BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,238	A→G	V121A (GTG→GCG)	yacF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,238	0	A	G	99.4%	1107.3 / ‑5.1	343	V121A (GTG→GCG)	yacF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (194/146); total (194/149)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

TCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATT  >  NC_000913/112148‑112327

tCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGGGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGcc                                                                                         >  1:419092/1‑93 (MQ=255)

      tGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCa                                                                                   >  1:1982709/1‑93 (MQ=255)

      tGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCa                                                                                   <  2:1892678/93‑1 (MQ=255)

        cgcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAAt                                                                                 <  1:6528631/93‑1 (MQ=255)

        cgcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAAt                                                                                 <  2:8515058/93‑1 (MQ=255)

         gcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATc                                                                                <  1:49240/93‑1 (MQ=255)

           cTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaa                                                                              <  1:3964246/93‑1 (MQ=255)

            tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                             <  1:7346806/93‑1 (MQ=255)

            tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                             >  2:7212021/1‑93 (MQ=255)

             ggggTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAAc                                                                            >  2:460166/1‑93 (MQ=255)

             ggggTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAAc                                                                            >  2:7328863/1‑93 (MQ=255)

               ggTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGa                                                                          >  1:530728/1‑93 (MQ=255)

                gTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGAt                                                                         >  2:5310555/1‑93 (MQ=255)

                gTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGAt                                                                         >  2:7444396/1‑93 (MQ=255)

                  aGATTCGGCCAAATATGCAATGTACGTAAATCAAAGCTGTAAAAGCAGCCTCGGATGCTCAGTCGCTGTCGTTCCAGAGCAATCAAACGACCt                                                                       <  2:8681427/93‑1 (MQ=255)

                  aGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCt                                                                       >  2:6872720/1‑93 (MQ=255)

                  aGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCt                                                                       >  2:7575990/1‑93 (MQ=255)

                   gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                      >  2:1296184/1‑93 (MQ=255)

                    aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTc                                                                     >  2:2471510/1‑93 (MQ=255)

                    aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTc                                                                     <  2:2167668/93‑1 (MQ=255)

                    aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTc                                                                     >  1:8363266/1‑93 (MQ=255)

                     tGCAGCCAAATGTGCAATGTAGGCAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCa                                                                    >  1:6880118/1‑93 (MQ=255)

                      gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   <  1:6023054/93‑1 (MQ=255)

                      gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   >  2:76140/1‑93 (MQ=255)

                         gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCa                                                                <  1:4722947/93‑1 (MQ=255)

                           cAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGCCGCGCCAGCGCAATCAAACGATCTTCACGCAGa                                                              >  1:7772499/1‑93 (MQ=255)

                            aaaTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaa                                                             >  1:1194084/1‑93 (MQ=255)

                             aaTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaaa                                                            >  1:4057504/1‑93 (MQ=255)

                             aaTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaaa                                                            >  1:7519956/1‑93 (MQ=255)

                              aTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAt                                                           >  1:3205414/1‑93 (MQ=255)

                              aTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAt                                                           <  1:3142570/93‑1 (MQ=255)

                              aTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAt                                                           <  2:4934090/93‑1 (MQ=255)

                              aTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAt                                                           <  1:8694465/93‑1 (MQ=255)

                              aTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAt                                                           <  1:10014057/93‑1 (MQ=255)

                               tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                          <  1:1637850/93‑1 (MQ=255)

                               tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                          >  2:9048635/1‑93 (MQ=255)

                               tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                          <  1:5706804/93‑1 (MQ=255)

                               tgtgCAATGAAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaaaat                                                          >  2:6373681/1‑91 (MQ=255)

                                  gCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGcc                                                       >  1:9505729/1‑93 (MQ=255)

                                    aaTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCg                                                     <  2:5547158/93‑1 (MQ=255)

                                     aTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGa                                                    >  2:8324340/1‑93 (MQ=255)

                                      tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   <  1:760583/93‑1 (MQ=255)

                                      tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   >  2:2770132/1‑93 (MQ=255)

                                      tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   >  1:2252859/1‑93 (MQ=255)

                                      tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   <  2:4346090/93‑1 (MQ=255)

                                      tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   >  1:2762407/1‑93 (MQ=255)

                                      tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   >  2:6084580/1‑93 (MQ=255)

                                      agTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   <  1:2978687/92‑1 (MQ=255)

                                       gTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATa                                                  <  1:6790671/93‑1 (MQ=255)

