Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 79,855 A→G V337A (GTG→GCG)  leuC ← 3‑isopropylmalate isomerase (dehydratase) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091379,8550AG99.6% 893.4 / ‑6.4 280V337A (GTG→GCG) leuC3‑isopropylmalate isomerase (dehydratase) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (149/130);  minor base C (0/1);  total (149/131)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

ACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCC  >  NC_000913/79765‑79944
                                                                                          |                                                                                         
aCTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCt                                                                                         >  2:1958718/1‑93 (MQ=255)
aCTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCt                                                                                         >  1:2893694/1‑93 (MQ=255)
 cTTTTCGCCCTTTGGCGAGCTCCGCTGCCGCGCGTAAATCTGCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        <  2:2575380/93‑1 (MQ=255)
  ttttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTc                                                                                       >  2:6079863/1‑93 (MQ=255)
  ttttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTc                                                                                       >  2:5870933/1‑93 (MQ=255)
   tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCg                                                                                      <  1:7932017/93‑1 (MQ=255)
    ttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCgg                                                                                     <  1:2217840/93‑1 (MQ=255)
        cccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAg                                                                                 <  2:2361666/93‑1 (MQ=255)
        cccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAg                                                                                 <  2:8050033/93‑1 (MQ=255)
        cccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAg                                                                                 >  1:6225215/1‑93 (MQ=255)
         ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGc                                                                                <  2:2528600/93‑1 (MQ=255)
          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               >  1:9003413/1‑93 (MQ=255)
           tttGGCGATCTCCGCTGCTGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGTACCGCTAAACACTTTGTCGATAGCCGCTTCGGTCAGCgg                                                                              >  1:6742354/1‑93 (MQ=255)
           tttGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCgg                                                                              <  1:4774432/93‑1 (MQ=255)
            ttGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGa                                                                             >  1:4954900/1‑93 (MQ=255)
              ggCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAAt                                                                           <  1:1925587/93‑1 (MQ=255)
              ggCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCAGCTTCGGTCAGCGGAAt                                                                           <  2:1375895/93‑1 (MQ=255)
                cGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAc                                                                         <  1:6086706/93‑1 (MQ=255)
                 gATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAcc                                                                        <  1:984087/93‑1 (MQ=255)
                 gATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAcc                                                                        <  1:5301017/93‑1 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  2:499263/1‑93 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  1:10041387/1‑93 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  2:4871750/1‑93 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  1:3513499/1‑93 (MQ=255)
                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  2:8237555/1‑93 (MQ=255)
                   tctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCg                                                                      >  2:9355873/1‑93 (MQ=255)
                    ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCgg                                                                     >  1:3145064/1‑93 (MQ=255)
                    ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGGCAGCGGAATACCCgt                                                                     >  1:7651050/1‑92 (MQ=255)
                     tcCGCTGCCGCGCGTAAATCTTCAATGCGCGCGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTTTGCGGAATATCCGGt                                                                    >  2:7320543/1‑93 (MQ=255)
                      ccGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGTCGCTTCGGTCCGCGGAATACCCGGtt                                                                   >  2:8330889/1‑93 (MQ=255)
                      ccGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGtt                                                                   >  2:6651564/1‑93 (MQ=255)
                       cGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGttt                                                                  <  2:1517313/93‑1 (MQ=255)
                       cGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGttt                                                                  >  2:3696097/1‑93 (MQ=255)
                        gCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTc                                                                 >  2:8623621/1‑93 (MQ=255)
                         cTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCa                                                                <  1:411547/93‑1 (MQ=255)
                         cTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCa                                                                >  2:4017600/1‑93 (MQ=255)
                          tGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGAGGAATACCCGGTTTCAg                                                               >  2:2766576/1‑93 (MQ=255)
                           gCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGc                                                              >  2:5623199/1‑93 (MQ=255)
                             cgcgcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGccc                                                            >  2:9620843/1‑93 (MQ=255)
                                gcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCat                                                         <  2:4154655/93‑1 (MQ=255)
                                gcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCat                                                         >  2:7924456/1‑93 (MQ=255)
                                 cgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata                                                        <  2:3567609/93‑1 (MQ=255)
                                  gTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCatat                                                       >  2:2936308/1‑93 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      >  1:5436458/1‑93 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      >  2:1800862/1‑93 (MQ=255)
                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      >  1:8924932/1‑93 (MQ=255)
                                    aaaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg                                                     <  2:4905308/93‑1 (MQ=255)
                                      aTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGc                                                   <  2:9754106/93‑1 (MQ=255)
                                        cTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCa                                                 >  1:4752285/1‑93 (MQ=255)
