BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	8,533	C→T	A99V (GCG→GTG)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	8,533	0	C	T	99.8%	1509.9 / ‑6.0	411	A99V (GCG→GTG)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (207/202); total (207/204)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 421 total aligned reads displayed.

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTA  >  NC_000913/8443‑8622

 gATCGTGGGCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGtgcg                                                                                        <  1:6440899/93‑1 (MQ=255)

  aTCGTGGGCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGt                                                                                       >  2:3732789/1‑93 (MQ=255)

  aTCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGt                                                                                       >  2:4849622/1‑93 (MQ=255)

   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTc                                                                                      >  2:7954220/1‑93 (MQ=255)

     gTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCtt                                                                                    >  1:5147885/1‑93 (MQ=255)

     gTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCtt                                                                                    >  2:1133910/1‑93 (MQ=255)

      tGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCttt                                                                                   >  1:9769195/1‑93 (MQ=255)

       ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTc                                                                                  <  2:6063814/93‑1 (MQ=255)

        gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTcc                                                                                 >  2:5279466/1‑93 (MQ=255)

        gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTcc                                                                                 <  2:6812726/93‑1 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                               <  2:2036335/93‑1 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                               >  2:9195417/1‑93 (MQ=255)

          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTa                                                                               >  1:6800710/1‑93 (MQ=255)

           gcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTAt                                                                              <  2:4525784/93‑1 (MQ=255)

             gaccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGa                                                                            <  1:4132830/93‑1 (MQ=255)

              accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGac                                                                           >  1:10013981/1‑93 (MQ=255)

              accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGac                                                                           >  1:6508075/1‑93 (MQ=255)

                cgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGacac                                                                         <  1:7666406/93‑1 (MQ=255)

                 gacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACAcc                                                                        >  2:5004360/1‑93 (MQ=255)

                 gacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACAcc                                                                        >  1:8337414/1‑93 (MQ=255)

                     aaCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAg                                                                    <  1:8038004/93‑1 (MQ=255)

                      aCTGGTAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGc                                                                   <  1:7215444/93‑1 (MQ=255)

                      aCTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGc                                                                   >  2:8463712/1‑93 (MQ=255)

                       cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGCAGCg                                                                  >  2:214281/1‑93 (MQ=255)

                       cTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCg                                                                  >  1:8077580/1‑93 (MQ=255)

                        tGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGt                                                                 >  2:1280240/1‑93 (MQ=255)

                           cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGTCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCaa                                                              <  2:4717399/93‑1 (MQ=255)

                           cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCaa                                                              <  1:9478922/93‑1 (MQ=255)

                            aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAt                                                             <  2:2882413/93‑1 (MQ=255)

                            aGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAt                                                             <  1:2267816/93‑1 (MQ=255)

                             gTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAAtt                                                            <  1:7765515/93‑1 (MQ=255)

                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                           <  1:5878489/93‑1 (MQ=255)

                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                           >  2:2561176/1‑93 (MQ=255)

                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                           >  2:3245887/1‑93 (MQ=255)

                              tAAATATGGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                                           <  1:5493005/93‑1 (MQ=255)

                                  tatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       >  2:4418329/1‑93 (MQ=255)

                                  tatTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       >  2:3178880/1‑93 (MQ=255)

                                  tatTGGTCTGAAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                       <  2:9600836/93‑1 (MQ=255)

                                   atTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaaa                                                      >  2:9429819/1‑93 (MQ=255)

                                    ttGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAg                                                     <  2:7462168/93‑1 (MQ=255)

                                      ggTCTGGAAATCCTGAAACGGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCa                                                   <  2:7911629/93‑1 (MQ=255)

                                        tCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaa                                                 <  1:1301518/93‑1 (MQ=255)

                                         cTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                                <  1:2652701/93‑1 (MQ=255)

                                         cTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                                <  1:6085210/93‑1 (MQ=255)

                                         cTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                                <  2:1479223/93‑1 (MQ=255)

                                          tGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               >  1:6764574/1‑93 (MQ=255)

                                          cggAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                               <  1:3918373/92‑1 (MQ=255)

                                           ggAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACg                                              >  2:3963817/1‑93 (MQ=255)

                                           ggAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACg                                              >  1:1942534/1‑93 (MQ=255)

