BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,684,672	A→G	intergenic (‑60/+83)	fumC ← / ← fumA	fumarase C/fumarase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,672	0	A	G	100.0%	983.1 / NA	299	intergenic (‑60/+83)	fumC/fumA	fumarase C/fumarase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (139/160); total (139/160)

Only 100 of 304 total aligned reads displayed.

TCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTC  >  NC_000913/1684582‑1684761

tCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGc                                                                                          >  1:3376240/1‑93 (MQ=255)

 cGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCt                                                                                         <  2:6609554/93‑1 (MQ=255)

  gAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTc                                                                                        <  2:9885649/93‑1 (MQ=255)

     tCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑Ct                                                                                    >  2:4778320/1‑93 (MQ=255)

      cTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑Ctt                                                                                   <  2:3594336/93‑1 (MQ=255)

      cTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑Ctt                                                                                   <  1:2251451/93‑1 (MQ=255)

       tttttCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑Ctta                                                                                  <  1:6011111/93‑1 (MQ=255)

           tCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTtattat                                                                              <  2:3206431/93‑1 (MQ=255)

           tCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTtattat                                                                              <  2:8333066/93‑1 (MQ=255)

             gCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTAttt                                                                            <  1:9130963/93‑1 (MQ=255)

               tGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTAc                                                                          >  1:4849344/1‑93 (MQ=255)

               tGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTAc                                                                          <  1:3239747/93‑1 (MQ=255)

                gCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTAcc                                                                         >  2:6245297/1‑93 (MQ=255)

                  gTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAt                                                                       >  2:6970087/1‑93 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAtt                                                                      >  1:6956198/1‑93 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAtt                                                                      <  2:2085011/93‑1 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAtt                                                                      <  2:3946108/93‑1 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAtt                                                                      >  2:687414/1‑93 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAtt                                                                      >  2:4990144/1‑93 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAtt                                                                      <  1:8363075/93‑1 (MQ=255)

                    aCTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCAttt                                                                     <  1:9877790/93‑1 (MQ=255)

                      tGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGa                                                                   >  1:283937/1‑93 (MQ=255)

                       ttATTCTCGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGGTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGAt                                                                  <  1:2391767/92‑1 (MQ=255)

                           tCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACa                                                              >  1:8469107/1‑93 (MQ=255)

                            cATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACaa                                                             >  2:6668971/1‑93 (MQ=255)

                            cATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACaa                                                             >  2:6124492/1‑93 (MQ=255)

                             aTGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACaaa                                                            <  2:6347285/93‑1 (MQ=255)

                             aTGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACaaa                                                            <  1:1175630/93‑1 (MQ=255)

                               gACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATg                                                          <  2:458820/93‑1 (MQ=255)

                               gACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATg                                                          <  1:2026348/93‑1 (MQ=255)

                                aCCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGt                                                         <  2:8235196/93‑1 (MQ=255)

                                 ccTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGtt                                                        >  1:7460882/1‑93 (MQ=255)

                                  cTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGttt                                                       <  2:8027062/93‑1 (MQ=255)

                                   tGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTg                                                      <  2:1861405/93‑1 (MQ=255)

                                   tGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTg                                                      <  2:6518917/93‑1 (MQ=255)

                                     ctccgcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGt                                                    <  2:1219228/93‑1 (MQ=255)

                                      tcctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTc                                                   <  1:7567768/93‑1 (MQ=255)

                                      tcctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTc                                                   >  1:2467261/1‑93 (MQ=255)

                                      tcctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTc                                                   <  1:2649785/93‑1 (MQ=255)

                                       cctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCt                                                  >  2:7968011/1‑93 (MQ=255)

                                       cctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCt                                                  >  1:1495078/1‑93 (MQ=255)

                                       cctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCt                                                  >  2:423724/1‑93 (MQ=255)

                                       cctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCt                                                  <  2:1265708/93‑1 (MQ=255)

                                        ctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCtt                                                 <  1:7256423/93‑1 (MQ=255)

                                        ctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCtt                                                 >  1:6220525/1‑93 (MQ=255)

                                         tcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCttt                                                <  2:6067723/93‑1 (MQ=255)

                                         tcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCttt                                                >  1:6329523/1‑93 (MQ=255)

                                          cACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTc                                               >  1:3750566/1‑93 (MQ=255)

                                          cACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTc                                               <  2:2125934/93‑1 (MQ=255)

