BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	775,372	G→A	A101T (GCC→ACC)	tolR →	TolF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	775,372	0	G	A	99.4%	1173.6 / ‑5.8	329	A101T (GCC→ACC)	tolR	TolF
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (189/138); total (189/140)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

Only 100 of 343 total aligned reads displayed.

ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG  >  NC_000913/775282‑775461

aTTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAcc                                                                                         <  1:4096257/93‑1 (MQ=255)

aTTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAcc                                                                                         >  1:525017/1‑93 (MQ=255)

 ttGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCa                                                                                        >  1:4864958/1‑93 (MQ=255)

  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTGCCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       <  2:8032240/93‑1 (MQ=255)

  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       >  2:20100/1‑93 (MQ=255)

  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       <  1:3077264/93‑1 (MQ=255)

     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                    >  1:8445616/1‑93 (MQ=255)

       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGGTTCAAGACCAACCCGa                                                                                  >  2:154440/1‑93 (MQ=255)

       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTAGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGa                                                                                  >  2:5926785/1‑93 (MQ=255)

        gTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaa                                                                                 >  2:2206611/1‑93 (MQ=255)

          acacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaaaa                                                                               <  1:9541051/93‑1 (MQ=255)

           cacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAc                                                                              >  1:4531477/1‑93 (MQ=255)

             ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACgg                                                                            <  2:2054495/93‑1 (MQ=255)

               gtggtgGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTc                                                                          >  2:7104655/1‑93 (MQ=255)

                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                         >  1:8050281/1‑93 (MQ=255)

                 ggtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCtt                                                                        <  1:1952566/93‑1 (MQ=255)

                  gtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCttt                                                                       >  1:3882025/1‑93 (MQ=255)

                   tggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTc                                                                      >  1:5031945/1‑93 (MQ=255)

                    ggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCt                                                                     <  1:2923983/93‑1 (MQ=255)

                    ggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCt                                                                     >  1:5909535/1‑93 (MQ=255)

                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  1:1335988/93‑1 (MQ=255)

                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  2:1726835/93‑1 (MQ=255)

                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   >  2:4478059/1‑93 (MQ=255)

                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACAAACCCGAAAACGGTCTTTCTgt                                                                   >  2:6905719/1‑92 (MQ=255)

                      gttagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  2:7696254/90‑1 (MQ=255)

                       tGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAt                                                                  >  1:3569467/1‑93 (MQ=255)

                       tGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAt                                                                  >  2:4019024/1‑93 (MQ=255)

                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGagc                                                                 <  2:5646028/93‑3 (MQ=255)

                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 >  1:4653045/1‑93 (MQ=255)

                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 <  1:7568664/93‑1 (MQ=255)

                         agaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCg                                                                >  2:4807249/1‑93 (MQ=255)

                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                               <  2:4328379/93‑1 (MQ=255)

                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                               <  1:4735635/93‑1 (MQ=255)

                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                               >  2:9247436/1‑93 (MQ=255)

                           aaaGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGt                                                              >  1:128314/1‑93 (MQ=255)

                             aGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                            <  2:3305506/93‑1 (MQ=255)

                             aGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                            >  2:1753550/1‑93 (MQ=255)

                                 cGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCa                                                        <  1:4392992/93‑1 (MQ=255)

                                 cGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGCCTTTCTGATCGGTGGCGCa                                                        >  2:8182362/1‑93 (MQ=255)

                                  gCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaa                                                       >  2:8854829/1‑93 (MQ=255)

                                   ccTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaa                                                      >  2:937888/1‑93 (MQ=255)

                                    cTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaaa                                                     >  1:8274821/1‑93 (MQ=255)

                                    cTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaaa                                                     >  2:365964/1‑93 (MQ=255)

                                     tGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAg                                                    >  1:2497580/1‑93 (MQ=255)

                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                   <  2:3422741/93‑1 (MQ=255)

                                       gAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                                  >  1:6726748/1‑93 (MQ=255)

                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 <  2:9631640/93‑1 (MQ=255)

