BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,478,094	A→G	G311G (GGA→GGG)	b1410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,094	0	A	G	99.6%	805.9 / NA	261	G311G (GGA→GGG)	b1410	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (130/130); total (130/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

TTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT  >  NC_000913/1478004‑1478183

   atatGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGt                                                                                      >  2:6237751/1‑93 (MQ=255)

   atatGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGt                                                                                      <  2:8675882/93‑1 (MQ=255)

        gtAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCAt                                                                                 <  2:8814446/92‑1 (MQ=255)

          aaCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTa                                                                               <  1:2364050/93‑1 (MQ=255)

          aaCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTa                                                                               <  2:6925319/93‑1 (MQ=255)

           aCAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTAt                                                                              <  2:1778065/93‑1 (MQ=255)

            cAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATc                                                                             <  2:8173989/93‑1 (MQ=255)

              aaTCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGc                                                                           <  2:428192/93‑1 (MQ=255)

                tCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCg                                                                         <  2:2840894/93‑1 (MQ=255)

                tCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCg                                                                         <  1:3749331/93‑1 (MQ=255)

                tCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCg                                                                         >  1:7606139/1‑93 (MQ=255)

                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       >  2:9291904/1‑93 (MQ=255)

                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       <  2:7863032/93‑1 (MQ=255)

                  gCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGt                                                                       <  1:6383747/93‑1 (MQ=255)

                   cAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGTCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGtt                                                                      <  1:5331651/93‑1 (MQ=255)

                   cAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGtt                                                                      <  1:1908230/93‑1 (MQ=255)

                     aaaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGa                                                                    <  2:1855846/93‑1 (MQ=255)

                      aaaTTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGAt                                                                   <  2:9467360/93‑1 (MQ=255)

                         tttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTa                                                                >  2:2262689/1‑93 (MQ=255)

                          ttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAg                                                               >  1:2144382/1‑93 (MQ=255)

                          ttGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAg                                                               <  2:5411645/93‑1 (MQ=255)

                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             <  2:4003584/93‑1 (MQ=255)

                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             <  2:5099465/93‑1 (MQ=255)

                            gATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCg                                                             <  2:1729805/93‑1 (MQ=255)

                             aTTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGt                                                            <  2:3473111/93‑1 (MQ=255)

                              ttATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTa                                                           <  1:6724965/93‑1 (MQ=255)

                               tATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGACCGTGCCAGTATCGCCGGCTGATTTAGCGTAt                                                          >  1:5913864/1‑93 (MQ=255)

                               tATCAGCATGAAGACTGCACAGGAACATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTAt                                                          <  1:8980228/93‑1 (MQ=255)

                                aTCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATc                                                         <  2:361849/93‑1 (MQ=255)

                                 tCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCg                                                        <  2:2041934/93‑1 (MQ=255)

                                   aGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCt                                                      <  1:9832667/93‑1 (MQ=255)

                                   aGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCt                                                      >  2:6109645/1‑93 (MQ=255)

                                     cATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCttt                                                    <  2:6764324/93‑1 (MQ=255)

                                     cATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCttt                                                    <  2:8053203/93‑1 (MQ=255)

                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGGCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCcttt                                                   >  2:8329911/1‑92 (MQ=255)

                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTa                                                   >  2:1267429/1‑93 (MQ=255)

                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTa                                                   >  1:7917092/1‑93 (MQ=255)

                                      aTGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTa                                                   <  2:1993536/93‑1 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGGTTTAGCGTATCGCTTTAt                                                  >  2:2290129/1‑93 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  >  1:610833/1‑93 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  >  1:337666/1‑93 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTAt                                                  <  1:8255615/93‑1 (MQ=255)

                                       tGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGCTGATTTAGCGTATCGCTTTAt                                                  >  1:10006018/1‑93 (MQ=255)

                                        gAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATg                                                 <  1:408009/93‑1 (MQ=255)

                                         aaGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGc                                                >  2:4467354/1‑93 (MQ=255)

                                          aGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCg                                               <  1:2920935/93‑1 (MQ=255)

                                          aGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCg                                               <  1:6278130/93‑1 (MQ=255)

                                           gACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGa                                              >  1:8751098/1‑93 (MQ=255)

                                            aCTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGaa                                             <  1:3039717/93‑1 (MQ=255)

                                              tGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                           >  2:5727017/1‑93 (MQ=255)

                                              tGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAg                                           >  2:7582161/1‑93 (MQ=255)

