BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,972	A→G	G188G (GGA→GGG)	umuC →	UmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,972	0	A	G	99.6%	841.8 / ‑5.2	263	G188G (GGA→GGG)	umuC	UmuC
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (147/115); total (148/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 277 total aligned reads displayed.

GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT  >  NC_000913/1230882‑1231061

  aGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGggcggc                                                                                       >  2:1022735/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  2:9890554/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  2:8876221/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  2:509397/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      <  2:1277303/93‑1 (MQ=255)

     ggggtggggtgGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGa                                                                                    <  2:57274/92‑1 (MQ=255)

       ggtggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATc                                                                                  >  2:8302783/1‑93 (MQ=255)

          ggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGc                                                                               <  2:6073942/93‑1 (MQ=255)

          ggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGAGGATCAGc                                                                               >  1:386175/1‑93 (MQ=255)

              tggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaa                                                                           >  1:5168317/1‑93 (MQ=255)

               ggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaa                                                                          >  1:6655351/1‑93 (MQ=255)

                gtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaaa                                                                         >  1:7665333/1‑93 (MQ=255)

                gtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaaa                                                                         >  1:4341403/1‑93 (MQ=255)

                gtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaaa                                                                         >  1:319087/1‑93 (MQ=255)

                 tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGATGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAc                                                                        <  1:1261648/93‑1 (MQ=255)

                 tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAc                                                                        <  1:6255379/93‑1 (MQ=255)

                  ggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACt                                                                       >  2:9400039/1‑93 (MQ=255)

                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  2:2791816/93‑1 (MQ=255)

                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  1:5651792/93‑1 (MQ=255)

                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  1:9203781/93‑1 (MQ=255)

                    aTTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTgg                                                                     <  1:9884966/93‑1 (MQ=255)

                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    <  1:165199/93‑1 (MQ=255)

                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    <  1:8892730/93‑1 (MQ=255)

                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    >  1:5288490/1‑93 (MQ=255)

                       tttCAAATCTGGAACGCCAGCGGAAATTAATGTCTGCTCTCCCCGTGGGTGACGTCTGGGGGATTTGGCGGCCGATCAGCAAAAAACTGGagg                                                                  >  2:6141232/3‑91 (MQ=255)

                       tATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcg                                                                  >  1:1013599/1‑93 (MQ=255)

                        aTCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcgc                                                                 <  2:8508212/93‑1 (MQ=255)

                          cAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGa                                                               <  1:8298242/93‑1 (MQ=255)

                          cAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGa                                                               >  2:7691743/1‑93 (MQ=255)

                          cAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGa                                                               <  1:9358034/93‑1 (MQ=255)

                           aaaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAt                                                              >  1:4132067/1‑93 (MQ=255)

                            aaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATg                                                             >  2:9161707/1‑93 (MQ=255)

                            aaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATg                                                             <  2:5368112/93‑1 (MQ=255)

                               cTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATgggg                                                          >  1:5085299/1‑93 (MQ=255)

                                 ggAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAt                                                        <  1:5769921/93‑1 (MQ=255)

                                  gAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATc                                                       >  1:6769624/1‑93 (MQ=255)

                                   aaCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCa                                                      <  2:9516277/93‑1 (MQ=255)

                                   aaCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCa                                                      >  2:7072939/1‑93 (MQ=255)

                                    aCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaa                                                     >  1:2016231/1‑93 (MQ=255)

                                     cGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATTACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaa                                                    >  1:2763210/1‑93 (MQ=255)

                                     cGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaa                                                    <  1:8170844/93‑1 (MQ=255)

                                     cGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaa                                                    <  1:6187436/93‑1 (MQ=255)

                                      gCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaaa                                                   >  1:2505267/1‑93 (MQ=255)

                                        cAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAAcc                                                 >  1:1418840/1‑93 (MQ=255)

                                          gCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGt                                               >  1:6600576/1‑93 (MQ=255)

                                           cGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGtt                                              <  1:2553080/93‑1 (MQ=255)

                                           cGTAAATTAATAGCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGGCGCGATGGGGATCAAAACCGtt                                              <  2:4147048/93‑1 (MQ=255)

