BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,954	G→A	G83G (GGC→GGT)	kdpC ←	KdpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,954	0	G	A	99.2%	917.8 / ‑5.6	263	G83G (GGC→GGT)	kdpC	KdpC
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (153/108); total (153/110)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 276 total aligned reads displayed.

CTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAA  >  NC_000913/723865‑724043

cTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCccacca                                                                                        <  1:146497/93‑1 (MQ=255)

  ggCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGa                                                                                      >  2:6176856/1‑93 (MQ=255)

   gCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGaa                                                                                     <  2:4174909/93‑1 (MQ=255)

    cATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAg                                                                                    >  2:9254832/1‑93 (MQ=255)

     aTCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGc                                                                                   >  1:8260999/1‑93 (MQ=255)

      tCCGGGTTCGCGGCCAGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCACGTCAGGGTTAATGACCGCCAGATTGGTCCCGCCAGAAGcc                                                                                  >  2:2430095/1‑93 (MQ=255)

      tCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGcc                                                                                  >  2:85192/1‑93 (MQ=255)

      tCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGcc                                                                                  >  1:5292972/1‑93 (MQ=255)

        cGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTTTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCAACAGAAGCCtt                                                                                >  2:6610213/1‑92 (MQ=255)

        cGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtg                                                                                >  1:522502/1‑93 (MQ=255)

        cGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtg                                                                                <  1:2357563/93‑1 (MQ=255)

          ggTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtgtg                                                                              <  1:6039600/93‑1 (MQ=255)

          ggTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtgtg                                                                              >  1:2835509/1‑93 (MQ=255)

           gTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTgg                                                                             <  2:36276/93‑1 (MQ=255)

            ttAGGGGCCCGTAATGCGGCAACGCGTGTGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCGAGATTGCTCCCACCAGAAGCCTGTGGa                                                                            <  1:6787908/93‑1 (MQ=255)

            ttAGCGGCACGTAATGCAGGAACGCGTGCGGCTATTAGTTTAGCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGa                                                                            <  1:1103424/93‑1 (MQ=255)

             tAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGAt                                                                           >  1:4423831/1‑93 (MQ=255)

                cGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACAAGAAGCCTGTGGttttt                                                                        >  2:7475378/1‑91 (MQ=255)

                  gCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAg                                                                      >  2:5479796/1‑93 (MQ=255)

                    ccGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAggg                                                                    <  1:702281/93‑1 (MQ=255)

                    ccGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGATCCCACCAGAAGCCTGTGGATTATAggg                                                                    >  1:2073957/1‑93 (MQ=255)

                       tAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAt                                                                 >  1:4710176/1‑93 (MQ=255)

                       tAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAt                                                                 >  2:262042/1‑93 (MQ=255)

                       tAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAt                                                                 <  1:5600143/93‑1 (MQ=255)

                         aTGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAttt                                                               <  2:2003320/93‑1 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              >  1:8279959/1‑93 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              <  2:1876357/93‑1 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              >  2:1958750/1‑93 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              >  1:7863866/1‑93 (MQ=255)

                            cagcaACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTg                                                            >  1:1920342/1‑93 (MQ=255)

                              gcaACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGcc                                                          >  1:4259525/1‑93 (MQ=255)

                              gcaACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGcc                                                          >  1:8062832/1‑93 (MQ=255)

                               caACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCg                                                         <  1:9475001/93‑1 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        <  2:559891/93‑1 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        >  1:78671/1‑93 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        <  2:2297478/93‑1 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        <  2:6337437/93‑1 (MQ=255)

                                 aCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGtt                                                       >  1:1866393/1‑93 (MQ=255)

                                   gcgTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGc                                                     >  2:5136078/1‑93 (MQ=255)

                                   gcgTGCGGCGATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGc                                                     <  2:5031546/93‑1 (MQ=255)

                                    cgTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGcc                                                    >  1:9045891/1‑93 (MQ=255)

                                     gTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTTCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGcag                                                   >  1:6447228/1‑91 (MQ=255)

                                     gTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCg                                                   <  2:832129/93‑1 (MQ=255)

                                      tGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTGTAGGGCATTTCTGCCGTTGCCGa                                                  >  2:6939706/1‑93 (MQ=255)

                                      tGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGa                                                  <  1:1609059/93‑1 (MQ=255)

                                        cGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACg                                                >  1:6020581/1‑93 (MQ=255)

                                        cGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACg                                                <  1:310171/93‑1 (MQ=255)

