BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,191	T→C	R325R (CGT→CGC)	b1695 →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,191	0	T	C	99.1%	675.6 / ‑2.8	215	R325R (CGT→CGC)	b1695	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (114/99); total (116/99)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 218 total aligned reads displayed.

CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776102‑1776280

cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        >  2:8680624/1‑93 (MQ=255)

 gcAACATGGGGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       <  1:2195778/93‑1 (MQ=255)

   aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  1:9679487/1‑93 (MQ=255)

     cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAt                                                                                   <  1:9133031/93‑1 (MQ=255)

     cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCCCAAt                                                                                   >  2:3120084/1‑93 (MQ=255)

      aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  <  2:9041008/93‑1 (MQ=255)

       tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  2:9594902/93‑1 (MQ=255)

       tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  2:7857586/93‑1 (MQ=255)

       tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  1:3464525/93‑1 (MQ=255)

        ggTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGa                                                                                <  2:1770652/93‑1 (MQ=255)

         gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               <  1:7162178/93‑1 (MQ=255)

           gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGt                                                                             <  1:1115156/93‑1 (MQ=255)

             tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           <  1:8351654/93‑1 (MQ=255)

             tGAAAGGGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           <  2:2150254/93‑1 (MQ=255)

               aaaGTGCCACGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  2:1959721/93‑1 (MQ=255)

                aaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtg                                                                        >  2:4183547/1‑93 (MQ=255)

                 aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  2:9351584/93‑1 (MQ=255)

                   gggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatg                                                                     <  1:8501578/92‑1 (MQ=255)

                    ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  1:1575780/93‑1 (MQ=255)

                    ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    >  2:6251519/1‑93 (MQ=255)

                    ggcacggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  2:8147655/93‑1 (MQ=255)

                     gcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  1:9021944/93‑1 (MQ=255)

                     gcatgacaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  2:5652850/93‑1 (MQ=255)

                      catggcaAGCGGATCAGAATCAGTCGCTGCGCCCCAGCGGGGCGCTGGCAAAACTGTCCTGTGCACGCACCGCAATGGAAGTCATTGatgatg                                                                  <  2:2643183/93‑1 (MQ=255)

                       atgccaACCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 <  2:8452783/93‑1 (MQ=255)

                        tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  2:7981057/1‑93 (MQ=255)

                        tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  2:7920649/1‑93 (MQ=255)

                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               <  1:6024850/93‑1 (MQ=255)

                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               >  2:7291045/1‑93 (MQ=255)

                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               >  2:6061564/1‑93 (MQ=255)

                         ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               <  1:482188/93‑1 (MQ=255)

                          gcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAt                                                              >  2:3879283/1‑93 (MQ=255)

                            aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  1:3868768/1‑93 (MQ=255)

                               ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         >  2:4974963/1‑93 (MQ=255)

                                 gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       >  1:5500011/1‑93 (MQ=255)

                                 gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       >  1:1056978/1‑93 (MQ=255)

                                 gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       <  1:8042727/93‑1 (MQ=255)

                                  aTCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACCGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGTTTCAAATCa                                                      >  2:6960421/1‑93 (MQ=255)

                                   tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:3847055/1‑93 (MQ=255)

                                   tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:570866/1‑93 (MQ=255)

                                   tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     <  1:1262804/93‑1 (MQ=255)

                                   cgaGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     <  1:5058750/91‑1 (MQ=255)

                                    cAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATg                                                    <  1:6218937/93‑1 (MQ=255)

                                     aGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATgg                                                   <  2:4870334/93‑1 (MQ=255)

                                      gCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATggg                                                  <  1:2121465/93‑1 (MQ=255)

                                      gCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATggg                                                  <  1:2350534/93‑1 (MQ=255)

                                       cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 >  2:1257539/1‑93 (MQ=255)

                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:6361565/1‑93 (MQ=255)

                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:9222286/1‑93 (MQ=255)

                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:2256722/1‑93 (MQ=255)

                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  1:8190384/1‑93 (MQ=255)

                                         tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:8341353/1‑93 (MQ=255)

