BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,768	A→G	G336G (GGT→GGC)	proS ←	proline tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,768	0	A	G	95.2%	900.1 / 22.2	311	G336G (GGT→GGC)	proS	proline tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (8/7); new base G (164/131); total (172/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 320 total aligned reads displayed.

ATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAAC  >  NC_000913/217678‑217857

aTCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGcc                                                                                         <  1:4332479/93‑1 (MQ=255)

aTCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGcc                                                                                         <  2:8615407/93‑1 (MQ=255)

 acaaTGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGccc                                                                                        <  2:6863975/90‑1 (MQ=255)

  cGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCg                                                                                       <  2:7486762/93‑1 (MQ=255)

   gATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCgg                                                                                      >  1:584023/1‑93 (MQ=255)

    aTGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGa                                                                                     >  2:8274291/1‑93 (MQ=255)

     tGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGAc                                                                                    <  1:7738237/93‑1 (MQ=255)

      gTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGAcc                                                                                   >  1:2277751/1‑93 (MQ=255)

         aGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGc                                                                                >  1:3981694/1‑93 (MQ=255)

           cACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCtt                                                                              <  1:1627932/93‑1 (MQ=255)

             ccAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTa                                                                            >  2:9522062/1‑93 (MQ=255)

              cagcagCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTaa                                                                           >  2:8553163/1‑93 (MQ=255)

              cagcagCGAAATCACTCATCGCCGCAACGGTACAGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTaa                                                                           <  1:9684426/93‑1 (MQ=255)

               agcagcGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAAc                                                                          <  2:12230/93‑1 (MQ=255)

                gcagcGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAAcc                                                                         <  1:2899873/93‑1 (MQ=255)

                gcagcGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAAcc                                                                         <  2:535408/93‑1 (MQ=255)

                  agcGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCAc                                                                       >  1:1481893/1‑93 (MQ=255)

                  agcGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCAc                                                                       <  2:4002225/93‑1 (MQ=255)

                     gAAATCACTCATCGCCGCAACGGTACGGTCAATCCCCACCGGAATCGGCATGTTTACCGGACCCAGTGAGTCCGGACCGGCTTTAACCACGGc                                                                    >  2:3481515/1‑93 (MQ=255)

                      aaaTCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCa                                                                   >  1:1107858/1‑93 (MQ=255)

                         tCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGa                                                                <  2:5118178/93‑1 (MQ=255)

                          cACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGaa                                                               >  1:8142558/1‑93 (MQ=255)

                           aCTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAAt                                                              >  2:7378783/1‑93 (MQ=255)

                           aCTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAAt                                                              >  1:6428423/1‑93 (MQ=255)

                            cTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAAtt                                                             >  1:5056777/1‑93 (MQ=255)

                            cTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAAtt                                                             <  1:6956701/93‑1 (MQ=255)

                             tCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAAttt                                                            >  1:320281/1‑93 (MQ=255)

                             tCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAAttt                                                            <  1:6214438/93‑1 (MQ=255)

                              cATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttc                                                           >  1:5483880/1‑93 (MQ=255)

                               aTCGCCGCAACGGTACGGTCAATCACCACCGGAATCCGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttct                                                          <  2:3655755/93‑1 (MQ=255)

                                tCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttctt                                                         >  1:9034360/1‑93 (MQ=255)

                                tCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttctt                                                         <  1:7509557/93‑1 (MQ=255)

                                tCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttctt                                                         <  1:332906/93‑1 (MQ=255)

                                 cgccgcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttcttc                                                        <  1:1350836/93‑1 (MQ=255)

                                  gccgcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttcttct                                                       <  1:4895327/93‑1 (MQ=255)

                                  gccgcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttcttct                                                       <  1:1866900/93‑1 (MQ=255)

                                   ccgcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttcttctt                                                      <  2:7949725/93‑1 (MQ=255)

                                    cgcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATttcttcttc                                                     <  2:6423073/93‑1 (MQ=255)

                                     gcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCg                                                    >  2:5709661/1‑93 (MQ=255)

                                     gcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCg                                                    >  1:988511/1‑93 (MQ=255)

                                     gcAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCg                                                    <  1:6779525/93‑1 (MQ=255)

                                      cAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCgg                                                   >  2:2614223/1‑93 (MQ=255)

                                        acggtacggtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTc                                                 >  1:6506771/1‑93 (MQ=255)

                                        acggtacggtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTc                                                 <  1:4470922/93‑1 (MQ=255)

                                            tacggtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGa                                             >  2:6228672/1‑93 (MQ=255)

                                            tacggtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGa                                             >  1:5710478/1‑93 (MQ=255)

                                               ggtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAg                                          >  1:8763421/1‑93 (MQ=255)

                                               ggtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAg                                          <  1:10030921/93‑1 (MQ=255)

                                                gtCAATCACCACCGGAATCGGCATGTTTACCGGACGCAGTGAGCCCGGACCGGCTTTAACAACGGCACGAATTTCTTCTTCGGGCGAGAAAGt                                         >  1:1765234/1‑93 (MQ=255)

