BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,795	T→C	S60G (AGT→GGT)	yeaR ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,877,795	0	T	C	99.6%	804.8 / NA	250	S60G (AGT→GGT)	yeaR	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (123/125); minor base G (0/1); total (124/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

cagtcaTTGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                         <  1:31099/93‑1 (MQ=255)

cagtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                         <  1:31097/93‑1 (MQ=255)

cagtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgt                                                                                         >  2:2808733/1‑93 (MQ=255)

 agtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACcgtg                                                                                        >  1:5058838/1‑93 (MQ=255)

  gtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCgtgt                                                                                       >  2:9838146/1‑93 (MQ=255)

  gtcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCgtgt                                                                                       <  1:4469788/93‑1 (MQ=255)

   tcaTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGtt                                                                                      >  1:7994393/1‑93 (MQ=255)

    caTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtc                                                                                     >  1:7244201/1‑93 (MQ=255)

    caTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtc                                                                                     <  1:7534647/93‑1 (MQ=255)

    caTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtc                                                                                     <  1:10012711/93‑1 (MQ=255)

     aTGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGttaa                                                                                    >  1:2526390/1‑91 (MQ=255)

      tGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcat                                                                                   >  1:9307441/1‑93 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                  <  1:7910997/93‑1 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                  <  1:6154088/93‑1 (MQ=255)

       ggCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatc                                                                                  <  1:3942920/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCTCCTGATCAGGCTCTGCACCGTGTtcatca                                                                                 <  1:3385212/93‑1 (MQ=255)

        gCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTtcatca                                                                                 >  2:1073394/1‑93 (MQ=255)

          ttCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGc                                                                               >  1:6289682/1‑93 (MQ=255)

           tCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCg                                                                              >  2:5594564/1‑93 (MQ=255)

           tCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCg                                                                              >  1:2725954/1‑93 (MQ=255)

           tCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCg                                                                              >  1:2725957/1‑93 (MQ=255)

            cAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGt                                                                             <  1:3719605/93‑1 (MQ=255)

            cAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGt                                                                             >  2:2965623/1‑93 (MQ=255)

             aaTGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTa                                                                            >  1:8398900/1‑93 (MQ=255)

               tgttgtGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGc                                                                          >  1:2483580/1‑93 (MQ=255)

                 ttgtGCCACTTTTCTGGAGGGAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCg                                                                        <  2:3440774/93‑1 (MQ=255)

                     gCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGat                                                                    >  1:662408/1‑93 (MQ=255)

                      ccACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGata                                                                   >  2:6358404/1‑93 (MQ=255)

                      ccACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGata                                                                   <  2:7961361/93‑1 (MQ=255)

                       cACTTTTCTGGAGGGATCACCGCAAACTGCCCCGCTTCGATAAGGATCAGCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGatat                                                                  <  2:5223327/93‑1 (MQ=255)

                         cTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATAttt                                                                <  1:9141692/93‑1 (MQ=255)

                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                               >  2:1199148/1‑93 (MQ=255)

                          ttttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGCATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTg                                                               >  1:415475/1‑93 (MQ=255)

                           tttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGa                                                              <  2:1539027/93‑1 (MQ=255)

                           tttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGa                                                              <  1:2965623/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  2:3823636/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  1:6396817/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  2:2725957/93‑1 (MQ=255)

                            ttCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGAc                                                             <  2:2725954/93‑1 (MQ=255)

                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                          >  1:5280772/1‑93 (MQ=255)

                               tGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGc                                                          >  2:6709842/1‑93 (MQ=255)

                                 gAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGccc                                                        >  1:7671841/1‑93 (MQ=255)

                                 gAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATA‑TTGACCGcccc                                                       >  1:8764429/1‑93 (MQ=255)

                                  aGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGcccc                                                       <  2:7472402/93‑1 (MQ=255)

                                   gggAACATCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCa                                                      <  2:767754/93‑1 (MQ=255)

                                    ggAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCAt                                                     <  2:2076972/93‑1 (MQ=255)

                                     gAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATg                                                    >  1:8100678/1‑93 (MQ=255)

                                      aaCACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGc                                                   <  2:5819476/93‑1 (MQ=255)

                                       acacCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCa                                                  >  1:4812899/1‑93 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                               >  2:8858881/1‑93 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                               <  2:3278365/93‑1 (MQ=255)

                                          ccGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATaa                                               >  2:4332816/1‑93 (MQ=255)