                                       gTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATa                                                  <  1:3349915/93‑1 (MQ=255)

                                       gTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATa                                                  <  2:5415605/93‑1 (MQ=255)

                                        tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAc                                                 >  2:2567029/1‑93 (MQ=255)

                                         aGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                >  1:9000421/1‑93 (MQ=255)

                                          ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgg                                               >  2:4677738/1‑92 (MQ=255)

                                            tAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcgg                                             <  1:5078297/93‑1 (MQ=255)

                                             aaaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggc                                            <  2:5739844/93‑1 (MQ=255)

                                              aaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcg                                           <  2:10008436/93‑1 (MQ=255)

                                               aTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgc                                          <  1:4453422/93‑1 (MQ=255)

                                                 cAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcgg                                        <  1:6108522/93‑1 (MQ=255)

                                                   aaGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaa                                      >  2:7060192/1‑93 (MQ=255)

                                                    aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                     <  2:2405259/93‑1 (MQ=255)

                                                    aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                     <  1:9641069/93‑1 (MQ=255)

                                                     gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAAt                                    >  2:3696962/1‑93 (MQ=255)

                                                      cTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAAtt                                   >  2:4502151/1‑93 (MQ=255)

                                                       tGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTa                                  <  2:2102215/93‑1 (MQ=255)

                                                        gCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGCAATTaa                                 >  2:79344/1‑93 (MQ=255)

                                                        gCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTaa                                 >  2:6015074/1‑93 (MQ=255)

                                                        gCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGCCGCGGGAATTaa                                 >  1:4330289/1‑93 (MQ=255)

                                                         cAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAAt                                <  2:5147982/93‑1 (MQ=255)

                                                          aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATa                               >  1:5772124/1‑93 (MQ=255)

                                                          aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATa                               <  2:3056124/93‑1 (MQ=255)

                                                          aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATa                               <  2:6604609/93‑1 (MQ=255)

                                                          aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATa                               <  1:8457840/93‑1 (MQ=255)

                                                            cAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACg                             >  1:6995902/1‑93 (MQ=255)

                                                             aGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGc                            <  2:1771813/93‑1 (MQ=255)

                                                               ccgccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTc                          >  2:3891240/1‑93 (MQ=255)

                                                                cgccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTcc                         <  2:1579359/93‑1 (MQ=255)

                                                                cgccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGACGCGGAAATTAATACGCTcc                         >  1:1906657/1‑93 (MQ=255)

                                                                 gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                        <  1:793628/93‑1 (MQ=255)

                                                                 gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                        >  1:523724/1‑93 (MQ=255)

                                                                  ccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTcccc                       <  1:3674601/93‑1 (MQ=255)

                                                                    tGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCgcgc                     >  1:2607997/1‑93 (MQ=255)

                                                                    tGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                     >  2:5070852/1‑93 (MQ=255)

                                                                      ggATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgccg                   <  1:1440568/93‑1 (MQ=255)

                                                                       gATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCcgccgc                  >  2:643019/1‑93 (MQ=255)

                                                                          gCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCttt               >  2:9459449/1‑93 (MQ=255)

                                                                           cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt              >  1:8450561/1‑93 (MQ=255)

                                                                             ccgTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATCCGCTGCGCGGAAATTAATACGCTCCCCGCCGCTTTTaa            <  2:2941953/91‑1 (MQ=255)

                                                                             cAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTaa            <  1:9348314/93‑1 (MQ=255)

                                                                             cAGTCACTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATGGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTaa            <  1:7685667/93‑1 (MQ=255)

                                                                               gTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAAct          >  2:9733653/1‑93 (MQ=255)

                                                                               gTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAAct          >  2:4254242/1‑93 (MQ=255)

                                                                               gTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAAct          >  2:1139614/1‑93 (MQ=255)

                                                                                tCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActg         >  2:8795365/1‑93 (MQ=255)

                                                                                 cGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActgc        >  2:336674/1‑93 (MQ=255)

                                                                                  gCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActgct       >  1:6300343/1‑93 (MQ=255)

                                                                                   cTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActgctg      >  2:7816066/1‑93 (MQ=255)

                                                                                    tGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGa     >  1:8336651/1‑93 (MQ=255)

                                                                                     gACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGaa    <  2:674941/93‑1 (MQ=255)

                                                                                      aCGCGCCAGAGCAATCAAACAATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAt   <  1:5189206/93‑1 (MQ=255)

TCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATT  >  NC_000913/112148‑112327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`