BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,386	C→T	R80H (CGC→CAC)	yadS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,386	0	C	T	99.7%	1116.8 / NA	318	R80H (CGC→CAC)	yadS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (167/150); minor base G (0/1); total (167/151)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 348 total aligned reads displayed.

AAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGCGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGC  >  NC_000913/177296‑177475

aaGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGgtgc                                                                                         >  1:5961832/1‑93 (MQ=255)

aaGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGgtgc                                                                                         >  1:7231777/1‑93 (MQ=255)

aaGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGgtgc                                                                                         <  1:2947951/93‑1 (MQ=255)

aaGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGgtgc                                                                                         <  2:5133648/93‑1 (MQ=255)

 aGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCa                                                                                        <  1:10043020/93‑1 (MQ=255)

   gCTTTATTCACGCCAATGCCGACAAACCCCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCAcc                                                                                      <  2:2783633/93‑1 (MQ=255)

   gCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCAcc                                                                                      >  1:692183/1‑93 (MQ=255)

   gCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCAcc                                                                                      <  2:3161818/93‑1 (MQ=255)

     tttATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAg                                                                                    >  2:4338858/1‑93 (MQ=255)

       tATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCa                                                                                  <  1:9590038/93‑1 (MQ=255)

       tATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCa                                                                                  >  2:4848310/1‑93 (MQ=255)

       tATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCa                                                                                  <  1:6112832/93‑1 (MQ=255)

        aTTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGATGGTTAACCAGCAc                                                                                 >  2:2991970/1‑93 (MQ=255)

         ttCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACg                                                                                >  2:8179200/1‑93 (MQ=255)

          tCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGa                                                                               <  1:8230309/93‑1 (MQ=255)

           cACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGAt                                                                              <  1:9268021/93‑1 (MQ=255)

            aCGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATg                                                                             <  2:9731602/93‑1 (MQ=255)

            aCGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATg                                                                             >  2:7935533/1‑93 (MQ=255)

            aCGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATg                                                                             >  1:1508330/1‑93 (MQ=255)

            aCGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGGCTTGGCTGGTGCACCAGCACGATg                                                                             >  2:7415338/1‑93 (MQ=255)

              gCCAATGCCGACGAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGt                                                                           <  1:3312234/93‑1 (MQ=255)

              gCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCATCATCCATTTTGGGAAGCGGCTTGGCGGGTGCACCAGCACGCTGGt                                                                           >  2:7361479/1‑93 (MQ=255)

               ccAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTc                                                                          <  2:7529095/93‑1 (MQ=255)

               ccAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTc                                                                          <  1:7425811/93‑1 (MQ=255)

                cAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCa                                                                         >  1:6700447/1‑93 (MQ=255)

                 aaTGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTTAg                                                                        >  1:1337469/1‑93 (MQ=255)

                  aTGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGc                                                                       <  1:1230966/93‑1 (MQ=255)

                  aTGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGc                                                                       >  1:5672335/1‑93 (MQ=255)

                  aTGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGc                                                                       >  2:892919/1‑93 (MQ=255)

                   tGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCGCGATGGTCAGCa                                                                      <  1:9999656/93‑1 (MQ=255)

                   tGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCa                                                                      >  1:9038356/1‑93 (MQ=255)

                   tGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCa                                                                      <  1:195468/93‑1 (MQ=255)

                   tGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCa                                                                      <  1:5225828/93‑1 (MQ=255)

                      cGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACCATGGTCAGCATGc                                                                   >  2:1759607/1‑93 (MQ=255)

                       gACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACGTCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCt                                                                  >  2:2080495/1‑93 (MQ=255)

                       gACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCt                                                                  >  2:4798518/1‑93 (MQ=255)

                         cAAACGCCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTgg                                                                <  1:8076651/93‑1 (MQ=255)

                         cAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTgg                                                                <  1:7094571/93‑1 (MQ=255)

                         cAAACACCGCCAGACCAACGCCGTCCAGCACCGCCAACATCCATTTTGGTAAGCGTCTTGGCTGCTACACCAGAACGATGGTCAGCATGCTgg                                                                <  1:5473507/93‑1 (MQ=255)

                          aagcacCGCCAGACCAACGACGTTCAGCACCGGCAACATCCATTTTGGTAAGGGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGt                                                               <  2:6919869/90‑1 (MQ=255)

                          aaaCACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGt                                                               <  2:3906371/93‑1 (MQ=255)

                             cgccGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGAc                                                            >  1:2463457/3‑93 (MQ=255)

                             cacCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATCGTCAGCATGCTGGTGAc                                                            >  2:6714578/1‑93 (MQ=255)

                               ccGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCa                                                          <  2:1435867/93‑1 (MQ=255)

                               ccGACAGACCAGCGCCCGCGAGCACCGGACACATCGATTGTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCa                                                          <  1:8501478/93‑1 (MQ=255)

                                cGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCAt                                                         >  1:2126288/1‑93 (MQ=255)

                                   cAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGc                                                      <  1:3552787/93‑1 (MQ=255)

                                   cAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGc                                                      >  1:5390105/1‑93 (MQ=255)

                                     gACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCaa                                                    >  2:3769357/1‑93 (MQ=255)

