BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	735,021	C→T	A50V (GCC→GTC)	ybfC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	735,021	0	C	T	99.8%	1907.1 / ‑5.8	528	A50V (GCC→GTC)	ybfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (255/271); total (257/271)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 535 total aligned reads displayed.

ATTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGCCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAA  >  NC_000913/734931‑735110

aTTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCa                                                                                         >  1:1447415/1‑93 (MQ=255)

aTTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCa                                                                                         <  2:9158700/93‑1 (MQ=255)

 ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAt                                                                                        <  2:5824583/93‑1 (MQ=255)

 ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAt                                                                                        >  2:2858795/1‑93 (MQ=255)

 ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAt                                                                                        <  2:7630534/93‑1 (MQ=255)

 ttGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAt                                                                                        >  2:4786536/1‑93 (MQ=255)

  tGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCAtg                                                                                       <  2:9088466/93‑1 (MQ=255)

      aaaTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGga                                                                                   >  1:2533755/1‑93 (MQ=255)

      aaaTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGga                                                                                   <  2:2600633/93‑1 (MQ=255)

        aTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGgaga                                                                                 >  1:3782955/1‑93 (MQ=255)

        aTACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGgaga                                                                                 >  1:7196655/1‑93 (MQ=255)

         tACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGAt                                                                                >  2:3046945/1‑93 (MQ=255)

           cAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATgg                                                                              >  2:1267206/1‑93 (MQ=255)

           cAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATgg                                                                              >  1:2714279/1‑93 (MQ=255)

           cAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATgg                                                                              <  2:10041522/93‑1 (MQ=255)

              gCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTATTAAATAATAAAGTCATGTGGAGATGGTAt                                                                           <  1:4470392/93‑1 (MQ=255)

               cTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATc                                                                          >  2:7830923/1‑93 (MQ=255)

                tAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCa                                                                         >  2:4688806/1‑93 (MQ=255)

                tAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCa                                                                         >  2:6311130/1‑93 (MQ=255)

                  aaGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGa                                                                       <  1:1568845/93‑1 (MQ=255)

                   aGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGaa                                                                      <  1:174585/93‑1 (MQ=255)

                    gATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAAt                                                                     <  2:3509266/93‑1 (MQ=255)

                      tCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGa                                                                   <  2:2679485/93‑1 (MQ=255)

                        aTGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAgg                                                                 >  1:9601607/1‑93 (MQ=255)

                        aTGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAgg                                                                 >  1:6924483/1‑93 (MQ=255)

                         tGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGa                                                                <  1:4110497/93‑1 (MQ=255)

                         tGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGa                                                                <  2:4074032/93‑1 (MQ=255)

                         tGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGa                                                                >  1:577029/1‑93 (MQ=255)

                          gATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGAc                                                               >  1:8589059/1‑93 (MQ=255)

                           aTTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACa                                                              <  1:8411157/93‑1 (MQ=255)

                            ttGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACAt                                                             >  2:3443630/1‑93 (MQ=255)

                            ttGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACAt                                                             >  2:3957203/1‑93 (MQ=255)

                              gTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGGGGAGATGGGATCAGAATGAGAACATCg                                                           >  1:7772463/1‑93 (MQ=255)

                               ttttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCgg                                                          >  2:2345701/1‑93 (MQ=255)

                               ttttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCgg                                                          >  2:8320083/1‑93 (MQ=255)

                               ttttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCgg                                                          >  1:4839414/1‑93 (MQ=255)

                               ttttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTGCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCgg                                                          <  2:9436593/93‑1 (MQ=255)

                                tttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGt                                                         >  2:8770260/1‑93 (MQ=255)

                                tttACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGt                                                         <  2:430157/93‑1 (MQ=255)

                                  tACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGATATCGGTGa                                                       <  2:7984005/93‑1 (MQ=255)

                                  tACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGa                                                       <  1:8794337/93‑1 (MQ=255)

                                  tACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGGATCAGAATGAGGACATCGGTGa                                                       >  2:6761428/1‑93 (MQ=255)

                                   aCTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAg                                                      >  1:7249573/1‑93 (MQ=255)

                                      aTTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtat                                                   <  1:4247235/93‑1 (MQ=255)

                                       ttttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtatt                                                  <  2:64171/93‑1 (MQ=255)

                                        tttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtatta                                                 <  1:333286/93‑1 (MQ=255)

                                         ttCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGtattat                                                <  1:5266573/93‑1 (MQ=255)

                                          tCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTattatt                                               <  1:792208/93‑1 (MQ=255)

                                          tCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTattatt                                               >  2:3851418/1‑93 (MQ=255)

                                           cGCTAAGTGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTg                                              <  2:6963981/93‑1 (MQ=255)

