BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,627,932	A→G	L336L (TTG→CTG)	ydfI ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,627,932	0	A	G	90.4%	653.9 / 46.2	251	L336L (TTG→CTG)	ydfI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (11/13); new base G (118/109); total (129/122)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 256 total aligned reads displayed.

AATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAA  >  NC_000913/1627840‑1628024

  tCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCg                                                                                            >  1:2399626/1‑93 (MQ=255)

  tCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCg                                                                                            >  2:2474026/1‑93 (MQ=255)

   cTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGt                                                                                           <  2:2312153/93‑1 (MQ=255)

   cTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGt                                                                                           >  2:1276412/1‑93 (MQ=255)

    tGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTc                                                                                          >  2:1613477/1‑93 (MQ=255)

      ccAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCgg                                                                                        <  1:838574/93‑1 (MQ=255)

        aGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGgcg                                                                                      >  2:3003977/1‑93 (MQ=255)

        aGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTAAGCGTCGgcg                                                                                      >  1:8820360/1‑93 (MQ=255)

         ggTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGgcgc                                                                                     >  1:322257/1‑93 (MQ=255)

          gTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCt                                                                                    <  1:2702490/93‑1 (MQ=255)

          gTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCt                                                                                    >  1:360612/1‑93 (MQ=255)

          gTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCt                                                                                    >  2:6137737/1‑93 (MQ=255)

           ttCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCtt                                                                                   <  2:9545044/93‑1 (MQ=255)

            tCGATTACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTg                                                                                  <  2:5983097/93‑1 (MQ=255)

                tGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTcc                                                                              >  2:8391357/1‑93 (MQ=255)

                 gACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCt                                                                             >  1:6041274/1‑93 (MQ=255)

                     tAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCaa                                                                         >  2:4093968/1‑93 (MQ=255)

                      aaCGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAAc                                                                        <  2:2127603/93‑1 (MQ=255)

                      aaCGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAAc                                                                        <  1:7068052/93‑1 (MQ=255)

                        cGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACAt                                                                      <  2:864583/93‑1 (MQ=255)

                        cGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACAt                                                                      <  1:4401200/93‑1 (MQ=255)

                         gCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATc                                                                     >  2:8140588/1‑93 (MQ=255)

                           cGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCaa                                                                   <  1:10004991/93‑1 (MQ=255)

                           cGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCaa                                                                   <  1:2479308/93‑1 (MQ=255)

                           cGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCaa                                                                   <  2:8221702/93‑1 (MQ=255)

                            gggTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAg                                                                  >  2:7172623/1‑93 (MQ=255)

                             ggTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGc                                                                 <  1:919590/93‑1 (MQ=255)

                             ggTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGc                                                                 >  1:3030599/1‑93 (MQ=255)

                             ggTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGc                                                                 >  1:5147061/1‑93 (MQ=255)

                              gTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGcc                                                                >  1:9331547/1‑93 (MQ=255)

                              gTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGcc                                                                <  2:3534778/93‑1 (MQ=255)

                                 gCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATa                                                             >  1:2272066/1‑93 (MQ=255)

                                  cTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAc                                                            >  1:6385535/1‑93 (MQ=255)

                                   tataGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcg                                                           >  2:8547921/1‑93 (MQ=255)

                                    ataGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgc                                                          >  2:5758478/1‑93 (MQ=255)

                                    ataGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgc                                                          >  1:6695718/1‑93 (MQ=255)

                                    ataGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgc                                                          >  2:4746972/1‑93 (MQ=255)

                                     taGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgcc                                                         >  2:872096/1‑93 (MQ=255)

                                     taGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgcc                                                         <  2:9015856/93‑1 (MQ=255)

                                     taGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgcc                                                         >  1:5377286/1‑93 (MQ=255)

                                     taGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgcc                                                         >  2:6165519/1‑93 (MQ=255)

                                     taGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgcc                                                         <  2:31475/93‑1 (MQ=255)

                                     taGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATAcgcc                                                         <  2:6392226/93‑1 (MQ=255)

                                        cGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCa                                                      <  2:4234906/93‑1 (MQ=255)

                                         gTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCTACATCAAGCCATACGCCGCAt                                                     >  1:3841346/1‑93 (MQ=255)

                                         gTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCAt                                                     >  2:1425931/1‑93 (MQ=255)

                                          tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATa                                                    >  2:5646094/1‑93 (MQ=255)

                                           ggaaTTAATCTGTTATCGTAATTTTGCAACACAACGCCCTTCACTTTCAACGTCGGCGCTTGTTCCTGCAACACCAAGCCATACGCCGCATaa                                                   <  2:3480969/91‑1 (MQ=255)

