BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,527	A→G	K293R (AAG→AGG)	ycfU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,527	0	A	G	99.6%	764.2 / ‑4.7	240	K293R (AAG→AGG)	ycfU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (115/124); total (115/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

Only 100 of 250 total aligned reads displayed.

AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA  >  NC_000913/1175437‑1175616

aaaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGc                                                                                         <  2:5857373/93‑1 (MQ=255)

 aaaaaaTATCATGGGTTTACTTCTGAGCCTGATTGTCGCCGTGGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCa                                                                                        <  1:2758881/93‑1 (MQ=255)

     aaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGc                                                                                    >  1:10022929/1‑93 (MQ=255)

     aaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGc                                                                                    >  1:6354706/1‑93 (MQ=255)

        atgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGaa                                                                                 <  1:7298312/93‑1 (MQ=255)

         tgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAg                                                                                <  2:7032867/93‑1 (MQ=255)

         tgatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAg                                                                                >  1:9343776/1‑93 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               <  1:1054049/93‑1 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               >  1:9786229/1‑93 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               >  1:2807290/1‑93 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               <  1:3153620/93‑1 (MQ=255)

          gatgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGt                                                                               <  1:6051850/93‑1 (MQ=255)

            tgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTTATGGTAATGGAGAGGCAGGGCGAAGTAg                                                                             >  1:8943075/1‑93 (MQ=255)

            tgGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAg                                                                             <  1:2282092/93‑1 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  1:9912254/1‑93 (MQ=255)

                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGAt                                                                         >  1:4790943/1‑93 (MQ=255)

                 ttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCGCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATc                                                                        >  1:8462226/1‑93 (MQ=255)

                 ttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATc                                                                        <  1:5242872/93‑1 (MQ=255)

                  tACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATcc                                                                       >  2:8887529/1‑93 (MQ=255)

                   aCTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCt                                                                      >  2:3937465/1‑93 (MQ=255)

                     cgctgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGc                                                                    <  2:7309181/92‑1 (MQ=255)

                      tcggAGCCGGATTGTCGGCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCa                                                                   <  1:7825263/90‑1 (MQ=255)

                      gctgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCa                                                                   >  2:5246362/1‑93 (MQ=255)

                        tgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCaaa                                                                 >  1:4683322/1‑93 (MQ=255)

                        tgAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCaaa                                                                 >  2:9787905/1‑93 (MQ=255)

                         gAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTCCAAAc                                                                >  2:6071670/1‑93 (MQ=255)

                           gCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGc                                                              <  2:2454108/93‑1 (MQ=255)

                             cTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGACGTAGCGATCCTGCAAACGCaa                                                            <  1:1788199/93‑1 (MQ=255)

                             cTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCaa                                                            <  1:2866243/93‑1 (MQ=255)

                              tGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAg                                                           >  2:7561495/1‑93 (MQ=255)

                               gATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAgg                                                          >  2:3178373/1‑93 (MQ=255)

                               gATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAgg                                                          <  1:4853296/93‑1 (MQ=255)

                                aTTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGc                                                         <  2:3019857/93‑1 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        >  2:7966436/1‑93 (MQ=255)

                                 ttGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                        >  1:9013180/1‑93 (MQ=255)

                                   gTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTa                                                      >  2:1537021/1‑93 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     <  1:8328652/93‑1 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     <  1:1975076/93‑1 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     >  2:4187268/1‑93 (MQ=255)

                                    tCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                     <  1:5537343/93‑1 (MQ=255)

                                     cGCCGTTGCGGCGTTTAACATTATTACCTCCCTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                                    <  2:5344186/93‑1 (MQ=255)

                                     cGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                                    <  1:1332203/93‑1 (MQ=255)

                                     cGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc                                                    <  2:1916322/93‑1 (MQ=255)

                                        cGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTcc                                                 >  2:3117615/1‑93 (MQ=255)

                                         gTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCcg                                                <  2:8189382/93‑1 (MQ=255)

                                            gcggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGa                                             <  2:3540422/93‑1 (MQ=255)

                                             cggcgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGAc                                            <  2:5619708/93‑1 (MQ=255)

                                                cgTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACaaa                                         <  2:8945589/93‑1 (MQ=255)

