Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 102,844 A→G Q204Q (CAA→CAG)  ddlB → D‑alanine‑D‑alanine ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913102,8440AG99.8% 1330.2 / ‑5.5 407Q204Q (CAA→CAG) ddlBD‑alanine‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (193/212);  total (194/213)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

Only 100 of 417 total aligned reads displayed.

TGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGA  >  NC_000913/102754‑102933
                                                                                          |                                                                                         
 gaagaaGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCg                                                                                        >  2:4604408/1‑93 (MQ=255)
 gaagaaGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCg                                                                                        >  1:8031415/1‑93 (MQ=255)
   agaagTATTTATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTc                                                                                      <  1:7830935/93‑1 (MQ=255)
   agaagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTc                                                                                      <  1:1917783/93‑1 (MQ=255)
     aagTATTGATTGAAAGATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    <  2:5128163/93‑1 (MQ=255)
     aagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    <  1:213665/93‑1 (MQ=255)
     aagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    >  1:7494459/1‑93 (MQ=255)
     aagTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    >  1:5288960/1‑93 (MQ=255)
     aagTATTGATTGAAAAATGGCNAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCg                                                                                    <  1:1794150/93‑1 (MQ=255)
       gTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGa                                                                                  <  1:8901225/93‑1 (MQ=255)
       gTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGa                                                                                  >  1:6097597/1‑93 (MQ=255)
         attgattgAAAAATGGCTAAGAGGGCCGGAGTTCACGGCTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCAGTCCGGCAc                                                                                >  1:1277490/1‑93 (MQ=255)
          ttgattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAAcc                                                                               >  2:6265102/1‑93 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             >  2:7930369/1‑93 (MQ=255)
            gattgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCtt                                                                             <  2:6145233/93‑1 (MQ=255)
               tgaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTa                                                                          >  1:8201269/1‑93 (MQ=255)
                gaAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTAt                                                                         <  1:5434070/93‑1 (MQ=255)
                  aaaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTG‑AGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      >  1:967153/1‑93 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      >  1:2349899/1‑93 (MQ=255)
                   aaaTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGAt                                                                      >  2:4639288/1‑93 (MQ=255)
                     aTGGCTAAGTGGGGCTGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  2:143543/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATCCGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  1:9052750/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  1:8256577/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  2:7752733/93‑1 (MQ=255)
                     aTGGCTAAGTGGGCCGGAGTTCACGCTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTa                                                                    <  1:139537/93‑1 (MQ=255)
                       ggCTAAGTGGGCCGGTGTTCACGGTTGCGATGCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATg                                                                  <  2:2640013/93‑1 (MQ=255)
                       ggCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATg                                                                  <  1:5210065/93‑1 (MQ=255)
                        gCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGa                                                                 <  1:4871572/93‑1 (MQ=255)
                        gCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGa                                                                 >  2:7272000/1‑93 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATGCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgg                                                               <  2:2853407/93‑1 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgg                                                               <  2:2062695/93‑1 (MQ=255)
                          tAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAgg                                                               >  1:7275071/1‑93 (MQ=255)
                           aaGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGc                                                              >  1:1693008/1‑93 (MQ=255)
                            aGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCg                                                             >  1:5855065/1‑93 (MQ=255)
                            aGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCg                                                             >  2:277183/1‑93 (MQ=255)
                              tGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGaa                                                           >  2:1527035/1‑93 (MQ=255)
                                ggCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGt                                                         >  2:2469186/1‑93 (MQ=255)
                                 gCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTa                                                        <  1:3444756/93‑1 (MQ=255)
                                  cccgAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAt                                                       <  1:2642182/90‑1 (MQ=255)
                                  ccGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAt                                                       <  2:4742720/93‑1 (MQ=255)
                                   cGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtc                                                      <  1:6597419/93‑1 (MQ=255)
                                    ggAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtct                                                     >  2:5671366/1‑93 (MQ=255)
                                     gAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctc                                                    <  2:7083433/93‑1 (MQ=255)
                                     gAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctc                                                    <  2:1076692/93‑1 (MQ=255)
                                      gggtCACGGTTGCGATACTCGGTGAAGAAATTTTCCCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctct                                                   <  1:2251010/90‑1 (MQ=255)
                                      aGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctct                                                   <  2:297976/93‑1 (MQ=255)
                                       gTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTAtctctc                                                  <  2:235094/93‑1 (MQ=255)
                                        ttCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATctctct                                                 >  2:8851322/1‑93 (MQ=255)
                                        ttCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATctctct                                                 >  1:4902773/1‑93 (MQ=255)
                                         tCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtg                                                <  2:5987313/93‑1 (MQ=255)
