BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,426,410	T→C	Q114R (CAG→CGG)	trs55 ←	IS5 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,426,410	0	T	C	93.5%	510.3 / 20.8	185	Q114R (CAG→CGG)	trs55	IS5 transposase
Reads supporting (aligned to +/- strand): ref base T (6/6); new base C (98/74); total (104/81)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

Only 100 of 195 total aligned reads displayed.

GGCATCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCTGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAGGCG  >  NC_000913/1426319‑1426499

ggCATCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGAtgctg                                                                                          <  1:6377321/93‑1 (MQ=255)

 gCATCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGAtgccgc                                                                                         <  1:1984166/93‑1 (MQ=255)

   aTCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTc                                                                                       >  1:550688/1‑93 (MQ=255)

   aTCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTc                                                                                       <  1:95987/93‑1 (MQ=255)

   aTCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTc                                                                                       <  1:3589154/93‑1 (MQ=255)

    tCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCg                                                                                      >  2:6648618/1‑93 (MQ=255)

    tCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCg                                                                                      >  1:3842360/1‑93 (MQ=255)

      caccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTcagg                                                                                    >  1:1144706/1‑90 (MQ=255)

      caccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAg                                                                                    >  2:5447097/1‑93 (MQ=255)

       cccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCTGCTCGAGc                                                                                   <  2:7104473/92‑1 (MQ=255)

       accaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGc                                                                                   <  2:6337123/93‑1 (MQ=255)

       accaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGc                                                                                   >  1:4741319/1‑93 (MQ=255)

        ccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCa                                                                                  >  1:388930/1‑93 (MQ=255)

        ccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCa                                                                                  <  2:4319339/93‑1 (MQ=255)

        ccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCa                                                                                  <  2:2862961/93‑1 (MQ=255)

        ccaAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCa                                                                                  <  2:3008700/93‑1 (MQ=255)

         caAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAg                                                                                 <  1:5268842/93‑1 (MQ=255)

          aaaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAgg                                                                                <  1:4105834/93‑1 (MQ=255)

          aaaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAgg                                                                                >  1:6637369/1‑93 (MQ=255)

          aaaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAgg                                                                                <  1:4758665/93‑1 (MQ=255)

          aaaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAgg                                                                                >  2:321603/1‑93 (MQ=255)

           aaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGt                                                                               <  1:3686797/93‑1 (MQ=255)

           aaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGt                                                                               <  1:8555562/93‑1 (MQ=255)

           aaGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGt                                                                               <  1:6489577/93‑1 (MQ=255)

            aGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTg                                                                              >  1:4377887/1‑93 (MQ=255)

             gTGGCTTGGGTGATTATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTgg                                                                             <  2:7933400/93‑1 (MQ=14)

             gTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTgg                                                                             >  1:7680728/1‑93 (MQ=255)

             gTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTgg                                                                             <  2:1065485/93‑1 (MQ=255)

             gTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTgg                                                                             <  1:1289613/93‑1 (MQ=255)

              tGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGc                                                                            >  2:228956/1‑93 (MQ=255)

                ccTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCgg                                                                          <  2:86954/93‑1 (MQ=255)

                  ttGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGaa                                                                        >  2:6904735/1‑93 (MQ=255)

                       tcatcaTGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCa                                                                   >  2:4228431/1‑93 (MQ=37)

                       tcatcaTGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCa                                                                   <  1:941316/93‑1 (MQ=255)

                       gcatcatGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCa                                                                   <  1:8692086/92‑1 (MQ=255)

                        catcatGACGCCTGCTTTGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCat                                                                  >  1:969920/1‑93 (MQ=255)

                        catcatGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCTGCTCGAGCAGGTGGCGGAAATTCat                                                                  <  2:7662045/93‑1 (MQ=38)

                          tcatGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGGGGCGGAAATTCatga                                                                >  2:3167383/1‑93 (MQ=255)

                            atGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCatgatg                                                              <  1:8787065/93‑1 (MQ=255)

                             tGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGAtgg                                                             <  2:554130/93‑1 (MQ=37)

                              gACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGAtggt                                                            <  1:6429902/93‑1 (MQ=255)

                              gACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGAtggg                                                            >  2:1997789/1‑92 (MQ=255)

                               aCGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGAtggtg                                                           <  2:608538/93‑1 (MQ=255)

                               aCGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCAAGTTGATGCAGCTCGAGCAGGTGGCGGAAATTCATGAtggtg                                                           <  2:2081262/93‑1 (MQ=255)

                                 gCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATggtggt                                                         <  1:3432853/93‑1 (MQ=255)

                                  ccTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCTGCTCGAGCAGGTGGCGGAAATTCATGATGgtggtg                                                        <  2:3845738/93‑1 (MQ=38)

                                  ccTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGgtggtg                                                        <  1:2543178/93‑1 (MQ=255)

                                      cTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGAt                                                    <  2:9430289/93‑1 (MQ=255)

                                       ttCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCTGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATc                                                   >  2:2529797/1‑93 (MQ=255)

                                       ttCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATc                                                   >  2:296823/1‑93 (MQ=255)

