BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,561,521	C→T	S754N (AGC→AAC)	b1489 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,561,521	0	C	T	99.6%	915.1 / ‑6.0	260	S754N (AGC→AAC)	b1489	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (123/135); total (125/135)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 273 total aligned reads displayed.

ATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGCTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAATTTTGATT  >  NC_000913/1561430‑1561610

 tatCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGttt                                                                                         <  2:9035635/93‑1 (MQ=255)

 tatCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGttt                                                                                         >  2:4201797/1‑93 (MQ=255)

 tatCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGttt                                                                                         <  1:6469058/93‑1 (MQ=255)

 tatCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGttt                                                                                         <  2:4029194/93‑1 (MQ=255)

 tatCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGttt                                                                                         >  1:3658560/1‑93 (MQ=255)

    cccTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGc                                                                                      <  2:5453815/93‑1 (MQ=255)

      cTGAATAACGCTACAGCGGATCCTGCGTAGCATCTCAAATTGCTCATTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGccc                                                                                    <  2:917001/93‑1 (MQ=255)

       tGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCa                                                                                   >  1:1426962/1‑93 (MQ=255)

        gAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCaa                                                                                  <  1:2702449/93‑1 (MQ=255)

        gAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCaa                                                                                  <  2:3962580/93‑1 (MQ=255)

         aaTAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAAt                                                                                 <  1:3778207/93‑1 (MQ=255)

         aaTAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAAt                                                                                 <  1:4939715/93‑1 (MQ=255)

         aaTAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAAt                                                                                 <  1:5269516/93‑1 (MQ=255)

           tAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATgt                                                                               >  1:3139308/1‑92 (MQ=255)

           tAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGc                                                                               >  1:6012674/1‑93 (MQ=255)

            aaCGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCt                                                                              <  1:7117959/93‑1 (MQ=255)

                  aCAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAAt                                                                        <  1:6561645/93‑1 (MQ=255)

                  aCAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAAt                                                                        <  2:9669105/93‑1 (MQ=255)

                  aCAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAAt                                                                        <  2:6974507/93‑1 (MQ=255)

                   cAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATg                                                                       <  2:7456318/93‑1 (MQ=255)

                    aGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATgg                                                                      <  2:1190041/93‑1 (MQ=255)

                     gTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGc                                                                     >  1:5779886/1‑93 (MQ=255)

                      tGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCt                                                                    >  1:1528160/1‑93 (MQ=255)

                       ggATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCtt                                                                   >  1:1776387/1‑93 (MQ=255)

                         aTCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCa                                                                 >  2:2189044/1‑93 (MQ=255)

                           cTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAg                                                               <  2:6259231/93‑1 (MQ=255)

                           cTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAg                                                               >  1:2419072/1‑93 (MQ=255)

                           cTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAg                                                               >  1:6233438/1‑93 (MQ=255)

                           cTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAg                                                               <  1:5573623/93‑1 (MQ=255)

                            ttGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGt                                                              >  2:7015893/1‑93 (MQ=255)

                              tcGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTaa                                                            <  1:1165230/92‑1 (MQ=255)

                              gCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTaa                                                            <  2:845477/93‑1 (MQ=255)

                              gCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTaa                                                            <  1:382182/93‑1 (MQ=255)

                                gTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGTGGGTTTGCCCAATGCTGGTAATGGCTGTAAGTAAgg                                                          >  2:8946700/1‑93 (MQ=255)

                                gTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAgg                                                          >  1:288790/1‑93 (MQ=255)

                                gTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAgg                                                          >  1:8820621/1‑93 (MQ=255)

                                 tAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGc                                                         >  2:4230347/1‑93 (MQ=255)

                                 tAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGc                                                         >  1:9524464/1‑93 (MQ=255)

                                 tAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGc                                                         >  1:1729005/1‑93 (MQ=255)

                                 tAGCATCTCAAATTGCCCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGc                                                         <  2:7828473/93‑1 (MQ=255)

                                   gCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCaa                                                       <  1:5529945/93‑1 (MQ=255)

                                   gCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCaa                                                       >  1:2196407/1‑93 (MQ=255)

                                    cATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAg                                                      <  2:3669886/93‑1 (MQ=255)

                                     aTCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAgg                                                     >  1:3391136/1‑93 (MQ=255)

                                      tctcAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGa                                                    >  2:7132468/1‑93 (MQ=255)

                                        tcAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATg                                                  <  2:5726870/93‑1 (MQ=255)

                                          aaaTTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCg                                                <  2:1994812/93‑1 (MQ=255)

                                           aaTTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGt                                               >  2:9283629/1‑93 (MQ=255)

                                           aaTTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGCTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGt                                               >  2:9487654/1‑93 (MQ=255)

                                            aTTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGtt                                              <  2:1582186/93‑1 (MQ=255)

