BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,126	T→C	M252V (ATG→GTG)	b1341 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,126	0	T	C	99.7%	1002.3 / ‑5.3	310	M252V (ATG→GTG)	b1341	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (164/145); total (164/146)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 322 total aligned reads displayed.

GTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACATGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG  >  NC_000913/1405036‑1405216

gTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGa                                                                                          <  2:7877286/93‑1 (MQ=255)

gTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGa                                                                                          >  1:5644442/1‑93 (MQ=255)

 tAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAg                                                                                         >  2:100715/1‑93 (MQ=255)

  aaTCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGc                                                                                        >  1:7024504/1‑93 (MQ=255)

   aTCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCt                                                                                       <  1:3637790/93‑1 (MQ=255)

   aTCCGGTCATCGCGTAATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCt                                                                                       <  2:4260097/93‑1 (MQ=255)

    tCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCtt                                                                                      >  1:1226079/1‑93 (MQ=255)

     ccGGTCATCGCGTCATGGTGAGCAGCTTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTg                                                                                     <  2:9333601/93‑1 (MQ=255)

     ccGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTg                                                                                     >  2:2513126/1‑93 (MQ=255)

         tCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGc                                                                                 >  1:7459601/1‑93 (MQ=255)

         tCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGc                                                                                 >  2:6416845/1‑93 (MQ=255)

           aTCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCa                                                                               >  2:5078087/1‑93 (MQ=255)

            tCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCAt                                                                              >  2:3874423/1‑93 (MQ=255)

            tCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCAt                                                                              >  1:353823/1‑93 (MQ=255)

             cgcgTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATa                                                                             <  2:2556989/93‑1 (MQ=255)

              gcgTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATaa                                                                            >  1:9968309/1‑93 (MQ=255)

               cgTCATGGTGAGCAGCATGTTCCTGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATaaa                                                                           <  2:7231859/93‑1 (MQ=255)

                   aTGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTc                                                                       >  1:4413211/1‑93 (MQ=255)

                     ggTTAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCaa                                                                     <  2:3979563/93‑1 (MQ=255)

                     ggTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCaa                                                                     <  2:1837436/93‑1 (MQ=255)

                       tGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATc                                                                   >  2:9638511/1‑93 (MQ=255)

                          gcagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGt                                                                >  2:862096/1‑93 (MQ=255)

                          gcagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGt                                                                >  2:9217152/1‑93 (MQ=255)

                          gcagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGt                                                                >  1:2634546/1‑93 (MQ=255)

                           cagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTc                                                               <  2:5993346/93‑1 (MQ=255)

                           cagcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTc                                                               >  2:129001/1‑93 (MQ=255)

                           cagcaTGTTCCAGCTGCTCCTCCAGCCGTTGTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTGACTCGGTc                                                               >  1:2952093/1‑93 (MQ=255)

                             gcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATACATTTCAATCGGTCCg                                                             >  1:2199284/1‑93 (MQ=255)

                             gcaTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCg                                                             >  1:6691997/1‑93 (MQ=255)

                             gcaTGTTACAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCg                                                             <  1:9296091/93‑1 (MQ=255)

                              caTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCgg                                                            <  2:55696/93‑1 (MQ=255)

                               aTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGc                                                           >  1:6019389/1‑93 (MQ=255)

                               aTGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGc                                                           <  2:3941292/93‑1 (MQ=255)

                                tGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          >  2:6717024/1‑93 (MQ=255)

                                tGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          <  2:569297/93‑1 (MQ=255)

                                tGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          >  1:7631075/1‑93 (MQ=255)

                                tGTTCCAGCTGCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCa                                                          <  1:4960078/93‑1 (MQ=255)

                                  ttCCAGCTCCCCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATa                                                        <  1:3758922/93‑1 (MQ=255)

                                    ccAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAgg                                                      >  1:7706273/1‑93 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    <  1:7969664/93‑1 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    <  2:7400622/93‑1 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    <  1:9490080/93‑1 (MQ=255)

                                      aGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTc                                                    >  2:6527282/1‑93 (MQ=255)

                                         tgctCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGc                                                 <  2:5835953/93‑1 (MQ=255)

                                         tgctCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGc                                                 <  2:6135418/93‑1 (MQ=255)

                                         tgctCCCCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGc                                                 <  2:4106644/93‑1 (MQ=255)

                                          gctCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGca                                                >  2:1503361/1‑93 (MQ=255)

                                            tcctccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGcaca                                              <  2:8226211/93‑1 (MQ=255)

                                            ccctccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGcaca                                              <  1:3380384/92‑1 (MQ=255)

                                             cctccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACAt                                             >  1:2011377/1‑93 (MQ=255)