                                               gCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAgg                                          >  2:2906856/1‑93 (MQ=255)

                                                cacaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGc                                         <  2:6140391/93‑1 (MQ=255)

                                                cacaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGc                                         <  2:8531978/93‑1 (MQ=255)

                                                 acaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCg                                        >  2:7121049/1‑93 (MQ=255)

                                                 acaGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATCATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCg                                        >  2:3335611/1‑93 (MQ=255)

                                                  caGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGCTGATTTAGCGTATCGCTTTATGCGAAAGGCGa                                       >  2:1234809/1‑93 (MQ=255)

                                                   aGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGAt                                      <  2:2517007/93‑1 (MQ=255)

                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  1:3235475/1‑93 (MQ=255)

                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     <  2:7921192/93‑1 (MQ=255)

                                                    ggACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  1:4712923/1‑93 (MQ=255)

                                                    ggACCATCAGCGGATCGATGGCGGATACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATg                                     >  2:7278580/1‑93 (MQ=255)

                                                     gACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGa                                    <  2:6395895/93‑1 (MQ=255)

                                                       ccATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGaaa                                  >  2:9551331/1‑93 (MQ=255)

                                                       ccATCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGaaa                                  <  1:4031614/93‑1 (MQ=255)

                                                         aTCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAtt                                <  1:1373517/93‑1 (MQ=255)

                                                         aTCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAAtt                                >  2:5699761/1‑93 (MQ=255)

                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               <  1:5617186/93‑1 (MQ=255)

                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               >  2:5382859/1‑93 (MQ=255)

                                                          tCAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTg                               <  1:8677751/93‑1 (MQ=255)

                                                           cAGCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGt                              >  1:4504944/1‑93 (MQ=255)

                                                             gCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTc                            >  2:2086529/1‑93 (MQ=255)

                                                             gCGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTc                            <  2:9051613/93‑1 (MQ=255)

                                                              cGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCTATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCg                           <  2:9261078/93‑1 (MQ=255)

                                                              cGGATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGGAATTGTTCg                           >  1:3951724/1‑93 (MQ=255)

                                                               ggATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCgg                          >  1:1419498/1‑93 (MQ=255)

                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         <  2:8293049/93‑1 (MQ=255)

                                                                gATCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCggg                         >  1:1666522/1‑93 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        >  1:856565/1‑93 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        <  1:2691956/93‑1 (MQ=255)

                                                                 aTCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGa                        <  1:3585047/93‑1 (MQ=255)

                                                                  tCGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGAc                       <  2:2647359/93‑1 (MQ=255)

                                                                   cGATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCgggcag                      >  1:1372706/1‑90 (MQ=255)

                                                                    gATGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGc                     >  1:5394623/1‑93 (MQ=255)

                                                                      tGGCGGCTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCAc                   <  2:1413100/93‑1 (MQ=255)

                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATTAAATTGTTCGGGACGCACTCTTc              >  2:5540950/1‑93 (MQ=255)

                                                                           gcTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTc              >  1:9452165/1‑93 (MQ=255)

                                                                            cgaCTTGCTGGTGGTCCCGTGCCATTATCGCCGCTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCg             <  2:1056107/91‑1 (MQ=255)

                                                                            cTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCg             >  1:1095230/1‑93 (MQ=255)

                                                                            cTACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCg             <  1:704932/93‑1 (MQ=255)

                                                                             tACTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCgg            <  1:4659978/93‑1 (MQ=255)

                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          <  1:5074272/93‑1 (MQ=255)

                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          >  1:2045483/1‑93 (MQ=255)

                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          <  2:5009566/93‑1 (MQ=255)

                                                                               cTTTCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGc          <  2:4446313/93‑1 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         <  1:8042550/93‑1 (MQ=255)

                                                                                tttCTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGcc         >  2:4706753/1‑93 (MQ=255)

                                                                                   cTGGTGGGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGc      >  2:410777/1‑93 (MQ=255)

                                                                                      gtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCAtt   >  1:1286268/1‑92 (MQ=255)

                                                                                      gtggGCCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGAACTCTTCGGGCCTGCAtc   >  2:3585369/1‑93 (MQ=255)

TTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGGATCGATGGCGGCTACTTTCTGGTGGACCCGTGCCATTATCGCCGGTTGATTTAGCGTATCGCTTTATGCGAAAGGCGATGAAATTGTTCGGGACGCACTCTTCGGGCCTGCATCT  >  NC_000913/1478004‑1478183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`