                                                gtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGACTTTCTTCTTCGGTCGCGAAAgc                                         >  1:8387084/1‑92 (MQ=255)

                                                gtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGt                                         >  2:3329530/1‑93 (MQ=255)

                                                gtCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGt                                         >  1:656153/1‑93 (MQ=255)

                                                 tCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTc                                        <  2:1012482/93‑1 (MQ=255)

                                                 tCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTc                                        <  2:608690/93‑1 (MQ=255)

                                                 tCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTc                                        <  1:1938223/93‑1 (MQ=255)

                                                  cAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCa                                       >  1:2725465/1‑93 (MQ=255)

                                                    aTCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGc                                     >  1:5081733/1‑93 (MQ=255)

                                                     tCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCg                                    <  1:2199428/93‑1 (MQ=255)

                                                     tCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCg                                    <  1:6926101/93‑1 (MQ=255)

                                                     tCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCg                                    >  2:8109746/1‑93 (MQ=255)

                                                     tCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCg                                    <  2:4745002/93‑1 (MQ=255)

                                                       accaccGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCggg                                  >  1:9253375/1‑93 (MQ=255)

                                                         caccGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCt                                >  1:888912/1‑93 (MQ=255)

                                                            cGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGc                             <  2:4154407/93‑1 (MQ=255)

                                                            cGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGc                             >  1:4040639/1‑93 (MQ=255)

                                                            cGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGc                             <  2:2784500/93‑1 (MQ=255)

                                                            cGGAATCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGc                             <  1:880698/93‑1 (MQ=255)

                                                               aaTCGGCATGTTTACCGGACCCAGTGAGCCGGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAAc                          <  1:388945/93‑1 (MQ=255)

                                                               aaTCGGCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGAGGGCTTGCAAc                          >  1:7935399/1‑93 (MQ=255)

                                                                aTCGGGATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAAcc                         >  1:6061968/1‑93 (MQ=255)

                                                                 tCGGCATGTTTACCGGACGCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCt                        <  2:7005570/93‑1 (MQ=255)

                                                                   ggCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCCACCCGc                      >  2:2340268/1‑93 (MQ=255)

                                                                   ggCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGc                      >  2:1568961/1‑93 (MQ=255)

                                                                    gCATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCg                     <  2:9306492/93‑1 (MQ=255)

                                                                     cATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCgg                    <  1:827973/93‑1 (MQ=255)

                                                                     cATGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCgg                    >  1:3004559/1‑93 (MQ=255)

                                                                      aTGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGc                   >  2:9066051/1‑93 (MQ=255)

                                                                       tGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCa                  >  2:4933702/1‑93 (MQ=255)

                                                                       tGTTTACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCa                  >  2:8839657/1‑93 (MQ=255)

                                                                         tttACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTCCCGCAGt                >  1:595513/1‑93 (MQ=255)

                                                                          ttACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGtt               <  1:735517/93‑1 (MQ=255)

                                                                          ttACCGGACCCAGGGAGCCCGGCCCGGCTTTAACCACGGCACGAATTTCTTCTGCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGtt               <  2:1898210/93‑1 (MQ=255)

                                                                          gtACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGtt               <  2:1065976/92‑1 (MQ=255)

                                                                           tACCGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGttt              >  1:3613057/1‑93 (MQ=255)

                                                                             ccGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCGGttttt            >  2:976843/1‑93 (MQ=255)

                                                                             ccGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGttttt            <  2:8692099/93‑1 (MQ=255)

                                                                             ccGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGttttt            >  1:8567727/1‑93 (MQ=255)

                                                                             ccGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTCGCAACCTGCGGCAGttttt            <  2:2534637/93‑1 (MQ=255)

                                                                              cGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTc           >  2:775685/1‑93 (MQ=255)

                                                                              cGGACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTc           <  2:5466696/93‑1 (MQ=255)

                                                                               ggACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCt          >  2:662210/1‑93 (MQ=255)

                                                                                gACCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTg         <  1:7892047/93‑1 (MQ=255)

                                                                                 aCCCAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGc        >  1:9286910/1‑93 (MQ=255)

                                                                                   ccAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCtt      >  2:1355262/1‑93 (MQ=255)

                                                                                    cAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCttt     <  2:654087/93‑1 (MQ=255)

                                                                                    cAGTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCttt     >  1:346262/1‑93 (MQ=255)

                                                                                      gTGAGCCGGGACCGGCTTTAACCACGGCACGAATTTCTTCGTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTaa   <  1:9535206/93‑1 (MQ=255)

                                                                                      gTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTaa   >  1:6731596/1‑93 (MQ=255)

                                                                                      gTGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGATTTac   >  2:3091098/1‑92 (MQ=255)

                                                                                       tGAGCCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAAc  >  2:9479752/1‑93 (MQ=255)

ATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAAC  >  NC_000913/217678‑217857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`