                                             cTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGc                                            >  1:9261149/1‑93 (MQ=255)

                                               aaGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCaa                                          >  1:5917423/1‑93 (MQ=255)

                                                aGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCaaa                                         <  1:9754912/93‑1 (MQ=255)

                                                aGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCaaa                                         >  2:8952708/1‑93 (MQ=255)

                                                  ggTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACa                                       <  2:1483897/93‑1 (MQ=255)

                                                  ggTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACa                                       <  2:4886110/93‑1 (MQ=255)

                                                  ggTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACa                                       >  1:4994050/1‑93 (MQ=255)

                                                    tACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGa                                     >  2:6060677/1‑93 (MQ=255)

                                                     aCTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGaa                                    <  2:2086739/93‑1 (MQ=255)

                                                     aCTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGaa                                    <  1:10004523/93‑1 (MQ=255)

                                                     aCTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGaa                                    <  1:13404/93‑1 (MQ=255)

                                                      cTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAAc                                   <  1:2991702/93‑1 (MQ=255)

                                                       tATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACt                                  >  2:6168/1‑93 (MQ=255)

                                                         ttttttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTgg                                >  1:8896986/1‑93 (MQ=255)

                                                          tttttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTggg                               >  1:6600375/1‑93 (MQ=255)

                                                            tttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTa                             <  2:1825130/93‑1 (MQ=255)

                                                            tttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTa                             <  1:682435/93‑1 (MQ=255)

                                                             ttCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTAc                            <  1:7526437/93‑1 (MQ=255)

                                                              tCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACa                           <  1:4766088/93‑1 (MQ=255)

                                                              tCCGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACa                           >  1:6176565/1‑93 (MQ=255)

                                                               ccGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACAt                          <  2:9809991/93‑1 (MQ=255)

                                                               ccGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACAt                          >  1:5599730/1‑93 (MQ=255)

                                                               ccGCGTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACAt                          <  2:5964799/93‑1 (MQ=255)

                                                                cgcgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATg                         >  1:1153803/1‑93 (MQ=255)

                                                                cgcgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATg                         <  1:210676/93‑1 (MQ=255)

                                                                 gcgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGt                        <  2:5006964/93‑1 (MQ=255)

                                                                 gcgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGt                        <  2:1343537/93‑1 (MQ=255)

                                                                 gcgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGt                        >  1:9331897/1‑93 (MQ=255)

                                                                  cgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTa                       <  2:8617600/93‑1 (MQ=255)

                                                                  cgTTTCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTa                       <  2:7746607/93‑1 (MQ=255)

                                                                      tCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTaaaaa                   >  2:5610027/1‑93 (MQ=255)

                                                                      tCCTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTaaaaa                   >  1:9209960/1‑93 (MQ=255)

                                                                       ccTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaat                  <  1:2862504/93‑1 (MQ=255)

                                                                        cTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaata                 >  2:5354194/1‑93 (MQ=255)

                                                                        cTGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaata                 >  2:806726/1‑93 (MQ=255)

                                                                         tGTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaataa                <  1:3170901/93‑1 (MQ=255)

                                                                          gTCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAaataat               >  1:3963986/1‑93 (MQ=255)

                                                                           tCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAataata              <  1:8268146/93‑1 (MQ=255)

                                                                           tCTTAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAataata              >  1:4510064/1‑93 (MQ=255)

                                                                             ttAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATaaa            >  2:6652054/1‑93 (MQ=255)

                                                                             ttAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATaaa            >  2:1732691/1‑93 (MQ=255)

                                                                             ttAAATAATAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATaaa            <  2:9616902/93‑1 (MQ=255)

                                                                                aataatAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTc         <  2:3252115/93‑1 (MQ=255)

                                                                                aataatAAAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTc         <  1:2401778/93‑1 (MQ=255)

                                                                                   aataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTcaca      >  2:7367246/1‑93 (MQ=255)

                                                                                    ataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCacac     <  1:5434855/93‑1 (MQ=255)

                                                                                    ataaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCacac     >  2:4445980/1‑93 (MQ=255)

                                                                                     taaAGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACAcc    <  2:7535726/93‑1 (MQ=255)

                                                                                       aaGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCaa  <  1:8013312/93‑1 (MQ=255)

                                                                                       aaGTCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCaa  <  2:841810/93‑1 (MQ=255)

ATTGCGAAATACAAGCTAAAGATCATGATTGTTTTACTATTTTCGCTAAGGGTACTATTTTTTCCGCGTTTCCTGTCTTAAATAATAAAGCCATGTGGAGATGGTATCAGAATGAGGACATCGGTGAGTATTATTGGCAAACAGAACTGGGTACATGTAAAAATAATAAATTCACACCAA  >  NC_000913/734931‑735110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`