BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,713,565	G→A	H117Y (CAT→TAT)	pdxY ←	pyridoxal kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,713,565	0	G	A	99.6%	872.1 / NA	242	H117Y (CAT→TAT)	pdxY	pyridoxal kinase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (117/124); minor base C (1/0); total (118/124)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTCCG  >  NC_000913/1713475‑1713654

gATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAAc                                                                                         >  2:476972/1‑93 (MQ=255)

gATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAAc                                                                                         <  2:8112140/93‑1 (MQ=255)

gATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAAc                                                                                         >  2:6353635/1‑93 (MQ=255)

gATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAAc                                                                                         >  1:2883598/1‑93 (MQ=255)

 aTTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAAcc                                                                                        <  1:7333538/93‑1 (MQ=255)

 aTTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAAcc                                                                                        <  2:4186899/93‑1 (MQ=255)

   ttGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCa                                                                                      <  1:7168549/93‑1 (MQ=255)

   ttGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCa                                                                                      <  1:9107144/93‑1 (MQ=255)

   ttGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCa                                                                                      <  2:6294630/93‑1 (MQ=255)

      gcgcAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTa                                                                                   >  1:6124805/1‑93 (MQ=255)

       cgcAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTAc                                                                                  <  1:6234108/93‑1 (MQ=255)

         cAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCg                                                                                >  2:2563470/1‑93 (MQ=255)

         cAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCg                                                                                <  1:7696345/93‑1 (MQ=255)

            tGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGAt                                                                             <  2:3785364/93‑1 (MQ=255)

            tGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGAt                                                                             >  2:4636282/1‑93 (MQ=255)

              atatCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCg                                                                           >  1:3465041/1‑93 (MQ=255)

              atatCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTGCCGGATAACCCATTACCGGATCg                                                                           >  2:5532278/1‑93 (MQ=255)

               tatCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGc                                                                          <  1:9067790/93‑1 (MQ=255)

                atCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCa                                                                         >  2:5234048/1‑93 (MQ=255)

                 tCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCaa                                                                        <  2:9287119/93‑1 (MQ=255)

                 tCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCaa                                                                        >  1:445132/1‑93 (MQ=255)

                 tCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCaa                                                                        <  2:9467912/93‑1 (MQ=255)

                   gCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCaaaa                                                                      <  2:3573613/93‑1 (MQ=255)

                    cTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCaaaaa                                                                     >  2:2187887/1‑93 (MQ=255)

                     tGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAat                                                                    >  1:3477742/1‑93 (MQ=255)

                      ggcaggcaAACCGTGCCGCACATGAAACTCTGCGACTCCCGGTGCAACGATACAGCCTTTTTCCGTATAACCCATTACCGGATCGCAAAAata                                                                   <  2:8530122/93‑1 (MQ=255)

                      ggcaggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAata                                                                   >  2:7527186/1‑93 (MQ=255)

                      ggcaggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAata                                                                   <  1:1233844/93‑1 (MQ=255)

                       gcaggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAatat                                                                  <  2:775280/93‑1 (MQ=255)

                        caggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATAtt                                                                 <  1:4507501/93‑1 (MQ=255)

                         aggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATAttt                                                                <  1:4958708/93‑1 (MQ=255)

                         aggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATAttt                                                                <  2:5955562/93‑1 (MQ=255)

                         aggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATAttt                                                                <  1:5125980/93‑1 (MQ=255)

                         aggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATAttt                                                                >  2:4297238/1‑93 (MQ=255)

                          ggcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTc                                                               >  1:6042367/1‑93 (MQ=255)

                           gcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCg                                                              >  1:9814723/1‑93 (MQ=255)

                           gcaAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCg                                                              >  1:8824004/1‑93 (MQ=255)

                            caAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGc                                                             <  1:9384450/93‑1 (MQ=255)

                            caAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGc                                                             <  2:1830923/93‑1 (MQ=255)

                             aaaCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGcc                                                            <  2:1300661/93‑1 (MQ=255)

                             aaaCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGcc                                                            <  1:1779620/93‑1 (MQ=255)

                              agccGTGCCGCACATGAAACTCTGCAACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCt                                                           <  2:6187319/91‑1 (MQ=255)

                              aaCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCt                                                           <  1:10020624/93‑1 (MQ=255)

                              aaCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCt                                                           <  1:3923914/93‑1 (MQ=255)

                               aCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTg                                                          <  1:4734236/93‑1 (MQ=255)

                               aCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTg                                                          >  2:9590112/1‑93 (MQ=255)

                               aCCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTg                                                          >  2:483302/1‑93 (MQ=255)

                                 cGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCg                                                        <  2:2306859/93‑1 (MQ=255)

                                   tGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGa                                                      >  1:949407/1‑93 (MQ=255)

                                   tGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGa                                                      <  2:445132/93‑1 (MQ=255)

                                    gCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGAt                                                     >  1:672420/1‑93 (MQ=255)

