BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,749	A→G	I10V (ATC→GTC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,749	0	A	G	99.4%	1046.1 / ‑3.4	322	I10V (ATC→GTC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (158/162); total (159/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 329 total aligned reads displayed.

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTc                                                                                         <  1:7883409/93‑1 (MQ=255)

 ccGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCg                                                                                        <  1:1132905/93‑1 (MQ=255)

 ccGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCg                                                                                        <  1:8563374/93‑1 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      <  2:9773316/93‑1 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      <  2:5770876/93‑1 (MQ=255)

   ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGa                                                                                      >  1:1676969/1‑93 (MQ=255)

     cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAcc                                                                                    <  2:6427881/93‑1 (MQ=255)

     cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGAcc                                                                                    <  2:3385397/93‑1 (MQ=255)

      aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCg                                                                                   <  2:8463102/93‑1 (MQ=255)

       gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGa                                                                                  <  2:4935959/93‑1 (MQ=255)

       gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGa                                                                                  >  2:87804/1‑93 (MQ=255)

           gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAc                                                                              <  2:3679013/93‑1 (MQ=255)

            cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAAcc                                                                             <  2:4066365/93‑1 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           >  2:2716900/1‑93 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           <  1:8047303/93‑1 (MQ=255)

              cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGa                                                                           <  1:5375071/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGCCTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          <  1:3818974/93‑1 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  2:5257610/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  1:52618/1‑93 (MQ=255)

               aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGaa                                                                          >  1:5041734/1‑93 (MQ=255)

                gAAAGCAATTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         <  2:9299811/93‑1 (MQ=255)

                gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAAt                                                                         >  1:3168490/1‑93 (MQ=255)

                 aaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATc                                                                        <  1:7482640/93‑1 (MQ=255)

                  aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCt                                                                       <  2:9106216/93‑1 (MQ=255)

                   aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTg                                                                      >  2:2916488/1‑93 (MQ=255)

                   aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTg                                                                      <  1:1856195/93‑1 (MQ=255)

                      aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAag                                                                   <  2:3510583/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  2:5555883/93‑1 (MQ=255)

                         tttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAgaga                                                                <  1:118613/93‑1 (MQ=255)

                           tctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATg                                                              <  1:4348554/93‑1 (MQ=255)

                              cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                           <  2:3175543/93‑1 (MQ=255)

                              cccATCCTTCTCAACCTAAAGACTAAGACTGTCATGAAAAAGCCCAAAATTGTTTGCACCGCCGGACCGAAAACCTAATCTGAAGAGATGtca                                                           >  1:1410766/1‑91 (MQ=255)

                               ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAg                                                          <  2:4548638/93‑1 (MQ=255)

                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         >  1:636970/1‑93 (MQ=255)

                                cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                         <  1:3106936/93‑1 (MQ=255)

                                  tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGACAACCGAATCTCACGAGATGTTAGCTa                                                       >  1:4967431/1‑93 (MQ=255)

                                   ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaa                                                      <  1:227680/93‑1 (MQ=255)

                                   ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaa                                                      >  2:5181078/1‑93 (MQ=255)

                                     ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                    <  1:9058346/93‑1 (MQ=255)

                                      tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                   >  1:5555520/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  2:6890621/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  2:1953144/1‑93 (MQ=255)

                                       ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                  >  1:4862953/1‑93 (MQ=255)

                                        tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATgt                                                 >  2:2603480/1‑92 (MQ=255)

                                         cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                <  1:2934538/93‑1 (MQ=255)

                                          aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                               >  2:4821191/1‑93 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  2:2222417/1‑93 (MQ=255)

                                           aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                                              >  2:6608433/1‑93 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  2:4371424/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  1:399389/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  2:1491661/93‑1 (MQ=255)

                                            cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGa                                             <  1:2537166/93‑1 (MQ=255)

                                             ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGAc                                            >  1:4116419/1‑93 (MQ=255)

                                               agagactaagactGTAATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACGGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          <  2:10744/91‑1 (MQ=255)

                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          >  2:7294145/1‑93 (MQ=255)

                                               aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                          <  1:5020080/93‑1 (MQ=255)

                                                aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                                         <  1:6034750/93‑1 (MQ=255)

                                                 agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                        <  2:4015939/93‑1 (MQ=255)

                                                  gactaagactGTCATGAAAAAGACCAGAATTGTATGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       <  2:8962672/93‑1 (MQ=255)

                                                  gactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                       >  1:1564852/1‑93 (MQ=255)

                                                    ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                     >  2:1244499/1‑93 (MQ=255)

                                                    ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                     >  1:9469307/1‑93 (MQ=255)

                                                    ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                     <  1:6857576/93‑1 (MQ=255)

                                                     taagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                    <  2:6228431/93‑1 (MQ=255)

                                                      aagactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATg                                   <  2:5867202/93‑1 (MQ=255)

                                                        gactGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGaa                                 <  1:1058951/93‑1 (MQ=255)

                                                         actGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                <  1:1485534/93‑1 (MQ=255)

                                                          ctGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                               >  1:6247376/1‑93 (MQ=255)

                                                           tGTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                              >  1:7961912/1‑93 (MQ=255)

                                                            gTCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                             >  1:985844/1‑93 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:4480098/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:7056012/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            <  1:8414020/93‑1 (MQ=255)

                                                             tCATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa                            >  1:2724259/1‑93 (MQ=255)

                                                              cATGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                           >  2:6011869/1‑93 (MQ=255)

                                                               aTGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATg                          <  2:1256921/93‑1 (MQ=255)

                                                                tGAAAAAGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                         >  2:7626411/1‑93 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:9711674/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:7927949/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      <  2:4305961/93‑1 (MQ=255)

                                                                   aaaaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                      >  2:8616226/1‑93 (MQ=255)

                                                                      aGACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGa                   <  2:7376369/93‑1 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:8119442/1‑93 (MQ=255)

                                                                       gACCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                  >  1:2462539/1‑93 (MQ=255)

                                                                        aCCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaat                 >  1:7656043/1‑92 (MQ=255)

                                                                        aCCAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAc                 >  2:1164970/1‑93 (MQ=255)

                                                                         ccAAAATTGTTTGCACCGTCGGACCGAAAACCGCATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGGCTGAACt                >  1:7223385/1‑93 (MQ=255)

                                                                         ccAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACt                >  1:3411250/1‑93 (MQ=255)

                                                                          cAAAATTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACtc               >  1:5666850/1‑92 (MQ=255)

                                                                           aaaaTTGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc              >  2:7188312/1‑93 (MQ=255)

                                                                              aatGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGCACTtctct           >  1:9183604/3‑93 (MQ=255)

                                                                               ttGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGCCGCTGGCATGAAACTTATGCGTCTGAACTtctctc          <  1:1815453/93‑1 (MQ=255)

                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         <  1:572554/93‑1 (MQ=255)

                                                                                tGTTTGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa         >  2:5739919/1‑93 (MQ=255)

                                                                                 gTTTGCACCGTCGTACCGAAAACCGAATCTGAATAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt        >  2:7699927/1‑93 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  2:8086733/93‑1 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  1:8820856/93‑1 (MQ=255)

                                                                                  tttGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATg       <  1:216216/93‑1 (MQ=255)

                                                                                    tGCACCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt     >  2:3743660/1‑93 (MQ=255)

                                                                                       aCCGTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAc  <  2:3970882/93‑1 (MQ=255)

TCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGAC  >  NC_000913/1753659‑1753838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`