BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,749,776	T→C	A693A (GCA→GCG)	b1673 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,749,776	0	T	C	99.3%	879.9 / ‑3.2	274	A693A (GCA→GCG)	b1673	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (155/117); total (157/117)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 293 total aligned reads displayed.

CGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTT  >  NC_000913/1749686‑1749865

 gCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTgccgcc                                                                                        <  2:455292/93‑1 (MQ=255)

 gCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTgccgcc                                                                                        >  2:3852752/1‑93 (MQ=255)

 gCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTgccgcc                                                                                        >  2:1005410/1‑93 (MQ=255)

 gCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTgccgcc                                                                                        >  1:9138186/1‑93 (MQ=255)

   cTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAg                                                                                      >  1:7102271/1‑93 (MQ=255)

    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     >  1:4230655/1‑93 (MQ=255)

    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     >  2:891607/1‑93 (MQ=255)

    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     >  2:7245889/1‑93 (MQ=255)

    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     <  1:3243916/93‑1 (MQ=255)

     cGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGAt                                                                                    <  1:4067885/93‑1 (MQ=255)

     cGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGAt                                                                                    <  1:9394638/93‑1 (MQ=255)

      gTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATc                                                                                   >  1:2563336/1‑93 (MQ=255)

        cAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcgg                                                                                 >  1:2739754/1‑93 (MQ=255)

        cAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcgg                                                                                 <  2:4260391/93‑1 (MQ=255)

         aaCTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcggc                                                                                >  1:7302245/1‑93 (MQ=255)

         aaCTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcggc                                                                                >  1:3457811/1‑93 (MQ=255)

          aCTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcggcg                                                                               >  1:7516411/1‑93 (MQ=255)

          aCTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcggcg                                                                               >  2:8128520/1‑93 (MQ=255)

            ttCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATaggcgga                                                                             >  2:2325803/1‑92 (MQ=255)

             tCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCa                                                                            <  2:4331861/93‑1 (MQ=255)

             tCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCa                                                                            >  2:5997426/1‑93 (MQ=255)

             tCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCa                                                                            <  2:6084643/93‑1 (MQ=255)

              cGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCaa                                                                           >  2:1931240/1‑93 (MQ=255)

              cGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCaa                                                                           <  1:5712101/93‑1 (MQ=255)

                cTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAata                                                                         <  2:5649349/93‑1 (MQ=255)

                cTGACTTTCGCTAACGGCAATTCATCCCGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAata                                                                         <  1:9736465/93‑1 (MQ=255)

                 tGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAatat                                                                        <  1:5954813/93‑1 (MQ=255)

                  gACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATc                                                                       >  2:4178451/1‑93 (MQ=255)

                  gACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATc                                                                       <  1:3982269/93‑1 (MQ=255)

                  gACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATc                                                                       >  2:9211878/1‑93 (MQ=255)

                   aCTTGCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCt                                                                      <  2:2752034/93‑1 (MQ=255)

                    cTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCtt                                                                     >  2:4767354/1‑93 (MQ=255)

                    cTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCtt                                                                     <  2:6328652/93‑1 (MQ=255)

                     tttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTc                                                                    >  2:9267695/1‑93 (MQ=255)

                     tttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTc                                                                    <  1:4593545/93‑1 (MQ=255)

                      ttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTcc                                                                   <  1:3557046/93‑1 (MQ=255)

                      ttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTcc                                                                   <  1:6011758/93‑1 (MQ=255)

                       tCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCa                                                                  <  2:619427/93‑1 (MQ=255)

                       tCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCa                                                                  >  1:3666906/1‑93 (MQ=255)

                       tCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCa                                                                  <  1:7928194/93‑1 (MQ=255)

                       tCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCa                                                                  >  2:4972502/1‑93 (MQ=255)

                         gCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGc                                                                <  2:5907377/93‑1 (MQ=255)

                           tAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGccc                                                              >  1:7193046/1‑93 (MQ=255)

                           tAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGccc                                                              >  2:7006684/1‑93 (MQ=255)

                           tAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGccc                                                              <  1:895061/93‑1 (MQ=255)

                            aaCGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGcccc                                                             >  2:9057251/1‑93 (MQ=255)

                            aaCGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGcccc                                                             >  1:4898221/1‑93 (MQ=255)

                            aaCGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGcccc                                                             >  1:6360151/1‑93 (MQ=255)

                             aCGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCa                                                            >  2:8826784/1‑93 (MQ=255)

                               ggCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGa                                                          <  1:299491/93‑1 (MQ=255)

                                gCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGAc                                                         <  2:667138/93‑1 (MQ=255)

