BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,684	A→G	S18P (TCC→CCC)	ycbP ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,684	0	A	G	99.6%	740.1 / NA	230	S18P (TCC→CCC)	ycbP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (122/107); minor base T (0/1); total (122/108)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

GTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACA  >  NC_000913/996594‑996774

gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGc                                                                                          >  1:7937551/1‑93 (MQ=255)

gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGc                                                                                          <  1:4717399/93‑1 (MQ=255)

gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGc                                                                                          >  1:4654160/1‑93 (MQ=255)

gTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGc                                                                                          >  2:7513574/1‑93 (MQ=255)

 tAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCt                                                                                         >  2:8548727/1‑93 (MQ=255)

    aTCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTggg                                                                                      >  1:7319473/1‑92 (MQ=255)

     tCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAg                                                                                     >  2:6272049/1‑93 (MQ=255)

     tCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAg                                                                                     <  1:9306757/93‑1 (MQ=255)

     tCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAg                                                                                     <  1:2877819/93‑1 (MQ=255)

     tCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAg                                                                                     >  1:1678985/1‑93 (MQ=255)

      cTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGc                                                                                    <  2:2410711/93‑1 (MQ=255)

       ttCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCg                                                                                   >  2:9426312/1‑93 (MQ=255)

       ttCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCg                                                                                   >  2:1615825/1‑93 (MQ=255)

         ccGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCgag                                                                                 <  1:1752554/93‑1 (MQ=255)

           ggggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGaa                                                                               <  2:7427730/93‑1 (MQ=255)

           ggggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGaa                                                                               >  1:5438233/1‑93 (MQ=255)

            gggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAg                                                                              >  1:2635612/1‑93 (MQ=255)

            gggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAg                                                                              <  1:8638132/93‑1 (MQ=255)

            gggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAg                                                                              <  1:5181143/93‑1 (MQ=255)

            gggCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAg                                                                              >  2:60642/1‑93 (MQ=255)

             ggCTAAGTTGTGCAGATTGCAGTGATAAACCTCTACATCCAGGCCATGTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAgg                                                                             <  1:5253566/93‑1 (MQ=255)

              gCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGa                                                                            >  2:5879144/1‑93 (MQ=255)

                gAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGaaa                                                                          >  2:8595347/1‑93 (MQ=255)

                 aaGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAg                                                                         <  2:9642208/93‑1 (MQ=255)

                 aaGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAg                                                                         >  2:3614701/1‑93 (MQ=255)

                   gTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCg                                                                       <  2:2328717/93‑1 (MQ=255)

                    ttttGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGa                                                                      <  1:1025979/93‑1 (MQ=255)

                    ttttGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGa                                                                      >  1:8977322/1‑93 (MQ=255)

                      ttGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTGCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAgg                                                                    <  2:5136672/93‑1 (MQ=255)

                       tGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGa                                                                   >  1:3483919/1‑93 (MQ=255)

                             ttCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAccc                                                             <  1:9129034/93‑1 (MQ=255)

                             ttCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTAccc                                                             <  2:9921899/93‑1 (MQ=255)

                              tCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCg                                                            >  1:6594898/1‑93 (MQ=255)

                               ccAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGc                                                           >  2:7144798/1‑93 (MQ=255)

                                 aGTGATAACCCTCTACATCCAGGCAATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCa                                                         <  1:7793110/93‑1 (MQ=255)

                                 aGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCa                                                         >  2:9217865/1‑93 (MQ=255)

                                 aGTGAGAATCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCa                                                         <  2:9050218/93‑1 (MQ=255)

                                  gTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAg                                                        <  2:3928039/93‑1 (MQ=255)

                                  gTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAg                                                        <  2:8669832/93‑1 (MQ=255)

                                   tGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAgg                                                       <  1:7152319/93‑1 (MQ=255)

                                   tGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAgg                                                       <  1:5366228/93‑1 (MQ=255)

                                    gATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAggg                                                      >  2:2267007/1‑93 (MQ=255)

                                     aTAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGt                                                     <  1:2782311/93‑1 (MQ=255)

                                      tAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtg                                                    >  2:6553369/1‑93 (MQ=255)

                                      tAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtg                                                    >  2:7576169/1‑93 (MQ=255)

                                      tAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtg                                                    >  1:7519702/1‑93 (MQ=255)

                                       aaaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtga                                                   <  2:1863224/93‑1 (MQ=255)

                                       aaaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtga                                                   >  2:4158782/1‑93 (MQ=255)

                                       aaaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtga                                                   <  1:4087111/93‑1 (MQ=255)

                                        aaCCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgat                                                  <  2:7094370/93‑1 (MQ=255)

                                          ccTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgatga                                                <  1:2687048/93‑1 (MQ=255)

                                          ccTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgatga                                                >  1:2316489/1‑93 (MQ=255)

