BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,344	G→A	Q174Q (CAG→CAA)	yecD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,344	0	G	A	75.8%	1861.4 / inf	944	Q174Q (CAG→CAA)	yecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (108/120); new base A (357/358); total (466/478)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-02

Only 100 of 1003 total aligned reads displayed.

ATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTA  >  NC_000913/1949252‑1949436

  cGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAgcaaca                                                                                            <  2:227630/93‑1 (MQ=255)

    gtgtTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAAcaca                                                                                          <  2:739349/93‑1 (MQ=255)

     tgtTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACaa                                                                                         >  1:6353888/1‑93 (MQ=255)

     tgtTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACaa                                                                                         >  1:279659/1‑93 (MQ=255)

      gtTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAAt                                                                                        <  1:2952511/93‑1 (MQ=255)

       ttGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATa                                                                                       <  1:195332/93‑1 (MQ=255)

       ttGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATa                                                                                       >  1:6185339/1‑93 (MQ=255)

        tGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATaa                                                                                      <  2:1607519/93‑1 (MQ=255)

        tGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATaa                                                                                      <  1:1423050/93‑1 (MQ=255)

         gAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAAc                                                                                     <  1:4974356/93‑1 (MQ=255)

           aTCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAg                                                                                   <  2:2115257/93‑1 (MQ=255)

           aTCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAg                                                                                   <  2:3353113/93‑1 (MQ=255)

           aTCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAg                                                                                   <  1:228093/93‑1 (MQ=255)

            tCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAgc                                                                                  >  2:238328/1‑92 (MQ=255)

             ccACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAgca                                                                                 <  2:7675097/93‑3 (MQ=255)

               aCCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCAtt                                                                               <  2:2633517/93‑2 (MQ=255)

               aCCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCAtt                                                                               <  2:3906909/93‑2 (MQ=255)

                ccgcccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCAtta                                                                              >  1:2464848/1‑91 (MQ=255)

                    ccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCa                                                                          >  1:3934494/1‑93 (MQ=255)

                    ccgcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCa                                                                          >  1:4397901/1‑93 (MQ=255)

                      gcAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCata                                                                        <  2:4767100/93‑1 (MQ=255)

                          tGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATAATATCTa                                                                    >  2:9360483/1‑93 (MQ=255)

                           gCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCtcc                                                                   <  1:7469995/93‑3 (MQ=255)

                            ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTAcc                                                                  <  1:8247844/93‑1 (MQ=255)

                            ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTAcc                                                                  >  2:1349938/1‑93 (MQ=255)

                            ccTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTAcc                                                                  <  2:5476597/93‑1 (MQ=255)

                             cTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTAccc                                                                 >  2:5248556/1‑93 (MQ=255)

                              tGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCcg                                                                <  2:5609086/93‑1 (MQ=255)

                                ggAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCcgcg                                                              >  2:9011245/1‑93 (MQ=255)

                                   aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCAt                                                           >  1:8776070/1‑93 (MQ=255)

                                   aCTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCAt                                                           >  2:2515994/1‑93 (MQ=255)

                                   aCTTGGCTTTAACCT‑GTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                          <  1:4195805/93‑1 (MQ=255)

                                    cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATc                                                          >  1:4731925/1‑93 (MQ=255)

                                    cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATc                                                          <  2:455219/93‑1 (MQ=255)

                                    cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                          >  2:6282607/1‑93 (MQ=255)

                                    cTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATc                                                          <  1:9475399/93‑1 (MQ=255)

                                     ttGGCTTTAATCTGGTGATCGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCg                                                         >  1:3168531/1‑93 (MQ=255)

                                      tGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGc                                                        <  1:8909825/93‑1 (MQ=255)

                                      tGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGc                                                        >  1:9576600/1‑93 (MQ=255)

                                      tGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGc                                                        >  2:7268222/1‑93 (MQ=255)

                                      tGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGc                                                        <  1:7918163/93‑1 (MQ=255)

                                       ggCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGcc                                                       >  2:3589653/1‑93 (MQ=255)

                                        gCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGccc                                                      >  1:9864521/1‑93 (MQ=255)

                                          tttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCgt                                                    >  1:8689532/1‑93 (MQ=255)

                                           ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtg                                                   <  2:1220063/93‑1 (MQ=255)

                                           ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtg                                                   <  1:10003588/93‑1 (MQ=255)

                                           ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtg                                                   <  2:684114/93‑1 (MQ=255)

                                           ttAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtg                                                   >  1:5300136/1‑93 (MQ=255)

                                           ttAATCTGGGGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCgtg                                                   <  1:6849152/93‑1 (MQ=255)

                                            tAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCgtgt                                                  <  2:4316032/93‑1 (MQ=255)

                                                cTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGt                                              >  2:1873043/1‑93 (MQ=255)

                                                 tGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCTCCCGTGTGCGTa                                             <  1:1799034/93‑1 (MQ=255)