                                              tGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGtttt                                            >  2:8637804/1‑93 (MQ=255)

                                                ttctTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTc                                          <  2:80538/92‑1 (MQ=255)

                                                ctctTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTc                                          <  1:3324346/93‑1 (MQ=255)

                                                  ctTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCgg                                        <  2:5277627/93‑1 (MQ=255)

                                                  ctTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCgg                                        >  1:8074381/1‑93 (MQ=255)

                                                   tttGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGt                                       >  1:8277674/1‑93 (MQ=255)

                                                       gTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGa                                   <  2:2798224/93‑1 (MQ=255)

                                                        tttCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAgcc                                  >  2:1229560/1‑91 (MQ=255)

                                                        tttCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGAc                                  <  1:3449974/93‑1 (MQ=255)

                                                          tCGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACaa                                <  2:770079/93‑1 (MQ=255)

                                                           cGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAAc                               <  2:2833266/93‑1 (MQ=255)

                                                           cGACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAAc                               <  2:4573262/93‑1 (MQ=255)

                                                            gACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACg                              <  1:1595966/93‑1 (MQ=255)

                                                            gACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACg                              >  2:6400321/1‑93 (MQ=255)

                                                            gACGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACg                              <  1:7868595/93‑1 (MQ=255)

                                                              cGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGAt                            <  2:2365853/93‑1 (MQ=255)

                                                              cGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGAt                            <  1:5385241/93‑1 (MQ=255)

                                                              cGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGAt                            <  1:5031066/93‑1 (MQ=255)

                                                              cGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGAt                            >  2:3270278/1‑93 (MQ=255)

                                                              cGCCTTCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGAt                            <  2:3565403/93‑1 (MQ=255)

                                                                 ctgccGCCACGACGGTTAAATTGACGTGTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGAATGCGTTTTGCGGGCAGACAACGATCGa                         <  2:7008840/90‑1 (MQ=255)

                                                                  ttCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGAc                        >  1:9554855/1‑93 (MQ=255)

                                                                   tCCGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACa                       >  2:5854947/1‑93 (MQ=255)

                                                                    ccGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACaa                      <  2:3133674/93‑1 (MQ=255)

                                                                     cGCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACaaa                     >  1:1963313/1‑93 (MQ=255)

                                                                      gCCACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACaaaa                    <  1:6877843/93‑1 (MQ=255)

                                                                       ccACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAAc                   >  2:294718/1‑93 (MQ=255)

                                                                       ccACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAAc                   >  1:2516421/1‑93 (MQ=255)

                                                                       ccACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAAc                   >  2:6664265/1‑93 (MQ=255)

                                                                        cACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACt                  >  2:3313300/1‑93 (MQ=255)

                                                                        cACGACGGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACt                  <  1:4042927/93‑1 (MQ=255)

                                                                         ccgacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACtt                 <  2:7550259/92‑1 (MQ=255)

                                                                         acgacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACtt                 >  2:5585961/1‑93 (MQ=255)

                                                                          cgacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACttt                >  1:4458958/1‑93 (MQ=255)

                                                                          cgacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACttt                <  2:7726167/93‑1 (MQ=255)

                                                                           gacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACtttt               >  1:1270078/1‑93 (MQ=255)

                                                                           gacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACtttt               <  2:5930504/93‑1 (MQ=255)

                                                                           gacgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACtttt               >  2:1777528/1‑93 (MQ=255)

                                                                             cgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGt             >  2:6109597/1‑93 (MQ=255)

                                                                             cgGTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGt             <  1:9085253/93‑1 (MQ=255)

                                                                              ggTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTc            >  1:5210941/1‑93 (MQ=255)

                                                                              ggTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTc            >  2:6779401/1‑93 (MQ=255)

                                                                              ggTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTc            >  1:8586724/1‑93 (MQ=255)

                                                                              ggTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTc            >  2:37207/1‑93 (MQ=255)

                                                                               gTTAAATTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCa           >  2:8301102/1‑93 (MQ=255)

                                                                                  aaaTTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAAtt        <  1:6674633/93‑1 (MQ=255)

                                                                                  aaaTTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAAtt        >  2:4297201/1‑93 (MQ=255)

                                                                                   aaTTGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAAttt       >  2:131825/1‑93 (MQ=255)

                                                                                      tGAGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAATTTTGa    <  1:7567420/93‑1 (MQ=255)

                                                                                        aGGTTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAATTTTGAtt  <  1:4887673/93‑1 (MQ=255)

ATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCGACGCCTTCCGCCACGACGGTTAAATTGAGGCTTTGCCCAATGCTGGTAATGGCTTCAAGTAAGGCAAGGATGCGTTTTTCGGTCAGACAACGATCGACAAAACTTTTGTCAATTTTGATT  >  NC_000913/1561430‑1561610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`