BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,543	T→C	F27L (TTT→CTT)	hnr →	Hnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,543	0	T	C	99.6%	834.3 / NA	259	F27L (TTT→CTT)	hnr	Hnr
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (138/120); total (138/121)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  1:7244673/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  2:6344038/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  2:6288916/1‑93 (MQ=255)

   ggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTcc                                                                                      <  1:2233106/93‑1 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGTATTTATGGCTTtcatca                                                                                   >  1:6108907/1‑93 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                   <  2:3554818/93‑1 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                   >  1:4589558/1‑93 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                   <  2:1780534/93‑1 (MQ=255)

        gaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAtt                                                                                 <  2:9101261/93‑1 (MQ=255)

         aaCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTg                                                                                <  2:8558595/93‑1 (MQ=255)

          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                               <  2:1987455/93‑1 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                              >  2:4087768/1‑93 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                              <  2:9673399/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgggtg                                                                            >  2:7712144/1‑91 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                            <  2:1302279/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                            <  1:328237/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                            <  1:9374006/93‑1 (MQ=255)

              gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGc                                                                           <  2:5054417/93‑1 (MQ=255)

                cGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGa                                                                         <  1:6078633/93‑1 (MQ=255)

                  cAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACa                                                                       >  1:1006598/1‑93 (MQ=255)

                  cAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACa                                                                       >  2:1172242/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      <  1:259453/93‑1 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      >  1:8559925/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      <  1:5989922/93‑1 (MQ=255)

                    gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAc                                                                     <  1:8372168/93‑1 (MQ=255)

                    gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAc                                                                     >  1:1386562/1‑93 (MQ=255)

                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   >  2:7287200/1‑93 (MQ=255)

                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   >  1:1409572/1‑93 (MQ=255)

                      cATGGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   <  2:2093086/93‑1 (MQ=255)

                       aTTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGt                                                                  >  1:1611833/1‑93 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  1:2812407/93‑1 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  1:3965191/93‑1 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                >  2:4097275/1‑93 (MQ=255)

                          ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACt                                                               <  2:4400562/93‑1 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              >  2:2447021/1‑93 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              <  2:2588199/93‑1 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              >  1:1186534/1‑93 (MQ=255)

                            gcGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTgg                                                             <  2:7521041/92‑1 (MQ=255)

                             cGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggc                                                            <  2:3047340/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         <  1:5293113/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         >  1:739179/1‑93 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        >  2:8812315/1‑93 (MQ=255)

                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                       >  2:1472808/1‑93 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      <  1:2463905/93‑1 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      <  1:2335412/93‑1 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      >  1:10009240/1‑93 (MQ=255)

                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                     <  2:477569/93‑1 (MQ=255)

                                     aGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATg                                                    >  2:3989455/1‑93 (MQ=255)

                                     aGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATg                                                    >  1:691859/1‑93 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                   >  1:6750758/1‑93 (MQ=255)

                                       aTTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggg                                                  <  1:2131897/93‑1 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  1:2518063/93‑1 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  1:3587372/93‑1 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggggg                                                >  2:6444617/1‑92 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt                                                <  2:9484957/93‑1 (MQ=255)

                                            cATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa                                             <  1:5683123/93‑1 (MQ=255)

                                            cATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa                                             <  1:6004417/93‑1 (MQ=255)

                                             aTTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAt                                            <  2:5320902/93‑1 (MQ=255)

                                               tgttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGc                                          >  1:8327069/1‑93 (MQ=255)

                                                gttgAAGATGAGCAGGGATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCGGACGGGGTGGATGcc                                         >  2:8957113/1‑93 (MQ=255)

                                                 ttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGccc                                        <  1:7409256/93‑1 (MQ=255)

                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       >  2:1551325/1‑93 (MQ=255)

                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       <  2:2972039/93‑1 (MQ=255)

                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       <  1:6268706/93‑1 (MQ=255)

                                                   gAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCtt                                      >  2:5664805/1‑93 (MQ=255)

                                                   gAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCtt                                      >  1:9206067/1‑93 (MQ=255)

                                                    aaGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTg                                     >  1:288130/1‑93 (MQ=255)

                                                     aGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGa                                    >  2:4368526/1‑93 (MQ=255)

                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                 >  2:3955428/1‑93 (MQ=255)

                                                         gAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTg                                >  2:9443421/1‑93 (MQ=255)

                                                         gAGCAGGTATTTCGCTCGCTGCTGGATTCATGGCTGTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGGCCTTGAGTTg                                <  2:5222159/93‑1 (MQ=255)

                                                            cAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTg                             >  1:4234261/1‑93 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            <  2:3363594/93‑1 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            >  2:7336890/1‑93 (MQ=255)

                                                              ggTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTggg                           >  2:6042607/1‑93 (MQ=255)

                                                               gTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgggg                          >  2:323475/1‑92 (MQ=255)

                                                               gTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGa                          <  2:9751224/93‑1 (MQ=255)

                                                                tATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAg                         >  2:3290162/1‑93 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAgg                        >  1:9030770/1‑93 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       <  2:3788243/93‑1 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       >  1:7003086/1‑93 (MQ=255)

                                                                   ttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGtt                      <  2:4745776/93‑1 (MQ=255)

                                                                     cgctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTc                    >  1:4710956/1‑93 (MQ=255)

                                                                     cgctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTc                    <  1:5081332/93‑1 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                  >  2:5368219/1‑93 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                  >  1:5612142/1‑93 (MQ=255)

                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACt                 <  2:8195761/93‑1 (MQ=255)

                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTcc               >  1:3363402/1‑93 (MQ=255)

                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTcc               >  1:7136419/1‑93 (MQ=255)

                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              <  1:4662354/93‑1 (MQ=255)

                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              >  2:8315971/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            >  2:8798673/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            >  2:6775720/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            >  2:6035011/1‑93 (MQ=255)

                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCAGACCt         >  1:6433256/1‑93 (MQ=255)

                                                                                  aTTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGGTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGGTTTCACTACAGACCtgt       >  1:8082965/1‑92 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      <  1:9967985/93‑1 (MQ=255)

                                                                                     cATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgatga    <  1:411594/93‑1 (MQ=255)

                                                                                      aTGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTgatgat   <  2:6312491/93‑1 (MQ=255)

                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGata  <  1:5652142/93‑1 (MQ=255)

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`