BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,815	C→T	S61L (TCG→TTG)	yabB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,815	0	C	T	99.5%	1501.0 / ‑5.1	405	S61L (TCG→TTG)	yabB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (214/189); total (214/191)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 422 total aligned reads displayed.

CCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTC  >  NC_000913/89725‑89904

ccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgc                                                                                         <  1:6160721/93‑1 (MQ=255)

 cGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgcg                                                                                        <  1:540344/93‑1 (MQ=255)

  ggTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGt                                                                                       >  2:2889513/1‑93 (MQ=255)

  ggTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGt                                                                                       >  1:4564390/1‑93 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTCCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  2:4220562/93‑1 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      >  1:7104034/1‑93 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      >  1:6235410/1‑93 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  2:1696933/93‑1 (MQ=255)

    tCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCt                                                                                     >  2:6535922/1‑93 (MQ=255)

     cAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTg                                                                                    >  1:9174213/1‑93 (MQ=255)

     cAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTg                                                                                    <  2:7651186/93‑1 (MQ=255)

      aaaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGt                                                                                   >  2:3438412/1‑93 (MQ=255)

       aaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTc                                                                                  <  1:8099737/93‑1 (MQ=255)

       aaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTc                                                                                  >  1:65223/1‑93 (MQ=255)

        aTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTct                                                                                 >  1:4228514/1‑92 (MQ=255)

        aTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCg                                                                                 <  1:5923466/93‑1 (MQ=255)

         tGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGa                                                                                <  2:5743909/93‑1 (MQ=255)

         tGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGa                                                                                >  1:4769093/1‑93 (MQ=255)

         tGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGa                                                                                <  2:7834197/93‑1 (MQ=255)

           gTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGc                                                                              >  2:7289097/1‑93 (MQ=255)

            tttGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCa                                                                             <  1:6990918/93‑1 (MQ=255)

             ttGCTCCAGTGACATTTATCGACCGTGCCGGCTGCGGTACCCCCTTCCTGAATGGGAAATTATCAAGCAAAAATTATGGCGTCTGTGGAGccg                                                                            <  2:6805057/93‑3 (MQ=255)

               gCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGa                                                                          <  1:4827796/93‑1 (MQ=255)

                cACCATTGACATTTATCACCCGTACCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGaa                                                                         <  2:5494407/93‑1 (MQ=255)

                 aCCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAc                                                                        <  1:912776/93‑1 (MQ=255)

                  ccATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAcc                                                                       <  2:8819235/93‑1 (MQ=255)

                  ccATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAcc                                                                       <  2:4039788/93‑1 (MQ=255)

                   cATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAACATTATTGCGTCTGTCGAGCATGAAccc                                                                      >  1:7504474/1‑93 (MQ=255)

                   cATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAccc                                                                      >  1:9967602/1‑93 (MQ=255)

                    aTTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCg                                                                     <  1:3947416/93‑1 (MQ=255)

                     ttGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCgg                                                                    >  1:5543083/1‑93 (MQ=255)

                      tGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGt                                                                   >  1:9396118/1‑93 (MQ=255)

                          aTTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAg                                                               <  2:3773099/93‑1 (MQ=255)

                           tttATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTAGAGCATGAACCCGGTTGAgc                                                              <  1:3108046/93‑1 (MQ=255)

                              aTCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGcc                                                           >  2:8388537/1‑93 (MQ=255)

                                ccgccGTGCCTGCTGCTTTAACCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgt                                                         <  1:3464435/90‑1 (MQ=255)

                                cACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgt                                                         <  1:3603424/93‑1 (MQ=255)

                                  cccGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgtgt                                                       >  2:3279918/1‑93 (MQ=255)

                                  cccGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgtgt                                                       <  2:1082171/93‑1 (MQ=255)

                                   ccGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGtgtg                                                      >  1:2863038/1‑93 (MQ=255)

                                    cGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGc                                                     <  1:6859715/93‑1 (MQ=255)

                                    cGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGc                                                     >  2:7528037/1‑93 (MQ=255)

                                    cGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGc                                                     <  1:1234517/93‑1 (MQ=255)

                                     gTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCa                                                    >  2:481901/1‑93 (MQ=255)

                                       gCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgc                                                  <  2:6244371/93‑1 (MQ=255)

                                         ctgctgCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgcgc                                                >  1:5719853/1‑93 (MQ=255)

                                         ctgctgCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgcgc                                                >  2:1945007/1‑93 (MQ=255)

                                          tgctgcTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGcc                                               >  1:857295/1‑93 (MQ=255)