                                                  ggTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAg                                            <  2:6586382/93‑1 (MQ=255)

                                                  ggTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAg                                            >  2:6388033/1‑93 (MQ=255)

                                                  ggTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAg                                            >  1:6663969/1‑93 (MQ=255)

                                                  ggTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAg                                            <  2:5961131/93‑1 (MQ=255)

                                                     gATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGt                                         <  2:8258455/93‑1 (MQ=255)

                                                      aTTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTg                                        <  2:9807458/93‑1 (MQ=255)

                                                      aTTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTg                                        >  1:9312325/1‑93 (MQ=255)

                                                      aTTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCAGTGTGCGTAGCGTg                                        <  1:1727805/93‑1 (MQ=255)

                                                       ttGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTgg                                       >  2:1020161/1‑93 (MQ=255)

                                                         gCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGaa                                     >  1:5863040/1‑93 (MQ=255)

                                                         gCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGaa                                     <  2:8029043/93‑1 (MQ=255)

                                                         gCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGaa                                     <  1:8178985/93‑1 (MQ=255)

                                                           cGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAaga                                   <  1:5126485/93‑1 (MQ=255)

                                                             aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                 >  2:8433147/1‑93 (MQ=255)

                                                             aaGACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAgaga                                 >  2:3014422/1‑93 (MQ=255)

                                                              aGACGCCTGTCGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCTCGTGTGCGTAGCGTGGAAGAGAt                                >  2:7206579/1‑93 (MQ=255)

                                                               gACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                               >  1:728082/1‑93 (MQ=255)

                                                               gACGCCTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATc                               >  2:8721772/1‑93 (MQ=255)

                                                                aCGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcc                              <  2:7113845/93‑1 (MQ=255)

                                                                aCGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATcc                              >  1:551922/1‑93 (MQ=255)

                                                                   ccTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCa                           >  2:2907223/1‑93 (MQ=255)

                                                                   ccTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttc                           >  1:1549095/1‑91 (MQ=255)

                                                                   ccTGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttc                           <  1:4555264/93‑3 (MQ=255)

                                                                     tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                         >  2:9167385/1‑89 (MQ=255)

                                                                     tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                         >  2:3135623/1‑89 (MQ=255)

                                                                     tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                         >  1:3694087/1‑89 (MQ=255)

                                                                     tGTAGTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCttcac                         >  1:3289745/1‑89 (MQ=255)

                                                                        aGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAAcgcg                      >  2:5757152/1‑93 (MQ=255)

                                                                         gTGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGt                     >  1:7711696/1‑93 (MQ=255)

                                                                          tGCCGCCAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGtt                    >  1:7046824/1‑93 (MQ=255)

                                                                            ccgccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTAt                  <  2:4146237/93‑1 (MQ=255)

                                                                             cgccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATg                 >  2:1723808/1‑93 (MQ=255)

                                                                              gccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGa                <  1:1135337/93‑1 (MQ=255)

                                                                              gccAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGa                >  1:6018555/1‑93 (MQ=255)

                                                                              cccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGa                <  1:257221/92‑1 (MQ=255)

                                                                               ccAGCGCCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGAt               >  1:8453117/1‑93 (MQ=255)

                                                                                  gcgcCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTa            >  1:8600066/1‑93 (MQ=255)

                                                                                  gcgcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTa            >  1:1311490/1‑93 (MQ=255)

                                                                                    gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACa          <  1:2141956/93‑1 (MQ=255)

                                                                                    gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACa          <  2:6689481/93‑1 (MQ=255)

                                                                                    gcCGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACa          <  2:9466422/93‑1 (MQ=255)

                                                                                      cGAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATc        >  1:3522528/1‑93 (MQ=255)

                                                                                       gAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCg       >  2:5061514/1‑93 (MQ=255)

                                                                                       gAGCAACACAATAACAGCATTAATCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTTCACGCGTTATGATTTACATCg       <  2:9597119/93‑1 (MQ=255)

                                                                                        agcagcACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCgg      <  1:9166170/93‑1 (MQ=255)

                                                                                         gcagcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGt     <  2:7863409/93‑1 (MQ=255)

                                                                                         gcagcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGt     >  1:5669842/1‑93 (MQ=255)

                                                                                         gcagcaCAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGt     <  2:4989305/93‑1 (MQ=255)

ATCGGTGTTGAATCCACCGCCCGCAATGCCTGGGAACTTGGCTTTAATCTGGTGATTGCCGAAGACGCCTGTAGTGCCGCCAGCGCCGAGCAGCACAATAACAGTATCAACCATATCTACCCGCGCATCGCCCGTGTGCGTAGCGTGGAAGAGATCCTCAACGCGTTATGATTTACATCGGTCTA  >  NC_000913/1949252‑1949436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`