BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	143,862	A→G	H54R (CAT→CGT)	yadH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	143,862	0	A	G	99.5%	1343.1 / ‑3.8	415	H54R (CAT→CGT)	yadH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (212/200); total (213/202)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 426 total aligned reads displayed.

GGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTT  >  NC_000913/143772‑143951

gggTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTg                                                                                         <  2:3266930/93‑1 (MQ=255)

 ggTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTgg                                                                                        <  1:4700146/93‑1 (MQ=255)

  gTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGc                                                                                       <  2:2845861/93‑1 (MQ=255)

  gTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGc                                                                                       >  1:6888312/1‑93 (MQ=255)

   tGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCt                                                                                      >  2:3504143/1‑93 (MQ=255)

    gCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCtt                                                                                     >  1:5582673/1‑93 (MQ=255)

     cAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTc                                                                                    <  2:8673393/93‑1 (MQ=255)

      aGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCa                                                                                   <  1:7380184/93‑1 (MQ=255)

      aGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCGTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCa                                                                                   <  1:4427625/93‑1 (MQ=255)

       gACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAg                                                                                  >  2:1960286/1‑93 (MQ=255)

       gACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAg                                                                                  <  1:5905266/93‑1 (MQ=255)

       gACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAg                                                                                  >  2:9237133/1‑93 (MQ=255)

         cGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCt                                                                                <  1:8833173/93‑1 (MQ=255)

           cTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCtat                                                                              <  2:1603818/93‑1 (MQ=255)

            cggTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCtata                                                                             <  1:2481515/92‑1 (MQ=255)

               tGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGc                                                                          <  1:5206037/93‑1 (MQ=255)

                    ccAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTc                                                                     <  1:716638/93‑1 (MQ=255)

                    ccAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTc                                                                     >  1:4117316/1‑93 (MQ=255)

                      aGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCAt                                                                   >  2:6846326/1‑93 (MQ=255)

                      aGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCAt                                                                   >  2:8429496/1‑93 (MQ=255)

                       gTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATc                                                                  >  1:2182603/1‑93 (MQ=255)

                       gTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATc                                                                  >  1:8326860/1‑93 (MQ=255)

                       gTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATc                                                                  <  1:9929145/93‑1 (MQ=255)

                         catcaCCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGt                                                                <  2:7334294/93‑1 (MQ=255)

                          atcaCCATGACCGTTTACTTCATTATCTTAGGTAACCTGATTGGTTCGCGCATTGGCCATATGCCTGGCTTAAGCTATATGCAGTTCATCGTa                                                               <  1:7682938/93‑1 (MQ=255)

                          atcaCCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTa                                                               <  2:141398/93‑1 (MQ=255)

                           tcaCCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTAc                                                              >  2:234033/1‑93 (MQ=255)

                           tcaCCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTAc                                                              <  2:7160035/93‑1 (MQ=255)

                           tcaCCATGACCCTTTACTTTATGATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTAc                                                              >  2:6405886/1‑93 (MQ=255)

                           tcaCCATGACCCTTTACCTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTAc                                                              >  1:6659130/1‑93 (MQ=255)

                             aCCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCg                                                            >  2:2620612/1‑93 (MQ=255)

                                aTGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCgggg                                                         >  1:1788780/1‑93 (MQ=255)

                                aTGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCgggg                                                         >  1:1708270/1‑93 (MQ=255)

                                 tGACCCTTTTCTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGc                                                        <  1:3464647/93‑1 (MQ=255)

                                 tGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGc                                                        >  1:32095/1‑93 (MQ=255)

                                 tGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGc                                                        >  1:2038235/1‑93 (MQ=255)

                                 tGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGc                                                        >  2:6917253/1‑93 (MQ=255)

                                  gACTCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCt                                                       <  1:7952997/93‑1 (MQ=255)

                                    cccTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGTTATATGCAGTTCATCGTACCGGGGCTGa                                                     <  2:9334742/93‑1 (MQ=255)

                                    cccTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGa                                                     <  1:6480847/93‑1 (MQ=255)

                                    cccTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGa                                                     >  2:3910315/1‑93 (MQ=255)

                                     ccTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGTGATTTGCGTGGCTTCAGCTATATGCAGCTCATCGTACCGGGGCTGAt                                                    >  2:8970735/1‑93 (MQ=255)

                                     ccTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGAt                                                    <  1:2397319/93‑1 (MQ=255)

                                     ccTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGAt                                                    <  1:5411521/93‑1 (MQ=255)

                                      ctttactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATc                                                   >  1:9401983/1‑93 (MQ=255)

                                      ctttactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATc                                                   <  1:4309904/93‑1 (MQ=255)

                                       tttactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCa                                                  >  1:3937969/1‑93 (MQ=255)

                                       tttactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCa                                                  >  1:124898/1‑93 (MQ=255)

                                       tttactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCa                                                  >  2:4271970/1‑93 (MQ=255)