                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 >  1:279926/1‑93 (MQ=255)

                                          cGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtgtg                                               >  2:3535427/1‑93 (MQ=255)

                                            tttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGcc                                             >  2:1328610/1‑93 (MQ=255)

                                              tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCtt                                           >  2:4902530/1‑93 (MQ=255)

                                              tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCtt                                           <  1:6942878/93‑1 (MQ=255)

                                              tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCtt                                           >  1:4896000/1‑93 (MQ=255)

                                                ccaccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                         <  1:9249604/93‑1 (MQ=255)

                                                ccaccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                         <  2:8627948/93‑1 (MQ=255)

                                                 caccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACg                                        >  2:4597106/1‑93 (MQ=255)

                                                 caccaGAGCAGGAGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACg                                        >  2:3067881/1‑93 (MQ=255)

                                                   ccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                      <  2:8087882/93‑1 (MQ=255)

                                                    caGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATg                                     >  2:1201738/1‑93 (MQ=255)

                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  1:2116395/1‑93 (MQ=255)

                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  2:613679/1‑93 (MQ=255)

                                                      gagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                   >  1:3791757/1‑93 (MQ=255)

                                                      gagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                   >  1:7115775/1‑93 (MQ=255)

                                                         cAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaata                                <  1:9398333/93‑1 (MQ=255)

                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                              >  2:2398879/1‑93 (MQ=255)

                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                              >  1:3301491/1‑93 (MQ=255)

                                                            gtggtgGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAtt                             >  1:2188954/1‑93 (MQ=255)

                                                              ggtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaa                           <  1:5457315/93‑1 (MQ=255)

                                                              ggtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaa                           <  2:5799815/93‑1 (MQ=255)

                                                               gtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaaa                          <  2:4255423/93‑1 (MQ=255)

                                                                tggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAg                         <  2:7777732/93‑1 (MQ=255)

                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGc                        <  1:9845031/93‑1 (MQ=255)

                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGc                        <  2:4681646/93‑1 (MQ=255)

                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTGACGATGAAATAATTAAAga                        >  2:634230/1‑92 (MQ=255)

                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:9743688/93‑1 (MQ=255)

                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       >  1:7292755/1‑93 (MQ=255)

                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:6321631/93‑1 (MQ=255)

                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:5769364/93‑1 (MQ=255)

                                                                     gAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                    >  2:2557270/1‑93 (MQ=255)

                                                                      aaGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGa                   >  1:9137695/1‑93 (MQ=255)

                                                                       aGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGaa                  <  1:6047307/93‑1 (MQ=255)

                                                                       aGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGaa                  >  1:7840037/1‑93 (MQ=255)

                                                                        gtgtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAAc                 <  2:2784493/93‑1 (MQ=255)

                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              >  2:1614060/1‑93 (MQ=255)

                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              >  1:9186809/1‑93 (MQ=255)

                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCGTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              <  1:9670276/93‑1 (MQ=255)

                                                                             cAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGtt            <  2:5187536/93‑1 (MQ=255)

                                                                              aGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTa           <  1:3643198/93‑1 (MQ=255)

                                                                               gCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTAc          <  1:5644649/93‑1 (MQ=255)

                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa         >  2:6910094/1‑93 (MQ=255)

                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa         <  2:5482822/93‑1 (MQ=255)

                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt        <  1:6583498/93‑1 (MQ=255)

                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  2:1909503/1‑93 (MQ=255)

                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  1:1654083/1‑93 (MQ=255)

                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  2:3784549/1‑93 (MQ=255)

                                                                                    ttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGt     <  1:8822456/93‑1 (MQ=255)

                                                                                    ttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGt     <  1:3981305/93‑1 (MQ=255)

                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    <  2:9152282/93‑1 (MQ=255)

                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    >  2:66395/1‑93 (MQ=255)

                                                                                       aaGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCg  >  2:7506173/1‑93 (MQ=255)

ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG  >  NC_000913/775282‑775461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`