BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,788	A→G	C438R (TGC→CGC)	hofB ←	HofB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,788	0	A	G	99.7%	904.9 / ‑5.0	285	C438R (TGC→CGC)	hofB	HofB
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (176/108); total (176/109)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

Only 100 of 331 total aligned reads displayed.

CCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAA  >  NC_000913/115698‑115877

 caGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGcc                                                                                        <  1:8437785/93‑1 (MQ=255)

  aGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCg                                                                                       >  2:5479476/1‑93 (MQ=255)

   gcgcCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGAGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGt                                                                                      >  1:4699178/1‑93 (MQ=255)

     gcCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGttt                                                                                    >  1:3906022/1‑93 (MQ=255)

     gcCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGttt                                                                                    <  1:9477197/93‑1 (MQ=255)

        agagTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCa                                                                                 >  1:8930642/1‑93 (MQ=255)

         gagTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCaa                                                                                >  1:1207988/1‑93 (MQ=255)

         gagTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCaa                                                                                >  1:3459305/1‑93 (MQ=255)

           gTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCaaaa                                                                              >  2:5847534/1‑93 (MQ=255)

            ttGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCaaaaa                                                                             <  2:530889/93‑1 (MQ=255)

             tGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAg                                                                            >  1:3680747/1‑93 (MQ=255)

              gCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGc                                                                           >  2:9746981/1‑93 (MQ=255)

              gCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGc                                                                           >  1:5155968/1‑93 (MQ=255)

                ttACTCGCCATGCGGCATCCCCAGTACGCGGATTAGCTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGGCGTTTTCAAAAAGCGt                                                                         >  2:1176773/1‑93 (MQ=255)

                ttACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGt                                                                         <  1:2573297/93‑1 (MQ=255)

                 tACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTa                                                                        <  2:8164823/93‑1 (MQ=255)

                 tACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTa                                                                        <  2:1951766/93‑1 (MQ=255)

                 tACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTa                                                                        >  1:4997335/1‑93 (MQ=255)

                 tACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTa                                                                        <  2:2265434/93‑1 (MQ=255)

                   cTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACg                                                                      >  2:2976506/1‑93 (MQ=255)

                    tCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCCAAAAGCGTACGc                                                                     >  2:9121990/1‑93 (MQ=255)

                    tCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGc                                                                     <  2:8916014/93‑1 (MQ=255)

                     cGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCa                                                                    <  2:202890/93‑1 (MQ=255)

                     cGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCa                                                                    <  1:7528907/93‑1 (MQ=255)

                     cGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCa                                                                    <  2:8775220/93‑1 (MQ=255)

                       ccATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATa                                                                  <  2:3521430/93‑1 (MQ=255)

                        cATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATAc                                                                 >  2:5638597/1‑93 (MQ=255)

                         aTGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATAcc                                                                >  2:3133410/1‑93 (MQ=255)

                         aTGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATAcc                                                                <  1:712273/93‑1 (MQ=255)

                          tGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATAccc                                                               <  1:4468116/93‑1 (MQ=255)

                           gCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCg                                                              <  2:3840799/93‑1 (MQ=255)

                            cGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGc                                                             <  2:1855015/93‑1 (MQ=255)

                             ggCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGcc                                                            <  2:6440872/93‑1 (MQ=255)

                             ggCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGcc                                                            <  2:9862691/93‑1 (MQ=255)

                                aTCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGt                                                         >  2:8111845/1‑93 (MQ=255)

                                 tCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTc                                                        >  2:7834594/1‑93 (MQ=255)

                                 tCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTc                                                        >  2:4659144/1‑93 (MQ=255)

                                        tACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATCCCCGCCTGTcgtgcgtg                                                 <  1:8311494/93‑1 (MQ=255)

                                        tACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTcgtgcgtg                                                 >  1:9916372/1‑93 (MQ=255)

                                        tACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTcgtgcgtg                                                 >  1:9182796/1‑93 (MQ=255)

                                            cgGATTAACTCTTCAAAGGTGTTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGtt                                             <  2:4402903/93‑1 (MQ=255)

                                            cgGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGtt                                             <  2:276235/93‑1 (MQ=255)

                                             ggATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAACAAGCGTACGCATACCCGCCTGTCGTGCGTGCGttt                                            >  1:6106094/1‑93 (MQ=255)

                                             ggATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGttt                                            <  1:4267664/93‑1 (MQ=255)

                                             ggATTAACTCTTCAAAGGTGGTTAAGACTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGCACGCATACCAGCCTGTCGTGCGTGCGttt                                            >  2:4098974/1‑93 (MQ=255)

                                              gATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTc                                           >  1:6186733/1‑93 (MQ=255)

                                               aTTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTcc                                          >  2:6902109/1‑93 (MQ=255)

                                                 tAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTCCGCATCCCCGCCTGTCGTGCGTGCGTTTCCAg                                        <  1:5505179/93‑1 (MQ=255)

                                                 tAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAg                                        >  1:6046191/1‑93 (MQ=255)

                                                 tAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAg                                        >  2:6705470/1‑93 (MQ=255)

                                                 tAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAg                                        >  1:1064629/1‑93 (MQ=255)

                                                 tAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAg                                        <  1:10045867/93‑1 (MQ=255)

