Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,601 T→C A27A (GCT→GCC)  b1591 → putative oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,6010TC99.5% 597.4 / ‑4.8 187A27A (GCT→GCC) b1591putative oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (80/106);  total (81/106)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 194 total aligned reads displayed.

ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690
                                                                                          |                                                                                         
acaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGcccc                                                                                         <  2:1850688/93‑1 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  1:1313573/93‑1 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        >  2:2735609/1‑93 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        >  1:7894644/1‑93 (MQ=255)
 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  1:3412040/93‑1 (MQ=255)
     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    <  1:5203233/93‑1 (MQ=255)
       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  >  2:7681213/1‑93 (MQ=255)
        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 <  1:9129002/93‑1 (MQ=255)
        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 >  1:8885845/1‑93 (MQ=255)
        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 <  1:5509270/93‑1 (MQ=255)
         gATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGc                                                                                >  2:9588757/1‑93 (MQ=255)
          aTGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCg                                                                               <  1:5806557/93‑1 (MQ=255)
          aTGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCg                                                                               >  2:5367121/1‑93 (MQ=255)
           tGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCgg                                                                              >  1:6126780/1‑93 (MQ=255)
            gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGa                                                                             <  1:6648122/93‑1 (MQ=255)
            gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGa                                                                             >  1:9001692/1‑93 (MQ=255)
             ttAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGaa                                                                            <  2:4727351/93‑1 (MQ=255)
               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          <  1:3232608/93‑1 (MQ=255)
               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          <  2:3853074/93‑1 (MQ=255)
                ggAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGcc                                                                         <  2:6807819/93‑1 (MQ=255)
                 gAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCg                                                                        >  1:8748728/1‑93 (MQ=255)
                 gAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCg                                                                        >  2:8563006/1‑93 (MQ=255)
                  aaTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGc                                                                       <  1:3071807/93‑1 (MQ=255)
                   aTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCa                                                                      >  2:7843188/1‑93 (MQ=255)
                    tGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAc                                                                     <  1:4261462/93‑1 (MQ=255)
                     gACCCATTTTTCACAGCACGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAcc                                                                    >  2:5402769/1‑93 (MQ=255)
                       cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  <  1:7259994/93‑1 (MQ=255)
                        ccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctct                                                                 <  1:4050864/93‑1 (MQ=255)
                          aTTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTgg                                                               >  2:8094364/1‑93 (MQ=255)
                          aTTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTgg                                                               >  1:3309283/1‑93 (MQ=255)
                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              >  1:3361672/1‑93 (MQ=255)
                             tttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTg                                                            <  2:2593639/93‑1 (MQ=255)
                             tttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTg                                                            <  2:2123260/93‑1 (MQ=255)
                              ttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGc                                                           <  2:7529221/93‑1 (MQ=255)
                               tCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCg                                                          >  2:3292568/1‑93 (MQ=255)
                               tCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCg                                                          >  2:5998266/1‑93 (MQ=255)
                                cacaGCAAGATAATGTTTCGGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTTCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGTTTGCgg                                                         <  1:2382495/93‑1 (MQ=255)
                                 acaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGt                                                        <  1:8518147/93‑1 (MQ=255)
                                 acaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGGTGCGGt                                                        >  2:3769504/1‑93 (MQ=255)
                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTCCTCAc                                                  >  1:568670/1‑93 (MQ=255)
                                        gATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAcc                                                 >  1:9143443/1‑93 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGGACTCACCa                                                >  2:3448296/1‑93 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:3288415/93‑1 (MQ=255)
                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:780705/93‑1 (MQ=255)
                                          tAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAg                                               <  2:8506243/93‑1 (MQ=255)
                                            aTTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTg                                             <  1:8383546/93‑1 (MQ=255)
                                              ttttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAc                                           >  1:1874355/1‑93 (MQ=255)
