BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,040	A→G	N399S (AAC→AGC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,040	0	A	G	99.2%	789.3 / ‑3.5	247	N399S (AAC→AGC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (109/136); total (110/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 256 total aligned reads displayed.

TCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT  >  NC_000913/990950‑991129

tCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCt                                                                                         >  2:1639315/1‑93 (MQ=255)

tCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCt                                                                                         >  1:7588477/1‑93 (MQ=255)

 cATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCtt                                                                                        <  1:1455567/93‑1 (MQ=255)

  aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       >  2:2400190/1‑93 (MQ=255)

  aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       <  2:9511856/93‑1 (MQ=255)

   ttGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTcc                                                                                      <  2:6992711/93‑1 (MQ=255)

    tGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCa                                                                                     <  2:7240995/93‑1 (MQ=255)

     gagaTGAACAACTTCTACCCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAg                                                                                    <  2:7853200/93‑1 (MQ=255)

     gagaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAg                                                                                    <  2:7743752/93‑1 (MQ=255)

      agaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGa                                                                                   <  2:9204529/93‑1 (MQ=255)

       gaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaa                                                                                  >  1:7029356/1‑93 (MQ=255)

       gaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaa                                                                                  <  1:6913782/93‑1 (MQ=255)

       gaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaa                                                                                  <  1:4548984/93‑1 (MQ=255)

        aTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCCTGCTCACCACCCTGCTTGGCGAAGAAAGCTTCCAGaag                                                                                 >  1:3619905/1‑92 (MQ=255)

         tGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAg                                                                                <  2:7428264/93‑1 (MQ=255)

          gAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAgg                                                                               <  2:9324793/93‑1 (MQ=255)

             caacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGAt                                                                            <  2:5580032/93‑1 (MQ=255)

             caacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGAt                                                                            >  1:5827083/1‑93 (MQ=255)

                cTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCa                                                                         >  1:3290416/1‑93 (MQ=255)

                 ttCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAg                                                                        >  1:8312495/1‑93 (MQ=255)

                  tCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGc                                                                       <  1:4134356/93‑1 (MQ=255)

                      cacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTa                                                                   <  2:6957786/93‑1 (MQ=255)

                      cacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTa                                                                   <  1:6609506/93‑1 (MQ=255)

                      cacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTa                                                                   <  2:3809906/93‑1 (MQ=255)

                       acCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAt                                                                  <  1:9077984/93‑1 (MQ=255)

                        cccTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAtt                                                                 <  2:2835445/93‑1 (MQ=255)

                          cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               <  2:6215973/93‑1 (MQ=255)

                          cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               <  2:2537432/93‑1 (MQ=255)

                           tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCg                                                              >  2:9347649/1‑93 (MQ=255)

                            gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGa                                                             >  1:2167099/1‑93 (MQ=255)

                            gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGACGCAGCTTTATTTCGa                                                             >  1:7177896/1‑93 (MQ=255)

                            gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGAATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGa                                                             <  2:9524626/93‑1 (MQ=255)

                             aCCGTTTAGGAGAAGGGGGCGTGAGTGATTCGGATGAGCCACACTCTGCTTTGCGAGGAAAGCTGCCAGAAAGTGATGCAGCTTTATTTCGAg                                                            >  2:5776365/1‑93 (MQ=255)

                             aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAg                                                            <  2:8531699/93‑1 (MQ=255)

                               cGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCg                                                          >  2:5810193/1‑93 (MQ=255)

                                 tttACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTc                                                        <  2:6630540/93‑1 (MQ=255)

                                      gagaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgat                                                   <  2:8119766/93‑1 (MQ=255)

                                       agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  >  2:7548898/1‑93 (MQ=255)

                                       agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  <  1:1806657/93‑1 (MQ=255)

                                       agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  >  2:5026832/1‑93 (MQ=255)

                                        gaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATgg                                                 >  2:8353431/1‑93 (MQ=255)

                                         aaGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGt                                                <  1:7361288/93‑1 (MQ=255)

                                          aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               >  2:6515775/1‑93 (MQ=255)

                                          aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               <  2:2270451/93‑1 (MQ=255)

                                          aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               <  1:1322860/93‑1 (MQ=255)

                                           gggCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAg                                              >  2:5139300/1‑93 (MQ=255)