                                         ggCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACgg                                               >  2:1302224/1‑93 (MQ=255)

                                          gCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACggg                                              >  1:100808/1‑93 (MQ=255)

                                           cTATTAGTTTATCCGGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGc                                             <  1:6340291/93‑1 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  1:5135168/1‑93 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  1:3851771/1‑93 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  1:7974798/1‑93 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  2:3247031/1‑93 (MQ=255)

                                              ttAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAc                                          >  1:1211473/1‑93 (MQ=255)

                                              ttAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAc                                          >  2:2080612/1‑93 (MQ=255)

                                               tAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAcc                                         >  2:5402273/1‑93 (MQ=255)

                                                 gTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCAt                                       <  1:8574118/93‑1 (MQ=255)

                                                  tttATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCGTTTCTGCCGTTGCCGACGGGCGACCATg                                      >  2:9004873/1‑93 (MQ=255)

                                                    tATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaa                                    >  2:3433161/1‑93 (MQ=255)

                                                     aTCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaa                                   <  2:8706778/93‑1 (MQ=255)

                                                     aTCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaa                                   <  2:7035983/93‑1 (MQ=255)

                                                      tCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaaa                                  >  2:7157559/1‑93 (MQ=255)

                                                      tCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaaa                                  <  1:3659937/93‑1 (MQ=255)

                                                       ccAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAAt                                 <  1:1316984/93‑1 (MQ=255)

                                                        catcTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATa                                <  1:9102656/90‑1 (MQ=255)

                                                        cAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATa                                <  1:5877134/93‑1 (MQ=255)

                                                        cAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATa                                <  1:3407515/93‑1 (MQ=255)

                                                         aGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAg                               >  2:3971738/1‑93 (MQ=255)

                                                         aGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAg                               >  1:2564410/1‑93 (MQ=255)

                                                         aGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAg                               >  2:1739580/1‑93 (MQ=255)

                                                          gCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGc                              >  2:120348/1‑93 (MQ=255)

                                                          gCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGc                              <  2:9567029/93‑1 (MQ=255)

                                                            tCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCg                            <  2:4854154/93‑1 (MQ=255)

                                                            tCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCg                            >  1:3532459/1‑93 (MQ=255)

                                                            tCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCg                            >  2:2901434/1‑93 (MQ=255)

                                                             cAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGt                           <  2:599358/93‑1 (MQ=255)

                                                                ggTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGc                        >  2:3787049/1‑93 (MQ=255)

                                                                   tACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCgg                     <  1:1560053/93‑1 (MQ=255)

                                                                   tACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCgg                     >  1:1697046/1‑93 (MQ=255)

                                                                    aCTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGt                    >  2:1552338/1‑93 (MQ=255)

                                                                      tGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTaa                  >  1:7483921/1‑93 (MQ=255)

                                                                        aCCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTaaaa                <  2:8474904/93‑1 (MQ=255)

                                                                        aCCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACAATGAAAATAGCCGTTGCCGGTaaaa                <  2:8831948/93‑1 (MQ=255)

                                                                          cGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAAtt              <  2:4320886/93‑1 (MQ=255)

                                                                           gCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTc             >  1:4931442/1‑93 (MQ=255)

                                                                           gCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTc             >  1:88917/1‑93 (MQ=255)

                                                                            ccAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCt            >  1:7693103/1‑93 (MQ=255)

                                                                             cAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTg           >  2:9619125/1‑93 (MQ=255)

                                                                              aGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGc          <  2:8159554/93‑1 (MQ=255)

                                                                              aGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGc          >  2:4973325/1‑93 (MQ=255)

                                                                              aGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGc          <  1:9139658/93‑1 (MQ=255)

                                                                               gATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGcc         >  2:7848170/1‑93 (MQ=255)

                                                                               gATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGcc         >  2:8071410/1‑93 (MQ=255)

                                                                                aTTGCCCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATACCCGTTGCCGGTAAAATTCTGccc        <  2:4493918/93‑1 (MQ=255)

                                                                                 ttGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCg       >  2:6486324/1‑93 (MQ=255)

                                                                                    cTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGAtt    >  1:2304439/1‑93 (MQ=255)

                                                                                     tCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTa   >  2:5314014/1‑93 (MQ=255)

                                                                                      cccACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTaa  <  2:7396180/93‑1 (MQ=255)

                                                                                      cccACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTCGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTaa  <  2:9854670/93‑1 (MQ=255)

CTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAA  >  NC_000913/723865‑724043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`