BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,719	C→T	T48M (ACG→ATG)	hyaE →	HyaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,719	0	C	T	99.5%	734.2 / ‑5.7	202	T48M (ACG→ATG)	hyaE	HyaE
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (105/96); total (106/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 218 total aligned reads displayed.

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

gCTGGACGCCCGCCAGTGAATCCCGACTTGACGACTTGCTTACGCAAGCGTCAGACGGCGTGGTGATATTCAGGCGTGTCACGAAaagcatgc                                                                                         >  2:5006082/1‑93 (MQ=255)

gCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgcatgc                                                                                         >  1:9751530/1‑93 (MQ=255)

 cTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGcc                                                                                        <  1:7988225/93‑1 (MQ=255)

  tGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCa                                                                                       <  1:9411509/93‑1 (MQ=255)

   ggACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCag                                                                                      <  1:5454885/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  2:3235096/93‑1 (MQ=255)

     aCGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCagag                                                                                    >  1:7649018/1‑93 (MQ=255)

        ccAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTc                                                                                 <  1:8122185/93‑1 (MQ=255)

         cagtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCa                                                                                <  2:9664904/93‑1 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              >  2:7748187/1‑93 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              >  2:284938/1‑93 (MQ=255)

             cagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGa                                                                            >  2:5420963/1‑93 (MQ=255)

              agtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGAt                                                                           >  1:5577903/1‑93 (MQ=255)

               gtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATa                                                                          <  2:9779771/93‑1 (MQ=255)

                tGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         <  2:2355235/93‑1 (MQ=255)

                tGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         <  1:2393857/93‑1 (MQ=255)

                ccaaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCATTGACCCGAAACGCACGCCAGAGGTCAGCGATaa                                                                         >  2:5162426/3‑93 (MQ=255)

                ataaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         <  1:7026275/91‑1 (MQ=255)

                 gAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAAt                                                                        >  1:3736025/1‑93 (MQ=255)

                 gAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAAt                                                                        >  1:8440637/1‑93 (MQ=255)

                   aTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      >  2:5580739/1‑93 (MQ=255)

                    tCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     >  2:1588882/1‑93 (MQ=255)

                    tCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     <  1:1381485/93‑1 (MQ=255)

                    tCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     >  2:3323352/1‑93 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCgg                                                                    >  2:5927084/1‑93 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 <  1:8918532/93‑1 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 >  2:3705914/1‑93 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 <  1:2721502/93‑1 (MQ=255)

                           ttGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGa                                                              >  1:246863/1‑93 (MQ=255)

                           ttGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGa                                                              >  1:3975329/1‑93 (MQ=255)

                            tGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAt                                                             <  1:1351870/93‑1 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAtt                                                            <  1:7826933/93‑1 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAtt                                                            >  2:8333678/1‑93 (MQ=255)

                              acgacTGGCTTCCGCAAGCGCCAGAAGGCGCGGTGTTCTTAAGCAGTGACCCGAAACGCATGCCCGAGGTCAGCGATAATCCGGTAATGATTg                                                           >  1:395112/1‑93 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTAAGCGATAATCCGGTAATGATTGGc                                                         <  1:6105123/93‑1 (MQ=255)

                                   tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      <  1:2994545/93‑1 (MQ=255)

                                   tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      >  2:3974246/1‑93 (MQ=255)

                                    ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                                                     <  1:314384/93‑1 (MQ=255)

                                    ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                                                     <  2:3786230/93‑1 (MQ=255)

                                    ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                                                     <  1:5094691/93‑1 (MQ=255)

                                    ggCCTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                                                     <  1:9469821/93‑1 (MQ=255)

                                     gCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                                                    >  1:6406879/1‑93 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  2:424575/93‑1 (MQ=255)

                                       ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                                                  >  2:8556325/1‑93 (MQ=255)

                                        tACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACt                                                 >  1:2107275/1‑93 (MQ=255)

                                        tACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACt                                                 >  2:9246149/1‑93 (MQ=255)

                                          cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc                                               <  2:170615/93‑1 (MQ=255)

                                          cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc                                               >  1:8185225/1‑93 (MQ=255)

