Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,222,734 T→C intergenic (‑583/‑184) b1172 ← / → b1173 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,222,7340TC99.7% 942.2 / ‑5.2 291intergenic (‑583/‑184)b1172/b1173hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (108/182);  total (109/182)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 316 total aligned reads displayed.

TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG  >  NC_000913/1222644‑1222823
                                                                                          |                                                                                         
ttATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcct                                                                                         <  2:9121225/93‑1 (MQ=255)
ttATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcct                                                                                         >  1:2227560/1‑93 (MQ=255)
   tGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCctggcctggt                                                                                      >  2:3400503/1‑93 (MQ=255)
      gggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGcc                                                                                   <  2:4565404/93‑1 (MQ=255)
      gggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGcc                                                                                   <  2:8554962/93‑1 (MQ=255)
       ggATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGCATCCCCTGGCCTGGTGCCt                                                                                  >  1:1905965/1‑93 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 <  1:8411287/93‑1 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 <  1:5147125/93‑1 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 <  2:5663569/93‑1 (MQ=255)
        gATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTg                                                                                 >  2:1465744/1‑93 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                <  2:3626670/93‑1 (MQ=255)
         atatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGc                                                                                >  1:4307312/1‑93 (MQ=255)
          tatCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCg                                                                               <  1:5882295/93‑1 (MQ=255)
           atcatcATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCgg                                                                              >  1:7328108/1‑93 (MQ=255)
            tcatcaTTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCggg                                                                             >  1:9697857/1‑93 (MQ=255)
              atcatTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCt                                                                           >  1:8329748/1‑93 (MQ=255)
              atcatTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCt                                                                           <  2:6917871/93‑1 (MQ=255)
               tcatTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTg                                                                          >  2:2851462/1‑93 (MQ=255)
                catTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCCGGATGAACCCCCTGGCCTGGTGCCTGCGGGCTGa                                                                         >  1:7215780/1‑93 (MQ=255)
                catTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGa                                                                         <  2:2404411/93‑1 (MQ=255)
                  tttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATg                                                                       <  2:4308458/93‑1 (MQ=255)
                  tttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATg                                                                       >  2:2055011/1‑93 (MQ=255)
                   ttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGt                                                                      <  2:2977931/93‑1 (MQ=255)
                   ttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGt                                                                      <  2:450403/93‑1 (MQ=255)
                   ttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGt                                                                      <  1:5463528/93‑1 (MQ=255)
                   ttCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGt                                                                      <  2:922592/93‑1 (MQ=255)
                    tcatcaTGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGtt                                                                     <  2:6325003/93‑1 (MQ=255)
                     catcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTg                                                                    <  2:6090082/93‑1 (MQ=255)
                     catcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTg                                                                    >  1:8033264/1‑93 (MQ=255)
                     catcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTg                                                                    >  2:7749959/1‑93 (MQ=255)
                      atcatGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgc                                                                   <  1:2957720/93‑1 (MQ=255)
                        catGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcag                                                                 >  2:7581628/1‑93 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               <  2:1083111/93‑1 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               <  1:7421852/93‑1 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               <  1:9479238/93‑1 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               <  1:8706511/93‑1 (MQ=255)
                          tgatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTgcagca                                                               <  1:695968/93‑1 (MQ=255)
                           gatgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGcagcag                                                              <  1:7766680/93‑1 (MQ=255)
                            atgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGcagcagg                                                             >  1:6538467/1‑92 (MQ=255)
                            atgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCaga                                                             >  1:9170822/1‑93 (MQ=255)
                            atgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCaga                                                             <  1:9842385/93‑1 (MQ=255)
                            atgTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCaga                                                             >  1:4900963/1‑93 (MQ=255)
                                cTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCtt                                                         >  1:2412043/1‑93 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        <  2:2247422/93‑1 (MQ=255)
                                 tttGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTa                                                        >  1:608000/1‑93 (MQ=255)
                                   tGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAgg                                                      >  1:7582218/1‑93 (MQ=255)
                                   tGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAgg                                                      <  2:822122/93‑1 (MQ=255)
                                     gTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTc                                                    <  1:448980/93‑1 (MQ=255)
                                     gTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTc                                                    <  1:1537903/93‑1 (MQ=255)
                                       gAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCAc                                                  <  1:9066787/93‑1 (MQ=255)
                                        aGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACt                                                 <  2:7528330/93‑1 (MQ=255)
