BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,293,354	A→G	H202R (CAT→CGT)	tdk →	thymidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,293,354	0	A	G	99.7%	1145.8 / NA	357	H202R (CAT→CGT)	tdk	thymidine kinase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (171/184); total (171/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

TTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTA  >  NC_000913/1293264‑1293443

ttGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTc                                                                                         >  2:6905599/1‑93 (MQ=255)

  ggtggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGc                                                                                       >  1:6273519/1‑93 (MQ=255)

  ggtggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGc                                                                                       >  2:7166269/1‑93 (MQ=255)

  ggtggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGc                                                                                       <  1:3307781/93‑1 (MQ=255)

   gtggtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGcc                                                                                      <  2:7677379/93‑1 (MQ=255)

      gtAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACg                                                                                   >  1:2177207/1‑93 (MQ=255)

       tAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGa                                                                                  <  2:2545211/93‑1 (MQ=255)

       tAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGa                                                                                  >  2:539254/1‑93 (MQ=255)

        aaTGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGAt                                                                                 >  1:4825547/1‑93 (MQ=255)

          tGAACGATACGTTTCTGTATGCCGTACACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATta                                                                               >  1:125/1‑93 (MQ=255)

          tGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATta                                                                               >  1:6454312/1‑93 (MQ=255)

          tGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATta                                                                               <  2:918689/93‑1 (MQ=255)

            aaCGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaat                                                                             <  2:8044530/93‑1 (MQ=255)

            aaCGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaat                                                                             >  2:6413851/1‑93 (MQ=255)

            aaCGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaat                                                                             <  1:4673363/93‑1 (MQ=255)

              cGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATtaataa                                                                           >  1:8206456/1‑93 (MQ=255)

               gATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAg                                                                          <  1:2972210/93‑1 (MQ=255)

               gATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAg                                                                          <  1:9913034/93‑1 (MQ=255)

                 tACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGaa                                                                        >  1:9173019/1‑93 (MQ=255)

                  aCGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAt                                                                       >  1:3397803/1‑93 (MQ=255)

                   cGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAAtt                                                                      <  2:4154375/93‑1 (MQ=255)

                     tttCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTc                                                                    <  1:4336269/93‑1 (MQ=255)

                     tttCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTc                                                                    >  2:4944303/1‑93 (MQ=255)

                       tCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCtt                                                                  >  2:7547984/1‑93 (MQ=255)

                       tCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCtt                                                                  <  1:8105970/93‑1 (MQ=255)

                       tCTGCATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGGCTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCtt                                                                  >  2:5653941/1‑93 (MQ=255)

                        cTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCttt                                                                 >  2:794516/1‑93 (MQ=255)

                          gTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTAc                                                               >  2:7013053/1‑93 (MQ=255)

                          gTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTAc                                                               >  2:1911927/1‑93 (MQ=255)

                          gTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTAc                                                               >  2:4008069/1‑93 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            <  1:952973/93‑1 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            >  1:8154737/1‑93 (MQ=255)

                             tGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGa                                                            <  1:2283606/93‑1 (MQ=255)

                              gCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGAc                                                           <  2:9407469/93‑1 (MQ=255)

                               ccGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACa                                                          <  1:7632760/93‑1 (MQ=255)

                                cGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAg                                                         <  2:6289420/93‑1 (MQ=255)

                                 gTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAgg                                                        >  2:3239871/1‑93 (MQ=255)

                                  tAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAggg                                                       >  1:8299501/1‑93 (MQ=255)

                                  tAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAggg                                                       <  1:2057965/93‑1 (MQ=255)

                                  tAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAggg                                                       >  2:5804835/1‑93 (MQ=255)

                                  gaaaCACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAggg                                                       <  1:1452096/92‑1 (MQ=255)

                                   aaaCACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAgggg                                                      >  1:1034719/1‑92 (MQ=255)

                                   aaaCACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGt                                                      <  1:419165/93‑1 (MQ=255)

                                    aaCACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTg                                                     <  1:5473136/93‑1 (MQ=255)

                                     acacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGa                                                    <  2:7241472/93‑1 (MQ=255)

                                      cacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAg                                                   >  2:3875905/1‑93 (MQ=255)

                                      cacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAg                                                   >  1:3930008/1‑93 (MQ=255)

                                      cacTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAg                                                   >  1:4540473/1‑93 (MQ=255)

                                       acTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGc                                                  >  1:7998907/1‑93 (MQ=255)

                                       acTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGc                                                  >  2:2360002/1‑93 (MQ=255)

                                         tataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAg                                                >  1:445332/1‑93 (MQ=255)