                                           aCGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgat                                              <  1:10002131/93‑1 (MQ=255)
                                             ggTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCtgatga                                            >  2:4940424/1‑93 (MQ=255)
                                              gTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATgag                                           >  2:6727483/1‑93 (MQ=255)
                                               ttGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATgaga                                          <  1:7163668/93‑1 (MQ=255)
                                                tGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGac                                         <  2:6973956/93‑1 (MQ=255)
                                                tGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGac                                         <  1:984281/93‑1 (MQ=255)
                                                 gCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGaca                                        <  2:8446728/93‑1 (MQ=255)
                                                    aTACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAg                                     <  2:7428489/93‑1 (MQ=255)
                                                      aCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAgat                                   >  1:8751248/1‑91 (MQ=255)
                                                      aCTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTa                                   <  1:6956521/93‑1 (MQ=255)
                                                       cTCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAt                                  >  2:9911310/1‑93 (MQ=255)
                                                        tCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAtt                                 >  1:8992873/1‑93 (MQ=255)
                                                        tCGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAtt                                 >  1:4286516/1‑93 (MQ=255)
                                                         cGGTGAAGAAATTTTACCGTCAATGCGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAttt                                <  2:1742300/93‑1 (MQ=255)
                                                         cGGTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTAttt                                <  1:3061205/93‑1 (MQ=255)
                                                          ggTGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTc                               >  1:3691107/1‑93 (MQ=255)
                                                            tGAAGAAATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTg                             >  1:94658/1‑93 (MQ=255)
                                                             gaagaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGc                            >  2:5559759/1‑93 (MQ=255)
                                                                gaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcccc                         >  1:4197454/1‑93 (MQ=255)
                                                                gaaATTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGcccc                         <  2:4464013/93‑1 (MQ=255)
                                                                 aaaTTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCg                        >  1:4088162/1‑93 (MQ=255)
                                                                 aaaTTTAACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCg                        >  2:6800137/1‑93 (MQ=255)
                                                                  aaTTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGc                       <  1:6766711/93‑1 (MQ=255)
                                                                   aTTTTACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCa                      >  1:4536148/1‑93 (MQ=255)
                                                                     tttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTTTGTTGTGACACAGTATTTTTGGTCCGCAgg                    >  2:8289845/1‑93 (MQ=255)
                                                                     tttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAgg                    <  1:5758114/93‑1 (MQ=255)
                                                                      ttACCGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGt                   <  2:4053776/93‑1 (MQ=255)
                                                                          cGTCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTgg               >  2:6584184/1‑93 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGaa             <  2:5855064/93‑1 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGaa             >  1:3270603/1‑93 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGaa             <  2:3533833/93‑1 (MQ=255)
                                                                            tCAATACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGaa             >  2:3896611/1‑93 (MQ=255)
                                                                              aaTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGc           >  1:645305/1‑93 (MQ=255)
                                                                              aaTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGc           >  2:3305977/1‑93 (MQ=255)
                                                                               aTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCg          >  2:1985803/1‑93 (MQ=255)
                                                                               aTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCg          >  2:9171802/1‑93 (MQ=255)
                                                                               aTACGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCg          <  1:2518121/93‑1 (MQ=255)
                                                                                 aCGTATTCAGCCGTCCGGATCCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTc        <  2:8956464/93‑1 (MQ=255)
                                                                                 aCGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTc        <  1:4762253/93‑1 (MQ=255)
                                                                                 aCGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTc        <  1:5455371/93‑1 (MQ=255)
                                                                                  cGTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTca       >  1:7914173/1‑93 (MQ=255)
                                                                                   gTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTcac      >  1:7526335/1‑93 (MQ=255)
                                                                                   gTATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTcac      >  1:8533362/1‑93 (MQ=255)
                                                                                    tATTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTcaca     >  2:4125876/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    >  1:8613611/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    >  1:2980105/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    >  2:9346320/1‑93 (MQ=255)
                                                                                     aTTCAGCCGTCCGGAACCTTCTACGATTATGAGACGAAGTTTCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACaa    <  2:3698181/93‑1 (MQ=255)
                                                                                       tCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAaga  >  2:5331505/1‑93 (MQ=255)
                                                                                       tCAGCCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAaga  <  1:2796327/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGAAGTATTGATTGAAAAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCAATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAGACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGA  >  NC_000913/102754‑102933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