                                          ccTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGtgatga                                                >  2:4791809/1‑93 (MQ=255)

                                           ctctACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGac                                               <  2:7001718/93‑1 (MQ=255)

                                            tctACATCCAGGTCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGaca                                              <  1:6883454/93‑1 (MQ=255)

                                            tctACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGaca                                              >  2:3002525/1‑93 (MQ=255)

                                            tctACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGaca                                              >  2:4708266/1‑93 (MQ=255)

                                              tACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACg                                            >  1:1436380/1‑93 (MQ=255)

                                                cATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCa                                          <  1:4688422/93‑1 (MQ=255)

                                                cATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCa                                          <  1:3260514/93‑1 (MQ=255)

                                                cATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGGGATGACACGCa                                          >  2:2764886/1‑93 (MQ=255)

                                                 aTCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCAt                                         >  2:5541366/1‑93 (MQ=255)

                                                  tCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATa                                        >  2:6897502/1‑93 (MQ=255)

                                                    cAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATAct                                      >  1:8008380/1‑93 (MQ=255)

                                                    cAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATAct                                      <  1:8155775/93‑1 (MQ=255)

                                                     aGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATActc                                     >  2:6835799/1‑93 (MQ=255)

                                                      ggCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGTGTGATGACACGCATActct                                    <  1:6870569/93‑1 (MQ=255)

                                                         cATTTAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACtctctc                                 >  2:4301898/1‑93 (MQ=255)

                                                           tttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                               >  2:4747348/1‑93 (MQ=255)

                                                           tttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                               >  1:3086563/1‑93 (MQ=255)

                                                           tttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCt                               >  1:5587225/1‑93 (MQ=255)

                                                            ttAGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCtt                              >  2:4971743/1‑93 (MQ=255)

                                                            ttAGTTTTTCCCGCGAATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCtt                              >  1:7207186/1‑93 (MQ=255)

                                                              aGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTtat                            <  1:305199/93‑1 (MQ=255)

                                                              aGTTTTTCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTtat                            >  1:6089461/1‑93 (MQ=255)

                                                                  tttCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAAcc                        >  2:5089173/1‑93 (MQ=255)

                                                                   ttCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCa                       >  2:407611/1‑93 (MQ=255)

                                                                    tCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCaa                      <  2:8277060/93‑1 (MQ=255)

                                                                    tCCCGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCaa                      <  1:8597134/93‑1 (MQ=255)

                                                                     cccGCGCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAAt                     >  2:7899066/1‑93 (MQ=255)

                                                                      ccGCGCATATTCCAGCAAGGTGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATTACACGCATACTCTCTCCTTATAACCAAtt                    <  1:1761950/93‑1 (MQ=255)

                                                                       cgcgCATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTg                   >  2:10012311/1‑93 (MQ=255)

                                                                           cATATTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCt               <  2:1448286/93‑1 (MQ=255)

                                                                            atatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtt              >  1:9533192/1‑93 (MQ=255)

                                                                            atatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtt              >  2:2837032/1‑93 (MQ=255)

                                                                             tatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCttt             <  2:37926/93‑1 (MQ=255)

                                                                             tatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCttt             <  1:9904454/93‑1 (MQ=255)

                                                                             tatTCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCttt             >  2:7925789/1‑93 (MQ=255)

                                                                               ttCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCttttt           >  1:1145878/1‑93 (MQ=255)

                                                                               ttCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCttttt           >  2:67272/1‑93 (MQ=255)

                                                                                tCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtttttt          <  1:377659/93‑1 (MQ=255)

                                                                                tCCAGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCtttttt          <  1:3380864/93‑1 (MQ=255)

                                                                                   aGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGt       >  2:1085736/1‑93 (MQ=255)

                                                                                   aGCAAGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGt       <  2:8854927/93‑1 (MQ=255)

                                                                                      aaGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTaa    >  2:7619519/1‑93 (MQ=255)

                                                                                       aGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAc   <  1:9828681/93‑1 (MQ=255)

                                                                                       aGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAc   >  2:4222658/1‑93 (MQ=255)

                                                                                       aGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAc   <  1:6232937/93‑1 (MQ=255)

                                                                                       aGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAc   <  1:2228484/93‑1 (MQ=255)

                                                                                       aGGGGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAAc   <  2:6185180/93‑1 (MQ=255)

GTAGATCTTCCGGGGCGAAGTTTTGCAGATTCCAGTGATAAACCTCTACATCCAGGCCATTTAGTTTTTCCCGCGCATATTCCAGCAAGGAGCTGGAGCGAGAAGGAAAGCGAGGACTACCCGCCAGGGTGATGACACGCATACTCTCTCCTTATAACCAATTGTTCTTTTTTTGTTAACA  >  NC_000913/996594‑996774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`