BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,962	C→T	R194H (CGT→CAT)	sapC ←	SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,962	0	C	T	99.6%	847.5 / NA	230	R194H (CGT→CAT)	sapC	SapC
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (113/116); minor base G (0/1); total (113/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

AGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  NC_000913/1351872‑1352051

 gTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATggg                                                                                        >  1:1619524/1‑93 (MQ=255)

  tGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGggc                                                                                       <  1:2262492/93‑1 (MQ=255)

      cgGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGgcggcg                                                                                   <  1:4425906/93‑1 (MQ=255)

       gggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGa                                                                                  >  1:1443782/1‑93 (MQ=255)

       gggTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGa                                                                                  <  1:6361130/93‑1 (MQ=255)

         gTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATa                                                                                <  2:5589106/93‑1 (MQ=255)

         gTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATa                                                                                <  1:9925154/93‑1 (MQ=255)

         gTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATa                                                                                <  1:8827928/93‑1 (MQ=255)

          tGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATaa                                                                               <  1:5610158/93‑1 (MQ=255)

            aTCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACg                                                                             <  2:8222490/93‑1 (MQ=255)

             tctcGGTGACCAGCCCGGCGGTGATGTTTGGCCTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGt                                                                            <  2:7951607/93‑1 (MQ=255)

             tctcGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGt                                                                            >  1:10016027/1‑93 (MQ=255)

             tctcGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGt                                                                            <  1:2935692/93‑1 (MQ=255)

               tcGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTAc                                                                          >  2:4772639/1‑93 (MQ=255)

                cGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTAct                                                                         <  2:5227422/93‑1 (MQ=255)

                 ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActc                                                                        <  2:9692624/93‑1 (MQ=255)

                 ggTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActc                                                                        >  1:3784684/1‑93 (MQ=255)

                  gTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTActct                                                                       <  2:6291266/93‑1 (MQ=255)

                   tGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCtt                                                                      >  2:7534767/1‑93 (MQ=255)

                     aCCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCtttt                                                                    <  2:8785340/93‑1 (MQ=255)

                       cAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTc                                                                  >  1:3841670/1‑93 (MQ=255)

                         gCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                                >  2:5684911/1‑93 (MQ=255)

                         gCCCGGCGGTGATGTATGGCATCCCAGCAAACCTGAGAATATTCAACGTTGATGCGCCATACAGATGGGCGGCGATAACGTACTCTTTTTCCa                                                                <  1:8979566/93‑1 (MQ=255)

                            cggcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGtt                                                             >  1:8520052/1‑93 (MQ=255)

                             ggcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTc                                                            <  1:9810447/93‑1 (MQ=255)

                              gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGTCGGCGATAACGTACTTTTTTTCCAGTTCg                                                           >  1:8412254/1‑93 (MQ=255)

                              gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCg                                                           >  1:9445651/1‑93 (MQ=255)

                              gcggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCg                                                           <  2:4900572/93‑1 (MQ=255)

                               cggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCAAGTTCGt                                                          >  2:1914995/1‑93 (MQ=255)

                                ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTTGTc                                                         >  2:5692712/1‑93 (MQ=255)

                                ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTc                                                         >  1:2491621/1‑93 (MQ=255)

                                ggTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTc                                                         <  2:9082538/93‑1 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        >  1:2661526/1‑93 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        <  2:5853060/93‑1 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        >  2:8996545/1‑93 (MQ=255)

                                 gTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCa                                                        <  2:964901/93‑1 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       >  2:2864614/1‑93 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       >  2:8202229/1‑93 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       <  1:8224936/93‑1 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       <  1:7108942/93‑1 (MQ=255)

                                  tGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCAt                                                       <  1:5430579/93‑1 (MQ=255)

                                    aTGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGc                                                     >  1:4071086/1‑93 (MQ=255)

                                     tGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCa                                                    >  1:4851265/1‑93 (MQ=255)

                                     tGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCa                                                    >  2:3098494/1‑93 (MQ=255)

                                     tGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCa                                                    >  2:2219753/1‑93 (MQ=255)

                                      gTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAc                                                   >  1:6400388/1‑93 (MQ=255)

                                      gTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAc                                                   <  2:8008001/93‑1 (MQ=255)

                                       tttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAcc                                                  >  1:2810476/1‑93 (MQ=255)

                                       tttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAcc                                                  >  1:5099204/1‑93 (MQ=255)