                                           gggCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAg                                              <  1:5662121/93‑1 (MQ=255)

                                           gggCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCGTTATGTTGAGCGTCATGATGGTAg                                              >  1:8980118/1‑93 (MQ=255)

                                             gcgcGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTg                                            <  1:4400121/93‑1 (MQ=255)

                                              cgcgGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGc                                           >  2:4386004/1‑93 (MQ=255)

                                                cgGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAg                                         <  1:7075715/93‑1 (MQ=255)

                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  2:4667086/1‑93 (MQ=255)

                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  2:2755735/1‑93 (MQ=255)

                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  2:9776788/1‑93 (MQ=255)

                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  1:1594620/1‑93 (MQ=255)

                                                   aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                      >  2:2992563/1‑93 (MQ=255)

                                                   aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                      <  2:767452/93‑1 (MQ=255)

                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    >  2:8832176/1‑93 (MQ=255)

                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  2:3544421/93‑1 (MQ=255)

                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  1:7675433/93‑1 (MQ=255)

                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  1:7948253/93‑1 (MQ=255)

                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  2:631339/93‑1 (MQ=255)

                                                       gATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtg                                  <  2:6580590/93‑1 (MQ=255)

                                                        aTTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtgt                                 <  1:8115499/93‑1 (MQ=255)

                                                        aTTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtgt                                 <  1:9640683/93‑1 (MQ=255)

                                                          tCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCCACCtgtgc                               >  2:5338084/1‑92 (MQ=255)

                                                             cATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacga                            >  1:4228384/1‑93 (MQ=255)

                                                              aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgac                           >  2:6455924/1‑93 (MQ=255)

                                                              aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgac                           >  1:1910322/1‑93 (MQ=255)

                                                               tGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACt                          >  2:3726183/1‑93 (MQ=255)

                                                               tGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACt                          >  2:6010215/1‑93 (MQ=255)

                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGTTGGTAGTGCAGCGACCTGTGACGACTTtg                       <  2:2008011/93‑1 (MQ=255)

                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       >  1:9924170/1‑93 (MQ=255)

                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       <  2:3201940/93‑1 (MQ=255)

                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       >  2:1556168/1‑93 (MQ=255)

                                                                    cacaCCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtgtg                     <  1:9318487/93‑1 (MQ=255)

                                                                      cacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                   >  1:971879/1‑93 (MQ=255)

                                                                      cacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                   >  2:3462913/1‑93 (MQ=255)

                                                                       acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  >  2:4548007/1‑93 (MQ=255)

                                                                       acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  >  2:4248290/1‑93 (MQ=255)

                                                                        cccTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAgg                 >  2:4522171/1‑93 (MQ=255)

                                                                        cccTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAgg                 >  1:4826714/1‑93 (MQ=255)

                                                                        cccTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAgg                 <  1:444484/93‑1 (MQ=255)

                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              >  2:1203225/1‑93 (MQ=255)

                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              <  1:9568798/93‑1 (MQ=255)

                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              <  1:4704301/93‑1 (MQ=255)

                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              >  2:2198631/1‑93 (MQ=255)

                                                                            gCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAt             >  1:5458809/1‑93 (MQ=255)

                                                                             cTTGGCGAAGAAAGCTTCCAGAAAGGGATGCATCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATg            <  1:636006/93‑1 (MQ=255)

                                                                             cTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATg            <  2:3429928/93‑1 (MQ=255)

                                                                               tGGCGAAGAAATCTTCCAGAAAGGCATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGa          <  1:4037507/93‑1 (MQ=255)

                                                                                 gCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAg        >  1:2881527/1‑93 (MQ=255)

                                                                                  cGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGa       <  1:8743703/93‑1 (MQ=255)

                                                                                   gaagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAt      >  1:5885835/1‑93 (MQ=255)

                                                                                   gaagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAt      <  1:2671850/93‑1 (MQ=255)

                                                                                    aagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATg     <  1:7138767/93‑1 (MQ=255)

                                                                                     agaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGc    <  1:6194991/93‑1 (MQ=255)

                                                                                     agaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGc    >  1:7113085/1‑93 (MQ=255)

                                                                                      gaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCg   <  2:8656285/93‑1 (MQ=255)

                                                                                      gaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCg   >  1:9256486/1‑93 (MQ=255)

TCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT  >  NC_000913/990950‑991129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`