                                          ggCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCgg                                                <  1:9025359/93‑1 (MQ=255)

                                                ccagcGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCTCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGgc                                          >  1:4589123/1‑93 (MQ=255)

                                                ccagcGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGAGACCCGGCAGGgc                                          >  2:3414337/1‑93 (MQ=255)

                                                 cagcGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGgcg                                         >  1:3075497/1‑93 (MQ=255)

                                                 cagcGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGgcg                                         >  1:716204/1‑93 (MQ=255)

                                                    cGATTGATGGTCTTGAACCATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCtt                                      >  2:5702444/1‑92 (MQ=255)

                                                     gATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTAt                                     >  2:1668642/1‑93 (MQ=255)

                                                     gATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTAt                                     >  2:2731310/1‑93 (MQ=255)

                                                     gATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTAt                                     >  2:9577134/1‑93 (MQ=255)

                                                       ttGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGACGGAAATTCATGATGGTGGTGCGATCCGGACGGGCGCTATcc                                   >  2:2451344/1‑93 (MQ=14)

                                                        tGATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCa                                  >  1:10013049/1‑93 (MQ=255)

                                                         gATGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAg                                 >  2:4033106/1‑93 (MQ=255)

                                                          aTGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAgg                                >  1:7975583/1‑93 (MQ=255)

                                                          aTGGTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAgg                                >  2:8012521/1‑93 (MQ=255)

                                                            ggTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGa                              <  2:187399/93‑1 (MQ=255)

                                                            ggTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGa                              >  2:4484021/1‑93 (MQ=255)

                                                            ggTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGa                              <  1:857947/93‑1 (MQ=255)

                                                             gTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGAt                             <  2:53706/93‑1 (MQ=255)

                                                             gTCTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGAt                             >  2:9867545/1‑93 (MQ=255)

                                                               cTTGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATaa                           <  2:1333010/93‑1 (MQ=255)

                                                                ttGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAAt                          <  1:3985594/93‑1 (MQ=255)

                                                                ttGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAAt                          <  1:1887409/93‑1 (MQ=255)

                                                                 tGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATc                         <  1:10022105/93‑1 (MQ=255)

                                                                 tGAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATc                         <  2:4725835/93‑1 (MQ=255)

                                                                  gAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCg                        >  1:8640615/1‑93 (MQ=255)

                                                                  gAACAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCg                        >  1:8632368/1‑93 (MQ=255)

                                                                     cAATTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGc                     >  1:3845339/1‑93 (MQ=255)

                                                                       aTTGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCaa                   >  1:7879411/1‑93 (MQ=255)

                                                                        ttGACGGGCCAGTTGATGCCTCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCaaa                  <  1:2759595/93‑1 (MQ=255)

                                                                         tGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAAc                 >  2:7353504/1‑93 (MQ=255)

                                                                         tGACGGGCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAAc                 >  1:6118284/1‑93 (MQ=255)

                                                                             gggCCAGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGgc             >  1:9281075/1‑93 (MQ=255)

                                                                                  aGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATg        >  2:1408683/1‑93 (MQ=255)

                                                                                  aGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATg        <  2:2388413/93‑1 (MQ=255)

                                                                                  aGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATg        <  1:3306395/93‑1 (MQ=255)

                                                                                  aGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATg        >  1:7783830/1‑93 (MQ=255)

                                                                                  aGTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATg        >  1:5018127/1‑93 (MQ=255)

                                                                                   gTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATgg       <  1:74508/93‑1 (MQ=255)

                                                                                   gTTGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATgg       >  2:1220550/1‑93 (MQ=255)

                                                                                   gTTGATGCCGCTCGAGCAGGAGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATgg       >  2:8175334/1‑93 (MQ=255)

                                                                                    ttGATGCTGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGa      >  2:8375625/1‑93 (MQ=255)

                                                                                    ttGATGCTGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGa      >  2:9013242/1‑93 (MQ=255)

                                                                                    ttGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCa                                  >  2:6489517/1‑65 (MQ=255)

                                                                                    ttGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGa      >  1:5900421/1‑93 (MQ=255)

                                                                                     tGATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCGGGGATAATCGGGCAAACAGGCGCATGGAg     <  2:3809634/93‑1 (MQ=255)

                                                                                      gATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAgg    >  2:3365808/1‑93 (MQ=255)

                                                                                      gATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAgg    <  1:8255102/93‑1 (MQ=255)

                                                                                      gATGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAgg    <  2:2509216/93‑1 (MQ=255)

                                                                                       aTGCCGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAGGc   >  1:479679/1‑93 (MQ=255)

                                                                                        tgccgcTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAGGCg  >  2:3577298/1‑93 (MQ=255)

GGCATCCACCAAAGTGCCTTGGGTCATCATGACGCCTGCTTCGGCCAGCCAGCGATTGATGGTCTTGAACAATTGACGGGCCAGTTGATGCTGCTCGAGCAGGTGGCGGAAATTCATGATGGTGGTGCGATCCGGCAGGGCGCTATCCAGGGATAATCGGGCAAACAGGCGCATGGAGGCG  >  NC_000913/1426319‑1426499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`