                                     gACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCaa                                                    <  2:2211547/93‑1 (MQ=255)

                                      aCCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAAc                                                   <  1:2789912/93‑1 (MQ=255)

                                         aaCGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGAc                                                >  2:1539/1‑93 (MQ=255)

                                         aaCGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGAc                                                >  2:910463/1‑93 (MQ=255)

                                         aaCGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGAc                                                >  2:5301190/1‑93 (MQ=255)

                                               gTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATc                                          <  2:7797425/93‑1 (MQ=255)

                                                tCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCg                                         <  1:4162513/93‑1 (MQ=255)

                                                  cAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGt                                       >  1:6386677/1‑93 (MQ=255)

                                                  cAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGt                                       <  2:3284392/93‑1 (MQ=255)

                                                   aGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTg                                      <  2:1687921/93‑1 (MQ=255)

                                                    gCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTgg                                     <  2:4163753/93‑1 (MQ=255)

                                                    gCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTgg                                     <  1:5280803/93‑1 (MQ=255)

                                                       ccGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGAt                                  >  1:9130528/1‑93 (MQ=255)

                                                        cGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATc                                 >  1:9498794/1‑93 (MQ=255)

                                                          gCAACATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCtt                               <  2:8725941/93‑1 (MQ=255)

                                                           gaaCATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACTATTGCAACGACCAGATCGGTGGGATCttt                              <  1:3211417/92‑1 (MQ=255)

                                                            aaCATCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTc                             >  2:2458797/1‑93 (MQ=255)

                                                              cATCCATTTTGGTAAGCGTCTTGGCTGGGGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCAc                           <  1:2361818/93‑1 (MQ=255)

                                                                tCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCAccc                         >  2:5493481/1‑93 (MQ=255)

                                                                tCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCAccc                         >  2:5715163/1‑93 (MQ=255)

                                                                tCCATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCAccc                         >  2:9925089/1‑93 (MQ=255)

                                                                 ccATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCa                        >  1:2877771/1‑93 (MQ=255)

                                                                  cATTTTGGTAAGCGTCTTGTCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCaa                       <  2:4047220/93‑1 (MQ=255)

                                                                  cATTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCaa                       <  1:3789023/93‑1 (MQ=255)

                                                                   aTTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCaaa                      >  2:449275/1‑93 (MQ=255)

                                                                   aTTTTGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCaaa                      >  1:7577086/1‑93 (MQ=255)

                                                                    ttttGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCaaaa                     <  2:945636/93‑1 (MQ=255)

                                                                    ttttGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCaaaa                     >  1:7962599/1‑93 (MQ=255)

                                                                     tttGGGAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAAt                    <  1:1598033/93‑1 (MQ=255)

                                                                       tGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATAc                  >  2:3846690/1‑93 (MQ=255)

                                                                       tGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATAc                  <  2:4972623/93‑1 (MQ=255)

                                                                       tGGTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATAc                  <  1:4554583/93‑1 (MQ=255)

                                                                        ggTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATAcc                 <  2:3645860/93‑1 (MQ=255)

                                                                        ggTAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATAcc                 <  1:4494043/93‑1 (MQ=255)

                                                                          tAAGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCgg               >  1:8405461/1‑93 (MQ=255)

                                                                           aaGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCggg              <  1:8653608/93‑1 (MQ=255)

                                                                            aGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGc             >  2:4733088/1‑93 (MQ=255)

                                                                            aGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGc             <  2:2604586/93‑1 (MQ=255)

                                                                            aGCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGc             <  2:638468/93‑1 (MQ=255)

                                                                            aGCGTCTTGGCGGGTGCACCAGCACGATGGACAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATGCCGGGc             <  1:2632207/93‑1 (MQ=255)

                                                                             gCGTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGcc            >  1:9921597/1‑93 (MQ=255)

                                                                               gTCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGt          <  1:3050941/93‑1 (MQ=255)

                                                                                tCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAACTACCGGGCCGTg         >  1:8799099/1‑93 (MQ=255)

                                                                                tCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTg         >  1:590059/1‑93 (MQ=255)

                                                                                tCTTGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTg         >  2:9331426/1‑93 (MQ=255)

                                                                                  ttGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGAt       <  2:3064698/93‑1 (MQ=255)

                                                                                  ttGGCTGGTGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGAt       >  2:6566280/1‑93 (MQ=255)

                                                                                    ggctggtGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATcc     <  2:1319649/93‑1 (MQ=255)

                                                                                     gctggtGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCa    >  2:9525579/1‑93 (MQ=255)

                                                                                      ctggtGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAg   <  2:5915673/93‑1 (MQ=255)

                                                                                       tggtGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAgc  <  2:8459066/93‑1 (MQ=255)

AAGGCTTTATTCACGCCAATGCCGACAAACACCGCCAGACCAACGGCGTCCAGCACCGGCAACATCCATTTTGGTAAGCGTCTTGGCTGGCGCACCAGCACGATGGTCAGCATGCTGGTGACCATTGCAACGACCAGATCGGTGGGATCTTTCACCCAAAATACCGGGCCGTGATCCAGC  >  NC_000913/177296‑177475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`