BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,491	T→C	Y455C (TAC→TGC)	b1673 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,491	0	T	C	99.7%	945.1 / ‑5.0	293	Y455C (TAC→TGC)	b1673	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (134/157); total (135/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 299 total aligned reads displayed.

ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA  >  NC_000913/1750401‑1750579

aCAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAc                                                                                        <  2:8741267/93‑1 (MQ=255)

  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCActc                                                                                      <  2:2813315/93‑1 (MQ=255)

  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGCGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCActc                                                                                      >  1:8399731/1‑93 (MQ=255)

     ggAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTc                                                                                   <  2:4094786/93‑1 (MQ=255)

      gAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCa                                                                                  <  2:9566669/93‑1 (MQ=255)

          cGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCa                                                                              <  2:1032125/93‑1 (MQ=255)

           gACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCAAAACGGCGACCAGAGATTATTTTTCGCGTAGGCCCAGCACTCTTCACCCaa                                                                             >  2:7056070/1‑93 (MQ=255)

            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                            <  1:1100416/93‑1 (MQ=255)

            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                            >  1:1713608/1‑93 (MQ=255)

             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                           >  1:1587964/1‑93 (MQ=255)

             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                           <  2:3733063/93‑1 (MQ=255)

              cTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAtt                                                                          <  1:1194755/93‑1 (MQ=255)

              cTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAtt                                                                          <  1:3771105/93‑1 (MQ=255)

               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                         >  1:3381568/1‑93 (MQ=255)

               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                         >  2:6553734/1‑93 (MQ=255)

               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                         >  1:9277692/1‑93 (MQ=255)

                 cgcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCa                                                                       <  1:7063574/93‑1 (MQ=255)

                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                      <  2:5852922/93‑1 (MQ=255)

                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                      <  1:6106559/93‑1 (MQ=255)

                     gccGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATCCCAGCGt                                                                   <  2:6429264/91‑1 (MQ=255)

                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                <  1:9555745/93‑1 (MQ=255)

                         cTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTg                                                               >  2:2465439/1‑93 (MQ=255)

                          ttCATTTGCATGGCGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              <  1:3453291/93‑1 (MQ=255)

                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              >  1:6765652/1‑93 (MQ=255)

                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              >  1:2599300/1‑93 (MQ=255)

                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              <  1:2307179/93‑1 (MQ=255)

                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                              <  2:5163838/93‑1 (MQ=255)

                           tCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCg                                                             >  2:1873686/1‑93 (MQ=255)

                           tCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCg                                                             >  2:1337348/1‑93 (MQ=255)

                            cATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGa                                                            <  2:8320529/93‑1 (MQ=255)

                            cATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGa                                                            <  1:2704192/93‑1 (MQ=255)

                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                           <  1:9106040/93‑1 (MQ=255)

                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                           >  2:3607382/1‑93 (MQ=255)

                               tGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATgg                                                         <  1:4670789/93‑1 (MQ=255)

                                 gCATGGTGAACCGGATAGCGAAACGGCGACCAGAGTTTATTGTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCa                                                       <  1:7342676/93‑1 (MQ=255)

                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCata                                                     <  1:7786545/93‑1 (MQ=255)

                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    <  2:1464156/93‑1 (MQ=255)

                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    >  1:2912715/1‑93 (MQ=255)

                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    >  1:579570/1‑93 (MQ=255)

                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                    >  2:4936717/1‑93 (MQ=255)

                                     ggTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATg                                                   <  2:1711750/93‑1 (MQ=255)

                                       tGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGaa                                                 <  1:3060846/93‑1 (MQ=255)

                                       tGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGaa                                                 <  1:9761771/93‑1 (MQ=255)

                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                              >  1:8274394/1‑93 (MQ=255)

                                           ccGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAt                                             <  2:3309218/93‑1 (MQ=255)

                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                            >  2:7831401/1‑93 (MQ=255)

                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                            >  2:8056072/1‑93 (MQ=255)

