BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,693,549	A→G	W183R (TGG→CGG)	uidA ←	beta‑D‑glucuronidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,693,549	0	A	G	99.5%	688.2 / ‑5.7	218	W183R (TGG→CGG)	uidA	beta‑D‑glucuronidase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (111/106); total (111/107)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 231 total aligned reads displayed.

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTT  >  NC_000913/1693459‑1693639

tGACATCACCATTGGCCACCGCCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                          <  2:5146823/93‑1 (MQ=255)

tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                          <  1:9083450/93‑1 (MQ=255)

tGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCggg                                                                                          >  1:7309011/1‑93 (MQ=255)

 gACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgt                                                                                         <  1:366656/93‑1 (MQ=255)

 gACATCACCATTGGCCAACACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCgggg                                                                                         >  2:1059426/1‑92 (MQ=255)

  aCATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGgtg                                                                                        <  1:8832514/93‑1 (MQ=255)

     tCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTc                                                                                     >  1:2990649/1‑93 (MQ=255)

      cACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCg                                                                                    >  1:8146783/1‑93 (MQ=255)

         cATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCg                                                                                 >  2:9396596/1‑93 (MQ=255)

          aTTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgt                                                                                >  1:7196365/1‑93 (MQ=255)

           ttGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtg                                                                               <  1:4993061/93‑1 (MQ=255)

             ggCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCgtggt                                                                             <  1:9604800/93‑1 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                          >  1:172470/1‑93 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                          <  1:6547623/93‑1 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTa                                                                          >  2:6343626/1‑93 (MQ=255)

                caccacCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGGTTTCGGCGTGGTGTa                                                                          >  1:5915450/1‑93 (MQ=255)

                 cccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                         <  2:8264503/92‑1 (MQ=255)

                 accaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTag                                                                         >  2:7566574/1‑93 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                        >  1:1613055/1‑93 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                        >  2:4221949/1‑93 (MQ=255)

                  ccaccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTaga                                                                        <  1:4917854/93‑1 (MQ=255)

                   caccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTagag                                                                       <  2:2221809/93‑1 (MQ=255)

                   caccTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTagac                                                                       >  1:9004496/1‑92 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:8526593/1‑93 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:782211/1‑93 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:4674564/1‑93 (MQ=255)

                       tGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCAtt                                                                   >  1:1247058/1‑93 (MQ=255)

                        gCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTa                                                                  <  1:3113167/93‑1 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               <  2:4790573/93‑1 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               >  1:169362/1‑93 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               <  1:2740528/93‑1 (MQ=255)

                           aGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGc                                                               <  2:5704634/93‑1 (MQ=255)

                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                              >  1:6760169/1‑93 (MQ=255)

                            gTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCt                                                              >  2:2248772/1‑93 (MQ=255)

                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTg                                                             >  1:5141715/1‑93 (MQ=255)

                             tCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTg                                                             >  2:1703656/1‑93 (MQ=255)

                               aaCAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCg                                                           >  1:3386750/1‑93 (MQ=255)

                                 cAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGAt                                                         <  1:9535032/93‑1 (MQ=255)

                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                       <  1:1082820/93‑1 (MQ=255)

                                   gACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATgg                                                       >  1:10008739/1‑93 (MQ=255)

                                    aCGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGa                                                      <  1:7074039/93‑1 (MQ=255)

                                       cgTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGACATCGTCCACCCGGGTGTTCGGCGTGGGGTAGAGCATTACGCTGCGATGGATcc                                                   >  2:1968250/1‑93 (MQ=255)

                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                  <  1:9346165/93‑1 (MQ=255)

                                        gTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATccc                                                  <  2:1845391/93‑1 (MQ=255)

                                         tGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCg                                                 >  1:4122494/1‑93 (MQ=255)

                                         tGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGCGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCg                                                 >  2:4139575/1‑93 (MQ=255)

                                          ggTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCggg                                                >  1:7623825/1‑93 (MQ=255)

                                           gTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGc                                               <  2:8533304/93‑1 (MQ=255)

                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGCTCCCGGCATa                                            >  2:5266591/1‑93 (MQ=255)

                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                            <  2:1222892/93‑1 (MQ=255)

                                              aCAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATa                                            >  2:2864998/1‑93 (MQ=255)

                                               cAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAg                                           <  1:5941496/93‑1 (MQ=255)

