BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,477,747	A→G	I196V (ATT→GTT)	b1410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,477,747	0	A	G	91.4%	611.7 / 39.6	233	I196V (ATT→GTT)	b1410	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (11/9); new base G (126/87); total (137/96)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 238 total aligned reads displayed.

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

 ttattaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTg                                                                                        >  1:1368872/1‑93 (MQ=255)

  tattaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGc                                                                                       <  2:2134826/93‑1 (MQ=255)

   attaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGcc                                                                                      <  1:6569581/93‑1 (MQ=255)

     taATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGt                                                                                    >  2:4592837/1‑93 (MQ=255)

        ttCCGATGTGAAAGGCTTATAGTTGACCCAGTATCGGCAGCGGGGGGGGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTaa                                                                                 <  2:6421594/93‑1 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               <  2:9040437/93‑1 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               >  1:4611388/1‑93 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               >  2:5758917/1‑93 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               <  1:6582566/93‑1 (MQ=255)

           cTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAatat                                                                              <  1:8516030/93‑1 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  2:9446632/1‑93 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             <  1:6326495/93‑1 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  2:198265/1‑93 (MQ=255)

              tgtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCtt                                                                           >  2:4833591/1‑93 (MQ=255)

               gtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTg                                                                          >  1:784879/1‑93 (MQ=255)

                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         <  2:4385811/93‑1 (MQ=255)

                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         >  2:6966874/1‑93 (MQ=255)

                  aaaGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTc                                                                       <  2:592959/93‑1 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     >  1:8857547/1‑93 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     <  1:5030260/93‑1 (MQ=255)

                     ggACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    <  1:6464655/93‑1 (MQ=255)

                     ggACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    >  1:5124802/1‑93 (MQ=255)

                     ggACGCTATTTGACCCACAAGCGGCACCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    <  1:5338936/93‑1 (MQ=255)

                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGGGAATATCTTGCTCGAtc                                                                   >  1:8970705/1‑93 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 >  1:7908920/1‑93 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 <  2:7823803/93‑1 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                >  1:911141/1‑93 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                <  1:9580801/93‑1 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATctct                                                                >  1:1729805/1‑93 (MQ=255)

                          cTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTa                                                               >  1:9391049/1‑93 (MQ=255)

                           tATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAc                                                              >  1:7116285/1‑93 (MQ=255)

                           tATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAc                                                              >  1:2154851/1‑93 (MQ=255)

                            aTTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACa                                                             >  1:8871425/1‑93 (MQ=255)

                               tGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                          <  1:9574062/93‑1 (MQ=255)

                               tGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                          <  1:1611528/93‑1 (MQ=255)

                                gACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAc                                                         >  2:8609623/1‑93 (MQ=255)

                                  cccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGt                                                       >  1:5317952/1‑93 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTCAAAAACGTc                                                      >  1:2031957/1‑93 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                      <  2:6461367/93‑1 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                      <  2:2022229/93‑1 (MQ=255)

                                    cACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCt                                                     <  1:4206296/93‑1 (MQ=255)

                                      cGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGa                                                   <  2:1260651/93‑1 (MQ=255)

                                      cGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGa                                                   <  1:9765144/93‑1 (MQ=255)

                                        aTCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTCACCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAc                                                 >  1:849322/1‑93 (MQ=255)

                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                >  2:3489485/1‑93 (MQ=255)

                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                >  2:5224123/1‑93 (MQ=255)

                                           ggCAACGGGGGGCGAGTTTCAATAATTATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGta                                              <  1:1519927/93‑1 (MQ=255)

                                           ggCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGta                                              >  2:247563/1‑93 (MQ=255)

                                             cAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtatt                                            <  2:7451984/93‑1 (MQ=255)

                                               aCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtattat                                          <  2:1045695/93‑1 (MQ=255)

                                                cGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTattatt                                         <  1:9946797/93‑1 (MQ=255)

                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  1:2416865/93‑1 (MQ=255)

                                                   ggggCGAGTTTCAATAATGATCCGCTGATCCCACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTaga                                      <  1:3146602/93‑1 (MQ=255)

                                                   ggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTaga                                      >  1:6175419/1‑93 (MQ=255)

                                                    gggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTagag                                     <  1:2192356/93‑1 (MQ=255)

                                                    gggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTagag                                     <  1:804650/93‑1 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    >  2:3039717/1‑93 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    <  2:3258249/93‑1 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    >  1:1993536/1‑93 (MQ=255)

                                                     ggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAgaga                                    <  1:3959433/93‑1 (MQ=255)

                                                       cGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCAACAAAACGTCTGAACGTATTATTAGGGATg                                  <  2:7684076/93‑1 (MQ=255)

                                                        gAGTTTCAATAATGAACCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 <  2:7007995/93‑1 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggg                              >  1:2243468/1‑92 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              >  2:6118692/1‑93 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              >  1:8233258/1‑93 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              <  2:976729/93‑1 (MQ=255)

                                                            ttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcg                             <  2:9856857/93‑1 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  2:6561284/1‑93 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  1:8762862/1‑93 (MQ=255)

                                                                  aatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAtt                       >  2:3976889/1‑93 (MQ=255)

                                                                  aatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAtt                       >  2:7932655/1‑93 (MQ=255)

                                                                   atGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTa                      <  2:287445/93‑1 (MQ=255)

                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     >  1:9333487/1‑93 (MQ=255)

                                                                     gATCCGCTGATCAGACGGGCGGTTTCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    <  1:9275665/93‑1 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTagc                    >  1:7287303/1‑91 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    <  1:2759676/93‑1 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    <  2:6092571/93‑1 (MQ=255)

                                                                       tCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCt                  <  1:8815120/93‑1 (MQ=255)

                                                                       tCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCt                  <  1:7394509/93‑1 (MQ=255)

                                                                        ccGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTc                 >  1:8302616/1‑93 (MQ=255)

                                                                         cGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTCTTATTAGAGATGCGGCGGCGATTACGCTcc                >  1:3653961/1‑93 (MQ=255)

                                                                          gCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc               >  1:3214662/1‑93 (MQ=255)

                                                                          gCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc               >  2:5084056/1‑93 (MQ=255)

                                                                          gCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccc               >  2:1743755/1‑93 (MQ=255)

                                                                           cTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTcccc              <  1:6079136/93‑1 (MQ=255)

                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTccccc             >  2:7888568/1‑92 (MQ=255)

                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCa             >  1:5411646/1‑93 (MQ=255)

                                                                             gATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGGACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCAc            <  2:8783144/93‑1 (MQ=255)

                                                                             gATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCAc            >  1:2649211/1‑93 (MQ=255)

                                                                              aTCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACg           >  2:2656732/1‑93 (MQ=255)

                                                                              aTCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACg           >  2:2411095/1‑93 (MQ=255)

                                                                                 acacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCaa        >  2:2925178/1‑93 (MQ=255)

                                                                                  cacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAc       >  2:5464930/1‑93 (MQ=255)

                                                                                  cacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAc       >  1:1796795/1‑93 (MQ=255)

                                                                                  cacGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAAc       >  2:9152940/1‑93 (MQ=255)

                                                                                   acGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACt      >  1:5859718/1‑93 (MQ=255)

                                                                                    cGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt     >  2:389768/1‑93 (MQ=255)

                                                                                    cGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt     >  1:1082053/1‑93 (MQ=255)

                                                                                    cGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACtt     >  2:426064/1‑93 (MQ=255)

                                                                                       gCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTg  <  2:8433269/93‑1 (MQ=255)

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`