Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,427,447 A→G A127A (GCA→GCG)  b1372 → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,427,4470AG87.4% 381.1 / 43.5 166A127A (GCA→GCG) b1372putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (7/14);  new base G (70/75);  total (77/89)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

Only 100 of 178 total aligned reads displayed.

GGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCACGCGCAGCCAG  >  NC_000913/1427357‑1427539
                                                                                          |                                                                                            
ggaTGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACggcggc                                                                                            >  2:6971328/1‑93 (MQ=255)
ggaTGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACggcggc                                                                                            <  2:6896160/93‑1 (MQ=255)
 gatgatGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGcc                                                                                           <  1:6193203/93‑1 (MQ=255)
 gatgatGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGcc                                                                                           <  1:3238098/93‑1 (MQ=255)
   tgatgCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCcgc                                                                                         <  2:1773326/93‑1 (MQ=255)
    gatgCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCcgcg                                                                                        >  1:3075017/1‑93 (MQ=255)
        cccGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCgaaga                                                                                    <  1:4064323/93‑1 (MQ=255)
         ccGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCgaagaa                                                                                   <  1:3712136/93‑1 (MQ=255)
          cGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGaagaag                                                                                  <  1:6351773/93‑1 (MQ=255)
          cGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGaagaag                                                                                  >  2:5427438/1‑93 (MQ=255)
            tCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTc                                                                                >  2:4065559/1‑93 (MQ=255)
            tCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTc                                                                                <  1:10027789/93‑1 (MQ=255)
            tCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTc                                                                                >  1:7016908/1‑93 (MQ=255)
             ccGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCTCAGAACACGGCAGTCGCGAAGAAGTCa                                                                               <  2:1614018/93‑1 (MQ=255)
              cGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAg                                                                              >  2:504865/1‑93 (MQ=255)
              cGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAg                                                                              >  2:8631322/1‑93 (MQ=255)
               ggAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGc                                                                             >  1:2825323/1‑93 (MQ=255)
                  ggCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGGGGCGCGTAACGCGTCCGCGGGGTCACAGTAAACGGCGGCCGCGAAGAAGCCAGCAAg                                                                          >  2:718954/1‑93 (MQ=255)
                    cACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTg                                                                        <  2:6180217/93‑1 (MQ=255)
                       tGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGTTGGGACAGAACACGGGGGCGCCGAAGAAGTCAGCCGGtattc                                                                     >  1:6039485/1‑89 (MQ=255)
                        gcgcCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGc                                                                    <  2:6161746/93‑1 (MQ=255)
                        gcgcCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGc                                                                    <  1:7295302/93‑1 (MQ=255)
                        gcgcCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGc                                                                    >  1:1259337/1‑93 (MQ=255)
                        gcgcCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGCGATGc                                                                    >  2:6601222/1‑93 (MQ=255)
                          gcCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCa                                                                  >  2:3191128/1‑93 (MQ=255)
                          gcCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCa                                                                  <  1:2570201/93‑1 (MQ=255)
                          cccGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCa                                                                  <  1:8286488/92‑1 (MQ=255)
                            cGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGc                                                                >  1:7306870/1‑93 (MQ=255)
                              ttttGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGcac                                                              >  2:109191/1‑93 (MQ=255)
                              ttttGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGcac                                                              >  2:7987495/1‑93 (MQ=255)
                               tttGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGcaca                                                             <  1:9759200/93‑1 (MQ=255)
                               tttGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGcaca                                                             <  2:9880847/93‑1 (MQ=255)
                                ttGAAGCGATGGTGGAAGAGGTGGGGCGTAACGCGTCCGCGGTGGCCCAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACAt                                                            <  2:8665485/93‑1 (MQ=255)
                                ttGAACTGATGGCGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAATTCAGCCAGTGATGCCAGcacac                                                            >  2:5616395/1‑92 (MQ=255)
                                 tGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATc                                                           <  2:3861174/93‑1 (MQ=255)
                                     cTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTAAGCCAGTGATGCCAGCACATCAGcc                                                       >  1:9102302/1‑93 (MQ=255)
                                      tGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGccc                                                      <  2:2850890/93‑1 (MQ=255)
                                      tGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGccc                                                      >  1:7228480/1‑93 (MQ=255)
                                      tGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGccc                                                      >  1:2670610/1‑93 (MQ=255)
                                       gATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCg                                                     <  1:1662943/93‑1 (MQ=255)
                                        aTGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGACAGTGATGCCAGCACATCAGCCCGt                                                    <  1:2842632/93‑1 (MQ=255)
                                         tggtggAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTg                                                   <  2:4985690/93‑1 (MQ=255)
                                          ggtggAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGa                                                  <  1:1955650/93‑1 (MQ=255)
                                          ggtggAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGa                                                  <  2:4829357/93‑1 (MQ=255)
                                           gtggAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAg                                                 <  2:5070244/93‑1 (MQ=255)
                                           gtggAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAg                                                 <  1:565203/93‑1 (MQ=255)
                                             ggAAGAGGTGGCGCGTAACGCGTCCGCGCCCGCCCACCACACCGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggc                                               >  2:6968924/1‑93 (MQ=255)
                                              gAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggcg                                              >  1:6792477/1‑93 (MQ=255)
                                              gAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggcg                                              >  2:2078077/1‑93 (MQ=255)