                                           aCCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCg                                              <  1:5847036/93‑1 (MQ=255)

                                              tGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGc                                           >  2:8450515/1‑93 (MQ=255)

                                              tGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGc                                           >  1:10014300/1‑93 (MQ=255)

                                              tGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGc                                           >  1:6713510/1‑93 (MQ=255)

                                               gATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGcc                                          <  1:1314302/93‑1 (MQ=255)

                                               gATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGcc                                          >  1:7996990/1‑93 (MQ=255)

                                                aTTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCa                                         <  2:3337985/93‑1 (MQ=255)

                                                aTTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCa                                         <  1:349039/93‑1 (MQ=255)

                                                aTTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCa                                         >  1:1430161/1‑93 (MQ=255)

                                                    aTTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTa                                     >  2:7465958/1‑93 (MQ=255)

                                                    aTTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTa                                     >  1:9344090/1‑93 (MQ=255)

                                                      tttttCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaa                                   >  2:3065006/1‑93 (MQ=255)

                                                      tttttCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaa                                   <  2:9083024/93‑1 (MQ=255)

                                                       ttttCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaa                                  >  1:8611794/1‑93 (MQ=255)

                                                       ttttCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaa                                  >  2:5785340/1‑93 (MQ=255)

                                                        tttctttcTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaaa                                 >  1:8691213/1‑93 (MQ=255)

                                                        tttctttcTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaaa                                 >  2:7398814/1‑93 (MQ=255)

                                                          tctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaaaaa                               <  1:5671724/93‑1 (MQ=255)

                                                          tctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaaaaa                               <  2:177911/93‑1 (MQ=255)

                                                           ctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAc                              >  1:3424976/1‑93 (MQ=255)

                                                           ctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAc                              <  2:3981576/93‑1 (MQ=255)

                                                           ctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAc                              <  2:9451585/93‑1 (MQ=255)

                                                            tttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATGTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAcc                             <  2:5470702/93‑1 (MQ=255)

                                                             ttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCg                            >  1:9165008/1‑93 (MQ=255)

                                                             ttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCg                            <  1:5337362/93‑1 (MQ=255)

                                                              tctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGc                           >  1:1681212/1‑93 (MQ=255)

                                                                tGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGccc                         >  1:3026767/1‑93 (MQ=255)

                                                                tGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGccc                         <  1:6509972/93‑1 (MQ=255)

                                                                tGTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGccc                         >  2:100228/1‑93 (MQ=255)

                                                                 gTTTTGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGcccc                        <  2:4674793/93‑1 (MQ=255)

                                                                  ttttGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCg                       >  2:9490798/1‑93 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     <  2:4807668/93‑1 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     <  1:7892361/93‑1 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     <  1:4718370/93‑1 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     <  1:950752/93‑1 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     <  1:4496731/93‑1 (MQ=255)

                                                                     tGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAg                    >  2:6825058/1‑93 (MQ=255)

                                                                     tGCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAg                    <  1:1270691/93‑1 (MQ=255)

                                                                      tctgtCGTTAAGCATCTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAgg                   <  2:7034000/89‑1 (MQ=255)

                                                                      gCTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAgg                   <  2:8751783/93‑1 (MQ=255)

                                                                       cTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAggg                  <  2:1549157/93‑1 (MQ=255)

                                                                       cTTTCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAggg                  <  2:2602895/93‑1 (MQ=255)

                                                                        tttCGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggc                 >  1:3700640/1‑93 (MQ=255)

                                                                        cttCGTTAAGCAACCTTTGGCTTACCTAATTATTT‑CCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggc                 <  2:9772548/92‑1 (MQ=255)

                                                                           cGTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggcggc              >  1:7693626/1‑93 (MQ=255)

                                                                            gTTAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGct             >  1:522431/1‑93 (MQ=255)

                                                                             ttAAGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGctc            <  2:862990/93‑1 (MQ=255)

                                                                               aaGCATCTTTTGGCTCA‑TTTATTATTTCCTATTGAATAACAAATGTTTGGTTTTTGGGGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTg          <  2:8827349/93‑1 (MQ=255)

                                                                                aGCAACTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGt         >  2:4406482/1‑93 (MQ=255)

                                                                                    aCTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAt     <  1:7151250/93‑1 (MQ=255)

                                                                                    aCTTTTGGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAt     >  1:4300140/1‑93 (MQ=255)

TCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCA‑CTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTC  >  NC_000913/1684582‑1684761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`