BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,150	A→G	T88T (ACT→ACC)	ydhA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,150	0	A	G	99.2%	839.8 / ‑5.1	265	T88T (ACT→ACC)	ydhA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (162/101); total (162/103)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 278 total aligned reads displayed.

GACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG  >  NC_000913/1716059‑1716239

 aCGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGt                                                                                         <  1:5792793/93‑1 (MQ=255)

  cGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTc                                                                                        >  1:4557994/1‑93 (MQ=255)

   gCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCg                                                                                       >  2:1047339/1‑93 (MQ=255)

    cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                      >  2:4510732/1‑93 (MQ=255)

    cTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGc                                                                                      <  1:1938441/93‑1 (MQ=255)

     tATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCt                                                                                     >  1:2625129/1‑93 (MQ=255)

     tATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCt                                                                                     <  2:8026705/93‑1 (MQ=255)

      aTTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCtt                                                                                    <  2:3455917/93‑1 (MQ=255)

       ttGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTc                                                                                   >  2:3294723/1‑93 (MQ=255)

        tgtgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGATAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCa                                                                                  >  2:3724039/1‑93 (MQ=255)

        tgtgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCa                                                                                  <  1:6277276/93‑1 (MQ=255)

          tgCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                                <  2:862447/93‑1 (MQ=255)

          gccgccgcCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATc                                                                                <  2:436592/91‑1 (MQ=255)

             gccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGcc                                                                             >  2:5659182/1‑93 (MQ=255)

             gccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGcc                                                                             <  2:4562465/93‑1 (MQ=255)

              ccgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTGAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCt                                                                            >  1:1918022/1‑93 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           <  2:7653955/93‑1 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           >  2:6566871/1‑93 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           >  1:9421253/1‑93 (MQ=255)

               cgccCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCtt                                                                           >  1:9037092/1‑93 (MQ=255)

                cccctTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCttt                                                                          <  2:5671687/92‑1 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         <  1:8105756/93‑1 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         <  2:6406155/93‑1 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         >  1:5389476/1‑93 (MQ=255)

                 ccccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTc                                                                         >  1:5059120/1‑93 (MQ=255)

                  cccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCg                                                                        <  1:9534708/93‑1 (MQ=255)

                   ccTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGa                                                                       <  2:8597429/93‑1 (MQ=255)

                    cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  2:3209490/1‑93 (MQ=255)

                    cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      <  1:382245/93‑1 (MQ=255)

                    cTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGAc                                                                      >  1:839038/1‑93 (MQ=255)

                         aaaaTCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGaa                                                                 >  2:9022523/1‑93 (MQ=255)

                                 aaCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGa                                                         >  2:5088266/1‑93 (MQ=255)

                                  aCGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAt                                                        >  2:6367484/1‑93 (MQ=255)

                                   cGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGAtt                                                       <  2:8748407/93‑1 (MQ=255)

                                    gCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACACAGATTc                                                      >  2:5758541/1‑93 (MQ=255)

                                      tgtgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCg                                                    >  1:1052836/1‑93 (MQ=255)

                                        tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  <  2:688830/93‑1 (MQ=255)

                                        tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  >  2:823192/1‑93 (MQ=255)

                                        tgGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTAGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTc                                                  >  2:4062564/1‑93 (MQ=255)

                                          gATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAg                                                >  1:4758138/1‑93 (MQ=255)

                                          gATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAg                                                >  1:2313961/1‑93 (MQ=255)

                                            ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                              >  2:1929637/1‑93 (MQ=255)

                                            ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                              <  1:2772541/93‑1 (MQ=255)

                                            ttttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtg                                              >  2:8708713/1‑93 (MQ=255)

                                             tttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtgt                                             <  2:6668444/93‑1 (MQ=255)

                                             tttGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAgtgt                                             <  2:682240/93‑1 (MQ=255)

                                               tGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTaa                                           >  1:2994732/1‑93 (MQ=255)

                                                    cTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAAcgcgc                                      >  1:9322290/1‑93 (MQ=255)

                                                      gACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACgcgcgc                                    >  2:1748352/1‑93 (MQ=255)

                                                       aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   >  2:1218548/1‑93 (MQ=255)

                                                       aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   >  1:858832/1‑93 (MQ=255)

                                                       aCAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGcc                                   <  1:7781081/93‑1 (MQ=255)

                                                        cAGTTATTCAAGACGATGCGGTTGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                  <  1:7224909/93‑1 (MQ=255)

                                                        cAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                  <  2:4347501/93‑1 (MQ=255)

