BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,664	A→G	T182T (ACT→ACC)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,664	0	A	G	99.6%	846.7 / ‑4.9	262	T182T (ACT→ACC)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (116/144); total (118/144)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

Only 100 of 267 total aligned reads displayed.

ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG  >  NC_000913/1347574‑1347753

 tGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTa                                                                                        <  1:9763926/93‑1 (MQ=255)

 tGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTa                                                                                        >  2:7296468/1‑93 (MQ=255)

   cccGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGCTCACACAGTCGCAGGCGGTAAg                                                                                      >  1:3996609/1‑93 (MQ=255)

     cGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAc                                                                                    <  2:2775345/93‑1 (MQ=255)

      gagaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAcc                                                                                   >  2:3771175/1‑93 (MQ=255)

       agaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCg                                                                                  >  1:2249656/1‑93 (MQ=255)

       agaTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCg                                                                                  <  1:2723528/93‑1 (MQ=255)

         aTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAc                                                                                >  1:1627902/1‑93 (MQ=255)

         aTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAc                                                                                <  2:8849318/93‑1 (MQ=255)

         aTAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGAc                                                                                >  1:6605233/1‑93 (MQ=255)

          tAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACg                                                                               >  1:6733916/1‑93 (MQ=255)

          tAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACg                                                                               >  2:6698641/1‑93 (MQ=255)

            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             >  1:7801894/1‑93 (MQ=255)

            aGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTc                                                                             <  1:3334420/93‑1 (MQ=255)

             gCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCa                                                                            <  2:9060037/93‑1 (MQ=255)

              ccTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCAt                                                                           >  1:158026/1‑93 (MQ=255)

               cTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTGCCAGGCGGTAAGACCGACGTCAtt                                                                          >  2:982283/1‑93 (MQ=255)

                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         >  2:620286/1‑93 (MQ=255)

                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         <  1:7244828/93‑1 (MQ=255)

                tGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTc                                                                         >  1:7311368/1‑93 (MQ=255)

                 gAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCa                                                                        <  1:8040998/93‑1 (MQ=255)

                   aaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGa                                                                      <  2:1641721/93‑1 (MQ=255)

                   aaTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGa                                                                      >  2:6578972/1‑93 (MQ=255)

                    aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     <  2:26144/93‑1 (MQ=255)

                    aTGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGAt                                                                     >  2:6449886/1‑93 (MQ=255)

                      gTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGc                                                                   <  1:2500776/93‑1 (MQ=255)

                         gCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTgg                                                                >  1:5877878/1‑93 (MQ=255)

                         gCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTgg                                                                <  2:7221903/93‑1 (MQ=255)

                          cAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgc                                                               <  1:9238021/93‑1 (MQ=255)

                            aTTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGgcgc                                                             <  1:6968166/93‑1 (MQ=255)

                             ttGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGcgcg                                                            >  2:7129819/1‑93 (MQ=255)

                               gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGAt                                                          <  2:590819/93‑1 (MQ=255)

                               gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGAt                                                          <  1:7405603/93‑1 (MQ=255)

                               gTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGAt                                                          <  1:1057688/93‑1 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  1:6013658/1‑93 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         <  2:8094588/93‑1 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  2:2017695/1‑93 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  2:7475814/1‑93 (MQ=255)

                                tCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCAAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATc                                                         >  2:3002227/1‑93 (MQ=255)

                                  cgcgCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGTGCGATCgg                                                       <  1:5596675/93‑1 (MQ=255)

                                  cgcgCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCgg                                                       <  1:3965480/93‑1 (MQ=255)

                                   gcgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGt                                                      <  1:9593532/93‑1 (MQ=255)

                                   gcgcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGt                                                      <  1:5751258/93‑1 (MQ=255)

                                     gcCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTg                                                    >  1:9480932/1‑93 (MQ=255)

                                      ccTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGt                                                   <  1:4502404/93‑1 (MQ=255)

                                         gAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTTTCGa                                                >  2:3546648/1‑93 (MQ=255)

                                         gAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGa                                                <  1:1007625/93‑1 (MQ=255)