                                                  tttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAAta                                       >  1:493713/1‑92 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCGAGGCTTAACTCCGCGACAAATCa                                      >  2:5521320/1‑93 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      <  1:4081955/93‑1 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      >  2:6279885/1‑93 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      <  2:8322731/93‑1 (MQ=255)

                                                   ttAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGGAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCa                                      >  1:3221970/1‑93 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     >  1:698429/1‑93 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     >  1:2466564/1‑93 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     <  1:5113429/93‑1 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCat                                     <  1:8802379/93‑1 (MQ=255)

                                                    tAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTGAACCCCGCGACAAATCaa                                     >  2:7717521/1‑92 (MQ=255)

                                                     aaCAATAATACCTCACTAGAACCGATGGTAATGGAGAGGCAAGGCGAAGTAGCGATCCTGCAAACGCAAGAATTACCTCCGCGACAAATCaag                                    <  2:9232994/93‑3 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   >  2:6181668/1‑93 (MQ=255)

                                                      aCATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatga                                   <  1:8688688/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCattat                                  <  2:5159084/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                                  <  2:4710609/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                                  <  1:4342895/93‑1 (MQ=255)

                                                       cATTATTACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgat                                  <  1:3599993/93‑1 (MQ=255)

                                                        attattACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgatg                                 >  1:7450033/1‑93 (MQ=255)

                                                        attattACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgatg                                 >  1:2267652/1‑93 (MQ=255)

                                                        attattACCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatgatg                                 >  2:5691135/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  1:797020/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  2:9090801/1‑93 (MQ=255)

                                                         ttattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATgg                                >  1:7538049/1‑93 (MQ=255)

                                                          tattaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGt                               >  2:559076/1‑93 (MQ=255)

                                                           attaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTc                              <  2:2764383/93‑1 (MQ=255)

                                                            ttaCCTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCt                             <  1:7950666/93‑1 (MQ=255)

                                                               ccTCACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                          <  2:5942712/93‑1 (MQ=255)

                                                                 tCACTAGGGCTGATGGTAATGGAGAGGCATGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATg                        <  2:7425870/93‑1 (MQ=255)

                                                                  cACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATgg                       <  1:5646321/93‑1 (MQ=255)

                                                                  cACTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATgg                       <  2:6892134/93‑1 (MQ=255)

                                                                    cTAGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTg                     >  1:5127409/1‑93 (MQ=255)

                                                                      aGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTACCTCCGCGACAAATCATGATGGTCTTTATGGTGCa                   <  1:7833157/93‑1 (MQ=255)

                                                                      aGGGCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTCCa                   >  2:8503544/1‑93 (MQ=255)

                                                                         gCTGATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgg                <  2:5938606/93‑1 (MQ=255)

                                                                            gATGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGc             >  1:6158331/1‑93 (MQ=255)

                                                                             aTGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGcc            >  2:2317830/1‑93 (MQ=255)

                                                                             aTGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGcc            >  1:9762348/1‑93 (MQ=255)

                                                                              tGGTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCa           <  1:9415622/93‑1 (MQ=255)

                                                                               ggTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAg          <  2:3563567/93‑1 (MQ=255)

                                                                                gTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc         <  2:4364509/93‑1 (MQ=255)

                                                                                gTAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc         <  1:1346959/93‑1 (MQ=255)

                                                                                 tAATGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcg        >  2:4573455/1‑93 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       <  2:4645696/93‑1 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       >  2:6619547/1‑93 (MQ=255)

                                                                                  aaTGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcgc       >  2:9387672/1‑93 (MQ=255)

                                                                                    tGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCg     >  2:199271/1‑93 (MQ=255)

                                                                                    tGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCg     >  1:5337460/1‑93 (MQ=255)

                                                                                    tGGAGAGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCg     >  2:1027546/1‑93 (MQ=255)

                                                                                      gagaGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCggg   >  1:6292761/1‑93 (MQ=255)

                                                                                      gagaGGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCggg   >  2:1221243/1‑93 (MQ=255)

                                                                                       agagGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGa  <  1:3193158/93‑1 (MQ=255)

AAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGA  >  NC_000913/1175437‑1175616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`