BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	198,418	A→G	D164G (GAC→GGC)	yaeT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	198,418	0	A	G	99.8%	1275.7 / ‑5.6	394	D164G (GAC→GGC)	yaeT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (191/201); total (191/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 400 total aligned reads displayed.

AGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGA  >  NC_000913/198328‑198507

agaAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGcc                                                                                         >  1:6044618/1‑93 (MQ=255)

    aGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTaaa                                                                                     >  2:5484930/1‑93 (MQ=255)

     ggTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTaaaa                                                                                    <  1:4977893/93‑1 (MQ=255)

     ggTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTaaaa                                                                                    <  1:4926504/93‑1 (MQ=255)

     ggTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTaaaa                                                                                    >  1:4364816/1‑93 (MQ=255)

         tGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTgg                                                                                >  2:7072389/1‑93 (MQ=255)

           gAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGgtg                                                                              >  1:8527865/1‑93 (MQ=255)

           gAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGgtg                                                                              >  2:1244565/1‑93 (MQ=255)

            aaGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGgtgt                                                                             <  2:4043640/93‑1 (MQ=255)

            aaGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGgtgt                                                                             <  1:3572157/93‑1 (MQ=255)

              gACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTc                                                                           >  1:6728103/1‑93 (MQ=255)

               aCTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTcc                                                                          <  1:581358/93‑1 (MQ=255)

                cTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCa                                                                         >  2:8026974/1‑93 (MQ=255)

                 ttCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAg                                                                        >  2:3735955/1‑93 (MQ=255)

                  tCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAgg                                                                       >  1:2884203/1‑93 (MQ=255)

                   ctactaCAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGa                                                                      >  2:9301925/1‑93 (MQ=255)

                   ctactaCAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGa                                                                      >  1:7503513/1‑93 (MQ=255)

                    tactacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGaa                                                                     >  1:400501/1‑93 (MQ=255)

                    tactacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGaa                                                                     >  2:8606854/1‑93 (MQ=255)

                      ctacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAgg                                                                   >  2:7648633/1‑93 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAggg                                                                  >  1:2961856/1‑92 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgt                                                                  <  2:6312567/93‑1 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgt                                                                  >  1:7983199/1‑93 (MQ=255)

                       tacAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgt                                                                  >  1:230727/1‑93 (MQ=255)

                        acAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGCCCCCGCTGCCGCGCAACCGTGTTGTCCTAAAACTGGTGTTCCAGGAAGgtg                                                                 <  2:2031947/93‑1 (MQ=255)

                        acAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtg                                                                 <  1:6642360/93‑1 (MQ=255)

                          aGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtgtg                                                               >  1:12217/1‑93 (MQ=255)

                          aGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtgtg                                                               <  1:5014889/93‑1 (MQ=255)

                           gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtgtgt                                                              <  2:5838094/93‑1 (MQ=255)

                           gCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGgtgtgt                                                              <  1:9129236/93‑1 (MQ=255)

                            cGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTc                                                             <  1:9676699/93‑1 (MQ=255)

                             gTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCGGCGCAACCGTGTTGGCCTAAAACTGGTGTTCGAGGAAGGTGTGTCa                                                            >  1:6466853/1‑93 (MQ=255)

                             gTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCa                                                            <  1:5271613/93‑1 (MQ=255)

                              tCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAg                                                           >  1:2823247/1‑93 (MQ=255)

                                  tAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGa                                                       <  1:5521083/93‑1 (MQ=255)

                                   aaaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaa                                                      >  2:2279274/1‑93 (MQ=255)

                                    aaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaaa                                                     >  1:388196/1‑93 (MQ=255)

                                    aaTATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGaaa                                                     <  1:3760275/93‑1 (MQ=255)

                                      tataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATc                                                   >  2:4679482/1‑93 (MQ=255)

                                      tataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATc                                                   >  1:1978827/1‑93 (MQ=255)

                                      tataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATc                                                   >  2:6228567/1‑93 (MQ=255)

                                       ataGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATcc                                                  <  2:81885/93‑1 (MQ=255)

                                         aGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAg                                                <  2:9939597/93‑1 (MQ=255)

                                         aGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAg                                                >  1:1619990/1‑93 (MQ=255)

                                          gcgcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGc                                               <  2:2757106/93‑1 (MQ=255)

                                            gcCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCaa                                             >  2:3670030/1‑93 (MQ=255)

                                              cAGCGTAAAAGCTGTCGTGCCCCCGCTGCCGCGCAACCGTGTTGGCCTAAATCTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAAt                                           <  2:8834290/93‑1 (MQ=255)

                                              cAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAAt                                           >  2:2425540/1‑93 (MQ=255)

