BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,448,839	G→A	P179S (CCG→TCG)	tynA ←	copper amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,448,839	0	G	A	91.2%	961.9 / 42.7	321	P179S (CCG→TCG)	tynA	copper amine oxidase
Reads supporting (aligned to +/- strand): ref base G (20/8); new base A (137/155); total (158/163)
Fisher's exact test for biased strand distribution p-value = 1.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 328 total aligned reads displayed.

GTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGT  >  NC_000913/1448749‑1448930

 ttGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTg                                                                                          <  1:8742287/93‑1 (MQ=255)

  tGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTgg                                                                                         <  1:2440531/93‑1 (MQ=255)

   gCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGt                                                                                        <  2:4387387/93‑1 (MQ=255)

    ccAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTc                                                                                       <  2:9691767/93‑1 (MQ=255)

      aGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCaa                                                                                     <  1:866701/93‑1 (MQ=255)

      aGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCaa                                                                                     <  2:5721805/93‑1 (MQ=255)

      aGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCaa                                                                                     <  1:2035703/93‑1 (MQ=255)

       ggAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAAc                                                                                    <  2:62928/93‑1 (MQ=255)

        gagaGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAAcc                                                                                   <  1:5533600/93‑1 (MQ=255)

         agagCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCg                                                                                  >  2:157957/1‑93 (MQ=255)

          gagCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCgg                                                                                 >  2:9337300/1‑93 (MQ=255)

          gagCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCgg                                                                                 >  2:4945863/1‑93 (MQ=255)

          gagCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCgg                                                                                 >  1:3189939/1‑93 (MQ=255)

           agCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgt                                                                                <  1:1732417/93‑1 (MQ=255)

            gCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtt                                                                               >  2:1619680/1‑93 (MQ=255)

            gCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtt                                                                               >  2:588535/1‑93 (MQ=255)

            gCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtt                                                                               >  1:1232828/1‑93 (MQ=255)

            gCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtt                                                                               >  1:6904972/1‑93 (MQ=255)

            gCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtt                                                                               <  1:4684281/93‑1 (MQ=255)

               gTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtttgt                                                                            <  1:7760022/93‑1 (MQ=255)

               gTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtttgt                                                                            >  1:10010094/1‑93 (MQ=255)

                tttGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGGCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtttgtt                                                                           >  1:7504576/1‑93 (MQ=255)

                 ttgttgTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGgtttgttt                                                                          >  2:825828/1‑93 (MQ=255)

                 ttgttgTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGgtttgttt                                                                          >  2:9295792/1‑93 (MQ=255)

                  tgttgtTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGtttt                                                                         >  2:2184075/1‑93 (MQ=255)

                  tgttgtTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGtttt                                                                         >  2:7880486/1‑93 (MQ=255)

                   gttgttTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTc                                                                        >  2:9185075/1‑93 (MQ=255)

                     tgttTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCa                                                                      >  2:3268535/1‑93 (MQ=255)

                     tgttTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCa                                                                      <  2:5195958/93‑1 (MQ=255)

                     tgttTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCa                                                                      <  1:790868/93‑1 (MQ=255)

                         ttGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAgcgc                                                                  <  1:7104105/93‑1 (MQ=255)

                           gCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCaa                                                                >  1:9240293/1‑93 (MQ=255)

                            cAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCaaa                                                               <  2:4143747/93‑1 (MQ=255)

                            cAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCaaa                                                               <  1:48663/93‑1 (MQ=255)

                                tCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGcc                                                           >  1:1890091/1‑93 (MQ=255)

                                 ccaccaCCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGccc                                                          <  2:8599441/93‑1 (MQ=255)

                                 ccaccaCCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGccc                                                          <  2:8039037/93‑1 (MQ=255)

                                  caccacCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCa                                                         <  2:1682135/93‑1 (MQ=255)

                                   cccaccGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAg                                                        <  1:5508009/92‑1 (MQ=255)

                                     caccGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGAc                                                      <  2:6723667/93‑1 (MQ=255)

                                      cccGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACa                                                     <  1:9006966/92‑1 (MQ=255)

                                      accGCTTCGATTATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACa                                                     >  2:4479016/1‑93 (MQ=255)

                                        cGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGc                                                   >  1:957011/1‑93 (MQ=255)

                                         gCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGct                                                  >  2:3870403/1‑93 (MQ=255)

                                          cTTCGATGATATGTTTGCCGCCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGCTTTCCAGCGCAAACGCCCGGACAGctt                                                 >  2:354378/1‑93 (MQ=255)

                                           ttCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGcttc                                                <  1:9773102/93‑1 (MQ=255)

                                            tCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGcttct                                               >  1:6879812/1‑93 (MQ=255)

                                               atgatATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTa                                            <  2:6549554/93‑1 (MQ=255)

                                               atgatATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTa                                            >  2:8730578/1‑93 (MQ=255)

                                                tgatATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTAt                                           <  2:9561202/93‑1 (MQ=255)

