Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,464,084 T→C intergenic (+999/+1861) b1400 → / ← yi222 putative transferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,464,0840TC99.4% 1052.4 / ‑3.8 330intergenic (+999/+1861)b1400/yi222putative transferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (115/213);  total (117/213)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 343 total aligned reads displayed.

TAACACTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCA  >  NC_000913/1463994‑1464173
                                                                                          |                                                                                         
 aaCACTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGcc                                                                                        <  2:8880531/93‑1 (MQ=255)
 aaCACTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGGGATAACGcc                                                                                        >  1:6445387/1‑93 (MQ=255)
  acacTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCca                                                                                       >  1:5553738/1‑93 (MQ=255)
    acTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCcaca                                                                                     <  1:10028532/93‑1 (MQ=255)
    acTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCcaca                                                                                     <  2:9173454/93‑1 (MQ=255)
      tGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGt                                                                                   >  2:9017673/1‑93 (MQ=255)
      tGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGCGAGCTTGTTATTACTGGTGATAATTCcacact                                                                                   >  2:8397772/1‑91 (MQ=255)
      tGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGGGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGGGATAACGCCACAga                                                                                   >  1:2619435/1‑92 (MQ=255)
       gTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTa                                                                                  >  2:5863004/1‑93 (MQ=255)
          gCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAaac                                                                               >  2:4945546/1‑93 (MQ=255)
           cccTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAaaca                                                                              <  1:6930941/93‑1 (MQ=255)
            ccTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAaacaa                                                                             >  1:2105789/1‑93 (MQ=255)
               taaaGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAGcaacaa                                                                          <  2:4219198/91‑1 (MQ=255)
               tGAGGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAAcaacaa                                                                          <  2:4183703/93‑1 (MQ=255)
               tGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAAcaacaa                                                                          <  2:7272094/93‑1 (MQ=255)
                  aGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATgg                                                                       <  2:7960154/93‑1 (MQ=255)
                  aGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATgg                                                                       >  1:6757034/1‑93 (MQ=255)
                  aGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATgg                                                                       >  2:3572690/1‑93 (MQ=255)
                  aGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAAGCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATgg                                                                       <  2:2431791/93‑1 (MQ=255)
                    gggTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGAGATAACGCCACAGTAAACAACAATGGCa                                                                     >  1:1685189/1‑93 (MQ=255)
                     ggTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCaa                                                                    <  2:6263104/93‑1 (MQ=255)
                     ggTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCaa                                                                    >  1:8340344/1‑93 (MQ=255)
                      gTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCaaa                                                                   <  1:1480024/93‑1 (MQ=255)
                         aGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAacc                                                                <  2:2174605/93‑1 (MQ=255)
                          gcgcAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAacca                                                               <  1:4301622/93‑1 (MQ=255)
                          gcgcAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAacca                                                               >  1:9498778/1‑93 (MQ=255)
                          gcgcAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAacca                                                               <  2:9632601/93‑1 (MQ=255)
                           cgcAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAaccac                                                              <  1:926327/93‑1 (MQ=255)
                            gcAGAGCGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACCCCATAGTAAACAACAATGGCAAAaccacc                                                             <  2:5870299/93‑1 (MQ=255)
                             cAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCg                                                            <  1:4701030/93‑1 (MQ=255)
                               gATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGtt                                                          <  2:6560154/93‑1 (MQ=255)
                               gATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGtt                                                          >  1:5800774/1‑93 (MQ=255)
                               gATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGtt                                                          <  1:3820175/93‑1 (MQ=255)
                                aTGGCGCAACGAAGTGTCAATATAATCACAACGGTGAGCTTGTTATTACGGGTCATAACGCCACAGTAAACAACAATGGCAAAGCCACCGTTg                                                         <  2:7212815/93‑1 (MQ=255)
                                 tGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGa                                                        <  2:2356098/93‑1 (MQ=255)
                                 tGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGa                                                        <  1:7890866/93‑1 (MQ=255)
                                  ggCGCAACGAAGTGGCAATATAATCACAACGGTGAGTTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCGCCGTTGAc                                                       <  1:37610/93‑1 (MQ=255)
                                  ggCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGAc                                                       <  1:4596132/93‑1 (MQ=255)
                                    cgcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACgg                                                     <  2:7013701/93‑1 (MQ=255)
                                     gcAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGt                                                    >  2:4037547/1‑93 (MQ=255)
                                      cAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTa                                                   >  1:3449132/1‑93 (MQ=255)
                                       aaCGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTaa                                                  <  1:1380075/93‑1 (MQ=255)
                                        aCGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTaaa                                                 <  2:5155542/93‑1 (MQ=255)
                                         cGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAg                                                <  2:1629665/93‑1 (MQ=255)
                                         cGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAg                                                <  2:3288514/93‑1 (MQ=255)
                                          gAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCCCAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGa                                               >  2:2113856/1‑93 (MQ=255)
                                          gAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGa                                               >  1:9956555/1‑93 (MQ=255)
                                          gAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGa                                               >  2:3116952/1‑93 (MQ=255)
