BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,858,325	A→G	D344D (GAT→GAC)	b1776 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,858,325	0	A	G	99.7%	955.0 / ‑5.3	299	D344D (GAT→GAC)	b1776	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (141/157); total (142/157)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 310 total aligned reads displayed.

GTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAA  >  NC_000913/1858235‑1858414

   ttGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAc                                                                                      <  2:9875025/93‑1 (MQ=255)

   ttGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAc                                                                                      >  2:3188157/1‑93 (MQ=255)

    tGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGAcc                                                                                     <  1:540909/93‑1 (MQ=255)

     gTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCa                                                                                    <  1:8766836/93‑1 (MQ=255)

     gTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCa                                                                                    >  1:2175286/1‑93 (MQ=255)

      ttATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCAt                                                                                   >  1:2675502/1‑93 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  >  1:7065617/1‑93 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  <  2:2962916/93‑1 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  >  1:4380616/1‑93 (MQ=255)

       tATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATc                                                                                  >  1:9816164/1‑93 (MQ=255)

        aTCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCg                                                                                 >  1:10000159/1‑93 (MQ=255)

         tCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGc                                                                                <  1:7386983/93‑1 (MQ=255)

         tCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGc                                                                                <  1:1648864/93‑1 (MQ=255)

           tGTTCCATGCGATAATGTTCCTTCAGGAGAGAGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCAt                                                                              >  2:7572820/1‑93 (MQ=255)

           tGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCAt                                                                              >  1:7815473/1‑93 (MQ=255)

            gTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATc                                                                             >  1:4699942/1‑93 (MQ=255)

              tCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCaa                                                                           <  2:7167050/93‑1 (MQ=255)

                 aTGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAcc                                                                        >  1:756085/1‑93 (MQ=255)

                 aTGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAcc                                                                        <  2:5052291/93‑1 (MQ=255)

                  tGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAAccc                                                                       >  1:5682193/1‑93 (MQ=255)

                   gCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCt                                                                      <  2:6863718/93‑1 (MQ=255)

                    cGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCtt                                                                     <  1:1210828/93‑1 (MQ=255)

                    cGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCtt                                                                     >  2:2659294/1‑93 (MQ=255)

                     gATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTc                                                                    >  2:5285250/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCTACCATCGCATCAAACCCTTcg                                                                   >  1:7924456/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcg                                                                   >  2:5916004/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcg                                                                   >  2:4855217/1‑93 (MQ=255)

                      aTAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcg                                                                   >  1:3274679/1‑93 (MQ=255)

                       tAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcgc                                                                  >  2:3920769/1‑93 (MQ=255)

                       tAACGTTCCTTCAGGAGAGTGGGTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTcgc                                                                  >  2:7316550/1‑93 (MQ=255)

                           gTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCa                                                              <  1:9632779/93‑1 (MQ=255)

                           gTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCa                                                              >  2:1709989/1‑93 (MQ=255)

                            ttCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAt                                                             <  2:4223251/93‑1 (MQ=255)

                            ttCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAt                                                             >  1:8453375/1‑93 (MQ=255)

                            tgtcTTCAGTAGAGTGTTTAATCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAt                                                             <  1:7722150/90‑1 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            <  1:2878314/93‑1 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            >  2:2530898/1‑93 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            >  1:8349154/1‑93 (MQ=255)

                             tCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGCTTTGTCGACCATCGCATCAAACCCTTCGCGCCAtt                                                            >  2:2770906/1‑93 (MQ=255)

                              ccTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTg                                                           >  2:5729165/1‑93 (MQ=255)

                                  cAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGAc                                                       <  2:6784499/93‑1 (MQ=255)

                                  cAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGAc                                                       >  1:7642577/1‑93 (MQ=255)

                                   aGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACa                                                      <  2:8539433/93‑1 (MQ=255)

                                   aGGAGAGTGCTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACGATCGCATCAAACCCTTCGCGCCATTTCGACa                                                      <  2:799337/93‑1 (MQ=255)

                                     gagaGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAgg                                                    >  2:3339018/1‑93 (MQ=255)

                                     gagaGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAgg                                                    <  2:3553907/93‑1 (MQ=255)

                                     gagaGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAgg                                                    >  2:8078866/1‑93 (MQ=255)

                                      ggagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   <  1:9445892/92‑1 (MQ=255)

                                      agagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   >  1:3457193/1‑93 (MQ=255)

                                      agagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   >  2:4821543/1‑93 (MQ=255)

                                      agagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGc                                                   <  1:5057493/93‑1 (MQ=255)