                                           gctgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCt                                              >  2:4930004/1‑93 (MQ=255)

                                           gctgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCt                                              <  2:6675228/93‑1 (MQ=255)

                                            ctgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTa                                             <  1:725445/93‑1 (MQ=255)

                                             tgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTAc                                            <  2:945260/93‑1 (MQ=255)

                                              gctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACt                                           >  1:8216225/1‑93 (MQ=255)

                                              gctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACt                                           <  2:1592830/93‑1 (MQ=255)

                                              gctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACt                                           <  1:671031/93‑1 (MQ=255)

                                                tttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                                         >  2:4573061/1‑93 (MQ=255)

                                                tttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                                         >  2:6948001/1‑93 (MQ=255)

                                                tttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                                         >  2:3986472/1‑93 (MQ=255)

                                                  tACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTa                                       <  1:2201578/93‑1 (MQ=255)

                                                   aCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAg                                      <  2:5290850/93‑1 (MQ=255)

                                                   aCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAg                                      <  1:1023017/93‑1 (MQ=255)

                                                    cccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAgg                                     <  1:7733472/93‑1 (MQ=255)

                                                      cccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTc                                   >  1:1799916/1‑93 (MQ=255)

                                                       cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                                  >  1:1433074/1‑93 (MQ=255)

                                                       cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                                  <  2:1021968/93‑1 (MQ=255)

                                                       cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                                  <  1:8900243/93‑1 (MQ=255)

                                                         tgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATg                                >  2:8234125/1‑93 (MQ=255)

                                                          gcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGc                               >  2:2405694/1‑93 (MQ=255)

                                                          gcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGc                               >  1:7420140/1‑93 (MQ=255)

                                                            ctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCa                             <  2:9026500/93‑1 (MQ=255)

                                                             tgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg                            >  1:4451572/1‑93 (MQ=255)

                                                               aaTGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCg                          >  2:8088352/1‑93 (MQ=255)

                                                               aaTGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCg                          >  1:9525990/1‑93 (MQ=255)

                                                                aTGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGa                         >  1:8416794/1‑93 (MQ=255)

                                                                 tGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGaa                        <  1:6363526/93‑1 (MQ=255)

                                                                 tGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGaa                        <  2:3917575/93‑1 (MQ=255)

                                                                 tGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGaa                        <  2:3121645/93‑1 (MQ=255)

                                                                  gggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAAt                       <  2:1154686/93‑1 (MQ=255)

                                                                   ggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATg                      <  1:9904425/93‑1 (MQ=255)

                                                                       aTTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAg                  >  1:2022733/1‑93 (MQ=255)

                                                                        ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga                 >  2:9832879/1‑93 (MQ=255)

                                                                        ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga                 >  2:1452022/1‑93 (MQ=255)

                                                                        ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga                 >  2:3618547/1‑93 (MQ=255)

                                                                         tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTCCTGTTAGGTCATGCCAGCGAATGTAAgag                >  1:2512674/1‑92 (MQ=255)

                                                                         tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgat                >  2:5443801/1‑93 (MQ=255)

                                                                         tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgat                >  2:7158730/1‑93 (MQ=255)

                                                                         tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgat                <  2:5694143/93‑1 (MQ=255)

                                                                         tATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgat                <  2:5994761/93‑1 (MQ=255)

                                                                          aTCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTCGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatg               <  1:6022955/93‑1 (MQ=255)

                                                                          aTCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatg               <  1:1637849/93‑1 (MQ=255)

                                                                          aTCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatg               <  1:511535/93‑1 (MQ=255)

                                                                            caaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgga             <  2:2618538/91‑1 (MQ=255)

                                                                            caaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgga             <  2:8608036/91‑1 (MQ=255)

                                                                              aGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatggatg           >  2:9276338/1‑93 (MQ=255)

                                                                               gCAAAAATTATTGCGTCTGTCGAGCATGAGCCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGatggatgg          >  2:6222100/1‑93 (MQ=255)

                                                                                  aaaaTTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGgcgc       >  1:3801720/1‑93 (MQ=255)

                                                                                   aaaTTATTGCGTCTGTCGAGCATGAGCCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCa      <  2:122315/93‑1 (MQ=255)

                                                                                   aaaTTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCa      <  2:9712045/93‑1 (MQ=255)

                                                                                      ttATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGt   <  2:6361996/93‑1 (MQ=255)

                                                                                      ttATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGt   >  2:212469/1‑93 (MQ=255)

CCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTC  >  NC_000913/89725‑89904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`