                                                aGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGt                                          >  2:1362143/1‑93 (MQ=255)

                                                 gTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGtt                                         >  2:8302456/1‑93 (MQ=255)

                                                   cTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTaa                                       <  1:8767548/93‑1 (MQ=255)

                                                      tcgcgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGa                                    <  1:1568945/92‑1 (MQ=255)

                                                      gcgcGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTTCGCTGCGATGGATCCCGGCATAGTTAAAGa                                    <  2:2316263/93‑1 (MQ=255)

                                                      gcgcGACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTGAAAGa                                    <  2:5727754/93‑1 (MQ=255)

                                                       cgcgACATGCGTCACCACGCTGATACCGACCGCCCGGGTGCCCGGCGTGCTGTAGAGCCTTACGCTGCGATGGATTCCGGCAAAGTTAAAGaa                                   <  2:6994101/93‑1 (MQ=255)

                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                 <  2:704168/93‑1 (MQ=255)

                                                         cgACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAAt                                 <  1:9200129/93‑1 (MQ=255)

                                                          gACATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATc                                >  1:2237669/1‑93 (MQ=255)

                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                              <  2:7295754/93‑1 (MQ=255)

                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                              >  2:5077795/1‑93 (MQ=255)

                                                            cATGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCAt                              <  2:4490139/93‑1 (MQ=255)

                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            >  2:2697146/1‑93 (MQ=255)

                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  2:6267128/93‑1 (MQ=255)

                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  1:9974877/93‑1 (MQ=255)

                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  1:372760/93‑1 (MQ=255)

                                                              tGCGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATgg                            <  2:8836875/93‑1 (MQ=255)

                                                                cGTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGaa                          <  2:9303573/93‑1 (MQ=255)

                                                                 gTCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                         <  1:9694884/93‑1 (MQ=255)

                                                                 gTCACCACGGTGATATCGTCCACCCGGCTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAg                         <  2:6876290/93‑1 (MQ=255)

                                                                  tCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGa                                                          <  2:5222933/59‑1 (MQ=255)

                                                                  tCACCACGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGa                                                          >  1:5222934/1‑59 (MQ=255)

                                                                  tCACCACGGTGATAACGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGt                        >  2:7167144/1‑93 (MQ=255)

                                                                   caccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTa                       >  2:7958147/1‑93 (MQ=255)

                                                                   caccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTa                       <  2:3115951/93‑1 (MQ=255)

                                                                    accacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                      <  2:4042925/93‑1 (MQ=255)

                                                                    accacGGTGATATCGTCCACACGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTaa                      >  2:6370488/1‑93 (MQ=255)

                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                     <  2:1300005/93‑1 (MQ=255)

                                                                     ccacGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAg                     >  2:8194831/1‑93 (MQ=255)

                                                                       acGGTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGAc                   <  1:4252795/93‑1 (MQ=255)

                                                                          gTGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGc                >  1:191697/1‑93 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt               <  1:618933/93‑1 (MQ=255)

                                                                           tGATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCt               <  2:1480522/93‑1 (MQ=255)

                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt              >  1:1419993/1‑93 (MQ=255)

                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt              >  1:379346/1‑93 (MQ=255)

                                                                            gATATCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtt              >  1:316693/1‑93 (MQ=255)

                                                                             atatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCttt             <  2:6002700/93‑1 (MQ=255)

                                                                             atatCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCttt             <  2:1417736/93‑1 (MQ=255)

                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCtttttt          >  1:6027583/1‑92 (MQ=255)

                                                                                tCGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTc          >  2:4529176/1‑93 (MQ=255)

                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt         <  2:4513240/93‑1 (MQ=255)

                                                                                 cGTCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCt         <  2:8688218/93‑1 (MQ=255)

                                                                                   tCCACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTg       >  2:3814912/1‑93 (MQ=255)

                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc     <  2:4760044/93‑1 (MQ=255)

                                                                                     cACCCGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGcc     >  2:9081299/1‑93 (MQ=255)

                                                                                       cccGGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGt   <  1:15013/93‑1 (MQ=255)

                                                                                        ccAGGTGTTCGGCGTGGTGTAGAGCAGTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGtt  <  1:2794574/93‑1 (MQ=255)

TGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTT  >  NC_000913/1693459‑1693639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`