                                       gagTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGcc                                                  >  1:7156641/1‑93 (MQ=255)

                                          tGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGAt                                               >  1:9423903/1‑93 (MQ=255)

                                           ggTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATa                                              >  1:1676733/1‑93 (MQ=255)

                                             ttATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACg                                            <  2:9665600/93‑1 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           <  2:748688/93‑1 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           <  1:3070988/93‑1 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           >  1:2240610/1‑93 (MQ=255)

                                              tATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGa                                           <  1:1936052/93‑1 (MQ=255)

                                               aTTCATCAAATTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCAACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGAt                                          <  2:9520962/93‑1 (MQ=255)

                                                  catcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCg                                       >  1:8692708/1‑93 (MQ=255)

                                                  catcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCg                                       <  1:2945500/93‑1 (MQ=255)

                                                   atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt                                      <  1:1085140/93‑1 (MQ=255)

                                                   atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt                                      <  2:937807/93‑1 (MQ=255)

                                                   atcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGt                                      >  2:6033394/1‑93 (MQ=255)

                                                    tcaAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTg                                     >  2:9685027/1‑93 (MQ=255)

                                                     caAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGa                                    >  1:1545227/1‑93 (MQ=255)

                                                     caAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGa                                    <  1:3925016/93‑1 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   <  2:4073280/93‑1 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   >  2:8180657/1‑93 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   >  1:7093772/1‑93 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGat                                   <  1:9578815/93‑1 (MQ=255)

                                                      aaaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTAat                                   <  1:8931315/93‑1 (MQ=255)

                                                       aaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatc                                  <  2:3671692/93‑1 (MQ=255)

                                                       aaGTCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatc                                  <  1:2098502/93‑1 (MQ=255)

                                                         gTCGTAAGTCATGATCGCTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatcat                                <  1:6351399/93‑1 (MQ=255)

                                                          tCGTAAGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGatcatc                               >  2:6410916/1‑93 (MQ=255)

                                                              aaGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGtt                           <  1:1791098/93‑1 (MQ=255)

                                                               aGTCATGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGttt                          <  2:7844491/93‑1 (MQ=255)

                                                                   aTGATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACt                      <  1:6101010/93‑1 (MQ=255)

                                                                     gATCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACttt                    <  2:2614212/93‑1 (MQ=255)

                                                                      aTCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTg                   >  2:9327842/1‑93 (MQ=255)

                                                                       tCACTTTGCTGGCGGTTTTGTCGACCATCACATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCTTCGGTTTGACTTTGa                  <  2:1004573/93‑1 (MQ=255)

                                                                       tCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa                  >  2:1141831/1‑93 (MQ=255)

                                                                       tCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa                  >  2:1948278/1‑93 (MQ=255)

                                                                       tCACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGa                  <  1:2773041/93‑1 (MQ=255)

                                                                        cACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAt                 >  2:4000645/1‑93 (MQ=255)

                                                                        cACTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGAt                 <  2:8739374/93‑1 (MQ=255)

                                                                          cTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAg               >  1:5165519/1‑93 (MQ=255)

                                                                          cTTTGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAg               <  1:373179/93‑1 (MQ=255)

                                                                           tttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgc              <  1:8618143/93‑1 (MQ=255)

                                                                           tttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgc              >  2:8399456/1‑93 (MQ=255)

                                                                           tttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgc              >  1:8548638/1‑93 (MQ=255)

                                                                            ttGATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAgcg             >  2:9287585/1‑93 (MQ=255)

                                                                              gATTGCGGTTTTGTCGACCATCGCATCAACCCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGcgcc           <  1:6908956/93‑1 (MQ=255)

                                                                              gATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGcgcc           >  1:9385076/1‑93 (MQ=255)

                                                                              gATTGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGcgcc           <  1:4968968/93‑1 (MQ=255)

                                                                                 tGCGGTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCt        <  1:5775172/93‑1 (MQ=255)

                                                                                  gCGGTTTTGTCGCCCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTg       <  2:4641448/93‑1 (MQ=255)

                                                                                    ggTTTTGTCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGc     >  2:135544/1‑93 (MQ=255)

GTTTTGTTATCTGTTCCATGCGATAACGTTCCTTCAGGAGAGTGGTTATTCATCAAAGTCGTAAGTCATGATCACTTTGATTGCGGTTTTATCGACCATCGCATCAAACCCTTCGCGCCATTGCGACAGGCCGATACGATGCGTGATCATCGGTTTGACTTTGATAGCGCCGCTGGCTAA  >  NC_000913/1858235‑1858414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`