                                           cGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAAc                                              <  1:5314333/93‑1 (MQ=255)

                                            gCAAACTGCCCCGCTTCGATAAGGATCGCCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACg                                             <  1:9785531/93‑1 (MQ=255)

                                             cAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACgg                                            <  2:7388699/93‑1 (MQ=255)

                                              aaaCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGa                                           <  1:4409553/93‑1 (MQ=255)

                                                aCTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGaaa                                         >  2:6472312/1‑93 (MQ=255)

                                                 cgtccccGCTTCGATAAGGAGCACCTGATCAGTCTCTGCACCGTGGTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                        <  1:9888385/90‑1 (MQ=255)

                                                 cTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                        <  2:9259796/93‑1 (MQ=255)

                                                 cTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAg                                        <  2:9332530/93‑1 (MQ=255)

                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                      >  2:5169108/1‑93 (MQ=255)

                                                   gCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGc                                      <  1:1823622/93‑1 (MQ=255)

                                                    ccccGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCg                                     <  2:7330746/93‑1 (MQ=255)

                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTgg                                  <  1:8476504/93‑1 (MQ=255)

                                                       cGCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTgg                                  <  1:4340414/93‑1 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCGTAACGGAAAGGCGTggg                                 <  1:6886325/93‑1 (MQ=255)

                                                        gCTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTggg                                 <  2:2548643/93‑1 (MQ=255)

                                                         cTTCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGt                                >  2:8953278/1‑93 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                               >  1:6234476/1‑93 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                               <  2:1738087/93‑1 (MQ=255)

                                                          ttCGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTa                               >  2:4481906/1‑93 (MQ=255)

                                                            cGATAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTaaa                             <  2:6265143/93‑1 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCTGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           >  1:6550138/1‑93 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           <  2:2238534/93‑1 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           >  2:5504819/1‑93 (MQ=255)

                                                              aTAAGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcc                           >  2:9663101/1‑93 (MQ=255)

                                                                aaGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAcccc                         <  1:9443839/93‑1 (MQ=255)

                                                                 aGGATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAAccccc                        <  1:2184542/93‑1 (MQ=255)

                                                                   gATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCgg                      <  1:1065921/93‑1 (MQ=255)

                                                                   gATCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCgg                      <  1:8150519/93‑1 (MQ=255)

                                                                    aTCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCggg                     <  2:482365/93‑1 (MQ=255)

                                                                     tCACCTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGgc                    >  2:8446264/1‑93 (MQ=255)

                                                                        ccTGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGcgcg                 >  1:5980276/1‑93 (MQ=255)

                                                                          tGATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGtt               <  1:52421/93‑1 (MQ=255)

                                                                           gATCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTc              >  1:5943039/1‑93 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTcc             >  2:1523970/1‑93 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTcc             <  1:4618448/93‑1 (MQ=255)

                                                                            aTCAGGCTCTGCACCGTGTGCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTcc             <  2:7833575/93‑1 (MQ=255)

                                                                             tCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCt            >  1:145984/1‑93 (MQ=255)

                                                                             tCAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGGTCCt            >  1:1501055/1‑93 (MQ=255)

                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCtt           >  2:6735665/1‑93 (MQ=255)

                                                                              cAGGCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCACGGGCGCGCTCCtt           >  2:4275623/1‑93 (MQ=255)

                                                                               cggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCttt          <  2:8386767/92‑1 (MQ=255)

                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTa         <  1:745224/93‑1 (MQ=255)

                                                                                ggCTCTGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTa         <  2:6593568/93‑1 (MQ=255)

                                                                                     tGCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAg    >  2:3549013/1‑93 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGa   <  2:7188783/93‑1 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGa   <  1:3648331/93‑1 (MQ=255)

                                                                                      gCACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGa   >  2:10035857/1‑93 (MQ=255)

                                                                                       cACCGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAt  <  2:937958/93‑1 (MQ=255)

CAGTCATGGCTTCAATGTTGTGCCACTTTTCTGGAGGGAACACCGCAAACTGCCCCGCTTCGATAAGGATCACCTGATCAGGCTCTGCACTGTGTTCATCAGCGTAGCCGAGATATTTGACCGCCCCATGCATAACGGAAAGGCGTGGGTAAACCCCCGGGCGCGTTCCTTTATCAAGAT  >  NC_000913/1877705‑1877884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`