                                              ttttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAc                                           >  1:3491010/1‑93 (MQ=255)
                                               tttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACg                                          <  2:3931730/93‑1 (MQ=255)
                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         >  1:4911387/1‑93 (MQ=255)
                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         >  1:7533834/1‑93 (MQ=255)
                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         <  1:6864378/93‑1 (MQ=255)
                                                 tCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTCCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  1:3592783/93‑1 (MQ=255)
                                                 tCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  1:1157023/93‑1 (MQ=255)
                                                 tCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  1:7093726/93‑1 (MQ=255)
                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGGAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      >  2:9055966/1‑93 (MQ=255)
                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      >  2:8702982/1‑93 (MQ=255)
                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      <  2:4151265/93‑1 (MQ=255)
                                                   tGTCGCGGCGCGCGTGGTGGGCGCGCTGTTTTATTACGCCCCAGATAGTGCGGAAGCCGCACCTCTGGGTGCGGTACTCATCAGTGTCGGCTg                                      >  2:8962388/1‑93 (MQ=255)
                                                    gTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAGGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTgg                                     <  1:6443634/93‑1 (MQ=255)
                                                    gTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTgg                                     >  2:4553341/1‑93 (MQ=255)
                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTggg                                    <  1:1610062/93‑1 (MQ=255)
                                                      cgcgGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGa                                   <  1:2325896/93‑1 (MQ=255)
                                                       gcggcgCGCGTGTTGGGCGCCCTGTTTTATTACGCCCCAGAGAGTGCGGAAACCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGaa                                  <  1:5919883/93‑1 (MQ=255)
                                                          gcgcgcGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGAGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACt                               >  1:2077254/1‑93 (MQ=255)
                                                           cgcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTc                              <  1:3157531/93‑1 (MQ=255)
                                                           cgcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTc                              >  2:7370097/1‑93 (MQ=255)
                                                           cgcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTc                              <  2:9272417/93‑1 (MQ=255)
                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            >  1:1997724/1‑93 (MQ=255)
                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            <  1:8183357/93‑1 (MQ=255)
                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            <  1:6948381/93‑1 (MQ=255)
                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:6391116/93‑1 (MQ=255)
                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:9347607/93‑1 (MQ=255)
                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:6778499/93‑1 (MQ=255)
                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  2:9447180/93‑1 (MQ=255)
                                                                 tgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGACACTCAGTTGc                        >  2:9301165/1‑93 (MQ=255)
                                                                  gtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGcc                       <  1:5025163/93‑1 (MQ=255)
                                                                  gtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGcc                       >  1:6979606/1‑93 (MQ=255)
                                                                   ttGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCct                      >  2:8664004/1‑93 (MQ=255)
                                                                   ttGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCct                      >  1:3436196/1‑93 (MQ=255)
                                                                      ggCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTa                   <  1:2265188/93‑1 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCCGTCGCCTCTACCa                >  2:138133/1‑93 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                >  2:9882174/1‑93 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                <  1:4125826/93‑1 (MQ=255)
                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                <  2:1660516/93‑1 (MQ=255)
                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               <  2:3354477/93‑1 (MQ=255)
                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               <  2:2203276/93‑1 (MQ=255)
                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               >  2:1599621/1‑93 (MQ=255)
                                                                           gcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGa              <  2:2992899/93‑1 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          <  2:8885844/93‑1 (MQ=255)
                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          <  2:5185013/93‑1 (MQ=255)
                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  1:9603298/93‑1 (MQ=255)
                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  1:4365240/93‑1 (MQ=255)
                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  2:9848520/93‑1 (MQ=255)
                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  2:624545/93‑1 (MQ=255)
                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      <  2:1229691/93‑1 (MQ=255)
                                                                                     taCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTa    <  1:2171217/93‑1 (MQ=255)
                                                                                     taCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTa    >  1:531731/1‑93 (MQ=255)
                                                                                      aCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAg   <  1:2570291/93‑1 (MQ=255)
                                                                                       cGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc  <  2:6437188/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