                                                  aaCTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGc                                       >  1:7326646/1‑93 (MQ=255)

                                                  aaCTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGc                                       >  1:196753/1‑93 (MQ=255)

                                                   aCTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCg                                      >  1:1433570/1‑93 (MQ=255)

                                                   aCTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGACCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCg                                      >  2:638197/1‑93 (MQ=255)

                                                   aCTCTCCAAAGGTGGTTAAGCCTTGCTGTACGTCCAGGCGCCCGTTTTCAAAAAGCGAACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCg                                      <  2:2913550/93‑1 (MQ=255)

                                                     tctTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGAt                                    >  1:6353604/1‑93 (MQ=255)

                                                      ctTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAACGTACGCATACCCGCCTGTCGTGCGTGAGTTTACAGCGAtt                                   >  1:7662340/1‑93 (MQ=255)

                                                         cAAGGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCaa                                <  1:565992/93‑1 (MQ=255)

                                                         cAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCaa                                >  2:4804020/1‑93 (MQ=255)

                                                         cAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCaa                                <  1:3007144/93‑1 (MQ=255)

                                                          aaaGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAAc                               <  2:1623168/93‑1 (MQ=255)

                                                           agggtgttTAAGCCTTGCTCTACGGCCAGGCAGCCGTTTTCAAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACg                              <  2:3665942/91‑1 (MQ=255)

                                                           aaGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACg                              >  1:8776105/1‑93 (MQ=255)

                                                           aaGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACg                              <  1:272068/93‑1 (MQ=255)

                                                            aGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGt                             >  1:1845475/1‑93 (MQ=255)

                                                            aGGTGGTTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGt                             >  1:8662653/1‑93 (MQ=255)

                                                             ggtggtTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTc                            <  2:752748/93‑1 (MQ=255)

                                                                 gtTACGCCTTCCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCTTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTa                        <  2:1516853/93‑1 (MQ=255)

                                                                 gtTAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTa                        >  1:2336695/1‑93 (MQ=255)

                                                                  ttAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTAt                       >  1:4515798/1‑93 (MQ=255)

                                                                  ttAAGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTAt                       <  2:3426670/93‑1 (MQ=255)

                                                                   tAAGCTTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTAtt                      <  2:3994660/93‑1 (MQ=255)

                                                                    aaGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTa                     >  2:6173481/1‑93 (MQ=255)

                                                                     aGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAg                    >  2:3952891/1‑93 (MQ=255)

                                                                     aGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAg                    >  1:7044265/1‑93 (MQ=255)

                                                                     aGCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAg                    >  2:8623840/1‑93 (MQ=255)

                                                                      gCCTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGc                   >  2:4641063/1‑93 (MQ=255)

                                                                       ccTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCg                  >  1:506840/1‑93 (MQ=255)

                                                                        cTTGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCgg                 >  2:1076172/1‑93 (MQ=255)

                                                                         ttGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCCGCGATTCAACGTCGGTATTAGCGGa                >  2:6852992/1‑93 (MQ=255)

                                                                         ttGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGa                >  1:125100/1‑93 (MQ=255)

                                                                          tGCTCTACGGCCAGGCGGCCTTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGaa               <  1:4047712/93‑1 (MQ=255)

                                                                          tGCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGaa               >  2:5363170/1‑93 (MQ=255)

                                                                           gCTCTACGGCCAGGCGGCTGTTTTCAAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGaaa              <  2:82678/93‑1 (MQ=255)

                                                                           gCTCTACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGaaa              <  2:9340946/93‑1 (MQ=255)

                                                                             tctcCGGCCAGCGGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATa            <  2:100416/93‑1 (MQ=255)

                                                                             tctGCGGCGAGGCGGCCGTTTTATAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATa            <  2:5530075/93‑1 (MQ=255)

                                                                             tctACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATa            <  1:3064136/93‑1 (MQ=255)

                                                                             tctACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATa            <  2:1326190/93‑1 (MQ=255)

                                                                              ctACGGCCAGGCGGCCGTTTTCAAAAAGCGTACTCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATaa           >  1:9772936/1‑93 (MQ=255)

                                                                               tACGGCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAg          >  2:5198975/1‑93 (MQ=255)

                                                                                   gcaaGGCGGCAGTTTTCAAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGa      <  2:9241328/90‑1 (MQ=255)

                                                                                   gCCAGGCGGCCGTTTTCAAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGa      <  1:5818411/93‑1 (MQ=255)

                                                                                   gCCAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGa      >  2:7347922/1‑93 (MQ=255)

                                                                                    ccAGGCGGCCGTTTTCAAAACGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGAc     <  2:9410291/93‑1 (MQ=255)

                                                                                     cAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACg    >  2:2255142/1‑93 (MQ=255)

                                                                                     cAGGCGGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGGATTAGCGGAAATAAGCTGACg    >  2:7117288/1‑93 (MQ=255)

                                                                                       ggcggcCGTTTTCAAAAAGCGTACGCATCCCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGaa  <  2:4313062/93‑1 (MQ=255)

CCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGCCGTTTTCAAAAAGCGTACGCATACCCGCCTGTCGTGCGTGCGTTTCCAGCGATTCAACGTCGGTATTAGCGGAAATAAGCTGACGAA  >  NC_000913/115698‑115877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`