BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,886	A→G	V299A (GTT→GCT)	b1489 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,886	0	A	G	99.6%	861.7 / ‑5.8	267	V299A (GTT→GCT)	b1489	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (132/134); total (132/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 272 total aligned reads displayed.

GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG  >  NC_000913/1562796‑1562974

gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           <  1:5622097/93‑1 (MQ=255)

gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           >  2:1483560/1‑93 (MQ=255)

gCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           <  2:6451960/93‑1 (MQ=255)

ccTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCa                                                                                           <  2:8796821/92‑1 (MQ=255)

 cTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCAt                                                                                          <  1:1618189/93‑1 (MQ=255)

  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                         >  2:3899653/1‑93 (MQ=255)

  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                         <  1:1572948/93‑1 (MQ=255)

  tGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATg                                                                                         >  1:2312069/1‑93 (MQ=255)

   gACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGc                                                                                        >  2:5797746/1‑93 (MQ=255)

   gACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGc                                                                                        <  1:4565748/93‑1 (MQ=255)

      cAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAt                                                                                     >  1:7311209/1‑93 (MQ=255)

       aGCTATGCGCATTTTGAATTACTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGAtt                                                                                    <  2:9271900/93‑1 (MQ=255)

        gCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTc                                                                                   >  1:7887727/1‑93 (MQ=255)

         cTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCg                                                                                  <  1:8733873/93‑1 (MQ=255)

          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGGTTCGt                                                                                 >  1:2455530/1‑93 (MQ=255)

          tttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGt                                                                                 >  2:5924128/1‑93 (MQ=255)

           ttGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGtt                                                                                <  2:6793768/93‑1 (MQ=255)

            tGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTg                                                                               <  1:9609158/93‑1 (MQ=255)

               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                            <  1:5409074/93‑1 (MQ=255)

               gcATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                            <  1:4152431/93‑1 (MQ=255)

               ccATTTTTAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGt                                                                            <  2:2857058/92‑1 (MQ=255)

                cATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTa                                                                           <  1:3803993/93‑1 (MQ=255)

                 aTTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTAc                                                                          <  2:7164835/93‑1 (MQ=255)

                  ttttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACa                                                                         >  2:5359913/1‑93 (MQ=255)

                  ttttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACa                                                                         >  1:9209882/1‑93 (MQ=255)

                   tttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                        >  1:4320281/1‑93 (MQ=255)

                   tttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                        <  1:3560399/93‑1 (MQ=255)

                   tttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAg                                                                        <  2:6712797/93‑1 (MQ=255)

                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                       <  2:7537219/93‑1 (MQ=255)

                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                       >  1:7589280/1‑93 (MQ=255)

                    ttGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGa                                                                       <  2:3746048/93‑1 (MQ=255)

                      gAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAtt                                                                     <  1:3368829/93‑1 (MQ=255)

                      gAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGAtt                                                                     <  2:5422755/93‑1 (MQ=255)

                      gAATCTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTGGTTGAGTACAGAtt                                                                     <  2:1705206/93‑1 (MQ=255)

                       aaTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTc                                                                    >  1:9037753/1‑93 (MQ=255)

                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                   >  1:6570539/1‑93 (MQ=255)

                        aTTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCg                                                                   >  1:4980671/1‑93 (MQ=255)

                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                  <  1:6887155/93‑1 (MQ=255)

                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                  >  2:8646211/1‑93 (MQ=255)

                         tttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGa                                                                  <  1:252287/93‑1 (MQ=255)

                          ttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAt                                                                 >  1:2689691/1‑93 (MQ=255)

                          ttCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGAt                                                                 <  1:8808148/93‑1 (MQ=255)

                           tCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATg                                                                <  1:4132586/93‑1 (MQ=255)

                             tGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGtt                                                              >  2:6629316/1‑93 (MQ=255)

                               cACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTag                                                            >  2:2991032/1‑92 (MQ=255)

                               cACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTAc                                                            <  1:8359422/93‑1 (MQ=255)

                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                         <  1:1845772/93‑1 (MQ=255)

                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                         <  2:2071588/93‑1 (MQ=255)

                                  cGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGAc                                                         <  1:7059581/93‑1 (MQ=255)

                                   gTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACa                                                        >  2:9601395/1‑93 (MQ=255)

                                    tGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaa                                                       <  2:117581/93‑1 (MQ=255)

                                     gggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACaaa                                                      <  1:805171/93‑1 (MQ=255)

                                      ggAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAAt                                                     >  1:6909306/1‑93 (MQ=255)