                                              gAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggcg                                              >  1:8935091/1‑93 (MQ=255)
                                              gAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggcg                                              <  2:5740800/93‑1 (MQ=255)
                                               aaGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAggcgg                                             <  1:5722474/93‑1 (MQ=255)
                                                 gagGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCa                                           >  1:5982196/1‑93 (MQ=255)
                                                  agGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGGGGCac                                          >  1:1911355/1‑92 (MQ=255)
                                                     tGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAAccc                                       >  2:1529142/1‑93 (MQ=255)
                                                      ggCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCa                                      <  2:1423531/93‑1 (MQ=255)
                                                       gcgcGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCCACCCAt                                     >  1:6736936/1‑93 (MQ=255)
                                                       gcgcGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCAt                                     <  1:9291301/93‑1 (MQ=255)
                                                       gcgcGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCAt                                     <  1:7899216/93‑1 (MQ=255)
                                                       gcgcGTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCAt                                     <  2:8434296/93‑1 (MQ=255)
                                                        cgcgTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATg                                    <  1:9538506/93‑1 (MQ=255)
                                                         gcgTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGc                                   <  2:9110870/93‑1 (MQ=255)
                                                         gcgTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGc                                   >  1:5422444/1‑93 (MQ=255)
                                                          cgTAACGCGTCCGCGGTGGCACAGAACCCGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCg                                  <  2:7067326/93‑1 (MQ=255)
                                                           gTAACGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCgg                                 <  2:4378373/93‑1 (MQ=255)
                                                           gTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCCTGCgg                                 >  1:6073589/1‑93 (MQ=255)
                                                             aaCGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCt                               <  1:24263/93‑1 (MQ=255)
                                                              aCGCGTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTg                              >  1:10028966/1‑93 (MQ=255)
                                                              aCGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTg                              >  1:3420300/1‑93 (MQ=255)
                                                               cgcgTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGa                             <  1:432801/93‑1 (MQ=255)
                                                               cgcgTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGa                             <  1:871018/93‑1 (MQ=255)
                                                               cgcgTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGa                             <  1:5687606/93‑1 (MQ=255)
                                                                gcgTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAt                            <  1:4429104/93‑1 (MQ=255)
                                                                 cgTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAtg                           <  1:7755107/93‑1 (MQ=255)
                                                                  gTCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAtgc                          <  1:1783157/93‑1 (MQ=255)
                                                                   tCCGCGGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAtgct                         >  1:2349720/1‑93 (MQ=255)
                                                                    ccGCGGTGGCACAGAACTCGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAtgctg                        <  1:8330970/93‑1 (MQ=255)
                                                                     cgcgGTGGCACAGAACACGGCTGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAtgctgc                       <  2:9618459/93‑1 (MQ=255)
                                                                     cgcgGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGAtgctgc                       <  2:8231994/93‑1 (MQ=255)
                                                                      gcgGTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCg                      >  2:2734978/1‑93 (MQ=255)
                                                                        ggTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGa                    >  2:7241981/1‑93 (MQ=255)
                                                                         gTGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGAc                   >  2:1150888/1‑93 (MQ=255)
                                                                          tGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACt                  >  2:716427/1‑93 (MQ=255)
                                                                          tGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACt                  >  1:5777181/1‑93 (MQ=255)
                                                                          tGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACt                  <  2:6808195/93‑1 (MQ=255)
                                                                          tGGCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACt                  <  2:6808193/93‑1 (MQ=255)
                                                                           ggCACAGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTc                 <  1:3169107/93‑1 (MQ=255)
                                                                             cacaGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAg               >  2:7399999/1‑93 (MQ=255)
                                                                             cacaGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAg               <  2:7757924/93‑1 (MQ=255)
                                                                               caGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCa             >  2:2912127/1‑93 (MQ=255)
                                                                                aGAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAc            >  1:2290065/1‑93 (MQ=255)
                                                                                 gAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAcg           >  2:4618351/1‑93 (MQ=255)
                                                                                 gAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAcg           <  1:4796784/93‑1 (MQ=255)
                                                                                 gAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAcg           >  1:1011708/1‑93 (MQ=255)
                                                                                 gAACACGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAcg           >  2:162432/1‑93 (MQ=255)
                                                                                  aaCACGGCGCCCGCGAAGAAGCCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAcgc          <  1:2949191/93‑1 (MQ=255)
                                                                                   acacGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCAcgcg         >  1:8451435/1‑93 (MQ=255)
                                                                                    cacGGCGGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCACgcgc        <  2:9251176/93‑1 (MQ=255)
                                                                                         cAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCACGCGcagcca   <  1:7365677/93‑1 (MQ=255)
                                                                                          aGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCACGCGcagccag  >  1:5244614/1‑93 (MQ=255)
                                                                                          |                                                                                            
GGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGCGGCTGATGCTGCGGACTCAGCACGCGCAGCCAG  >  NC_000913/1427357‑1427539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