BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,245	T→C	L91L (TTA→TTG)	ynfC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,245	0	T	C	99.4%	1022.8 / ‑5.1	313	L91L (TTA→TTG)	ynfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (173/138); total (174/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

Only 100 of 319 total aligned reads displayed.

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGT  >  NC_000913/1655155‑1655334

 aaaCGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAAt                                                                                        >  2:6242127/1‑93 (MQ=255)

   aCGTACTCTCTTCTCCAGCGTCTCGGCATCATGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTc                                                                                      <  2:8521743/93‑1 (MQ=255)

    cGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCg                                                                                     <  1:6005333/93‑1 (MQ=255)

      tACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAg                                                                                   <  1:1079785/93‑1 (MQ=255)

       tctctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                  <  2:2457373/92‑1 (MQ=255)

       aCTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGt                                                                                  >  1:1169075/1‑93 (MQ=255)

        ctctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTg                                                                                 >  2:4712936/1‑93 (MQ=255)

        ctctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTACCAGATCCAGCAATTCGAGTg                                                                                 <  1:5909031/93‑1 (MQ=255)

         tctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGa                                                                                <  2:5526111/93‑1 (MQ=255)

         tctctTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGa                                                                                <  2:4294/93‑1 (MQ=255)

           tcttctCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAAt                                                                              <  2:1496964/93‑1 (MQ=255)

            ctgctcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATc                                                                             <  2:2028332/90‑1 (MQ=255)

              tctcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaa                                                                           >  1:8304643/1‑93 (MQ=255)

              tctcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaa                                                                           >  1:2730086/1‑93 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                         <  1:6441996/93‑1 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                         >  2:3959342/1‑93 (MQ=255)

                tcCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCaaaa                                                                         >  1:7028128/1‑93 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                        <  2:2296588/93‑1 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                        <  1:2016653/93‑1 (MQ=255)

                 ccAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAAc                                                                        <  1:7807175/93‑1 (MQ=255)

                  cAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACa                                                                       <  1:5377723/93‑1 (MQ=255)

                  cAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACa                                                                       >  1:1312729/1‑93 (MQ=255)

                   aGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACac                                                                      >  1:5076323/1‑92 (MQ=255)

                     cGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGcc                                                                    <  2:5912149/93‑1 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   >  2:4098592/1‑93 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   >  1:4965343/1‑93 (MQ=255)

                      gTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCct                                                                   <  1:1483072/93‑1 (MQ=255)

                       tctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCctt                                                                  >  2:3535864/1‑93 (MQ=255)

                       tctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCctt                                                                  >  2:6984695/1‑93 (MQ=255)

                        ctcGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCcttc                                                                 <  2:6352108/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              <  1:6976053/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              <  1:7316595/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              <  1:586179/93‑1 (MQ=255)

                           ggCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCttcttc                                                              <  2:3235764/93‑1 (MQ=255)

                               tCACGTTAATAATTGGCGTCCAGTACCAGTGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAATCAGCCTTCTTCCGAc                                                          >  1:8957042/1‑93 (MQ=255)

                               tCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGAc                                                          <  2:9786198/93‑1 (MQ=255)

                               tCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGAc                                                          >  1:1334242/1‑93 (MQ=255)

                                  cGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACaaa                                                       <  1:6732490/93‑1 (MQ=255)

                                    gTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGt                                                     >  2:7858791/1‑93 (MQ=255)

                                     taataaTTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTc                                                    >  2:598985/1‑93 (MQ=255)

                                        taatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCa                                                 >  2:9577255/1‑93 (MQ=255)

                                        taatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCa                                                 >  2:7213545/1‑93 (MQ=255)

                                        taatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCa                                                 >  1:802964/1‑93 (MQ=255)

                                        taatTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCa                                                 <  1:8415115/93‑1 (MQ=255)

                                           tttgCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaa                                              <  1:1976192/90‑1 (MQ=255)

                                           ttGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaa                                              <  1:5153062/93‑1 (MQ=255)

                                            tGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGaaa                                             <  1:4368721/93‑1 (MQ=255)

                                             ggCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAac                                            >  1:8749819/1‑93 (MQ=255)

                                                gTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGACACACg                                         >  1:3852881/1‑93 (MQ=255)

                                                gTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACg                                         >  2:375339/1‑93 (MQ=255)

                                                gTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACg                                         >  1:758063/1‑93 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                        <  1:5702846/93‑1 (MQ=255)