                                            gTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtc                                             <  2:5547243/93‑1 (MQ=255)

                                              aaaTTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtctc                                           >  1:2843785/1‑93 (MQ=255)

                                               aaTTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCg                                          >  2:4408065/1‑93 (MQ=255)

                                                  tAATGGCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAtt                                       <  1:8556071/93‑1 (MQ=255)

                                                   aaTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAttt                                      >  2:3462848/1‑93 (MQ=255)

                                                    aTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTg                                     >  1:9210769/1‑93 (MQ=255)

                                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTgg                                    >  1:1731012/1‑93 (MQ=255)

                                                      gTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGc                                   <  2:6240798/93‑1 (MQ=255)

                                                      gTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGc                                   >  1:4667219/1‑93 (MQ=255)

                                                       tCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCg                                  >  2:3826926/1‑93 (MQ=255)

                                                        cTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCgg                                 >  1:6432937/1‑93 (MQ=255)

                                                        cTGCTCTCCCAGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGTATCAAAACCGTCCTCGATTTGGCgg                                 <  2:6511397/93‑1 (MQ=255)

                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTggagga                                >  2:1587100/1‑93 (MQ=255)

                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGa                                <  2:6593940/93‑1 (MQ=255)

                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGa                                <  2:3197928/93‑1 (MQ=255)

                                                           ctctCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATa                              >  1:8017101/1‑93 (MQ=255)

                                                            tctcCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATAc                             <  2:6720148/93‑1 (MQ=255)

                                                               ccccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGa                          >  2:7644832/1‑93 (MQ=255)

                                                               ccccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGa                          >  2:4703674/1‑93 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         <  2:4306243/93‑1 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         >  1:3369093/1‑93 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         <  1:8131984/93‑1 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         >  2:9777596/1‑93 (MQ=255)

                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        <  2:3008622/93‑1 (MQ=255)

                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        <  1:7292231/93‑1 (MQ=255)

                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        >  1:3976038/1‑93 (MQ=255)

                                                                  cGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGTTACAGatat                       >  1:9042226/1‑93 (MQ=255)

                                                                  cGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTAGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGatat                       >  2:2873983/1‑93 (MQ=255)

                                                                   gTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATc                      >  2:7771613/1‑93 (MQ=255)

                                                                   gTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATc                      <  1:7813395/93‑1 (MQ=255)

                                                                    tGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATcc                     >  1:3686518/1‑93 (MQ=255)

                                                                    tGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATcc                     >  2:3662945/1‑93 (MQ=255)

                                                                      gATGCCGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCgg                   <  2:2219829/93‑1 (MQ=255)

                                                                      gATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCgg                   >  2:1640583/1‑93 (MQ=255)

                                                                       aTGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGt                  <  1:3004758/93‑1 (MQ=255)

                                                                        tGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGAACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGtt                 >  2:1710232/1‑93 (MQ=255)

                                                                          aCGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTa               <  2:9564752/93‑1 (MQ=255)

                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:7242472/93‑1 (MQ=255)

                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:1385517/93‑1 (MQ=255)

                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:2112113/93‑1 (MQ=255)

                                                                             tcggggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            <  2:7373605/90‑1 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATCCAGATATCCGGTTTATCTGt          >  1:7521882/1‑93 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          >  2:8112721/1‑93 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          >  1:8781893/1‑93 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          <  2:309803/93‑1 (MQ=255)

                                                                                gggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTa         <  1:4630885/93‑1 (MQ=255)

                                                                                 ggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTaa        >  2:5191844/1‑93 (MQ=255)

                                                                                   ggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAAc      >  2:2327681/1‑93 (MQ=255)

                                                                                    gATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACa     >  2:4070844/1‑93 (MQ=255)

                                                                                    gATTGGGCCGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACa     <  2:8651576/93‑1 (MQ=255)

                                                                                     aTTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAATCCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAt    <  1:2282251/93‑1 (MQ=255)

                                                                                      ttGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAtt   >  2:5929888/1‑93 (MQ=255)

                                                                                       tGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAttt  <  2:2031118/93‑1 (MQ=255)

GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT  >  NC_000913/1230882‑1231061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`