                                           gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg                                              >  1:4847312/1‑93 (MQ=255)

                                           gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg                                              <  2:3483611/93‑1 (MQ=255)

                                              aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGGTAATCCGGTAATGATTGGCGAATTACTGCGCGa                                           <  2:613227/93‑1 (MQ=255)

                                               gcgcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAg                                          >  1:3251807/1‑93 (MQ=255)

                                                 gcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGtt                                        >  1:2228727/1‑93 (MQ=255)

                                                  ccAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGttt                                       >  2:8877597/1‑93 (MQ=255)

                                                  ccAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGttt                                       <  2:8031417/93‑1 (MQ=255)

                                                    aGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc                                     >  1:4086365/1‑93 (MQ=255)

                                                    aGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc                                     >  1:5953839/1‑93 (MQ=255)

                                                        ggCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc                                 <  1:308978/93‑1 (MQ=255)

                                                         gCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACt                                >  1:1199583/1‑93 (MQ=255)

                                                         gCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACt                                >  2:1024567/1‑93 (MQ=255)

                                                            tggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtata                             >  1:9923632/1‑93 (MQ=255)

                                                            tggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtata                             <  1:1670542/93‑1 (MQ=255)

                                                              gtgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACa                           >  2:5918028/1‑93 (MQ=255)

                                                              gtgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACa                           <  2:2089900/93‑1 (MQ=255)

                                                              gtgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACa                           <  1:9246124/93‑1 (MQ=255)

                                                              gtgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACa                           <  1:130199/93‑1 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          <  2:6336728/93‑1 (MQ=255)

                                                                 ttattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATgg                        <  2:2963977/93‑1 (MQ=255)

                                                                  tattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGc                       >  2:794380/1‑93 (MQ=255)

                                                                   attaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCa                      >  2:8374585/1‑93 (MQ=255)

                                                                    ttaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAg                     <  2:7150485/93‑1 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCGAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGt                   <  1:9308050/93‑1 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGt                   <  1:8235230/93‑1 (MQ=255)

                                                                       aGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTg                  <  2:9979559/93‑1 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 <  1:9001411/93‑1 (MQ=255)

                                                                         cAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTggg                >  2:3661975/1‑92 (MQ=255)

                                                                         cAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGc                <  1:677794/93‑1 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCg               >  1:2904925/1‑93 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCg               >  2:7047872/1‑93 (MQ=255)

                                                                           gTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAAATGGCAGGTGGCGa              <  1:5663428/93‑1 (MQ=255)

                                                                            tGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAt             >  2:1762003/1‑93 (MQ=255)

                                                                            tGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAt             <  1:8821029/93‑1 (MQ=255)

                                                                             gACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAtt            <  1:3189776/93‑1 (MQ=255)

                                                                             gACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAtt            <  2:1317147/93‑1 (MQ=255)

                                                                             gACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAtt            <  2:7295819/93‑1 (MQ=255)

                                                                              aCCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTg           <  2:4861349/93‑1 (MQ=255)

                                                                               cccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGc          >  2:7635705/1‑93 (MQ=255)

                                                                                 cGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAAATGGCAGGTGGCGAttctta        >  1:6492706/1‑89 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       <  1:1549033/93‑1 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       <  1:5613795/93‑1 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      >  1:5218832/1‑93 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      <  2:2918424/93‑1 (MQ=255)

                                                                                    aaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc     >  2:2574548/1‑93 (MQ=255)

                                                                                    aaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc     >  2:5042306/1‑93 (MQ=255)

                                                                                     acgcatgcCAGAGTGCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    <  2:2190040/93‑1 (MQ=255)

                                                                                     acgcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    >  1:9648527/1‑93 (MQ=255)

                                                                                     acgcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    <  1:4343180/93‑1 (MQ=255)

                                                                                     acgcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCt    <  2:9410369/93‑1 (MQ=255)

                                                                                       gcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg  <  2:7390665/93‑1 (MQ=255)

                                                                                       gcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg  >  1:3464694/1‑93 (MQ=255)

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`