                                      cGCACATGAAACTCTGCGACGCCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTc                                                   <  2:6709850/93‑1 (MQ=255)

                                       gCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTcg                                                  >  1:7449880/1‑93 (MQ=255)

                                        gacaTGCACCTCTCCGGCCCCCGGTGCAACGATACCGCCTTTTTCCGGATCACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTcgc                                                 <  2:3173492/92‑1 (MQ=255)

                                          cgtGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCgg                                               >  2:7027164/3‑93 (MQ=255)

                                           aTGAACCTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGc                                              <  1:7123777/93‑1 (MQ=255)

                                           aTGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGc                                              >  1:7828741/1‑93 (MQ=255)

                                            tGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCt                                             >  1:5485913/1‑93 (MQ=255)

                                            tGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCt                                             >  2:3419611/1‑93 (MQ=255)

                                            tGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCt                                             >  1:5255922/1‑93 (MQ=255)

                                             gAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCtt                                            >  2:2447736/1‑93 (MQ=255)

                                              aaaCTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCttt                                           <  2:4999037/93‑1 (MQ=255)

                                               aaCTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTc                                          >  2:9418003/1‑93 (MQ=255)

                                               aaCTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTc                                          >  1:8750348/1‑93 (MQ=255)

                                                aCTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCa                                         >  2:9142069/1‑93 (MQ=255)

                                                aCTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCa                                         <  2:2792724/93‑1 (MQ=255)

                                                     gCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTga                                    <  1:3260094/93‑1 (MQ=255)

                                                      cGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgac                                   <  1:8795220/93‑1 (MQ=255)

                                                      cGACACCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgac                                   >  2:8737287/1‑93 (MQ=255)

                                                         cacCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgacgga                                >  2:3332418/1‑93 (MQ=255)

                                                         cacCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgacgga                                >  1:1532407/1‑93 (MQ=255)

                                                          acCCGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgacggac                               >  1:6671861/1‑93 (MQ=255)

                                                           cccGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgacggacg                              >  2:1626526/1‑93 (MQ=255)

                                                           cccGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTgacggacg                              <  1:8241042/93‑1 (MQ=255)

                                                            ccGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGCCGa                             >  1:8620437/1‑93 (MQ=255)

                                                            ccGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGa                             <  2:8841967/93‑1 (MQ=255)

                                                            ccGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGa                             <  1:274562/93‑1 (MQ=255)

                                                             cGGTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGAt                            >  2:2863434/1‑93 (MQ=255)

                                                              ggTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATa                           >  1:2401532/1‑93 (MQ=255)

                                                              ggTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATa                           >  1:4077538/1‑93 (MQ=255)

                                                              agTGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATa                           <  1:596611/92‑1 (MQ=255)

                                                                tGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATAcc                         >  2:7242424/1‑93 (MQ=255)

                                                                tGCAACGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATAcc                         >  2:7903115/1‑93 (MQ=255)

                                                                   aaCGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAg                      <  1:901618/93‑1 (MQ=255)

                                                                     cGATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGa                    <  1:9120991/93‑1 (MQ=255)

                                                                      gATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGaa                   >  2:1110718/1‑92 (MQ=255)

                                                                      gATACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATAACGAGGat                   >  1:3942662/1‑93 (MQ=255)

                                                                       aTACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGata                  <  1:9786449/93‑1 (MQ=255)

                                                                       aTACAGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGata                  <  2:5770721/93‑1 (MQ=255)

                                                                         aCAGCCTTTTTCCGGATAACCCATTACCGAATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATg                >  1:431656/1‑93 (MQ=255)

                                                                           aGCCTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGtt              <  2:405660/93‑1 (MQ=255)

                                                                             ccTTTTTCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCa            <  2:9733037/93‑1 (MQ=255)

                                                                               tttttCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCAcc          >  2:1699095/1‑93 (MQ=255)

                                                                                ttttCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCAccc         <  2:7491017/93‑1 (MQ=255)

                                                                                ttttCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCAccc         <  2:5881551/93‑1 (MQ=255)

                                                                                  ttCCGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTg       >  1:2196948/1‑93 (MQ=255)

                                                                                    ccGGATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCt     <  1:4569769/93‑1 (MQ=255)

                                                                                      ggATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTcc   <  2:8376911/93‑1 (MQ=255)

                                                                                      ggATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTcc   <  2:3525759/93‑1 (MQ=255)

                                                                                       gATAACCCATTACCGGATCGCAAAAATATTTCGCCTGCTGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTccg  <  2:6591710/93‑1 (MQ=255)

GATTTGGCGCAATGATATCGCTGGCAGGCAAACCGTGCCGCACATGAAACTCTGCGACACCCGGTGCAACGATACAGCCTTTTTCCGGATGACCCATTACCGGATCGCAAAAATATTTCGCCTGCGGATTCGCGGCTTTCACCTGACGGACGATACCGAGGATATGTTCACCCTGCTCCG  >  NC_000913/1713475‑1713654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`