                                             catccAGCCTTTTTTGCTCAGTAATATCAGGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACAt                                             <  2:4408778/91‑1 (MQ=255)

                                               tccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGa                                           >  2:287896/1‑93 (MQ=255)

                                               tccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGa                                           >  1:2357935/1‑93 (MQ=255)

                                                ccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAc                                          >  2:7806126/1‑93 (MQ=255)

                                                ccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAc                                          >  1:6842207/1‑93 (MQ=255)

                                                ccAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGAc                                          >  2:2768285/1‑93 (MQ=255)

                                                  aGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGa                                        >  1:8684959/1‑93 (MQ=255)

                                                  aGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGa                                        >  1:6522220/1‑93 (MQ=255)

                                                   gCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAg                                       <  2:2158377/93‑1 (MQ=255)

                                                   gCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAg                                       >  1:4564354/1‑93 (MQ=255)

                                                   gCCGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAg                                       <  2:1354923/93‑1 (MQ=255)

                                                     cGTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTc                                     <  2:4186865/93‑1 (MQ=255)

                                                      gTTTTTGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCg                                    >  2:9848230/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  1:5199345/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  1:2511042/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  2:6168843/1‑93 (MQ=255)

                                                       tttttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGa                                   >  1:9713594/1‑93 (MQ=255)

                                                         tttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAc                                 <  1:4830352/93‑1 (MQ=255)

                                                         tttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAc                                 <  1:6371571/93‑1 (MQ=255)

                                                          ttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAcc                                >  2:5301185/1‑93 (MQ=255)

                                                          ttGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAAcc                                <  2:3067685/93‑1 (MQ=255)

                                                           tGCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCa                               <  2:4706254/93‑1 (MQ=255)

                                                            gCTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAt                              <  1:3181402/93‑1 (MQ=255)

                                                             cTCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATc                             >  2:3286296/1‑93 (MQ=255)

                                                              tCAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATcc                            >  2:8336785/1‑93 (MQ=255)

                                                               cAGTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCg                           >  1:1577695/1‑93 (MQ=255)

                                                                 gTAATATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGcc                         >  2:9132436/1‑93 (MQ=255)

                                                                   aaTATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAg                       >  1:8461853/1‑93 (MQ=255)

                                                                   aaTATCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAg                       >  1:307967/1‑93 (MQ=255)

                                                                    atatCAAGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGt                      >  2:3116039/1‑93 (MQ=255)

                                                                     tatCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGtt                     <  2:3799342/93‑1 (MQ=255)

                                                                     tatCATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGtt                     >  2:4886549/1‑93 (MQ=255)

                                                                        cATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTAt                  <  1:2806376/93‑1 (MQ=255)

                                                                        cATGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTAt                  >  1:1278690/1‑93 (MQ=255)

                                                                         aTGCACAATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATg                 <  1:1007499/93‑1 (MQ=255)

                                                                             acaATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACa             <  1:7839234/93‑1 (MQ=255)

                                                                             acaATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACa             >  2:899285/1‑93 (MQ=255)

                                                                              caATACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACaa            >  1:6599213/1‑93 (MQ=255)

                                                                               aaTACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACaaa           <  1:573769/93‑1 (MQ=255)

                                                                                aTACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACaaaa          >  2:9240149/1‑93 (MQ=255)

                                                                                aTACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCAGCCGCCAGTTTATGAACaaaa          <  2:9683861/93‑1 (MQ=255)

                                                                                 tACATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAAt         >  2:7711089/1‑93 (MQ=255)

                                                                                   cATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAACATTc       >  2:8125229/1‑93 (MQ=255)

                                                                                   cATAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTc       >  1:1423256/1‑93 (MQ=255)

                                                                                    aTAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCa      >  2:6987416/1‑93 (MQ=255)

                                                                                    aTAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCa      <  1:3975154/93‑1 (MQ=255)

                                                                                     tAACACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCaa     >  1:8094385/1‑93 (MQ=255)

                                                                                      aaCACGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCaaa    <  2:9775617/93‑1 (MQ=255)

                                                                                       acacGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAg   <  1:5480593/93‑1 (MQ=255)

                                                                                       acacGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAg   >  1:9028438/1‑93 (MQ=255)

GTAATCCGGTCATCGCGTCATGGTGAGCAGCATGTTCCAGCTGCTCCTCCAGCCGTTTTTGCTCAGTAATATCATGCACAATACATAACATGAGCTTGTCGCCATAAATTTCAATCGGTCCGGCATAGGTCTGCACATGACGAGTCGAACCATCCGCCAGTTTATGAACAAAATTCAAAGG  >  NC_000913/1405036‑1405216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`