                                                        cAGTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCa                                  <  2:2033312/93‑1 (MQ=255)

                                                          gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaga                                >  2:8604059/1‑93 (MQ=255)

                                                          gTTATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCaca                                >  2:2307638/1‑91 (MQ=255)

                                                           ttATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCagag                               <  2:7256870/93‑1 (MQ=255)

                                                            tATTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAgg                              >  2:251179/1‑93 (MQ=255)

                                                             aTTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGc                             >  1:4304425/1‑93 (MQ=255)

                                                             aTTCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGc                             <  1:3303610/93‑1 (MQ=255)

                                                              ttCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCt                            >  1:1341514/1‑93 (MQ=255)

                                                               tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                           >  2:6274711/1‑93 (MQ=255)

                                                               tCAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTg                           >  2:538803/1‑93 (MQ=255)

                                                                cAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                          <  2:6074150/93‑1 (MQ=255)

                                                                cAAGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                          >  1:3619833/1‑93 (MQ=255)

                                                                cAAGACGAAGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGa                          <  1:8768820/93‑1 (MQ=255)

                                                                 aaGACGATGCGGTCGCGTTTATAGAGGGCCGCTTCATCCCCTTTCGCCCTGAAAACATAGAGTCCGTCAGTGTAACGCGCGCCAGAGGCtaca                         >  2:3245208/1‑90 (MQ=255)

                                                                 aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                         >  2:9635726/1‑93 (MQ=255)

                                                                 aaGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaa                         >  1:5603956/1‑93 (MQ=255)

                                                                  aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                        <  1:767417/93‑1 (MQ=255)

                                                                  aGACGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGaaa                        <  2:1265187/93‑1 (MQ=255)

                                                                    aCGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATg                      >  1:989698/1‑93 (MQ=255)

                                                                     cGATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGc                     >  1:7056902/1‑93 (MQ=255)

                                                                      gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                    <  1:6433060/93‑1 (MQ=255)

                                                                      gATGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGcc                    <  1:5920247/93‑1 (MQ=255)

                                                                       aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   >  2:9690271/1‑93 (MQ=255)

                                                                       aTGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGccc                   <  1:9861908/93‑1 (MQ=255)

                                                                        tGCGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCt                  >  1:4388869/1‑93 (MQ=255)

                                                                          cGGTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGt                >  1:8531063/1‑93 (MQ=255)

                                                                           ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt               <  2:3781489/93‑1 (MQ=255)

                                                                           ggTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGtt               <  1:6807308/93‑1 (MQ=255)

                                                                           ggTCGCGTTGATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGGAATGCCCTGtt               <  1:4450160/93‑1 (MQ=255)

                                                                            gTCGCGTTTATAGACGGTCGCTTTATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              >  2:9270118/1‑93 (MQ=255)

                                                                            gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              <  2:7472036/93‑1 (MQ=255)

                                                                            gTCGCGTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGttt              >  2:8449814/1‑93 (MQ=255)

                                                                              cgcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTga            <  2:2879103/93‑1 (MQ=255)

                                                                              cgcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCACTGTTTga            >  1:8193249/1‑93 (MQ=255)

                                                                               gcgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgag           >  1:7501435/1‑93 (MQ=255)

                                                                                cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          >  1:3085436/1‑93 (MQ=255)

                                                                                cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          >  1:5873481/1‑93 (MQ=255)

                                                                                cgTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTgaga          <  2:2710419/93‑1 (MQ=255)

                                                                                 gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt         >  2:9477127/1‑93 (MQ=255)

                                                                                 gTTTATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGAt         <  1:4102460/93‑1 (MQ=255)

                                                                                  tttATAGAGGGGTGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATg        <  2:7847324/93‑1 (MQ=255)

                                                                                  tttATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATg        >  1:1116763/1‑93 (MQ=255)

                                                                                   ttATAGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGc       >  1:1597984/1‑93 (MQ=255)

                                                                                      taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt    <  1:6245574/93‑1 (MQ=255)

                                                                                      taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCagt    >  1:9761584/1‑93 (MQ=255)

                                                                                      taGACGGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCaga    >  1:3439449/1‑92 (MQ=255)

GACGCTATTGTGCGCCGCCCCTGGAAAAATCTCAACGCTGTGGATTTTGTAACTGACAGTTATTCAAGACGATGCGGTCGCGTTTATAGACAGTCGCTTCATCGCCTTTCGACCAGAAAACATAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAG  >  NC_000913/1716059‑1716239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`