                                            gTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCt                                            >  2:2727736/1‑93 (MQ=255)

                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       >  2:3682947/1‑93 (MQ=255)

                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       >  2:2286465/1‑93 (MQ=255)

                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       >  2:6701875/1‑93 (MQ=255)

                                                 tGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCt                                       <  1:7486961/93‑1 (MQ=255)

                                                  gcgcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCta                                      >  2:4722592/1‑93 (MQ=255)

                                                   cgcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtat                                     <  2:3923582/93‑1 (MQ=255)

                                                     cACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAc                                   <  1:324245/93‑1 (MQ=255)

                                                     cACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAc                                   <  2:2120475/93‑1 (MQ=255)

                                                        cAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGa                                >  2:1677121/1‑93 (MQ=255)

                                                          gcgcGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATg                              <  2:8866367/93‑1 (MQ=255)

                                                          gcgcGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATg                              <  1:5909248/93‑1 (MQ=255)

                                                            gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            <  1:235627/93‑1 (MQ=255)

                                                            gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            >  2:7547295/1‑93 (MQ=255)

                                                             cggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCCGCGCTATACAGATGAgg                           >  2:9780547/1‑93 (MQ=255)

                                                              ggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgc                          >  2:7093182/1‑93 (MQ=255)

                                                               gcgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcg                         >  2:3487865/1‑93 (MQ=255)

                                                               gcgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcg                         <  1:1936907/93‑1 (MQ=255)

                                                                cgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgc                        >  2:7587910/1‑93 (MQ=255)

                                                                cgcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgc                        <  2:6579062/93‑1 (MQ=255)

                                                                 gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       <  1:2081934/93‑1 (MQ=255)

                                                                 gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       <  1:9888957/93‑1 (MQ=255)

                                                                  cTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcgc                      >  1:7325954/1‑93 (MQ=255)

                                                                   tGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGcgcgcg                     >  1:296975/1‑93 (MQ=255)

                                                                    ggCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGt                    <  2:1985357/93‑1 (MQ=255)

                                                                    ggCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGt                    <  1:8873637/93‑1 (MQ=255)

                                                                     gCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtc                   >  1:864698/1‑93 (MQ=255)

                                                                      cAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtct                  <  2:4001161/93‑1 (MQ=255)

                                                                       aaaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtctc                 <  1:7394901/93‑1 (MQ=255)

                                                                       aaaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtctc                 >  1:1752366/1‑93 (MQ=255)

                                                                        aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                <  1:3672234/93‑1 (MQ=255)

                                                                        aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                >  1:6856521/1‑93 (MQ=255)

                                                                        aaaCTGTACTGTGCACGCACCGCAATGGAAGACATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGAGCGTCTcc                >  1:4401112/1‑93 (MQ=255)

                                                                         aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               <  2:5282316/93‑1 (MQ=255)

                                                                         aaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTccc               >  1:6606368/1‑93 (MQ=255)

                                                                          actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  1:9404640/93‑1 (MQ=255)

                                                                          actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  1:1094348/93‑1 (MQ=255)

                                                                            tgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt            >  1:4131953/1‑93 (MQ=255)

                                                                            tgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt            >  2:2481384/1‑93 (MQ=255)

                                                                              tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          >  2:1432151/1‑93 (MQ=255)

                                                                                 tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       <  1:3982579/93‑1 (MQ=255)

                                                                                 tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       >  2:1513447/1‑93 (MQ=255)

                                                                                  gtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGc      >  2:5403996/1‑93 (MQ=255)

                                                                                  gtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGc      >  2:9115146/1‑93 (MQ=255)

                                                                                   tgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg     >  1:5265743/1‑93 (MQ=255)

                                                                                   tgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCg     >  2:7082291/1‑93 (MQ=255)

                                                                                     cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  1:5667757/1‑93 (MQ=255)

                                                                                      aCGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGACGAGGCGCGCGTCTCCCGCTTCTGGCGTGa  >  2:3255825/1‑93 (MQ=255)

CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776102‑1776280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`