                                                 tCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGt                                        <  1:8563355/93‑1 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                       >  2:4909344/1‑93 (MQ=255)

                                                  ccAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGtt                                       <  1:1703764/93‑1 (MQ=255)

                                                    aGTACCCGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTc                                     <  1:7645069/93‑1 (MQ=255)

                                                     gTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGAGAATCACAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCg                                    >  2:5193989/1‑93 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                 <  1:6801578/93‑1 (MQ=255)

                                                        ccAGAGCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCa                                 <  1:9823576/93‑1 (MQ=255)

                                                             gCGACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCa                            <  2:7991204/93‑1 (MQ=255)

                                                               gACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCAcc                          >  2:7492723/1‑93 (MQ=255)

                                                               gACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCAcc                          <  2:7398103/93‑1 (MQ=255)

                                                               gACCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTGCTCCCGACAAAGTCCCAGAAACCCGTTTCGTCACTTcccc                          <  2:496260/93‑1 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                         >  2:5841672/1‑93 (MQ=255)

                                                                aCCACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCt                         <  2:565855/93‑1 (MQ=255)

                                                                 ccACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCtt                        <  2:8927579/93‑1 (MQ=255)

                                                                  cACGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTg                       >  1:4792037/1‑93 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      >  1:6025487/1‑93 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      >  2:2969856/1‑93 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      >  2:173179/1‑93 (MQ=255)

                                                                   aCGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGc                      <  1:7388003/93‑1 (MQ=255)

                                                                    cGGTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAAACAGTTTCGCCACTTCCCCttcgt                     >  1:2903619/1‑90 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                    >  2:8827295/1‑93 (MQ=255)

                                                                     ggTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTc                    >  2:1385221/1‑93 (MQ=255)

                                                                      gTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                   <  1:7952618/93‑1 (MQ=255)

                                                                      gTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                   >  1:2429572/1‑93 (MQ=255)

                                                                      gTATTATTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCa                   >  2:6730471/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                  >  2:3300439/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                  >  2:1620944/1‑93 (MQ=255)

                                                                       tattatTTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCAt                  >  2:6112556/1‑93 (MQ=255)

                                                                        attattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATc                 <  1:3909262/93‑1 (MQ=255)

                                                                         ttattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATcc                >  1:3494467/1‑93 (MQ=255)

                                                                         ttattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATcc                >  1:1918732/1‑93 (MQ=255)

                                                                          tattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCa               <  1:9466181/93‑1 (MQ=255)

                                                                          tattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCa               >  2:399407/1‑93 (MQ=255)

                                                                          tattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCa               >  2:2677714/1‑93 (MQ=255)

                                                                          tattTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCa               <  1:4642398/93‑1 (MQ=255)

                                                                           attTTCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAt              >  2:8589221/1‑93 (MQ=255)

                                                                            ttttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCAtt             >  1:9167590/1‑93 (MQ=255)

                                                                             tttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTa            >  1:8247996/1‑93 (MQ=255)

                                                                             tttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTa            <  1:5239340/93‑1 (MQ=255)

                                                                              ttCCAGATCCAGCAATTCGAGTGAATCCAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGCTTCGTCACTTCACCTTGCTCATCCATTaa           >  1:3040419/1‑93 (MQ=255)

                                                                              ttCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTaa           <  2:254484/93‑1 (MQ=255)

                                                                               tCCAGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAAt          <  1:10024103/93‑1 (MQ=255)

                                                                                  aGATCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTc       <  2:3439833/93‑1 (MQ=255)

                                                                                    aTCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTg     <  2:8834373/93‑1 (MQ=255)

                                                                                     tCCAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa    >  2:8180393/1‑93 (MQ=255)

                                                                                     accAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGa    <  2:12154/92‑1 (MQ=255)

                                                                                      ccAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAg   >  1:3298568/1‑93 (MQ=255)

                                                                                       cAGCAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGt  >  1:4063409/1‑93 (MQ=255)

TAAACGTACTCTCTTCTCCAGCGTCTCGGCATCACGGTAATAATTGGCGTCCAGTACCAGAGCGACCACGGTATTATTTTCCAGATCCAGTAATTCGAGTGAATCAAAACAGCCTTCTTCCGACAAAGTCCCAGAAACACGTTTCGTCACTTCACCTTGCTCATCCATTAATGTCTGAGT  >  NC_000913/1655155‑1655334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`