                                       tttGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCAcc                                                  >  1:7234920/1‑93 (MQ=255)

                                         tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAt                                                >  1:9174929/1‑93 (MQ=255)

                                         tGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCAt                                                <  1:1089383/93‑1 (MQ=255)

                                          tgCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATg                                               <  1:1998043/92‑1 (MQ=255)

                                           gCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGTCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGc                                              >  2:4168026/1‑93 (MQ=255)

                                            cATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCt                                             <  2:3657205/93‑1 (MQ=255)

                                            cATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCt                                             >  2:3821065/1‑93 (MQ=255)

                                             aTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTg                                            >  1:7893799/1‑93 (MQ=255)

                                             aTCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTg                                            <  1:1266803/93‑1 (MQ=255)

                                              tCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGt                                           >  2:4053701/1‑93 (MQ=255)

                                                  aGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAAt                                       <  2:6665821/93‑1 (MQ=255)

                                                   gCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATc                                      <  2:2923082/93‑1 (MQ=255)

                                                    cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                     >  2:328229/1‑93 (MQ=255)

                                                    cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                     <  2:6745638/93‑1 (MQ=255)

                                                    cAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCg                                     <  2:7624036/93‑1 (MQ=255)

                                                     aaaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGa                                    >  1:9064065/1‑93 (MQ=255)

                                                      aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                   >  2:5564/1‑93 (MQ=255)

                                                      aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                   <  1:1037377/93‑1 (MQ=255)

                                                      aaCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGaa                                   >  2:2494466/1‑93 (MQ=255)

                                                       aCCAGAGAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAAc                                  >  1:6292799/1‑93 (MQ=255)

                                                          agagAATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTa                               <  2:2770634/93‑1 (MQ=255)

                                                           gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                              <  2:7895397/93‑1 (MQ=255)

                                                           gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                              >  2:1030051/1‑93 (MQ=255)

                                                           gagaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTAc                              <  1:670915/93‑1 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            >  1:1407048/1‑93 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            <  2:5452048/93‑1 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            >  2:7432048/1‑93 (MQ=255)

                                                             gaATATTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCa                            <  1:2176189/93‑1 (MQ=255)

                                                               atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                          <  1:3486019/93‑1 (MQ=255)

                                                               atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                          <  2:8782758/93‑1 (MQ=255)

                                                               atatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATa                          <  2:7229359/93‑1 (MQ=255)

                                                                tatTCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAc                         <  1:7647458/93‑1 (MQ=255)

                                                                   tCAGCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATAcgcg                      >  2:8577543/1‑93 (MQ=255)

                                                                      gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgca                   <  1:5016001/93‑1 (MQ=255)

                                                                      gCGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgca                   >  2:3327235/1‑93 (MQ=255)

                                                                       cGTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGCTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgcag                  >  1:7599971/1‑93 (MQ=255)

                                                                        gTTGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGgcagc                 <  1:841230/93‑1 (MQ=255)

                                                                          ttaTGCCCCAACCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCaa               <  2:8654738/91‑1 (MQ=255)

                                                                          tGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCaa               <  1:8176428/93‑1 (MQ=255)

                                                                          tGATGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCaa               <  1:597009/93‑1 (MQ=255)

                                                                             tGCGCCATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgc            >  2:1438461/1‑93 (MQ=255)

                                                                                  cATCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcc       >  1:4650901/1‑93 (MQ=255)

                                                                                   aTCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcca      >  2:2367062/1‑93 (MQ=255)

                                                                                   aTCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATgccagcca      >  1:1237843/1‑93 (MQ=255)

                                                                                    tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag     >  2:6519107/1‑93 (MQ=255)

                                                                                    tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag     >  1:5825126/1‑93 (MQ=255)

                                                                                    tCCAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGccagccag     >  2:395881/1‑93 (MQ=255)

                                                                                      cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc   >  1:1175842/1‑93 (MQ=255)

                                                                                      cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc   <  1:5185149/93‑1 (MQ=255)

                                                                                      cAGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGAc   <  2:4981857/93‑1 (MQ=255)

                                                                                       aGATGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACa  >  2:4061155/1‑93 (MQ=255)

AGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATGCGCCATCCAGACGGGCGGCGATAACGTACTCTTTTTCCAGTTCGTCATGCACCATGCTGTAAATCGAACGTACCATACGCGGCAGCAATGCCAGCCAGACA  >  NC_000913/1351872‑1352051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`