                                         tataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAg                                                <  2:8343687/93‑1 (MQ=255)

                                         tataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAg                                                >  1:856588/1‑93 (MQ=255)

                                          ataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAgg                                               <  2:2707483/93‑1 (MQ=255)

                                          ataAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAgg                                               >  2:8750455/1‑93 (MQ=255)

                                           taAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAggg                                              >  1:4384253/1‑93 (MQ=255)

                                             aaGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCa                                            >  1:7751492/1‑93 (MQ=255)

                                             aaGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCa                                            >  2:2264036/1‑93 (MQ=255)

                                             aaGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCa                                            >  1:6804098/1‑93 (MQ=255)

                                                   cGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTa                                      <  1:4855021/93‑1 (MQ=255)

                                                     ttACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATc                                    >  2:1371507/1‑93 (MQ=255)

                                                      tACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATcc                                   <  2:4692449/93‑1 (MQ=255)

                                                        cAAGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTg                                 >  2:3059919/1‑93 (MQ=255)

                                                         aaGTCGACTCATTAACGACTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGt                                <  1:8972705/93‑1 (MQ=255)

                                                          aGTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTc                               >  2:9048041/1‑93 (MQ=255)

                                                           gTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCa                              <  2:4437250/93‑1 (MQ=255)

                                                           gTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCa                              >  2:9852008/1‑93 (MQ=255)

                                                           gTCGACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCa                              <  1:7298323/93‑1 (MQ=255)

                                                              gACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGtt                           <  1:1348566/93‑1 (MQ=255)

                                                              gACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGtt                           <  1:6163949/93‑1 (MQ=255)

                                                              gACTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGtt                           >  2:5898086/1‑93 (MQ=255)

                                                               aCTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTc                          >  1:10042135/1‑93 (MQ=255)

                                                                cTCATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCg                         >  1:9665235/1‑93 (MQ=255)

                                                                  cATTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtt                       <  1:3106665/93‑1 (MQ=255)

                                                                   aTTAACGGCTATTCAGGAAAGGCTTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGttt                      <  2:5535152/93‑1 (MQ=255)

                                                                   aTTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGttt                      <  2:2864631/93‑1 (MQ=255)

                                                                   aTTAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGttt                      >  1:1942193/1‑93 (MQ=255)

                                                                    ttAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtttt                     >  1:7001614/1‑93 (MQ=255)

                                                                    ttAACGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGtttt                     <  2:6527869/93‑1 (MQ=255)

                                                                      aaCGGCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTAc                   <  2:5294420/93‑1 (MQ=255)

                                                                         ggCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCa                <  2:664200/93‑1 (MQ=255)

                                                                          gCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGccc               <  1:8373888/93‑3 (MQ=255)

                                                                          gCTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCAc               >  1:6729662/1‑93 (MQ=255)

                                                                           cTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGCCAGTTCGTTTTACGCAct              >  2:8851235/1‑93 (MQ=255)

                                                                           cTATTCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGCGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCAct              >  1:9139306/1‑93 (MQ=255)

                                                                              ttCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtct           >  1:2536312/1‑93 (MQ=255)

                                                                               tCAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCActtctt          >  1:4946825/1‑93 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         <  2:3448526/93‑1 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         <  2:2096993/93‑1 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         <  1:1538877/93‑1 (MQ=255)

                                                                                cAGGAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACttcttc         <  2:5198995/93‑1 (MQ=255)

                                                                                  ggAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCg       <  1:5714809/93‑1 (MQ=255)

                                                                                  ggAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCg       >  1:3659272/1‑93 (MQ=255)

                                                                                  ggAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCg       <  1:6584343/93‑1 (MQ=255)

                                                                                  ggAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCg       >  1:8467181/1‑93 (MQ=255)

                                                                                   gAAAGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCgg      <  2:1706738/93‑1 (MQ=255)

                                                                                    aaaGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCggg     <  1:7154256/93‑1 (MQ=255)

                                                                                     aaGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCgggt    >  2:6847145/1‑92 (MQ=255)

                                                                                     aaGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGc    <  1:1726019/93‑1 (MQ=255)

                                                                                      aGGCGTCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCt   >  1:1926234/1‑93 (MQ=255)

TTGGTGGTAATGAACGATACGTTTCTGTATGCCGTAAACACTATAAAGAGGCGTTACAAGTCGACTCATTAACGGCTATTCAGGAAAGGCATCGCCACGATTAATAAGAATTTCTTTACTGACAGGGTGAGCAGGGCACTTTTATCCTGTCAGTTCGTTTTACGCACTTCTTCCGGGCTA  >  NC_000913/1293264‑1293443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`