                                            gAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGc                                             <  2:6288073/93‑1 (MQ=255)

                                             aaaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGcc                                            <  1:6893450/93‑1 (MQ=255)

                                             aaaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGcc                                            <  2:13571/93‑1 (MQ=255)

                                              aaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                           <  2:7628948/93‑1 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         <  2:6580415/93‑1 (MQ=255)

                                                tCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                         >  2:8806817/1‑93 (MQ=255)

                                                   tGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                      >  1:248233/1‑93 (MQ=255)

                                                   tGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                      <  2:1216525/93‑1 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   >  2:5620451/1‑93 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   <  1:1622848/93‑1 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   >  1:7435578/1‑93 (MQ=255)

                                                      aaCTGGTTCCGGGCCGTATCTCAACTGAAGTAGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                   >  1:214781/1‑93 (MQ=255)

                                                       aCTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCACTTGCGAAAGCAAAACGCCTGATCAAAct                                  >  2:7192868/1‑93 (MQ=255)

                                                        gtGGTACCGGGCCGTATCTCAACTGACGTTGATGTGCGTCTTTCCTAGGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActc                                 <  2:9087199/92‑1 (MQ=255)

                                                        cTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActc                                 >  2:4255995/1‑93 (MQ=255)

                                                         tGGTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActct                                >  2:2867109/1‑93 (MQ=255)

                                                          ggTTCCGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGGAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTa                               <  2:2932847/93‑1 (MQ=255)

                                                              ccGGGCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTAGGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAc                           <  2:865178/93‑1 (MQ=255)

                                                                gggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                         <  1:7359635/93‑1 (MQ=255)

                                                                gggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                         >  1:1214727/1‑93 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                        <  2:4651408/93‑1 (MQ=255)

                                                                 ggCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt                        >  1:9019276/1‑93 (MQ=255)

                                                                  gCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATg                       >  1:9867706/1‑93 (MQ=255)

                                                                  gCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATg                       >  2:8302074/1‑93 (MQ=255)

                                                                  gCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATg                       >  1:5340288/1‑93 (MQ=255)

                                                                  gCCGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATg                       <  1:3849704/93‑1 (MQ=255)

                                                                   ccGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGc                      <  1:4491755/93‑1 (MQ=255)

                                                                    cGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCt                     <  2:5721736/93‑1 (MQ=255)

                                                                    cGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCt                     >  1:4927294/1‑93 (MQ=255)

                                                                    cGTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCt                     >  1:454370/1‑93 (MQ=255)

                                                                     gTATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTg                    >  1:1877817/1‑93 (MQ=255)

                                                                      tATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTgg                   >  1:1145302/1‑93 (MQ=255)

                                                                      tATCTCAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTgg                   >  1:3453109/1‑93 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                 <  1:6160809/93‑1 (MQ=255)

                                                                        tctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCATGATCAAACTCTACAACGATGCTCGTa                 >  1:7070370/1‑93 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                >  2:3450928/1‑93 (MQ=255)

                                                                         ctcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                >  2:9863450/1‑93 (MQ=255)

                                                                          tcAACTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAtt               >  2:8229823/1‑93 (MQ=255)

                                                                            aaCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAg             >  1:8695721/1‑93 (MQ=255)

                                                                            aaCTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAg             <  2:741617/93‑1 (MQ=255)

                                                                              cTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCa           <  1:6655180/93‑1 (MQ=255)

                                                                              cTGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCa           >  2:5918191/1‑93 (MQ=255)

                                                                               tGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCaa          <  2:1751778/93‑1 (MQ=255)

                                                                               tGAAGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCaa          >  2:2254328/1‑93 (MQ=255)

                                                                                 aaGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACg        >  2:7780208/1‑93 (MQ=255)

                                                                                 aaGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACg        <  1:3043560/93‑1 (MQ=255)

                                                                                  aGTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGa       <  1:8173824/93‑1 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt      <  2:1648998/93‑1 (MQ=255)

                                                                                   gTTGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGAt      <  1:3994773/93‑1 (MQ=255)

                                                                                    ttGATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTTCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATc     >  1:3261869/1‑93 (MQ=255)

                                                                                      gATGTGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGt   >  2:8565080/1‑93 (MQ=255)

AGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTA  >  NC_000913/8443‑8622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`