BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,226,344	C→T	A118T (GCC→ACC)	ycgK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,344	0	C	T	99.3%	1070.6 / NA	304	A118T (GCC→ACC)	ycgK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (153/148); minor base A (1/1); total (155/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

ATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATC  >  NC_000913/1226254‑1226433

aTTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGgtgt                                                                                         >  1:8590756/1‑93 (MQ=255)

 ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtc                                                                                        >  2:7587399/1‑93 (MQ=255)

 ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtc                                                                                        >  2:962922/1‑93 (MQ=255)

 ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtc                                                                                        >  1:3620195/1‑93 (MQ=255)

  tAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtcg                                                                                       <  2:3116250/93‑1 (MQ=255)

  tAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGtgtcg                                                                                       >  2:3412646/1‑93 (MQ=255)

     cGTTTATGACGGGGAGATTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGGTTTGTTTTTACGGTtgtcgggt                                                                                    >  2:5642971/1‑90 (MQ=255)

       tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCg                                                                                  >  2:8127311/1‑93 (MQ=255)

       tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCg                                                                                  >  2:5071501/1‑93 (MQ=255)

        ttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 <  1:178137/93‑1 (MQ=255)

           tGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGtt                                                                              <  1:2949943/93‑1 (MQ=255)

               ggggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGGTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCa                                                                          >  1:8337579/1‑93 (MQ=255)

                gggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCaa                                                                         >  1:2480820/1‑93 (MQ=255)

                 ggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAac                                                                        <  1:9266513/93‑1 (MQ=255)

                 ggAGAGGCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAac                                                                        <  1:6929045/93‑1 (MQ=255)

                    gagTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACt                                                                     >  1:4222942/1‑93 (MQ=255)

                    gagTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACt                                                                     >  1:7577774/1‑93 (MQ=255)

                     agTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTc                                                                    <  1:5628847/93‑1 (MQ=255)

                      gTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCg                                                                   >  1:1647257/1‑93 (MQ=255)

                       tCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGc                                                                  >  2:5703379/1‑93 (MQ=255)

                           cGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCt                                                              >  1:1896934/1‑93 (MQ=255)

                           cGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCt                                                              <  1:4792265/93‑1 (MQ=255)

                            gTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTc                                                             >  1:1594189/1‑93 (MQ=255)

                            gTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTc                                                             >  1:8393619/1‑93 (MQ=255)

                               ggTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCata                                                          >  1:9383145/1‑93 (MQ=255)

                               ggTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCata                                                          <  1:5526908/93‑1 (MQ=255)

                               ggTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCata                                                          >  1:680955/1‑93 (MQ=255)

                                gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         <  2:8611921/93‑1 (MQ=255)

                                gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         >  2:7362651/1‑93 (MQ=255)

                                gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         >  1:6981838/1‑93 (MQ=255)

                                 ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        <  2:4378944/93‑1 (MQ=255)

                                 ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        <  1:6304264/93‑1 (MQ=255)

                                  tGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAttt                                                       >  2:1380071/1‑93 (MQ=255)

                                   gACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTg                                                      >  1:3658912/1‑93 (MQ=255)

                                   gACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTg                                                      >  1:7687959/1‑93 (MQ=255)

                                      aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   <  2:60720/93‑1 (MQ=255)

                                      aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   >  2:7852584/1‑93 (MQ=255)

                                      aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTGGCAACACTCGCAGCTCATATTTGCCa                                                   <  1:1512397/93‑1 (MQ=255)

                                      aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGAGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   >  1:7935068/1‑93 (MQ=255)

                                       tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  2:91009/1‑93 (MQ=255)

                                       tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  2:3702114/1‑93 (MQ=255)

                                       tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  1:4366689/1‑93 (MQ=255)

                                       tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  1:3116326/1‑93 (MQ=255)

                                        ttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGa                                                 >  1:10008023/1‑93 (MQ=255)

                                         tACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGaa                                                <  2:9415174/93‑1 (MQ=255)

                                            ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             <  2:3621922/93‑1 (MQ=255)

                                            ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             <  1:467114/93‑1 (MQ=255)

                                             tAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAg                                            >  2:5351198/1‑93 (MQ=255)

                                             tAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAg                                            <  2:2830468/93‑1 (MQ=255)

                                                tttGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGga                                         >  1:2318008/1‑93 (MQ=255)