                                           aaGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGAt                                              <  2:7613188/93‑1 (MQ=255)
                                           aaGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGAt                                              >  2:1252650/1‑93 (MQ=255)
                                             gTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTc                                            <  2:2604872/93‑1 (MQ=255)
                                             gTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTc                                            <  1:9450782/93‑1 (MQ=255)
                                              tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTcc                                           <  1:8941248/93‑1 (MQ=255)
                                              tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTcc                                           <  1:8173303/93‑1 (MQ=255)
                                              tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTcc                                           <  1:7170912/93‑1 (MQ=255)
                                              tGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTcc                                           >  2:5948503/1‑93 (MQ=255)
                                               ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCa                                          <  2:4623059/93‑1 (MQ=255)
                                               ggCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCa                                          <  2:8478984/93‑1 (MQ=255)
                                                 cAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCAcc                                        <  1:2653424/93‑1 (MQ=255)
                                                   atatAATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCgg                                      >  1:4525570/1‑93 (MQ=255)
                                                    tataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGt                                     <  2:5591794/93‑1 (MQ=255)
                                                    tataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGt                                     >  1:252027/1‑93 (MQ=255)
                                                     ataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTa                                    >  2:8126349/1‑93 (MQ=255)
                                                     ataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTa                                    <  1:1301537/93‑1 (MQ=255)
                                                     ataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTa                                    >  1:4029221/1‑93 (MQ=255)
                                                     ataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTa                                    <  1:4329247/93‑1 (MQ=255)
                                                     ataATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTa                                    >  2:386793/1‑93 (MQ=255)
                                                      taATCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTAc                                   <  1:6328368/93‑1 (MQ=255)
                                                       aaTCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACg                                  <  1:6127349/93‑1 (MQ=255)
                                                       aaTCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACg                                  >  2:1371120/1‑93 (MQ=255)
                                                        aTCACAACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACgg                                 <  2:7138325/93‑1 (MQ=255)
                                                           acaACGGTGTGCTTGTTTTTACGGGTGATAACGCAACAGTAGACAACCATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGaag                              <  2:8983768/93‑2 (MQ=21)
                                                           acaACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGaaa                              <  1:1147226/93‑1 (MQ=255)
                                                           acaACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGaaa                              >  2:1526817/1‑93 (MQ=255)
                                                            caACGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAAt                             <  2:3762804/93‑1 (MQ=255)
                                                             aaCGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATc                            <  2:9222378/93‑1 (MQ=255)
                                                             aaCGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATc                            >  1:75326/1‑93 (MQ=255)
                                                              aCGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCa                           <  1:7657740/93‑1 (MQ=255)
                                                              aCGGTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCa                           >  1:8700054/1‑93 (MQ=255)
                                                                ggTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAAc                         <  1:5304546/93‑1 (MQ=255)
                                                                ggTGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAAc                         <  1:5955131/93‑1 (MQ=255)
                                                                  tGAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACgg                       >  2:5424396/1‑93 (MQ=255)
                                                                   gAGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGt                      >  2:9841113/1‑93 (MQ=255)
                                                                    aGCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGta                     <  1:9168155/93‑1 (MQ=255)
                                                                     gCTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGtaa                    <  1:4891925/93‑1 (MQ=255)
                                                                      cTTGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGtaat                   >  2:1142477/1‑93 (MQ=255)
                                                                       ttGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGtaata                  >  1:6042011/1‑93 (MQ=255)
                                                                        tGTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGtaataa                 <  2:613892/93‑1 (MQ=255)
                                                                         gTTATTACGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAAc                <  2:2972260/93‑1 (MQ=255)
                                                                              taCGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGaa           <  1:9713845/93‑1 (MQ=255)
                                                                               aCGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGaaa          >  1:4662468/1‑93 (MQ=255)
                                                                               aCGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGaaa          <  2:5746486/93‑1 (MQ=255)
                                                                                cGGGTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAg         <  2:4325345/93‑1 (MQ=255)
                                                                                 gggTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGt        <  1:304799/93‑1 (MQ=255)
                                                                                   gTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGa      <  1:3514724/93‑1 (MQ=255)
                                                                                   gTGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGa      <  1:3351569/93‑1 (MQ=255)
                                                                                   gTGATAACGCCACAGTAAACAAAAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGa      >  2:6489818/1‑93 (MQ=255)
                                                                                    tGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGAt     >  1:512197/1‑93 (MQ=255)
                                                                                    tGATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGAt     <  2:7434299/93‑1 (MQ=255)
                                                                                    ggATAACGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGAt     <  2:8574567/92‑1 (MQ=255)
                                                                                          |                                                                                         
TAACACTGTTGCCCTTGAAGGGGTGAGCGCAGATGGCGCAACGAAGTGGCAATATAATCACAACGGTGAGCTTGTTATTACGGGTGATAATGCCACAGTAAACAACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCA  >  NC_000913/1463994‑1464173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