                                               aGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAAtt                                          >  2:966745/1‑93 (MQ=255)

                                                gCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTa                                         <  1:9963166/93‑1 (MQ=255)

                                                 cGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTaa                                        <  1:8194131/93‑1 (MQ=255)

                                                 cGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTaa                                        <  1:1470152/93‑1 (MQ=255)

                                                  gTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAAc                                       >  1:8348073/1‑93 (MQ=255)

                                                   tAAAAGCTGTCTGTACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACa                                      <  1:9313127/93‑1 (MQ=255)

                                                   tAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACa                                      >  2:4446155/1‑93 (MQ=255)

                                                   tAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACa                                      >  2:9770648/1‑93 (MQ=255)

                                                   tAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACa                                      >  1:5404232/1‑93 (MQ=255)

                                                   tAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACa                                      <  2:3967984/93‑1 (MQ=255)

                                                      aaGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttg                                   <  2:6753805/93‑1 (MQ=255)

                                                      aaGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttg                                   >  1:7277672/1‑93 (MQ=255)

                                                      aaGCTGACGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttg                                   <  1:8967840/93‑1 (MQ=255)

                                                       aGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgt                                  >  1:1010565/1‑93 (MQ=255)

                                                       aGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgt                                  <  2:3399469/93‑1 (MQ=255)

                                                         cTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACAttgttg                                <  2:3108315/93‑1 (MQ=255)

                                                          tGTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTgg                               <  2:14962/93‑1 (MQ=255)

                                                           gTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGt                              >  1:6304752/1‑93 (MQ=255)

                                                           gTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGt                              >  1:2193434/1‑93 (MQ=255)

                                                           gTCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGt                              <  2:1636371/93‑1 (MQ=255)

                                                            tCGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTa                             >  2:2635410/1‑93 (MQ=255)

                                                             cGTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTaa                            <  2:9445992/93‑1 (MQ=255)

                                                              gTGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAc                           <  2:180447/93‑1 (MQ=255)

                                                               tGACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAAcc                          >  2:7761449/1‑93 (MQ=255)

                                                                gACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCa                         <  2:4873508/93‑1 (MQ=255)

                                                                gACCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCa                         <  1:7688702/93‑1 (MQ=255)

                                                                 aCCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                        >  1:512092/1‑93 (MQ=255)

                                                                 aCCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                        <  2:7224469/93‑1 (MQ=255)

                                                                 aCCCCGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCAt                        >  1:1845194/1‑93 (MQ=255)

                                                                  ccccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATg                       <  1:458840/93‑1 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     <  2:6099984/93‑1 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     >  2:9032626/1‑93 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     >  2:8512553/1‑93 (MQ=255)

                                                                    ccGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCt                     >  1:5671089/1‑93 (MQ=255)

                                                                     cGCTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCtt                    <  2:5337449/93‑1 (MQ=255)

                                                                       cTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTc                  >  2:827435/1‑93 (MQ=255)

                                                                       cTGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTc                  >  2:2973741/1‑93 (MQ=255)

                                                                        tGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTca                 <  1:3442644/93‑1 (MQ=255)

                                                                        tGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTca                 <  1:7743210/93‑1 (MQ=255)

                                                                        tGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTca                 <  1:7019732/93‑1 (MQ=255)

                                                                        tGCCGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGAGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTca                 >  1:7061423/1‑93 (MQ=255)

                                                                          ccGCGCAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcacc               <  2:6390731/93‑1 (MQ=255)

                                                                            gcgcAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTcaccac             <  2:6712528/93‑1 (MQ=255)

                                                                              gcAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcg           <  2:8551532/93‑1 (MQ=255)

                                                                               cAACCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcga          <  2:3807089/93‑1 (MQ=255)

                                                                                aaCCGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgac         >  2:2358168/1‑93 (MQ=255)

                                                                                  ccGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACcgacga       <  1:9499554/93‑1 (MQ=255)

                                                                                   cGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGaa      >  2:10037740/1‑93 (MQ=255)

                                                                                   cGTGTTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGaa      >  2:430101/1‑93 (MQ=255)

                                                                                   cGTGTTGGCCCAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGaa      <  1:2624356/93‑1 (MQ=255)

                                                                                     tgtTGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACt    >  1:2470155/1‑93 (MQ=255)

                                                                                       ttGGCCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGa  >  1:4512154/1‑93 (MQ=255)

AGAAAGGTCTGGAAGACTTCTACTACAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTGTTGACCTAAAACTGGTGTTCCAGGAAGGTGTGTCAGCTGAAATCCAGCAAATTAACATTGTTGGTAACCATGCTTTCACCACCGACGAACTGA  >  NC_000913/198328‑198507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`