                                           gCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATaa                                                   <  2:9849093/93‑1 (MQ=255)

                                           gCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATaa                                                   <  1:4227207/93‑1 (MQ=255)

                                           gCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATaa                                                   <  2:3998510/93‑1 (MQ=255)

                                            cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAAc                                                  >  1:10021323/1‑93 (MQ=255)

                                              aTTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGa                                                >  1:2057355/1‑93 (MQ=255)

                                              aTTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGa                                                <  1:4104783/93‑1 (MQ=255)

                                              aTTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGa                                                >  1:8718962/1‑93 (MQ=255)

                                               ttAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGAt                                               <  2:106181/93‑1 (MQ=255)

                                                tAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATa                                              >  1:8999209/1‑93 (MQ=255)

                                                tAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATa                                              <  1:9882312/93‑1 (MQ=255)

                                                 aaTCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATaa                                             <  2:7681678/93‑1 (MQ=255)

                                                  aTCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAAt                                            <  2:8476988/93‑1 (MQ=255)

                                                   tCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATg                                           >  2:4353867/1‑93 (MQ=255)

                                                     ggTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGtt                                         >  1:5929296/1‑93 (MQ=255)

                                                     ggTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGtt                                         <  1:132583/93‑1 (MQ=255)

                                                     ggTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGtt                                         <  2:3846070/93‑1 (MQ=255)

                                                      gTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                        <  1:1231447/93‑1 (MQ=255)

                                                      gTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTc                                        >  2:6777733/1‑93 (MQ=255)

                                                       ttAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCa                                       <  1:1464252/93‑1 (MQ=255)

                                                       ttAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCa                                       <  1:6872145/93‑1 (MQ=255)

                                                        tAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCAt                                      <  2:9685849/93‑1 (MQ=255)

                                                         aGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATc                                     <  1:6581323/93‑1 (MQ=255)

                                                          gCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCt                                    >  2:5046472/1‑93 (MQ=255)

                                                          gCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCt                                    >  2:2940211/1‑93 (MQ=255)

                                                          gCGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCt                                    <  1:2162777/93‑1 (MQ=255)

                                                           cGTAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCtt                                   <  1:7323570/93‑1 (MQ=255)

                                                             tAATCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTcc                                 >  1:3135531/1‑93 (MQ=255)

                                                              aaTCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCa                                <  1:9388004/93‑1 (MQ=255)

                                                               aTCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                               <  2:9417877/93‑1 (MQ=255)

                                                               aTCTTGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                               >  1:8909206/1‑93 (MQ=255)

                                                                  ttGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACa                            >  2:4551977/1‑93 (MQ=255)

                                                                  ttGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACa                            <  2:2907600/93‑1 (MQ=255)

                                                                  ttGCAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACa                            >  2:6721917/1‑93 (MQ=255)

                                                                     cAAATCAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTc                         >  1:4493214/1‑93 (MQ=255)

                                                                        aTCAACCCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTAtt                      >  1:7682563/1‑93 (MQ=255)

                                                                          cAACGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTaa                    <  2:2593437/93‑1 (MQ=255)

                                                                           aaCGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAAt                   <  2:1818042/93‑1 (MQ=255)

                                                                            aCGCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAAtg                  <  1:7420895/93‑1 (MQ=255)

                                                                              gCCCTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAAtgtg                <  2:9697772/93‑1 (MQ=255)

                                                                               cccTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAATGTGc               <  1:5070192/93‑1 (MQ=255)

                                                                                ccTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGCTAATGTTCATCTTCCATACAGTTATTAATGTGCt              >  2:7368263/1‑93 (MQ=255)

                                                                                ccTGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCt              <  1:4934042/93‑1 (MQ=255)

                                                                                  tGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAATGTGCTGa            >  2:3711126/1‑93 (MQ=255)

                                                                                  tGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGa            >  2:2051878/1‑93 (MQ=255)

                                                                                  tGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGa            >  2:5375004/1‑93 (MQ=255)

                                                                                  tGCACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGa            >  2:5167431/1‑93 (MQ=255)

                                                                                    cACTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGata          <  1:1659424/93‑1 (MQ=255)

                                                                                     aCTTTCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAATGTGCTGatat         >  1:45973/1‑93 (MQ=255)

                                                                                       tttCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAATGTGCTGATATcc       >  1:8068996/1‑93 (MQ=255)

                                                                                        ttCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTTATTAATGTGCTGATATCCt      <  2:2509112/93‑1 (MQ=255)

                                                                                        ttCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCt      >  2:6835015/1‑93 (MQ=255)

                                                                                         tCAGCGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTg     <  2:9276623/93‑1 (MQ=255)

AATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAA  >  NC_000913/1627840‑1628024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`