                                                tgatATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTAt                                           <  2:2651419/93‑1 (MQ=255)

                                                 tatatGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATc                                          <  2:7568250/92‑1 (MQ=255)

                                                  atatGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCt                                         >  2:1805345/1‑93 (MQ=255)

                                                  atatGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCt                                         >  1:8205558/1‑93 (MQ=255)

                                                   tatGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTg                                        >  2:4276177/1‑93 (MQ=255)

                                                   tatGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTg                                        >  2:9037712/1‑93 (MQ=255)

                                                   tatGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTg                                        <  1:9126211/93‑1 (MQ=255)

                                                   taggTTTGCCGGCGCACATAATGACGTCTGCTTTGCGCGACTGGTCAACCGCTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTg                                        <  1:7031736/90‑1 (MQ=255)

                                                    atGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTgg                                       >  1:8525068/1‑93 (MQ=255)

                                                     tGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGc                                      >  1:6673012/1‑93 (MQ=255)

                                                      gTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCg                                     <  1:4491985/93‑1 (MQ=255)

                                                          gCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAg                                 <  1:5858955/93‑1 (MQ=255)

                                                          gCCGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAg                                 <  1:3259782/93‑1 (MQ=255)

                                                            cGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCa                               <  1:8319660/93‑1 (MQ=255)

                                                            cGTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCa                               >  1:6768728/1‑93 (MQ=255)

                                                             gTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAg                              >  2:2157250/1‑93 (MQ=255)

                                                             gTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAg                              <  1:2463624/93‑1 (MQ=255)

                                                             gTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAg                              <  1:644932/93‑1 (MQ=255)

                                                             gTCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAg                              >  1:9418050/1‑93 (MQ=255)

                                                              tCGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAgg                             <  2:3006759/93‑1 (MQ=255)

                                                              gcGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAgg                             <  1:5276288/92‑1 (MQ=255)

                                                               cGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAggg                            <  2:1528486/93‑1 (MQ=255)

                                                               cGAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAggg                            <  2:6366893/93‑1 (MQ=255)

                                                                gAGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGga                           <  2:2312155/93‑1 (MQ=255)

                                                                 aGCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGgag                          >  1:2992906/1‑93 (MQ=255)

                                                                  gCATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGgaga                         <  2:6873621/93‑1 (MQ=255)

                                                                   cATAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAt                        <  2:6460234/93‑1 (MQ=255)

                                                                    aTAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAtc                       >  1:3449358/1‑93 (MQ=255)

                                                                     tAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAtct                      >  2:6083122/1‑93 (MQ=255)

                                                                     tAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAtct                      >  2:9757053/1‑93 (MQ=255)

                                                                     tAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAtct                      >  1:9995697/1‑93 (MQ=255)

                                                                     tAATGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAtct                      >  1:9877088/1‑93 (MQ=255)

                                                                      aaTGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCGCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGAtctc                     <  1:3849254/93‑1 (MQ=255)

                                                                       aTGACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCa                    <  1:2425143/93‑1 (MQ=255)

                                                                        tGACGTCGGCTTTGCGCGAGTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAg                   >  2:1342218/1‑93 (MQ=255)

                                                                         gACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGt                  <  1:3754980/93‑1 (MQ=255)

                                                                         gACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGt                  <  2:735868/93‑1 (MQ=255)

                                                                         gACGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGt                  >  2:1064217/1‑93 (MQ=255)

                                                                           cGTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTaa                <  2:3150516/93‑1 (MQ=255)

                                                                            gTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTaaa               >  2:718503/1‑93 (MQ=255)

                                                                            gTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTaaa               >  2:3675100/1‑93 (MQ=255)

                                                                            gTCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTaaa               >  2:8388777/1‑93 (MQ=255)

                                                                             tCGGCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTaaaa              >  1:2253402/1‑93 (MQ=255)

                                                                               ggCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACg            <  1:1558014/93‑1 (MQ=255)

                                                                                gCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACgg           <  1:8925253/93‑1 (MQ=255)

                                                                                gCTTTGCGCGACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACgg           >  2:1931499/1‑93 (MQ=255)

                                                                                   ttGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTa        <  2:4830011/93‑1 (MQ=255)

                                                                                      cgcgACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTg     <  1:4300928/93‑1 (MQ=255)

                                                                                       gcgACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTgg    <  1:6217080/93‑1 (MQ=255)

                                                                                       gcgACTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTgg    <  1:2077705/93‑1 (MQ=255)

GTTGCCAGGAGAGCAGTTTGTTGTTTTGCAGATCCACCACCGCTTCGATGATATGTTTGCCGTCGAGCATAATGACGTCGGCTTTGCGCGGCTGGTCAACCGGTTTGTTTTCCAGCGCAAACGCCCAGACAGCTTCTTTATCTGGCGGTAGCAGGGAGATCTCAGTAAAACGGGTATTGGGT  >  NC_000913/1448749‑1448930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`