                                         gCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACtt                                                >  1:3257152/1‑93 (MQ=255)
                                          cGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTa                                               <  2:5477506/93‑1 (MQ=255)
                                          cGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTa                                               <  2:1995870/93‑1 (MQ=255)
                                            gggAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGCGCTTAGGTCACTTAgc                                             >  1:5585813/3‑92 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             <  2:5162658/93‑1 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             <  2:1125425/93‑1 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             <  2:9401438/93‑1 (MQ=255)
                                            gTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGa                                             <  2:5921551/93‑1 (MQ=255)
                                             tGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGAc                                            >  1:3419029/1‑93 (MQ=255)
                                               aaCACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGc                                          <  1:3035336/93‑1 (MQ=255)
                                                acacAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGct                                         <  2:3810712/93‑1 (MQ=255)
                                                  acaATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctac                                       <  2:825569/93‑1 (MQ=255)
                                                    aaTACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGctacta                                     <  2:9831266/93‑1 (MQ=255)
                                                      tACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATc                                   <  2:6204534/93‑1 (MQ=255)
                                                        cacCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAt                                 <  2:3923274/93‑1 (MQ=255)
                                                         acCTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAtt                                >  2:4545890/1‑93 (MQ=255)
                                                          ccTGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCAttt                               >  1:6804827/1‑93 (MQ=255)
                                                            tGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAt                             <  1:1263009/93‑1 (MQ=255)
                                                            tGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAt                             <  2:7341390/93‑1 (MQ=255)
                                                            tGCGCTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTAt                             >  1:4594367/1‑93 (MQ=255)
                                                             gcgcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATc                            >  1:10019753/1‑93 (MQ=255)
                                                             gcgcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATc                            >  1:554388/1‑93 (MQ=255)
                                                              cgcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATcc                           <  1:1841027/93‑1 (MQ=255)
                                                               gcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCa                          >  1:5987976/1‑93 (MQ=255)
                                                               gcTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCa                          >  1:2075344/1‑93 (MQ=255)
                                                                cTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCaa                         <  1:9579330/93‑1 (MQ=255)
                                                                cTGTCTCTTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCaa                         <  2:8919109/93‑1 (MQ=255)
                                                                     tctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCa                    >  1:3528494/1‑93 (MQ=255)
                                                                      ctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCAt                   >  2:7948357/1‑93 (MQ=255)
                                                                      ctTCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCAt                   >  1:6823282/1‑93 (MQ=255)
                                                                       ttCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATg                  <  1:2796246/93‑1 (MQ=255)
                                                                        tCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATgg                 >  1:5397002/1‑93 (MQ=255)
                                                                        tCAGGATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATgg                 <  2:9440767/93‑1 (MQ=255)
                                                                           ggATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAAt              <  1:4036024/93‑1 (MQ=255)
                                                                           ggATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAAt              >  1:3108292/1‑93 (MQ=255)
                                                                            gATGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATc             <  1:3636096/93‑1 (MQ=255)
                                                                              tGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGa           <  1:3673021/93‑1 (MQ=255)
                                                                              tGAATCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGa           >  1:7467757/1‑93 (MQ=255)
                                                                                aaTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaaa         >  2:183288/1‑93 (MQ=255)
                                                                                aaTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGaaa         <  2:4105601/93‑1 (MQ=255)
                                                                                 aTCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGATCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAAt        <  2:9656907/93‑1 (MQ=255)
                                                                                  tCCCCTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATc       <  2:8480225/93‑1 (MQ=255)
                                                                                   ccccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGGAATCa      <  1:5732519/93‑1 (MQ=255)
                                                                                    cccTGGCCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAg     <  1:194511/93‑1 (MQ=255)
                                                                                     cctggcctggTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGt    <  2:8998713/93‑1 (MQ=255)
                                                                                      ctggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTc   <  1:5829047/93‑1 (MQ=255)
                                                                                      ctggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTc   <  2:6515149/93‑1 (MQ=255)
                                                                                       tggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCg  >  1:2575270/1‑93 (MQ=255)
                                                                                       tggcctggtGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCg  >  2:7095472/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTATGCGGGATATCATCATTTCATCATGATGTCTTTGGTGAGCGGTGAACACAATACACCTGCGCTGTCTCTTCAGGATGAATCCCCTGGTCTGGTGCCTGCGGGCTGATGTTGCAGCAGAGCTTAGGTCACTTAGACGCTACTATCATTTATCCAATGGCATGGAATCGAAATCAGTCG  >  NC_000913/1222644‑1222823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