                                 cAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACg                                                        >  1:8311890/1‑93 (MQ=255)

                                 cAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACg                                                        <  1:1820777/93‑1 (MQ=255)

                                   aTTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCt                                                      <  1:3600060/93‑1 (MQ=255)

                                     tCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGc                                                    <  1:6869756/93‑1 (MQ=255)

                                        tCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAAt                                                 >  1:1439613/1‑93 (MQ=255)

                                         ccTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATg                                                <  2:2979059/93‑1 (MQ=255)

                                          cTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGt                                               >  2:4023039/1‑93 (MQ=255)

                                          cTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGt                                               <  1:1155990/93‑1 (MQ=255)

                                            gATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGttt                                             <  2:9412094/93‑1 (MQ=255)

                                             aTGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTc                                            <  2:6697658/93‑1 (MQ=255)

                                              tGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTcg                                           >  1:3743124/1‑93 (MQ=255)

                                               ggTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTcgc                                          >  1:4769663/1‑93 (MQ=255)

                                                  tCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTGCAGCCCCAGACGCTGCAATGTTTCGCggg                                       >  1:1970854/1‑93 (MQ=255)

                                                    aTCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGtt                                     >  1:2152466/1‑93 (MQ=255)

                                                        tACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGAc                                 <  2:5318774/93‑1 (MQ=255)

                                                         aCCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACa                                >  1:7694576/1‑93 (MQ=255)

                                                           ccATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAAc                              <  2:3062518/93‑1 (MQ=255)

                                                            cATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAAcc                             <  2:8387858/93‑1 (MQ=255)

                                                              ttgcTTACGCAGGCAGTAGATTGAGTGCCGCCAGATCGACGGCAATATCCTACGGCCCCCGGCGCTGCAATGTTTCGCAGGTTGGGCAACTaa                           >  2:1900124/4‑93 (MQ=255)

                                                              ttCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACCACCaa                           >  1:5272129/1‑93 (MQ=255)

                                                              ttCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCaa                           <  2:3761208/93‑1 (MQ=255)

                                                               tCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAg                          >  2:1138730/1‑93 (MQ=255)

                                                                ccTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGc                         >  1:1006638/1‑93 (MQ=255)

                                                                 cTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCt                        <  1:1572944/93‑1 (MQ=255)

                                                                  ttACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtg                       >  2:7142149/1‑93 (MQ=255)

                                                                  ttACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtg                       <  1:8090920/93‑1 (MQ=255)

                                                                  ttACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtg                       <  2:153352/93‑1 (MQ=255)

                                                                  ttACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtg                       >  2:1745146/1‑93 (MQ=255)

                                                                  ttACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtg                       >  1:7671026/1‑93 (MQ=255)

                                                                    aCGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtgtg                     <  1:121343/93‑1 (MQ=255)

                                                                        aggcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTc                 >  2:9498261/1‑93 (MQ=255)

                                                                        aggcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTc                 <  2:4691841/93‑1 (MQ=255)

                                                                          gcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTccc               >  2:541533/1‑93 (MQ=255)

                                                                          gcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTccc               >  2:7869772/1‑93 (MQ=255)

                                                                            agTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAg             >  1:6789901/1‑93 (MQ=255)

                                                                             gTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGc            >  2:588331/1‑93 (MQ=255)

                                                                             gTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGc            >  1:774871/1‑93 (MQ=255)

                                                                             gTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGc            <  1:9681115/93‑1 (MQ=255)

                                                                              tAGATTGTGTGCCGCCAGATCGGCGGCAATATGTTCCAGCCCCAGACGCTGGAATGTTTCGCGGGTTGGACAACCCAGCTGTGGGTCCCAGcc           >  1:1344052/1‑93 (MQ=255)

                                                                              tAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGcc           <  2:2115336/93‑1 (MQ=255)

                                                                              tAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGcc           >  1:9979244/1‑93 (MQ=255)

                                                                                gATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGccct         >  1:8597372/1‑92 (MQ=255)

                                                                                gATTGTGCGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCa         >  1:7871858/1‑93 (MQ=255)

                                                                                 aTTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCAt        >  1:8266635/1‑93 (MQ=255)

                                                                                 aTTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCAt        <  2:1403962/93‑1 (MQ=255)

                                                                                  ttGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCAtt       <  1:8830999/93‑1 (MQ=255)

                                                                                     tgtgCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCt    >  1:2461898/1‑93 (MQ=255)

                                                                                      gtgCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCtt   <  2:3416074/93‑1 (MQ=255)

                                                                                       tgccgcCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCttt  >  2:3209660/1‑93 (MQ=255)

CGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTT  >  NC_000913/1749686‑1749865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`