                                         tactttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatg                                                <  2:1374070/93‑1 (MQ=255)

                                          tctttaTTATCTTCGGTAACCTGCTTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatga                                               <  2:5590140/92‑1 (MQ=255)

                                          actttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatga                                               <  2:6223826/93‑1 (MQ=255)

                                          actttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatga                                               <  2:47015/93‑1 (MQ=255)

                                          actttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatga                                               <  1:10008265/93‑1 (MQ=255)

                                          actttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatga                                               >  1:496487/1‑93 (MQ=255)

                                           ctttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatgat                                              <  2:3657744/93‑1 (MQ=255)

                                            tttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatgatg                                             >  2:5014306/1‑93 (MQ=255)

                                            tttaTTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCatgatg                                             >  2:879306/1‑93 (MQ=255)

                                             ttattaTCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGt                                            <  1:1919777/93‑1 (MQ=255)

                                             ttattaTCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGt                                            <  1:3106814/93‑1 (MQ=255)

                                             ttattaTCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGt                                            <  1:6050892/93‑1 (MQ=255)

                                              tattatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGTGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTc                                           <  2:9656116/93‑1 (MQ=255)

                                               attatCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCg                                          <  1:8612671/93‑1 (MQ=255)

                                                   tCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGa                                      >  1:4880468/1‑93 (MQ=255)

                                                    cTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGAt                                     <  1:1130383/93‑1 (MQ=255)

                                                     ttCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATc                                    <  1:5112552/93‑1 (MQ=255)

                                                        ggTAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCAcc                                 >  2:9925857/1‑93 (MQ=255)

                                                          tAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCaa                               >  1:3824542/1‑93 (MQ=255)

                                                          tAACCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCaa                               <  2:8301506/93‑1 (MQ=255)

                                                           aaCCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAAt                              >  2:4180338/1‑93 (MQ=255)

                                                           aaCCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAAt                              >  2:10044571/1‑93 (MQ=255)

                                                            aCCTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGCCGGTGATCACCACTg                             >  2:787024/1‑93 (MQ=255)

                                                             ccTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGc                            <  1:9033837/93‑1 (MQ=255)

                                                              cTGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGcc                           >  1:4546787/1‑93 (MQ=255)

                                                               tGATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCt                          <  1:9805554/93‑1 (MQ=255)

                                                                gATTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTa                         >  2:4556763/1‑93 (MQ=255)

                                                                 aTTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTAc                        <  2:2987548/93‑1 (MQ=255)

                                                                 aTTGGTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTAc                        <  2:5487243/93‑1 (MQ=255)

                                                                    ggTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGcc                     >  2:7053470/1‑93 (MQ=255)

                                                                     gTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCa                    >  1:8230812/1‑93 (MQ=255)

                                                                     gTTCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCa                    >  1:7440200/1‑93 (MQ=255)

                                                                      ttCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCaa                   >  2:6722127/1‑93 (MQ=255)

                                                                      ttCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCaa                   <  2:2150463/93‑1 (MQ=255)

                                                                       tCGCGTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAAc                  >  2:1175014/1‑93 (MQ=255)

                                                                         gcgTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGt                <  1:7823925/93‑1 (MQ=255)

                                                                         gcgTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGt                <  2:7546802/93‑1 (MQ=255)

                                                                         gcgTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGt                <  1:2920995/93‑1 (MQ=255)

                                                                         gcgTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGt                >  1:1541997/1‑93 (MQ=255)

                                                                         gcgTATTGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGt                >  1:1368760/1‑93 (MQ=255)

                                                                              ttGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGc                            <  2:5346971/76‑1 (MQ=255)

                                                                               tGGCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGtc          >  1:1473171/1‑93 (MQ=255)

                                                                                 gCGATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGtcat        >  1:3503445/1‑93 (MQ=255)

                                                                                   gATATGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGtcatca      >  1:9285793/1‑93 (MQ=255)

                                                                                    atatGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTcatcat     <  1:6363338/93‑1 (MQ=255)

                                                                                    atatGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTcatcat     >  1:8772815/1‑93 (MQ=255)

                                                                                    atatGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTcatcat     >  1:2699483/1‑93 (MQ=255)

                                                                                     tatGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAtt    >  1:9623915/1‑93 (MQ=255)

                                                                                     tatGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAtt    >  1:1870022/1‑93 (MQ=255)

                                                                                      atGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAttt   <  1:6156066/93‑1 (MQ=255)

                                                                                       tGCGTGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCAtttt  >  1:9753757/1‑93 (MQ=255)

GGGTGCAGACGCTGGTGCCGCCAGTCATCACCATGACCCTTTACTTTATTATCTTCGGTAACCTGATTGGTTCGCGTATTGGCGATATGCATGGCTTCAGCTATATGCAGTTCATCGTACCGGGGCTGATCATGATGTCGGTGATCACCAATGCCTACGCCAACGTTGCGTCATCATTTT  >  NC_000913/143772‑143951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`