                                                tttGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGga                                         >  1:6454/1‑93 (MQ=255)

                                                 ttGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgag                                        >  2:8230284/1‑93 (MQ=255)

                                                 ttGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgag                                        <  2:2035855/93‑1 (MQ=255)

                                                  tGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagg                                       >  2:3229558/1‑92 (MQ=255)

                                                   gAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtg                                      <  1:8849832/93‑1 (MQ=255)

                                                    aaTATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtga                                     <  2:7658518/93‑1 (MQ=255)

                                                    aaTATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagcga                                     >  2:1102358/1‑93 (MQ=255)

                                                     atatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgag                                    <  2:7102700/93‑1 (MQ=255)

                                                     atatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgag                                    <  2:6711565/93‑1 (MQ=255)

                                                      tatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgagg                                   >  1:9567051/1‑92 (MQ=255)

                                                      tatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgaga                                   >  1:4560528/1‑92 (MQ=255)

                                                      tatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTATGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgaga                                   >  1:5354767/1‑92 (MQ=255)

                                                       atCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTa                                  >  2:9116618/1‑93 (MQ=255)

                                                       atCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTa                                  >  1:1265486/1‑93 (MQ=255)

                                                       atCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGCGAGTGAGTa                                  >  2:1915911/1‑93 (MQ=255)

                                                        tCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAt                                 <  2:1820604/93‑1 (MQ=255)

                                                         cGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAtt                                >  1:7161512/1‑93 (MQ=255)

                                                         cGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAtt                                <  1:5269227/93‑1 (MQ=255)

                                                          gACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTg                               <  1:8242098/93‑1 (MQ=255)

                                                           aCGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGt                              >  1:7435365/1‑93 (MQ=255)

                                                            cGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTc                             >  1:8099983/1‑93 (MQ=255)

                                                            cGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTc                             <  1:3922708/93‑1 (MQ=255)

                                                             gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            <  2:1713650/93‑1 (MQ=255)

                                                             gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            <  1:989836/93‑1 (MQ=255)

                                                                 tatTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGa                        <  2:6023220/93‑1 (MQ=255)

                                                                  atTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGAc                       <  2:9287264/93‑1 (MQ=255)

                                                                  atTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGAc                       <  1:9699613/93‑1 (MQ=255)

                                                                  atTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGAAACAATCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTACGTGAc                       >  2:4624272/1‑93 (MQ=255)

                                                                    tttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTa                     >  1:3794294/1‑93 (MQ=255)

                                                                     ttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTAt                    >  1:2640226/1‑93 (MQ=255)

                                                                     ttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTAt                    <  1:6093257/93‑1 (MQ=255)

                                                                      tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATc                   >  1:2108008/1‑93 (MQ=255)

                                                                      tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGCATTGTCCGTGACTATc                   >  2:8441721/1‑93 (MQ=255)

                                                                           ttttgttttTACGGGTGTCGTTTCGGGCTTGCCACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGAGAGGATTGTCCGTGACTATCGAGtt              >  2:6624144/1‑93 (MQ=255)

                                                                            tttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTc             <  1:5099878/93‑1 (MQ=255)

                                                                             ttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCt            >  1:113996/1‑93 (MQ=255)

                                                                             ttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCt            >  2:5503163/1‑93 (MQ=255)

                                                                             ttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCt            <  2:2622795/93‑1 (MQ=255)

                                                                             ttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCt            <  1:1341235/93‑1 (MQ=255)

                                                                              tgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTg           <  2:9007870/93‑1 (MQ=255)

                                                                               gttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTgg          <  1:2217332/93‑1 (MQ=255)

                                                                                tttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTggg         <  2:4713108/93‑1 (MQ=255)

                                                                                tttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTggg         <  2:4093675/93‑1 (MQ=255)

                                                                                 ttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTgggg        <  1:443144/93‑1 (MQ=255)

                                                                                  tttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGa       >  1:3265138/1‑93 (MQ=255)

                                                                                   ttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGaa      <  2:829338/93‑1 (MQ=255)

                                                                                   ttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGaa      >  2:9743964/1‑93 (MQ=255)

                                                                                     aCGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAata    <  2:2279424/93‑1 (MQ=255)

                                                                                       gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  <  2:8816381/93‑1 (MQ=255)

                                                                                       gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  >  1:5848346/1‑93 (MQ=255)

ATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATC  >  NC_000913/1226254‑1226433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`