255)

                                              gTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCa                                                >  1:2206313/1‑93 (MQ=255)

                                               tAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaa                                               >  2:9597233/1‑93 (MQ=255)

                                               tAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaa                                               >  1:6299820/1‑93 (MQ=255)

                                                aGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCaaa                                              <  2:4706324/93‑1 (MQ=255)

                                                 gTCGAACCGGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAc                                             >  1:1194918/1‑93 (MQ=255)

                                                  tCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAAcg                                            >  2:8731298/1‑92 (MQ=255)

                                                   cGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTg                                           <  1:3186030/93‑1 (MQ=255)

                                                    gAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTgg                                          >  1:2882265/1‑93 (MQ=255)

                                                    gAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTgc                                          >  1:3642022/1‑92 (MQ=255)

                                                     aaCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGc                                         <  1:218695/93‑1 (MQ=255)

                                                     aaCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGc                                         <  1:2751539/93‑1 (MQ=255)

                                                      aCCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAACCGTGGGGCAAACCGGcc                                        >  2:2111179/1‑92 (MQ=255)

                                                       ccAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCAc                                       >  2:8015250/1‑93 (MQ=255)

                                                         aGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCa                                     >  1:2465506/1‑93 (MQ=255)

                                                           tCGCGATGATAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAgc                                   <  2:49124/93‑1 (MQ=255)

                                                                atgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCttt                              >  1:8262307/1‑93 (MQ=255)

                                                                 tgatAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTc                             >  2:1814834/1‑93 (MQ=255)

                                                                 tgatAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTc                             <  1:8138732/93‑1 (MQ=255)

                                                                  gatAGGAAGAACGGAGTTCGCCGTCCCGGCTCCACAAAGAGCGCCCCGCCGGGAACTCTTCAATCGTGGGGCAAACTGGCACCAGCGCTTTCa                            <  1:4027257/93‑1 (MQ=255)

                                                                    tAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAtt                          <  2:9176488/93‑1 (MQ=255)

                                                                    tAGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAtt                          >  1:5457821/1‑93 (MQ=255)

                                                                     aGTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAttt                         <  1:4213066/93‑1 (MQ=255)

                                                                      gTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTc                        >  2:7601454/1‑93 (MQ=255)

                                                                      gTAAGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCAtttt                        >  1:4512924/1‑92 (MQ=255)

                                                                        aaGAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTg                      <  2:8658497/93‑1 (MQ=255)

                                                                         ataaCGAATGTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGa                     <  2:7075093/91‑1 (MQ=255)

                                                                         aGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGa                     >  1:2740092/1‑93 (MQ=255)

                                                                          gAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                    >  2:6973875/1‑93 (MQ=255)

                                                                          gAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                    >  1:7441461/1‑93 (MQ=255)

                                                                          gAACGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAg                    >  1:7206419/1‑93 (MQ=255)

                                                                            aCGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGc                  <  2:337256/93‑1 (MQ=255)

                                                                             cGGATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGcc                 >  1:9011762/1‑93 (MQ=255)

                                                                              ggATTTCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCca                <  1:7094016/93‑1 (MQ=255)

                                                                                   tCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAc           >  2:7171219/1‑93 (MQ=255)

                                                                                   tCGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATAc           <  1:7669455/93‑1 (MQ=255)

                                                                                    cGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACa          <  1:6647010/93‑1 (MQ=255)

                                                                                    cGCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACa          <  1:1651956/93‑1 (MQ=255)

                                                                                     gCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAg         <  2:8914915/93‑1 (MQ=255)

                                                                                     gCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAg         <  2:2427517/93‑1 (MQ=255)

                                                                                     gCCGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAg         >  1:3735458/1‑93 (MQ=255)

                                                                                       cGTCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCt       >  1:7956824/1‑93 (MQ=255)

                                                                                        gTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTg      >  1:8291986/1‑93 (MQ=255)

                                                                                        gTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTg      >  2:7518259/1‑93 (MQ=255)

                                                                                         tCCCGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGa     >  2:2553759/1‑93 (MQ=255)

CCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTTCGCCGTCCTGGCTCCACAAATAGCTCCCCGCCGGGAACTCTTTAATCGTGCGGCAAACTGGCACCAGCGCTTTCATTTCTGAGGCCACATACAGCTGACCG  >  NC_000913/697758‑697942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`