                                        cTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCa                                                 <  1:7063968/93‑1 (MQ=255)
                                             aTGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCt                                            <  2:9856148/93‑1 (MQ=255)
                                              tGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtt                                           >  2:3546792/1‑93 (MQ=255)
                                               gcgcGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCttt                                          <  1:6256224/93‑1 (MQ=255)
                                               gcgcGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGAGGAATACACGGTTTCAGCCCCATATAGGCCAGCGCttt                                          >  1:5974214/1‑93 (MQ=255)
                                                 gcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCttttt                                        >  2:9021389/1‑93 (MQ=255)
                                                 gcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCttttt                                        >  2:3269191/1‑93 (MQ=255)
                                                  cgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATCCCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTc                                       <  2:7090697/93‑1 (MQ=255)
                                                  cgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTc                                       <  1:380738/93‑1 (MQ=255)
                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      <  2:7381944/93‑1 (MQ=255)
                                                    aGTTTGTACACGACCCGATAAACCCTTCGTCGATCGCCGCTCCGGTCAGCGAAATACCAGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTg                                     <  2:6044152/93‑1 (MQ=255)
                                                      ttGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGcc                                   >  1:1574982/1‑93 (MQ=255)
                                                        ggTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGa                                 >  1:1149345/1‑93 (MQ=255)
                                                         gTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAc                                <  1:7284420/93‑1 (MQ=255)
                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTTGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              >  1:9267113/1‑93 (MQ=255)
                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:5600826/93‑1 (MQ=255)
                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:2045958/93‑1 (MQ=255)
                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:4450356/93‑1 (MQ=255)
                                                             aGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgcgc                            >  1:7539392/1‑93 (MQ=255)
                                                             aGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgcgc                            >  1:3933405/1‑93 (MQ=255)
                                                               gAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGt                          <  1:1473875/93‑1 (MQ=255)
                                                                aaCCGATAAACACTTTGTCGAAAGCCGCTTCGGTCAGCGGAATACCCGGCTTCAGCCCCATATAGGCCAGCGCTTCTTCTGCCGAcgcgcgac                         >  2:7695925/1‑91 (MQ=255)
                                                                 aCCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTc                        <  1:3350561/93‑1 (MQ=255)
                                                                 aCCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTc                        <  1:4613244/93‑1 (MQ=255)
                                                                 aCCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTc                        <  1:2684732/93‑1 (MQ=255)
                                                                  ccGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCa                       <  2:2325215/93‑1 (MQ=255)
                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCaa                      <  2:4394860/93‑1 (MQ=255)
                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCaa                      <  1:6339804/93‑1 (MQ=255)
                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     <  1:9420716/93‑1 (MQ=255)
                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACCCGCGTTCAAc                     >  1:8243964/1‑93 (MQ=255)
                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCCAcc                    >  2:1138741/1‑93 (MQ=255)
                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCCACGCGCGTTCAAcc                    >  2:4581962/1‑93 (MQ=255)
                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCg                   >  2:8431045/1‑93 (MQ=255)
                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCg                   >  1:8393512/1‑93 (MQ=255)
                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCACAGCTATTTCTGCCGACGCGAGTTAAACCg                   >  1:99730/1‑93 (MQ=255)
                                                                       aaaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCgg                  >  2:2276979/1‑93 (MQ=255)
                                                                       aaaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCgg                  >  1:989393/1‑93 (MQ=255)
                                                                        aaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCACCCGGa                 >  1:3302084/1‑93 (MQ=255)
                                                                         acacTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGAt                >  2:6364355/1‑93 (MQ=255)
                                                                         acacTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGAt                <  1:5060722/93‑1 (MQ=255)
                                                                          cacTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATc               <  2:8770130/93‑1 (MQ=255)
                                                                           ccTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCg              <  2:514079/92‑1 (MQ=255)
                                                                           acTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCg              <  1:8548032/93‑1 (MQ=255)
                                                                             tttGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGc            >  1:2421409/1‑93 (MQ=255)
                                                                                ttCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         <  1:9557227/92‑1 (MQ=255)
                                                                                gTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGCCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         <  1:8914443/93‑1 (MQ=255)
                                                                                   gATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      <  1:8278701/93‑1 (MQ=255)
                                                                                   gATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      <  2:5580665/93‑1 (MQ=255)
                                                                                    aTAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGaa     >  2:68786/1‑93 (MQ=255)
                                                                                    aTAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGaa     >  1:867243/1‑93 (MQ=255)
                                                                                       gCCGCTGCGGTCAGCGGAATCCCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  <  2:5379469/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCC  >  NC_000913/79765‑79944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