                                          aaaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGat                                               >  1:9866323/1‑93 (MQ=255)

                                           aaGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGata                                              <  1:3511452/93‑1 (MQ=255)

                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             >  2:434486/1‑93 (MQ=255)

                                            aGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataa                                             <  1:5023370/93‑1 (MQ=255)

                                              ccAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGataata                                           <  1:4810998/93‑1 (MQ=255)

                                               cAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAg                                          <  1:1900075/93‑1 (MQ=255)

                                               cAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAg                                          <  1:9867008/93‑1 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        <  1:1757706/93‑1 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        >  2:1351391/1‑93 (MQ=255)

                                                 aTAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGaa                                        <  2:8419113/93‑1 (MQ=255)

                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       <  2:4398579/93‑1 (MQ=255)

                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       <  1:6569071/93‑1 (MQ=255)

                                                  tAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGAACGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAg                                       >  2:4935629/1‑93 (MQ=255)

                                                     aaCCAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCg                                    <  2:7367604/93‑1 (MQ=255)

                                                       ccAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAg                                  >  1:9190617/1‑93 (MQ=255)

                                                       ccAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAg                                  <  2:2500083/93‑1 (MQ=255)

                                                        cAATGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGc                                 >  1:7972503/1‑93 (MQ=255)

                                                         aaTGATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCt                                >  2:8434688/1‑93 (MQ=255)

                                                            gATTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGc                             >  1:5474530/1‑93 (MQ=255)

                                                             aTTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGcc                            <  2:181823/93‑1 (MQ=255)

                                                             aTTTGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGcc                            <  1:8860431/93‑1 (MQ=255)

                                                              tttGGCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCa                           <  1:6343439/93‑1 (MQ=255)

                                                                 ggCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGtt                        <  2:8865822/93‑1 (MQ=255)

                                                                 ggCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGtt                        <  1:5436472/93‑1 (MQ=255)

                                                                  gCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTg                       >  1:8746903/1‑93 (MQ=255)

                                                                  gCTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTg                       <  2:5133368/93‑1 (MQ=255)

                                                                   cTAATGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTgg                      <  1:1389618/93‑1 (MQ=255)

                                                                     aaTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCg                    >  2:9458472/1‑93 (MQ=255)

                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:3319324/1‑93 (MQ=255)

                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:3612126/1‑93 (MQ=255)

                                                                      aTGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCgg                   >  1:367013/1‑93 (MQ=255)

                                                                       tGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCggg                  >  1:6864472/1‑93 (MQ=255)

                                                                       tGATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCggg                  >  2:2303122/1‑93 (MQ=255)

                                                                        gATCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGc                 >  2:1523794/1‑93 (MQ=255)

                                                                         aTCACACAGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCa                >  2:3035712/1‑93 (MQ=255)

                                                                           cacacaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCata              >  2:4076332/1‑93 (MQ=255)

                                                                             cacaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGTGGGCATAtt            <  2:6737520/93‑1 (MQ=255)

                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           <  1:6174347/93‑1 (MQ=255)

                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           <  1:8295292/93‑1 (MQ=255)

                                                                              acaGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTg           >  2:1726221/1‑93 (MQ=255)

                                                                               caGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGc          >  2:855447/1‑93 (MQ=255)

                                                                                aGTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCg         <  2:7579671/93‑1 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGGCCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  2:3353322/93‑1 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  2:5046639/93‑1 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:1155858/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:9428449/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:4638430/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  1:297609/1‑93 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        <  1:3202615/93‑1 (MQ=255)

                                                                                 gTCCCAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGt        >  2:5004224/1‑93 (MQ=255)

                                                                                    ccAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAt     >  1:7509519/1‑93 (MQ=255)

                                                                                     cAGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAtt    >  2:6018529/1‑93 (MQ=255)

                                                                                      aGGCGGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGAttt   <  1:6898886/93‑1 (MQ=255)

ATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGTCATTCAGATGCTGGCGCGATCGGTTGTCGATAATAGAAGGCGAGCTAGCCAGTTGGCGGGCATATTGCGTGATTTG  >  NC_000913/1347574‑1347753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`