                                        aaGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGa                                                   <  1:8762435/93‑1 (MQ=255)

                                         aGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGCAGCATGCGATTCGTTGAGTACAGATTCGATGCTACGACAAATGAt                                                  >  1:4052378/1‑93 (MQ=255)

                                          gATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t                                                <  2:5202359/93‑1 (MQ=255)

                                          gATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑t                                                >  1:5037761/1‑93 (MQ=255)

                                           aTGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAt‑tt                                               >  1:378336/1‑93 (MQ=255)

                                            tGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑c                                             <  1:1014557/93‑1 (MQ=255)

                                            tGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑c                                             >  1:2135513/1‑93 (MQ=255)

                                             gACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc                                            >  2:8403748/1‑93 (MQ=255)

                                              aCGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc‑c                                          >  2:7487621/1‑93 (MQ=255)

                                                gCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc‑ccc                                        <  2:187006/93‑1 (MQ=255)

                                                 cccAGTGTATCGGCATCCCGTTGCGCAGTGCGCACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑cc‑cccc                                       >  2:4885507/1‑92 (MQ=255)

                                                   cAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCaat                                     >  1:4644158/1‑91 (MQ=255)

                                                      tgtATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTc                                   <  1:7148571/93‑1 (MQ=255)

                                                      tgtATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTc                                   >  1:6311351/1‑93 (MQ=255)

                                                        tATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCAt                                 >  2:3058563/1‑93 (MQ=255)

                                                         aTCGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATg                                >  2:6152941/1‑93 (MQ=255)

                                                           cGGCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGaa                              >  1:6455968/1‑93 (MQ=255)

                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            >  2:631295/1‑93 (MQ=255)

                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            <  2:9796664/93‑1 (MQ=255)

                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            >  2:3175329/1‑93 (MQ=255)

                                                             gCATCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAAt                            >  1:5254790/1‑93 (MQ=255)

                                                               aTCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CCACCCATTTCATGAAATgg                          >  2:8113659/1‑93 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  2:7604284/1‑93 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  1:5052059/1‑93 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  1:9655083/1‑93 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  2:1616111/1‑93 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         >  2:1229284/1‑93 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGc                         <  1:9968902/93‑1 (MQ=255)

                                                                tCCCGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCg                        <  2:3477613/93‑1 (MQ=255)

                                                                 cccGTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCg                        <  1:83146/93‑1 (MQ=255)

                                                                   cGTGGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGgc                     <  2:1305785/93‑1 (MQ=255)

                                                                    gTTGCGCAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGgc                     <  1:275849/93‑1 (MQ=255)

                                                                         gcAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGctgctg                <  1:7781197/93‑1 (MQ=255)

                                                                          cAGTGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGATGTC‑CC‑CCCATTTCATGAAATGGCGGGCtgatgc               >  1:588989/1‑93 (MQ=255)

                                                                             tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAACTGGCGGGCTGCTGccca            >  2:2606729/1‑93 (MQ=255)

                                                                             tGCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGcaca            >  1:9339607/1‑93 (MQ=255)

                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  2:9098153/93‑1 (MQ=255)

                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  1:1301859/93‑1 (MQ=255)

                                                                              gCGAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACAt           <  1:8627655/93‑1 (MQ=255)

                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATTTTACGACAAATGAT‑TT‑CCTCCCACTTCATGAAATGGCGGTCTTCTGCACCTgg         >  2:7073991/1‑93 (MQ=255)

                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATgg         >  2:2244908/1‑93 (MQ=255)

                                                                                gAACAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATgg         <  2:4636682/93‑1 (MQ=255)

                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggc        >  2:2496246/1‑93 (MQ=255)

                                                                                 aaCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggc        <  2:4245986/93‑1 (MQ=255)

                                                                                  aCAGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggcg       >  1:2917401/1‑93 (MQ=255)

                                                                                    aGCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TTCCC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATggcggc     <  2:4053112/93‑1 (MQ=255)

                                                                                     gCGAAGCATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCga   <  2:5957030/93‑1 (MQ=255)

GCTGACCAGCTTTGCGCATTTTGAATTTCTGCACCGTGGGAAGATGACGCCCAGTGTATCGGCATCCCGTTGCGCAGTGCGAACAGCGAAACATGCGATTCGTTGAGTACAGATTCGATGTTACGACAAATGAT‑TT‑CC‑CCCATTTCATGAAATGGCGGGCTGCTGCACATGGCGGCGAG  >  NC_000913/1562796‑1562974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`