                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                           <  1:3177764/93‑1 (MQ=255)

                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                           <  1:3931996/93‑1 (MQ=255)

                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                           >  2:5689059/1‑93 (MQ=255)

                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                          >  2:1408275/1‑93 (MQ=255)

                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                          <  1:5604618/93‑1 (MQ=255)

                                              gATAGCCAAACGGAGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                          <  2:6403330/93‑1 (MQ=255)

                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                         >  1:220519/1‑93 (MQ=255)

                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                         >  2:279507/1‑93 (MQ=255)

                                               aTAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                         <  1:8211820/93‑1 (MQ=255)

                                                tAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                        >  2:7695631/1‑93 (MQ=255)

                                                 aGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                       >  2:5739974/1‑93 (MQ=255)

                                                 aGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                       <  2:238929/93‑1 (MQ=255)

                                                     aaaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                   >  2:7951739/1‑93 (MQ=255)

                                                      aaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                  <  1:8846970/93‑1 (MQ=255)

                                                      aaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                  >  1:854532/1‑93 (MQ=255)

                                                       aCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAc                                 >  2:4294036/1‑93 (MQ=255)

                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                               >  2:4441069/1‑93 (MQ=255)

                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                               <  1:1882653/93‑1 (MQ=255)

                                                            gACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGc                            >  1:5306012/1‑93 (MQ=255)

                                                             aCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCt                           <  2:7530852/93‑1 (MQ=255)

                                                             aCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAACATCAGCCAGGTGACTCAGCt                           >  2:8390542/1‑93 (MQ=255)

                                                              ccAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTc                          >  2:8136537/1‑93 (MQ=255)

                                                               cAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATCCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCa                         <  2:6821225/93‑1 (MQ=255)

                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        >  2:2980726/1‑93 (MQ=255)

                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        >  1:4799806/1‑93 (MQ=255)

                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        <  1:4557251/93‑1 (MQ=255)

                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                        >  1:4155068/1‑93 (MQ=255)

                                                                  agTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAccc                      >  1:2430608/1‑93 (MQ=255)

                                                                  agTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAccc                      >  1:4995428/1‑93 (MQ=255)

                                                                    tttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                    >  1:6448563/1‑93 (MQ=255)

                                                                    tttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                    >  2:4721476/1‑93 (MQ=255)

                                                                      tATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCacac                  <  2:343684/93‑1 (MQ=255)

                                                                      tATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCacac                  >  2:6777009/1‑93 (MQ=255)

                                                                       aTTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACg                 >  1:1790351/1‑93 (MQ=255)

                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  1:9355283/93‑1 (MQ=255)

                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  1:3593367/93‑1 (MQ=255)

                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  2:3130021/93‑1 (MQ=255)

                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                <  2:3806917/93‑1 (MQ=255)

                                                                            tCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATgcg            <  2:3893258/93‑1 (MQ=255)

                                                                            tCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATgcg            <  2:2553515/93‑1 (MQ=255)

                                                                               cgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCa         <  2:5379728/93‑1 (MQ=255)

                                                                                 tAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGa       <  2:2214412/93‑1 (MQ=255)

                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc      <  2:8284960/93‑1 (MQ=255)

                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc      <  1:5701001/93‑1 (MQ=255)

                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc      >  2:9199147/1‑93 (MQ=255)

                                                                                   gCCCCAGCCCTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg     <  1:8742728/93‑1 (MQ=255)

                                                                                    ccccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACgg    <  1:7234323/93‑1 (MQ=255)

                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   <  2:174036/93‑1 (MQ=255)

                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   >  1:7634104/1‑93 (MQ=255)

                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   <  1:4512347/93‑1 (MQ=255)

                                                                                     cccAGAACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGTACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCGCGACggg   >  2:5484935/1‑92 (MQ=255)

                                                                                     cacAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt   <  1:4344098/91‑1 (MQ=255)

                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta  <  2:6268199/93‑1 (MQ=255)

ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA  >  NC_000913/1750401‑1750579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`